LOCUS       c00250_gnlPro..        14159 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GBABBFII_250.
ACCESSION   c00250_gnlPro..
VERSION     c00250_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-30 21:24:29
            Original ID  :: gnl|Prokka|GBABBFII_250
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14159
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14159
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[863:4159]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      864..4159
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..14159
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14159
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..739)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     61.4; E-value: 2e-19)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg250_1"
                     /sec_met_domain="LysR_substrate (E-value: 6.6e-21,
                     bitscore: 65.0, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 6.5e-20, bitscore: 61.4,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGQTMLKSTHEIYEILNQTTDEIQQTTSATVGKINIG
                     LLSSIEDSAFIAFIAHYYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDETIKN
                     WKPYASKKIIDEQLLFLHHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNLS"
     CDS             complement(864..1715)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 65.2;
                     E-value: 2.4e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.2e-26, bitscore:
                     82.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLV
                     QIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVA
                     SN"
     CDS             complement(1847..4159)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_3"
                     /sec_met_domain="Gly_radical (E-value: 8.2e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.6e-244, bitscore:
                     801.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VFVRTVTSVIHFRRQTTMKQLDETKVPNYWEGFNGGDWQEEINVR
                     DFIEHNLNQYDGDESFLAGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHG
                     PGYLNKDLEKIVGVQTDKPFKRAFMPFGGIRMAEDALESYGFKTDPQEHKIFNEYRKTH
                     NQGVFDAYTPDMRKARHYKIVTGLPDAYGRGRIVSDFPRIAVYGIDRLMAEKFKDYNLT
                     GDGEMTDDVIKLREEINEQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAV
                     KTQNGAAMSVGRIDTVIDAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNS
                     LFSGDPIWATLSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFK
                     RYATEVSIQSSTIQYENDDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVL
                     YAINGGKDEIQEAQVGPEYAPITSDYIDYKEFMNKFDKMMDWLADTYVNALNVIHYMHD
                     KYYYEAAQLALKDTQLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAVDFVADN
                     DDYPRYGNNDDRADDIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTT
                     PNGRQKGEPFSPGANPAYGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDD
                     ETRKDTLVHMVDGYMENSGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADL
                     TKEQQDDVIARTFFEEM"
     CDS             complement(4122..4349)
                     /locus_tag="ctg250_4"
                     /transl_table=11
                     /translation="LTPPSVLIQMVAFLIIIGQISCAQFNKMPKSWAKRSNEAHLKNNI
                     TPIIWAIKRLHRFGGVLYCVCKNCHICNPL"
     CDS             complement(4360..6501)
                     /locus_tag="ctg250_5"
                     /transl_table=11
                     /translation="MHGNNGSMDGDPPAAMRYTEARLSKISKEMLADIDKETVDMVLNF
                     DDTAQEPTVLPAGFPNLLVNGATGISAGYATEIPPHNLREVINAVLYLLKHPTADLDTL
                     MQYVKGPDFPTGGIIQGLDGIKQAYETGRGKIVVRSRTHIETIRGGREKIVVTEIPYDV
                     NKAQMIKKIDELRINKKVDGIAEVRDESDRFGLSVVIELKKEADAHGILNYLFKNTDLQ
                     IAYNFNMVAIADMQPKLLGLKAMLEAYVEHRRDVVTRRTRFDLNKAAARQHIVEGLIKM
                     LSILDQVIAAIRASKDKGDAKRNLVKKFDFSEAQAEAIVSLQLYRLTNTDITALQKEAA
                     QLAKAIAEYQDILARPAARDRVIEGELKRIAKEYGDDRRSSIQAKIETLEVATTVTVAD
                     ETVMVQVSRDGYVKRSSLRSYQAVDPADNGLKANDLAIFTGELSTLQHLYIVTNAGNVI
                     YRPVHEISDARWKDTGEHLSQTVGLGTDEKVLAVFAFDQLDGAGSFVLGSSDGYIKQTA
                     LSDLQPQRTYKSKPMMAMKLKDPAAIVTNAYFTTDQQQDVFVVSRHAYGLTFPLSDVST
                     VGARATGVKSMDLKPDDEVVNFILVKDPTTAKVGILTQRGAFKKMALSEVGEMSRARRG
                     LLVLRELKRDPHRIVAMMSVDDATRLNVLTDTGKVTTLNPAQHPAGDRYSNGSFVIDTD
                     VLGKPVFVQTKEEPADQVE"
     CDS             complement(6488..6802)
                     /locus_tag="ctg250_6"
                     /transl_table=11
                     /translation="LANQIQELTLEEVMGDRFGRYSKYIIQERALPDVRDGLKPVQRRI
                     LFAMNQDGNTYDKGFRKSAKSVGNVMGNYPPHGDSSXXXDGSAEPGLEVTGPTDSDARE
                     "
     CDS             complement(6809..8821)
                     /locus_tag="ctg250_7"
                     /transl_table=11
                     /translation="MDKATQAYNDSSIQVLHGLEAVRKRPGMYIGSTDGRGLHHLVYEI
                     VDNSVDEALAGFGDEINVTIHADNSVTVQDHGRGMPTGMHPSGKPTIEVILTVLHAGGK
                     FNQNSYKTSGGLHGVGSSVVNALSESLTVRVVRDKHAFEEHFINGGHPDGSLQKLGKVN
                     EPNGTTITFKPDPTIFQTTVFSYDTLAERLRESAFLLKNITINLTDERTGKHDSYHYPD
                     GLVSFVKYLNEGKDALSQVINFEGKKDGIVVDFAGQYNDGYSENLISFVNNVRTGDGGT
                     HEIGLRSGMTRAFNEFARKVGLLKERDKNLEGSDVREGFAGILSVRIPEEILQFEGQTK
                     GKLGTPQARTVVDGIVYDKLSTFLMENGEESQELVRKAVKARDAREAARKARDDSRTGR
                     KRKKDQGLLSGKLTPAQTKNPKRNELYLVEGDSAGGSAKQGRDRKFQAILPLRGKVLNT
                     QKAKLQDIVKNEEINTMIYTIGAGVGADFDLQQANYDKIIIMTDADTDGAHIQILLLTF
                     FYRYMRPLIDAGRIYIALPPLYRLQKGAGAKTKISYAWTDEELAKETKAMGKGYTLTRF
                     KGLGEMNADQLWETTMNPETRTLIRVRIDDAQLAERRVTTLMGDRVEPRREWIEHNVKF
                     DMDDDGSILETPTANHEKLQFGAKQIVKQLDKKGK"
     CDS             complement(8877..9014)
                     /locus_tag="ctg250_8"
                     /transl_table=11
                     /translation="LKRARSRSNLRVRTLAAMVKARPSRPRPLTLRLLTAPARALIFYF
                     "
     CDS             complement(9011..9256)
                     /locus_tag="ctg250_9"
                     /transl_table=11
                     /translation="MPKRDHQPSNLRVRTLAAMVKARPSRPRPLTLRLLTAPARALLKR
                     VHQPRNLHIRTLGTMVKAQPSHPRPLMLRFLIGLLY"
     CDS             9536..10243
                     /locus_tag="ctg250_10"
                     /transl_table=11
                     /translation="MYVRFILLTYHSKVKNAKIVSSLRGGAHLILVLCFILAYFIGAIP
                     FGVVIGKLFYHTDIRKGGSHNIGTTNAYRMLGPVGGSIVLVLDILKGTLAASLPILFGI
                     EHHWLVLIVGLAAVFGHTFSIYIRFKGGKAVATSAGILLAYNPPFFVIAFAIWFSLILL
                     TSMVSVASTLGMVLITAWSLVYHDWLLTTVACGLLVVFLIKHRANFKRIKAGDENMVPF
                     GLGQYLRQHRGRS"
     CDS             complement(10324..11202)
                     /locus_tag="ctg250_11"
                     /transl_table=11
                     /translation="MVTIANDFLTVKISRHGAELTSIKDNLTGRERLWQADPAVWGRHA
                     PVLFPIVGALADNQYHYAGKTYHMGQHGFARDRDFTVLSATPFKASFVLKDDDKTRAMY
                     PFAFRLIITFALENNNLHVTYHVDNPDKSEPLFFSIGGHPGFKVPMTPDTQFSDYYLNF
                     KPRKSLVQIPLVAGEGIDYTHRTLAATDANLALTHETFDHDALIFALLGKTTFNLVSDK
                     TKHGVSVTMTDTPYLGVWSPYPTTGDFVCIEPWWGIADTIGSTGDLTRKLGINRLEPGT
                     AFEKGYMISIF"
     CDS             complement(11238..12647)
                     /locus_tag="ctg250_12"
                     /transl_table=11
                     /translation="MAEVQTPKQIVEKLDQYVIGQDDAKRAVAVALYNRYRRMQLSPKM
                     QEEITPKNLLMIGPTGVGKTEIARRLAKIVEAPFVKVEATKFTEVGYVGRDVESMVRDL
                     AETAVAMEEEKQFSKVRIRAEKEANKRLVKILVPAIRKEKNTNPMAEMMSMLQGMQNGK
                     MPAGDDADAGGEVTDDIRNKRLSVAEQLEKGLLEDHEITVAVNDEKKGGGMGDMYGQMG
                     IDLSDTIGALMPKKKVERTMTVKEAREIFIKEESDKLVNHADIYHDAIQSAENNGIIFI
                     DEIDKIAAGNKHNSGEVSREGVQRDILPIVEGSQINTKYGPINTDHILFIGSGAFAEAK
                     PSDLIAELQGRFPIRVELKDLTEKDFVRILTEPDNALIKQYIALIGADGVKITFTKEAV
                     DRIAEIATQVNHDTDNIGARRLATILEKLLEDVLYEGPDMAMGAVTVTQQYVDDKIGHI
                     AADSNMTRYIL"
     CDS             complement(12691..13269)
                     /locus_tag="ctg250_13"
                     /transl_table=11
                     /translation="MLRFLSASARAHLKRSMTMTTIAAVRKDGVTALAGDGQVTLGEKV
                     IMKGNAQKVRRIYHDQVVIGFAGGVADAFTLQDWFEKKLEHYAGNLRRSAVALAQDWRK
                     DPTLQKLEAMMIVMDEHDLLLVSGSGEVIDPDEDVVAIGSGGNFAQAAAIAMLRHAPDM
                     TPADIAKEAVNIAGNIDIFTNHNVIVESF"
     CDS             13289..13426
                     /locus_tag="ctg250_14"
                     /transl_table=11
                     /translation="MAEFWLFRLPGHYVGFCDCERVICVSRRIKTAKVAINLLSPMFLA
                     "
ORIGIN
        1 ctgacaagtt aacgaattgg gttaactgat atggcgtggt gaaatgtgcg accacgtgcg
       61 ggaaatcaac catttgattc ttgtacgcct cacgcagaag ctgtccaagg aaatagtcgt
      121 caggataagt tgcccaaggt tcagctaaag tatctttgaa atggattcgc ttacggttag
      181 ccaaggcgtc atcgtggtgc aagaacagca attgttcatc aataattttc ttgctggcgt
      241 atggtttcca gttcttaatg gtctcatctg gcagattcat gatagccaaa tcaatctggt
      301 tgttttccaa ggattcccaa atttcgtgac gggtcatcat ccgtagcgtc acttcaactt
      361 caggattcag ttgatagtaa tgagcaatga acgcaataaa ggcgctgtcc tcaattgagc
      421 ttaataaccc aatattgatt tttccgacgg ttgcactggt tgtttgttga atttcgtcgg
      481 ttgtctggtt taagatctcg tagatctcat gggttgactt taacatggtc tgaccagcgt
      541 cagataaatg cagtttcttc ccgacagaat aaaacagcgg tgcccccacc gtccgttcca
      601 atttcttgat ctgctgtgtt aaggctggct gggtaatgcc aagcaattgg gcggcttggg
      661 tgtagttcat ggtttctgcc aattgcagaa aataagttag tgtctttgag gaaaaaatgc
      721 gctcttgttt cgttttcata gggcggcttc cctttcgttt ataatttaat aatatcgttc
      781 aaccatcatc ataagtggca atgaaaaaaa acgcaacgaa tttgccgcgt ttttaatcgt
      841 tacgttaagt tctagctaaa aattcagttg ctcgcaactg gtttcggacg ccagctttgc
      901 catccggtat aatccttaac gtgcagcaga ttctctgcat tcttaacgcg atcggcagac
      961 ggcggttcga tgccttcaag cgggtacgta atgccaagct cgtgatactt tttaacgcct
     1021 aacgtgtgat acggcagaat ctcaactttt tggaccacgt ttttgtccaa tgtcgcaata
     1081 tagtccccta accgtttgag ataagggtca aagtcagtgc gttccgggac cagaacgtgg
     1141 cgaatccaca tgtcatcacc atgcgccgac atatactgca ccatatccaa aatattttca
     1201 ttaggaaaac cggtgagctt tttatgctcg gccgaatcaa tgtgttttat atcaactaat
     1261 gagatgtcag taacagccat cagcttttca aactggccaa accacggttg gtcacgcgtg
     1321 aaggggccgc ctgacgtatc gaggcaagtc gaaattccca gtgccttagc cttggtgaag
     1381 agttcgagaa tgaaatcgat ttgtaccaaa ctctcaccgc cactgcaggt aataccccct
     1441 tggtcacccc agaaagcttt atagcgctgg gcatccgcca aaatttgatc agccgtcatt
     1501 tcttcaccaa ccccaatgtt ccaggtgtca gggttgtgac aatactggca acgcatgcga
     1561 cacccttgca taaagacaac gaagcgaata ccagggccat cgaccgaacc aaaactttcg
     1621 accgagtgaa catacccttt gatcgggcca tcatgatcct gaccaacttg ttctaagata
     1681 tttaatgggc tttgtgactg atcgatgagt ttcatcttat tgcctccact acaacatcat
     1741 gtgtcgtttt caattgattt tcatttcatt ctgccaaaaa acggcaacag catcgttgcc
     1801 gtcttttggc aaaacaggaa tgacttcctg tcttgagtta agctgcttac atttcttcga
     1861 agaaggtccg agcaatgaca tcgtcttgtt gttccttggt caaatctgcg aagtagacgc
     1921 agtagccaga aacccgaacg gtcaatgttg ggtattcttc tgggtgcttc tgagcatcaa
     1981 tcagggtctc cttgttgaag acgttgatgt tcaaatgcat gccgctgttt tccatatagc
     2041 cgtcaaccat gtgaaccaag gtatccttac gagtttcatc atcgtggccc agtgtgttcg
     2101 gagtaacccc aaacgtatta ctaatgccgt cagttgcgta atgatacgga atcttggcgg
     2161 tggacatcag ggatgccaat gcgccgttct tttctgcgcc gtatgccggg ttggcacctg
     2221 gtgagaatgg ttcgcctttt tgacgaccgt ttggtgttgt ccctgtattc ttaccataaa
     2281 ccacgttgga agtaatggtc aggacactgg tactcaactt cgcgccgcga tacagatgat
     2341 gcgtgttcat cttgttatag aaggtcttaa ccaaccactt agcgatgtca tcagcgcgat
     2401 catcgttgtt accatagcgt ggatagtcat cattgtcagc aacgaagtca actgcaacac
     2461 cgttttcatc gcgaattgcc ttgacatgac catacttgat tgcactgatg gaatcaactg
     2521 cgtgtgacag gccggaaatc ccggttgcga aggtccggtt cagttgggta tccttcaatg
     2581 ccagctgtgc agcttcataa taatacttgt catgcatata gtgaatcaca ttcaaagcat
     2641 tgacatacgt gtcggccagc cagtccatca tcttgtcgaa cttattcatg aattctttgt
     2701 aatcaatgta atcgctggtg atcggtgcat attcaggccc tacttgcgct tcctgaattt
     2761 catctttccc accgttgatc gcatacagaa ctgtcttagc taagttagca cgggcaccaa
     2821 agtattgaac gccatcagca attggttgtg ccgagacaca gcaagcaata ccgtagtaat
     2881 cggtacccca ttcgttgcgc atcagatcat cattttcata ttgaatggtt gaactttgga
     2941 tggatacttc ggttgcatag cgtttgaagc cttctggcaa gcgttcgctc caaagcaagg
     3001 tgatgtttgg ttctggtgct gcgcccatgt tgtccaaagt cttcaggaaa cggaaagccg
     3061 tcttggtaac gtggtgacgg ccatcattgc ccattcctgc caatgacaag gttgcccaga
     3121 ttggatcgcc tgagaacagt gagttatatt cttcagtccg gatgaaacga accatccgca
     3181 atttcataac caaatgatca atcagttcct gtgcctgttc ttcgttcaat ttgcctaagc
     3241 gcatatcgcg ttggatataa gcatcaataa ccgtatcgat ccggccaacg gacattgcgg
     3301 caccgttttg agtcttaaca gcagccagat agccaaagta gatccactga acagcttctt
     3361 gcgcattagc tgccggacgc gagatgtcat agccatattc cttggccatc ttcttcatgt
     3421 cgtttaaggc acgatattgt tcattgattt cttcacgcag tttgatcaca tcgtcagtca
     3481 tttcgccgtc gccagtcaag ttataatcct tgaacttctc ggccatcaaa cgatcgatcc
     3541 cgtaaacagc gatccgtggg aaatcagaaa caatccggcc acgaccatat gcgtctggca
     3601 agccagtcac gatcttgtaa tggcgagcct ttcgcatatc aggggtataa gcatcaaaga
     3661 caccttggtt atgggtctta cgatattcat tgaaaatctt gtgttcttga ggatctgtct
     3721 tgaagccata agattccaat gcatcttcag ccattcgaat accgccaaat ggcatgaatg
     3781 ctcgtttgaa tggcttgtcg gtctgaacac caacaatctt ttcaagatct ttgttcaaat
     3841 aaccagggcc gtgtgaggtg atcgtagatg gaatattgtt gtcagcatcc aaaacaccgc
     3901 catttgcacg ttcttgcttc tttaaattta gaacctgatt gttcaggact gttgtcgctt
     3961 cagtggggcc agcaaggaaa ctttcgtccc cgtcatattg gttcaggtta tgctcgataa
     4021 aatcgcgcac attaatttct tcttgccagt cgccgccgtt aaagccttcc caataattag
     4081 gaactttggt ttcgtcaagc tgtttcatag ttgtttgcct cctaaagtgg attacagatg
     4141 tgacagttct tacaaacaca gtataacacg ccgccaaatc tatgcaagcg ttttattgcc
     4201 catataatag gcgttatatt gttttttaag tgtgcttcgt tgcttctttt cgcccatgat
     4261 ttcggcattt tgttaaattg tgcacaactg atctgtccta taataatcaa aaacgccacc
     4321 atctgtatta gtacagacgg cggcgtcaat gatcaccatt tattcgactt gatctgctgg
     4381 ttcttcttta gtttgaacaa aaactggctt gccaaggaca tcagtgtcaa tgacaaacga
     4441 accattcgaa tatcggtcac ctgctggatg ttgtgccgga ttaagggtcg ttaccttacc
     4501 cgtatcggtc aaaacattca ggcgggtagc gtcatcgact gacatcatcg cgacaatccg
     4561 atgcggatca cgcttaagct cacgcaagac cagtaaacca cgacgggcac gactcatttc
     4621 cccgacctcg cttaaggcca tctttttgaa ggcgccacgt tgggtcaaaa taccgacctt
     4681 agccgtagtt ggatccttca ctagaatgaa attgacaacc tcatcgtctg gctttagatc
     4741 catactttta acgccggttg cccgggcacc gacagttgac acatcgctta acgggaatgt
     4801 cagtccgtaa gcgtggcggc tgaccacaaa gacatcctgt tgctgatcag tcgtgaagta
     4861 tgcattggtg acaatcgcgg ctgggtcttt cagcttcatc gccatcatcg gcttactctt
     4921 gtacgtccgt tgcggctgca aatcacttaa ggcagtttgt ttgatgtacc cgtcacttga
     4981 ccctaacaca aaactgccag cgccatcgag ttgatcaaaa gcaaacactg ccaagacttt
     5041 ttcgtcagtg ccaaggccaa ctgtttggct taagtgttcg ccggtgtctt tccaacgcgc
     5101 atcactaatt tcatgcactg gtcgataaat gacatttccg gcatttgtca cgatgtaaag
     5161 atgctgcaac gtactcagtt cgccagtgaa aatggcaaga tcattggcct tcaaaccatt
     5221 gtcggccgga tcaaccgcct gataactgcg cagtgaactg cgtttaacgt agccatcacg
     5281 agaaacctgc accatgacgg tttcatcagc caccgtcacc gttgtcgcca cttcaagcgt
     5341 ttcaattttg gcttgaatgc tgctgcgacg atcatccccg tactccttgg caatccgctt
     5401 caactcgcct tcaatcaccc gatcgcgggc agcggggcga gccaaaatat cttgatactc
     5461 ggcgattgct ttcgccaatt gtgctgcttc tttctgtaaa gccgtgatat cggtattggt
     5521 caatcgatac agctgcaagg aaacaatcgc ttccgcctga gcttccgaaa aatcaaactt
     5581 cttgactaag ttacgcttag catctccttt atccttggaa gcacgaatcg cggcgatgac
     5641 ctgatccaaa atcgatagca ttttgatcaa gccttcgaca atgtgctgtc gagcagcggc
     5701 cttgttgagg tcaaaacgcg ttcgccgagt gaccacatcg cgtcgatgtt cgacgtatgc
     5761 ttccaacatg gccttgagtc ctagtaactt cggctgcatg tcggcaatag cgaccatgtt
     5821 aaaattgtat gcaatttgca gatcggtatt tttaaataaa tagtttaaga tgccatgtgc
     5881 atcggcttct ttttttaatt cgatgacaac gctaagccca aaacggtcgc tttcgtcacg
     5941 gacttcggcg ataccgtcca cttttttgtt gatgcgtaat tcatcgattt ttttaatcat
     6001 ctgcgcttta ttgacatcat acgggatctc agtgaccacg atcttctcac gaccgccacg
     6061 aatggtttcg atgtgtgtcc gagaacgaac cacgatttta ccgcggccgg tttcgtaagc
     6121 ttgcttgata ccatcaagcc cttgaataat cccacctgtt ggaaaatcgg gacctttgac
     6181 gtattgcatt aaagtatcta aatcagcagt gggatgcttc agcaagtata agaccgcatt
     6241 gatcacttcg cgcaagttat gcggcggaat ttcggtcgcg taacctgcag agatgcccgt
     6301 tgcaccattc accagcaaat tcggaaatcc tgcgggcaac acagttggtt cctgtgccgt
     6361 gtcatcaaag ttcaacacca tatcgacggt ttccttatcg atatcggcca gcatttcctt
     6421 gctgattttg cttaaacgcg cttccgtata tcgcattgca gctggcggat caccatccat
     6481 ggaaccgtta ttcccgtgca tctgaatcag tggggcccgt aacttccaat cctggctcag
     6541 ccgaaccatc nnnnnnnnnn gagctgtcgc cgtggggagg ataattcccc atgacatttc
     6601 cgacactttt agccgacttg cggaagccct tatcatacgt attgccatct tggttcatcg
     6661 caaataaaat gcgacgctga accggtttga ggccgtcacg cacatcaggc agcgcccgtt
     6721 cttgaataat gtatttcgag taccggccaa accggtcgcc catgacctct tctaaggtca
     6781 gttcttgaat ctgattggcc aatggctttt actttccttt cttgtccagt tgtttgacga
     6841 tttgcttggc accaaactgt aatttttcgt gattagcagt cggggtttcc aaaatactgc
     6901 cgtcgtcatc catatcaaac ttgacgttat gctcaatcca ttcacgacgc ggctcaacgc
     6961 gatcacccat caaagtggtc acgcgtcgtt ccgccagctg agcatcgtca atccgaaccc
     7021 ggatcaaagt ccgcgtctca ggattcatag tcgtttccca aagctggtcg gcattcattt
     7081 ccccaagtcc tttaaatcgg gtcaatgtgt aacccttgcc catggccttg gtctctttgg
     7141 ccaactcttc atcggtccaa gcataactga tcttggtttt agcaccagcg cctttttgca
     7201 gccgatacaa tggtggcaaa gcaatgtaaa ttcggcctgc gtcaatcagc ggccgcatgt
     7261 agcgataaaa gaaggttagt agcaaaatct gaatatgcgc gccatcagta tccgcatcag
     7321 tcataataat gattttgtcg tagttggcct gctgcagatc aaaatccgca ccgacaccag
     7381 cgccgatggt gtaaatcatc gtattgattt cttcgttttt aacgatgtcc tgtaacttgg
     7441 ccttctgggt attcagtact ttccctcgca atggcaagat cgcctgaaac tttcggtctc
     7501 ggccttgttt agctgaacca ccggcagaat ccccttcgac gagatacaat tcgttacgct
     7561 tagggttctt ggtttgcgcc ggggtcaatt taccggaaag caaaccttgg tccttcttgc
     7621 gcttgcgtcc cgtccggcta tcgtcgcgcg cttttcgagc cgcttcccgc gcatcacggg
     7681 ctttgacggc tttacgaacc aactcttgcg actcttcacc gttttccatc aaaaaagttg
     7741 acagcttgtc atagacaatc ccatcgacca cagtgcgggc ttgcggcgta ccaagcttac
     7801 cctttgtctg gccctcaaat tgcaaaattt cttctggaat gcggacactt aaaatacctg
     7861 caaaaccttc acgcacatca ctgccttcaa ggttcttgtc gcgttccttc agcaagccga
     7921 cttttcgggc aaattcattg aatgcacggg tcatgccgct gcgcaagcca atttcatgcg
     7981 tcccgccatc accagtgcga acgttattaa caaacgaaat caggttttca gaatagccat
     8041 cgttatattg gccggcaaaa tcaaccacga taccgtcctt tttgccttca aaattgatca
     8101 cctgtgacaa tgcgtctttg ccttcattaa ggtatttaac aaaactgacc aagccatcag
     8161 gatagtggta actgtcatgc ttgccggtgc gttcatcagt cagattaatc gtaatatttt
     8221 tcagtagaaa ggctgattcg cgcaagcgtt cggccaaggt atcataactg aagacggtgg
     8281 tttgaaaaat agtcgggtcc ggcttgaacg tgatggtcgt gccattaggt tcattaactt
     8341 tgcccaactt ctgcaaactg ccatcaggat gaccgccatt gataaaatgc tcctcaaatg
     8401 cgtgcttatc ccgcacaact cgcacagtca atgactccga tagggcattg acgacgcttg
     8461 agccaacccc atgcaaacca ccagaagttt tgtaactatt ttggttaaac tttccgccgg
     8521 catgtagcac ggttaaaatg acttcaatcg tcggtttgcc actcgggtgc atccccgttg
     8581 gcatcccgcg accatgatcc tgtacggtca cagagttatc ggcgtgaatc gtcacattga
     8641 tctcatcccc aaaccccgcg agcgcttcat cgacggagtt gtccacgatt tcatagacca
     8701 agtgatgcaa accgcggcca tcggtggaac caatatacat ccccggacgc ttccgcacag
     8761 cttccaagcc atgcagcacc tgaatggatg aatcattata tgcttgcgtt gctttatcca
     8821 tagtaaactc ccttttttga acaactaagt tatagaatac accacgcgtt gagatttcag
     8881 aaataaaaaa taagagcgcg agcaggagcg gttagcaacc ggagtgtaag cggccttgga
     8941 cgtgatggtc gggctttgac cattgcggcc aaggtcctta cacgcaggtt gctgcgactg
     9001 cgagcgcgtt tcaatagagc agcccgatta gaaaccggag cataagcggc cttgggtgcg
     9061 atggttgggc tttgaccatt gtgcccaagg tccttatgtg caggtttctg ggctggtgaa
     9121 cgcgtttcag tagagcgcga gcaggagcgg ttagcaaccg gagtgtaagc ggccttggac
     9181 gtgatggtcg ggctttgacc attgcggcca aggtccttac acgcaggttg ctgggctggt
     9241 gatcgcgttt aggcatcaac accagacctc accgtattgc tctcaggact ccagttttct
     9301 cctcaaaatc gacccagctg gctaatacca tcattgttca tttaaatttt attagccgtg
     9361 tcaatgtccc aaagctcagc agaaaaccgc acttccctaa gcatggcaca taactatgac
     9421 ttcttgacgt gaaattcatc gcccgctggc accatatcta cgctgtgccc cgccatgatc
     9481 cttgcgcaag acaacaccaa atcgcgccac aacccgcttc acaggtttta ttcgaatgta
     9541 cgttcgtttt attctactga cgtatcactc aaaagtcaaa aatgctaaaa tagtttctag
     9601 tttgagagga ggggcacatt tgatacttgt gctttgtttt atactcgcgt atttcatagg
     9661 cgccatcccg tttggtgtgg tcattggtaa actcttttat cacaccgata tccgcaaagg
     9721 cgggagtcac aatattggca ccacaaatgc ttatcgcatg ttagggccag tcggcggctc
     9781 aattgtcttg gtgctggata tcttaaaagg cacgttggct gccagcttac ccattctctt
     9841 tggcatcgag catcattggc tggtgctgat cgtgggttta gcggccgtat tcggtcatac
     9901 attttcgatc tacattcgct ttaaaggcgg taaggcggtg gcaactagcg ctggcatttt
     9961 acttgcatat aatccgccat tttttgtcat tgcatttgcc atctggttta gtttgatctt
    10021 gctaacgtcc atggtcagcg ttgccagcac actgggtatg gtgttgatca cagcttggtc
    10081 gcttgtttac catgattggt tgctgacaac tgttgcctgc gggttgctgg ttgtcttttt
    10141 gatcaaacat cgggcaaact tcaaacgcat caaggccgga gatgaaaata tggtgccctt
    10201 tggcttaggc caatatcttc gtcaacaccg gggccgctct tagtcacctt ttgatggcaa
    10261 ccaaaaagac atccgaaact ggatgtcttt tttcatgtac ctaatcgaca aactgatgac
    10321 accttagaaa atgctgatca tgtaaccctt ctcaaatgcc gtgccaggtt caagtcgatt
    10381 gatcccgagt ttgcgcgtca ggtcgccagt gctgccaatc gtgtcggcga tgccccacca
    10441 aggttcgatg cagacaaagt cgcccgtggt tggatacgga ctccaaacgc ccagatatgg
    10501 ggtatcggtc atggtaacgc tgacaccatg tttcgtcttg tcagaaacca agttgaaggt
    10561 tgtcttacca agcaacgcga aaattaaagc atcatgatca aatgtttcgt gcgttaaggc
    10621 caaattggca tcagttgcag ccaaggtccg gtgcgtgtaa tcaatgcctt caccagcaac
    10681 caatggaatc tgaacaagcg acttacgcgg tttgaagttc aaataataat cggagaattg
    10741 agtatccggc gtcattggga ccttgaagcc cggatggccg ccgatgctaa agaacagtgg
    10801 ctcacttttg tccggattgt caacgtgata cgtgacatgt agattgttat tttctagtgc
    10861 aaacgtgatg attaaacgaa aagcgaacgg gtacatcgcc cgcgttttgt catcatcctt
    10921 cagaacgaag ctcgctttaa acggtgtcgc agagagcacc gtgaaatcac gatcacgggc
    10981 aaaaccgtgc tggcccatgt ggtaagtctt gcctgcgtaa tgatattgat tgtcagccag
    11041 cgccccaaca atcgggaaca agaccggggc gtggcgaccc cacaccgcag gatctgcttg
    11101 ccacagacgc tccctgcctg tcaggttatc tttgatactg gtcagttcag caccatgccg
    11161 actgatttta actgtcaaaa agtcatttgc aatcgttacc atacatcctc atcctttacc
    11221 tacaacatta ttgttaacta caaaatatac cgcgtcatgt ttgaatcagc cgcaatatgg
    11281 ccaatcttat catccacata ttgctgggtg acggtcacag cgcccatcgc catatctgga
    11341 ccttcataaa gtacgtcttc aagcagtttt tcaagaatgg tcgccagtcg ccgtgcccca
    11401 atattatcag tgtcgtggtt gacctgagtc gcaatctctg caattcgatc aaccgcttcc
    11461 ttcgtgaagg tgatcttcac gccatctgca ccaatcaagg cgatatactg cttgatcaaa
    11521 gcattgtctg gctcggtcaa gatccgcaca aaatcttttt cggttaagtc cttcaattca
    11581 acccgaattg gaaaacgccc ttgaagttca gcaatcaaat cactaggctt agcctcggca
    11641 aacgcacctg agccaatgaa caagatgtga tcagtattga taggaccata cttagtgtta
    11701 atctgcgatc cttctacaat cggtaaaata tctcgctgca caccttcacg cgaaacttcg
    11761 ccgctattat gcttgtttcc cgcagcaatt ttatcgattt cgtcgatgaa aatgatcccg
    11821 ttattctcgg cactttgaat ggcgtcgtga tagatgtcgg catgattgac cagtttgtca
    11881 gattcctctt tgatgaagat ttcgcgagct tctttgacgg tcatggtccg ctcaaccttt
    11941 ttcttaggca ttaaagcgcc aatggtgtcg cttagatcaa tgcccatctg accgtacatg
    12001 tcacccatgc caccgccttt tttctcatca ttgacagcaa cggtgatttc atgatcttca
    12061 agtaatcctt tttccagttg ctctgccacg cttaaacgct tattgcgaat atcatcggtc
    12121 acttcgccgc ctgcgtctgc atcatcgcca gctggcattt tgccattttg cattccttgc
    12181 aacatgctca tcatctcggc catcggattg gtattctttt ccttacgaat ggccggcact
    12241 aaaattttaa ccaaacgctt attggcttct ttttcggcac gtatgcgaac ctttgaaaat
    12301 tgcttctctt cttccatggc aacggcagtt tcggcaagat ctcggaccat ggattcgacg
    12361 tcacggccaa cgtagccaac ttccgtaaac ttagtcgctt ccaccttcac aaatggcgcc
    12421 tcaactattt tcgccagtcg tcgtgcaatt tcggttttcc cgacaccggt tggtccgatc
    12481 atgagtaagt ttttcggggt aatctcttct tgcatttttg gcgataattg cattctccga
    12541 taacggttat acaaggcaac cgcgacggcc cgttttgcat catcttgtcc aatgacatat
    12601 tgatcgagtt tttcaacgat ctgttttggg gtttgtactt ctgccatcat aagcctcctg
    12661 taggtcaatg taattgccga cggttacaaa ctaaaagctt tcgacaataa cattgtggtt
    12721 tgtgaaaata tcgatattcc ctgcgatatt cactgcttct ttggcaatgt cagctggtgt
    12781 catatctggg gcatgccgca gcatcgcgat tgccgcggct tgcgcaaagt tgccgccgga
    12841 gccgatcgct accacgtctt catctggatc tatcacttcg ccagacccgg atacgagtaa
    12901 caggtcatgc tcatccatca cgatcatcat ggcctcaagt ttctgcaagg tcggatcctt
    12961 gcgccaatct tgagccagcg caactgcgga acgtcgcaag ttgcccgcat aatgctccag
    13021 cttcttttca aaccagtctt gcagggtgaa agcatctgcc accccgccgg caaacccgat
    13081 gacgacttga tcatgataga tccggcgaac tttctgcgcg ttgcctttca tgatgacctt
    13141 ttcgccaagg gttacctgac cgtcaccggc caaagcggtc acaccatctt ttcttactgc
    13201 tgcaattgtt gtcatagtca tgctcctttt taagtgagcg cgagcagaag cgcttagaaa
    13261 ccggagcata agtgggctgg aagcctaaat ggctgaattt tggctattta ggcttccagg
    13321 tcattatgta ggtttctgcg actgcgagcg cgttatctgc gtatctcgtc ggataaaaac
    13381 agctaaagta gcgattaatc tgttatcgcc aatgttctta gcttaaaaaa ctcagactgc
    13441 ttttgcatca tgctttagtc ttaattatgc ttcggataat atttcatata ctcatttttt
    13501 aaatgtgcca tggtgacatg cgtgtagatt tgtgtggttg ataaacttgc atgacccagt
    13561 agctcttgaa cagttcgcag gtcagcacca tgatccagca tatgggttgc aaaactatgc
    13621 cgcagcatgt ggggatgaat gttggctgtc agcgtcgtct gcttgatgag ctgatccaaa
    13681 atatattcaa tgccacgcga ggtaatcgga cgcccatact ggttcacaaa tacatatcga
    13741 tgctgtgcct gacttttagc catcaagact tgtcggccat cttttaaata agttcgtagt
    13801 gcttgctgag cgtactgtcc aaagggcaca tacctatctt tgttgccttt accatgaatc
    13861 aacagtaatg ccgtattgaa atcgacctga ttcaatgtca gtttggcaca ctcggaaact
    13921 cgaataccgg tgccatacaa aacttccaac aaagcgcgat tacgttgatc cagcggggtt
    13981 ttgccagcaa ctgtcttgaa cagttcctga atctcggctt catagaaaaa ttgtggtaaa
    14041 tgatgatgct gcttttttaa ttcaaccagc tcaaatgggt tgcgtttgac gacatcaatt
    14101 cgtgtcaaat aacggtaaaa gcttcgcaaa ctactgattt ttcgggcaat cgatgtccg
//
