LOCUS       c00072_gnlPro..         4997 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IFKONMCM_72.
ACCESSION   c00072_gnlPro..
VERSION     c00072_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 20:46:47
            Original ID  :: gnl|Prokka|IFKONMCM_72
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4997
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..618
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg72_1"
                     /sec_met_domain="Thiolase_N (E-value: 1.1e-29, bitscore:
                     92.2, seeds: 30, tool: rule-based-clusters)"
                     /sec_met_domain="Thiolase_C (E-value: 2.4e-50, bitscore:
                     158.1, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DKAIKDGAFKDEIVPVVIKGKKGDTVFDTDEGPRLSAPEVLAKLK
                     PAFTKDGIVTAANSSAINDGAAALVIMSEEKAKELGVKPLATWVAGALAGVEPEVMGLG
                     PIAATKKVMAKTGLTVADMDLVEANEAFAAQSIAVGEALGFDKDKLNVNGGAIALGHPV
                     GASGARILVTLLYAMKHRGAHKGLATLCIGGGMGCATIVEMD"
     protocluster    1..4997
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:4996]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..4996
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..4997
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4997
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             743..1528
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: EC1_2-faecali"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg72_2"
                     /sec_met_domain="EC1_2-faecali (E-value: 8.8e-94, bitscore:
                     302.1, seeds: 67, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFVKTEVQGAVEIITIDRPKALNALNPEVLADLKAAFEAVDQET
                     VRCIVLTGEGDKSFVAGADIGSMSTMTKAEGEAFGKLGNDVFLMIEHFPIPVIAAVNGF
                     ALGGGNELAMSCDIRICSDNAVFGQPEVGLGITPGFGGTQRLARLVGMGMAKQLVYSAL
                     NIKADEAYRIGLVNAVYPQAELMENVLKLAGKIAKNAPIAVRNCKKAMNDGISLPIEKA
                     VEVEEKLFGDCFETHDQQEGMACFLSREKPKPKAVFTNN"
     CDS             1568..2440
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) F420_oxidored"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg72_3"
                     /sec_met_domain="HCDH (E-value: 4.6e-35, bitscore: 109.0,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 1.3e-61, bitscore: 196.4,
                     seeds: 60, tool: rule-based-clusters)"
                     /sec_met_domain="F420_oxidored (E-value: 3e-08, bitscore:
                     23.0, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIGAGTMGQGIAKAFAQVEGNTVALCDIKQEWAENGLAKIK
                     KGYEKLVAKGKMTQEKADAIVAAITPGLKEDLCADCDLVVEAAFEDMKVKQTTFGELDK
                     ICKPECVFASNTSSLSITEIGKGLSRPLIGMHFFNPADRMKLIEVIAGCNTPAETVEKI
                     KEISVAIGKSPVQVNEAAGFVVNRILIPMINEAAFIKMEGVSDIAGIDTAMKLGANHPM
                     GPLELGDFIGLDICLAIMDVLYNETGDSKYRACPLIRKMVRGGNLGCKTGKGFYVYNAD
                     RTKTPVDQL"
     CDS             2547..3716
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg72_4"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.8e-50, bitscore:
                     159.8, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 2.2e-30, bitscore:
                     93.5, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 6.4e-41, bitscore:
                     128.4, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFTLSKQQQMVQKMYREFAENEVKPLAKKVDAEEYFPKETVEKM
                     GKLGMMGIYFPTEVGGAGGDVLSYVMAVEELSKVCGTTGVIVSAHTSLCAAPIYENGTP
                     EQKAKYLPKLCSGEWLGAFGLTEPGAGTDAQGQQTIAKEDGDYWVLNGSKIFITNAGFA
                     DVFIVIAVTDNVLDKRGRPTKLCSAFIVERTDPGFSVGKAEDKMGIRGSSTCELIFEDC
                     RIPKDRMLGVRGKGFQLAMATLDGGRIGIASQALGIAEGALEETIAYVKERKQFGRAIA
                     AFQNTQFELAEMKARVEAAKYLVYAAALKKQQAMDGAKVRYSVEAAQAKLIAARTASDV
                     TRRCLQLFGGYGYTRDYPIERMMRDAKITEIYEGTSEVQMMVISGALLK"
     CDS             3734..4528
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 157;
                     E-value: 1.6e-48)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg72_5"
                     /sec_met_domain="ETF (E-value: 1.5e-49, bitscore: 157.0,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAIVCVKQVPDTSGKVSVKPDGTLDRASMATITNPDDLNALEAA
                     LKLKDATGCEVVVVTMGPPPAEGMLRELLARGADKAVLVSGREFGGSDTFATSQILAAA
                     VNKIGVGPEDVVFCGRQAIDGDTAQVGPQIAEKLHLPQVTYVADIQKDGNTLTVKRMLE
                     DGYMMVKVQTPCLLTCIKELNDPRYMSVNGIFTCYDKPMEVFDYNALKDDPLIEVDTIG
                     LKGSPTNVFKSFTPPQKGAGTMLQGDDVAAQLAGILAKKHLI"
     CDS             4550..4996
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 120.7;
                     E-value: 2.2e-37)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg72_6"
                     /sec_met_domain="ETF (E-value: 2.2e-38, bitscore: 120.7,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADFNEYKGVWVFCEQRQGALMSTDFELVSEARKLADELGCEVTG
                     LLLGDNVEGIAKELGGYGADKVLVCDSPLLKDYTTDAYAKVVCDMVNEYKPEVLLIGAT
                     NIGRDLGPRCAARLHTGLTADATHLDIDTAKYIDFLKESSTIDLS"
ORIGIN
        1 gacaaggcca tcaaggacgg cgctttcaag gatgagatcg ttcctgttgt catcaagggc
       61 aagaagggcg acaccgtgtt cgataccgat gaaggccccc gtctgagcgc tcccgaagtg
      121 ctggcaaagc tgaagcccgc cttcaccaag gacggcatcg tcaccgctgc aaactcctct
      181 gctatcaacg atggcgcagc tgctctggtc atcatgagcg aagagaaggc caaggagctg
      241 ggcgtgaagc ctctggctac ctgggtcgca ggtgctctgg caggcgtcga gcctgaggtc
      301 atgggtcttg gccccatcgc tgctaccaag aaggtcatgg ctaagaccgg tctgaccgtt
      361 gctgatatgg acctggtcga ggccaacgag gcattcgcag cacagtccat cgcagtcggc
      421 gaggctctgg gcttcgataa ggacaagctg aacgtcaacg gcggcgcaat cgctttgggt
      481 cacccggtcg gcgcttccgg cgctcgtatc ctggtcaccc tgctgtacgc tatgaagcac
      541 cgtggtgctc acaagggtct ggctactctg tgcatcggcg gcggcatggg ctgcgctacc
      601 atcgttgaga tggactaagc actctttctc tgaatcctga ttgaaccaca gacccggagc
      661 gccggagaga accggagagc cctcccgcgt cccgggggct gttttgaaaa aaactgaact
      721 gtatgaaatg gaggttttca caatggcatt tgtaaaaacc gaggtgcagg gcgctgttga
      781 gatcattacc atcgaccgtc ccaaggcact gaacgccctg aaccccgagg ttctggctga
      841 cctgaaggct gcttttgagg cagtcgatca ggagactgtc cgctgcatcg ttctgaccgg
      901 cgaaggcgac aagagcttcg ttgctggtgc tgatatcggt tctatgagca ccatgactaa
      961 ggcagaaggc gaggccttcg gcaagctggg caatgatgtg ttcctgatga tcgagcattt
     1021 ccccatcccc gtcatcgcag ccgtcaacgg ctttgcactg ggcggcggca acgagctggc
     1081 tatgagctgc gatatccgca tctgctctga caacgctgtg ttcggtcagc cggaagtcgg
     1141 tctgggcatc accccgggct tcggcggcac ccagcgtctg gctcgtctgg tcggcatggg
     1201 catggcaaag cagctggtct actctgcgct gaacatcaag gctgacgagg cttaccgcat
     1261 cggtctggtc aacgccgtgt atccgcaggc tgagctgatg gagaacgtcc tgaagctggc
     1321 cggcaagatc gcaaagaacg ctcccattgc tgtgcgtaac tgcaagaagg caatgaacga
     1381 cggcatcagc ctgcctatcg agaaggctgt tgaggtcgag gagaagctgt tcggcgattg
     1441 cttcgagacc cacgaccagc aggagggcat ggcctgcttc ctgtcccgtg agaaaccaaa
     1501 gcccaaggca gtgttcacca acaactaatc cattacataa tttttgatat taaaggagag
     1561 atttcctatg aagatcggtg ttatcggcgc tggcactatg ggtcagggca tcgcaaaggc
     1621 atttgcacag gttgagggca acaccgttgc tctgtgcgac atcaagcagg agtgggccga
     1681 gaatggtctg gcaaagatca agaagggcta cgagaagctg gtcgctaagg gaaagatgac
     1741 tcaggagaag gctgacgcaa tcgtcgccgc catcaccccg ggcctgaagg aagatctgtg
     1801 cgcagactgc gatctggtcg ttgaggctgc tttcgaggat atgaaggtca agcagaccac
     1861 cttcggcgag ctggataaga tctgcaagcc cgagtgcgtc ttcgcttcca acacttcttc
     1921 tctgtccatt accgagatcg gcaagggttt gtctcgtccc ctgatcggta tgcacttctt
     1981 caaccccgca gaccgtatga agctgatcga ggtcatcgcc ggctgcaaca ctcccgccga
     2041 gaccgttgag aagatcaagg agatctccgt tgccatcggc aagagccccg ttcaggtcaa
     2101 cgaggctgcc ggctttgtgg tcaaccgcat cctgatcccc atgatcaacg aggctgcctt
     2161 cattaagatg gagggcgttt ccgatatcgc cggcatcgac accgctatga agctgggcgc
     2221 taaccacccc atgggtcccc tggagctggg cgacttcatc ggtctggaca tctgcctggc
     2281 cattatggac gtgctgtaca acgagaccgg cgacagcaag taccgtgcct gcccgctgat
     2341 ccgcaagatg gtgcgcggcg gcaatctggg ctgcaagacc ggtaagggct tctacgttta
     2401 caacgcagac cgcaccaaga cccctgttga ccagctgtaa attctgataa acatttctcc
     2461 cggctgcggg gaaagcggtc gggaggaatt tgtttgcacc gggctattcc ggtaaatgaa
     2521 aaaagacctg ttaggaggat atagcgatgg attttactct gtccaagcag cagcagatgg
     2581 tgcagaaaat gtaccgcgag tttgctgaga acgaagtcaa gcctctggca aagaaggttg
     2641 acgcagagga atacttcccc aaggagaccg tcgaaaagat gggcaagctg ggcatgatgg
     2701 gcatctattt cccgactgag gtcggcggtg caggcggcga tgtgctgtcc tacgtcatgg
     2761 ccgtggagga actgagcaag gtttgcggca ccaccggtgt catcgtctct gctcacacca
     2821 gcctgtgcgc tgcccccatt tacgagaacg gcacccccga gcagaaggct aagtacctgc
     2881 cgaagctctg cagcggcgag tggctgggtg ctttcggcct gaccgagccg ggcgctggta
     2941 ccgatgcgca gggtcagcag accattgcca aggaagatgg cgattactgg gtgctgaacg
     3001 gctccaagat cttcatcact aacgcaggct ttgctgatgt cttcatcgtc atcgccgtta
     3061 ccgataacgt gctggataag cgcggccgtc ccaccaagct gtgctccgcc ttcatcgtgg
     3121 agcgcaccga cccgggcttc tctgttggta aggcagagga caagatgggt atccgcggtt
     3181 cttctacctg tgagctgatc tttgaggatt gccgtatccc gaaggatcgt atgctgggtg
     3241 tgcgcggcaa gggcttccag ctggcaatgg ctacgctgga cggcggccgt atcggcatcg
     3301 cttctcaggc tctgggcatt gccgagggcg ctctggagga aaccattgct tacgtcaagg
     3361 agcgcaagca gttcggccgt gccatcgctg ctttccagaa cacccagttc gagctggctg
     3421 agatgaaggc tcgtgttgag gctgcgaagt atctggttta tgctgccgct ctgaagaagc
     3481 agcaggctat ggatggcgca aaggttcgtt acagcgtcga ggctgcacag gctaagctga
     3541 tcgcggcccg caccgcaagc gatgtgaccc gccgctgcct gcagctgttc ggtggttacg
     3601 gctacacccg tgactacccc atcgagcgca tgatgcgtga tgccaagatc accgagatct
     3661 acgagggcac cagcgaagtg cagatgatgg tcatttccgg cgcgctgctg aagtaaggga
     3721 ggcaagagtt acaatgaaag caattgtttg tgtaaagcag gttcctgata cctccggcaa
     3781 ggtgtccgtc aagcccgatg gcactctgga tcgtgcatcc atggcaacca tcaccaaccc
     3841 cgatgacctg aacgctctgg aggccgccct gaaactgaag gacgccaccg gctgcgaggt
     3901 cgttgttgtc accatgggtc ccccgccggc agagggcatg ctgcgtgagc tgctggcccg
     3961 cggcgctgat aaggctgttc tggtctccgg ccgtgagttc ggcggttccg atacctttgc
     4021 aaccagccag atcctggccg ctgccgtcaa caagatcggc gttggccccg aggacgttgt
     4081 gttctgcggc cgtcaggcaa tcgatggcga taccgcacag gtcggccccc agatcgctga
     4141 aaagctgcat ctgccgcagg tcacctacgt tgccgacatc cagaaggatg gcaacacgct
     4201 gaccgtgaag cgtatgctgg aggacggcta catgatggtc aaggtgcaga ctccctgcct
     4261 gctgacctgc atcaaggagc tgaacgaccc ccgctacatg agcgtgaacg gcatctttac
     4321 ctgctacgac aagcccatgg aagtgttcga ctacaacgca ctgaaggacg atccgctgat
     4381 cgaggtggac accattggcc tgaagggttc tccgacgaac gtctttaagt ccttcactcc
     4441 tccgcagaag ggcgcaggca ccatgctgca gggcgacgat gttgctgccc agctggctgg
     4501 catcctggcc aagaagcacc tgatctgatg aaaggagcat aggattacaa tggctgattt
     4561 taacgaatac aaaggcgtat gggtcttttg cgagcagcgc cagggcgcac tgatgtccac
     4621 tgacttcgag ctggttagcg aggcccgtaa gctggccgac gagctgggct gcgaggtcac
     4681 cggcctgctg ctgggcgaca acgtggaggg cattgccaag gaactgggcg gctacggtgc
     4741 cgacaaggtt ctggtctgcg acagccccct gctgaaggat tacaccaccg acgcatacgc
     4801 taaggtcgtg tgcgatatgg tcaacgagta caagcctgag gtgctgctga tcggcgctac
     4861 caacattggc cgcgacctcg gcccccgctg cgcagcccgt ctgcacaccg gtctaaccgc
     4921 cgacgctacc catctggata tcgacactgc aaagtacatc gacttcctga aggagagctc
     4981 caccatcgac ctgtcca
//
