LOCUS       c00232_gnlPro..         5404 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AELLPBCO_232.
ACCESSION   c00232_gnlPro..
VERSION     c00232_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 20:38:34
            Original ID  :: gnl|Prokka|AELLPBCO_232
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5404
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5404
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2426:5403]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2427..5403
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5404
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5404
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             164..2011
                     /locus_tag="ctg232_1"
                     /transl_table=11
                     /translation="MECTQAEAFEQYIRDLRVVRSISRPSFPEGKAPAAVLEEIQTNAL
                     RCNTLMRQNEALLAQFVYDRDPASLTEADIQGLSAFAGRLFNYANSEDIGVAFKVHQLL
                     LAAARSRENVPMIVRELYYTGITLHYMNVRDEGTGINLLGDAIQVYFTEAAEYMSRYEQ
                     LDRNTREYLIRCVGNTRLGMSRGTHEESRRYLERFRRAMEVIRSAHYHALDPEFPWESY
                     IYSMHMDRMTLLIHLRQEEDPEVARQVLESAEYIWGHKKKHKGQDARLQNWRVPYFYAA
                     ARYHAGVGSLEDVVKILLELAGSVERDDYSAEAINRKLVLAAYLSVYAERLDDAGAQRY
                     RATVEQVRRSAYQYLEQMPASQYPRVVSSAAWELSKISSSSDETANRRMLGSILAGHKP
                     TYVHSLMVAELTRALLQRQIETRPETLVGLLGCRSAAEVQARREELCQTAYECGLYHDL
                     GKCAVLMYIDNNARRLLDEEFFCIQSHPRTGADILNRMGCGRTLALAALYHHCYYNGKG
                     GYPNDVPSCPPEIKGIVDALSVADSLDAATDNIGRCYNLAKPFRTLLEELRAQSGTRYA
                     PNVVALFEDERFCQQLAENTDAERKRVYLQVYHAGSEEK"
     CDS             complement(2102..2278)
                     /locus_tag="ctg232_2"
                     /transl_table=11
                     /translation="MAVYLDEACTRKAAEVTFPASTDWQEVQGSFSAPAGTFPLFFRLN
                     CTGRVDWESFILT"
     CDS             complement(2427..3182)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 68.7;
                     E-value: 2.1e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg232_3"
                     /sec_met_domain="Radical_SAM (E-value: 8.8e-30, bitscore:
                     92.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPHCDPVGLVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WSAEGGTEWSPEKLFQHVWRYRNYWGKKGGITVSGGEPLRQMDFLTKFFQLARAKGVHT
                     ALDTAGQPFQPDDPAYLAAFDTLMASTSLVILDLKEIDPEKHRQLTGRDNANILAMARH
                     ISDLGVPLWIRHVLVPGLTDDEEGLRRTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGI
                     SYPLPDAVPPTAEQVRRAEELLEVAKYPG"
     CDS             complement(3169..5403)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg232_4"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-42, bitscore:
                     132.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 7.1e-268, bitscore:
                     877.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="WEGFEGRIWKEEVNTRDFIQKNYTPYAGDESFLAGPTEATDKLWG
                     ALQRLQKEERAKGGVLDMETEVVSSLTAYGPGYIDESLKDLEQIVGLQTDKPLKRAFMP
                     YGGIKMAEQACTTYGYQPSAELHKIFTDYTRTHNQAVFDAYTPEMKKARHTHIITGLPD
                     TYGRGRIVGDYRRVALYGIDALIQFKQEDLANCGDGTMTDDVIRLREEIARQISALKGM
                     KKMAEAYGYDISQPAKDAKEACQWLYFGYLAAIKTQNGAAMSVGRISTFLDIYIQRDLD
                     RGILTESQAQELIDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVGGIGMDGRSMVTK
                     NCYRFLHTLENMGPAPEPNLTVLYSSALPEAFKKYAAKVSVNTSSVQYENDDVMKPVWG
                     DDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKSHEQCGPNYAPITSEYL
                     TYDEVLPKYVQMLDWLAGLYVNVLNLIQYMHDKYYYEEAEMALIDTDVRRTFATGIAGF
                     SHVIDSLSAIKYAKVKVVRDENGLATGFETEGDFPKYGNDDDRADEIGVWLLKTFLEMI
                     KKRHTYRNSEATTSILTITSNVVYGKYTGALPDGRAAFTPFAPGANPSYGAEQNGLLAS
                     LNSVAKLPYHWALDGISNTQTINPEALGHSEEERKENLVQVMDGYFDQGAHHLNVNVFG
                     KEKLLDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQMDVISRTCHAAL"
ORIGIN
        1 gtaaaagtgc attttataca aaaaatgacg tatgaatgcg taaaaatctt cggggggggg
       61 ggtaaaaatc tgcgaaaata agttgatttc tgccggacaa tgccgtatac tgaattatag
      121 tttcaaatcg aacgacaaac ttcggcaaaa aggggctgat aagatggagt gcacacaggc
      181 agaggccttt gaacagtata ttcgggatct gcgcgttgtg cgctcgatct cccggcccag
      241 ctttccggag ggcaaagctc ccgctgccgt gctggaagag atccagacca acgcgctgag
      301 atgcaacacc ctgatgcggc agaatgaggc actgctggcg cagtttgtct acgaccgcga
      361 ccctgcctcc ctgaccgaag cggatatcca gggactgagt gcctttgcgg gcaggctgtt
      421 caactacgcg aacagcgagg atataggtgt ggcattcaag gtgcatcagc tgctgctggc
      481 agcggcccgc agccgggaaa acgtgcccat gatcgtcagg gagctgtact acacgggcat
      541 caccctgcat tatatgaacg tccgggatga gggaactggc atcaacctgt tgggagatgc
      601 aatccaggtc tattttacgg aagctgctga atacatgtca cggtatgaac agctggacag
      661 gaatacccgg gagtatctca tccgctgtgt gggcaacacc cggctgggga tgtcccgcgg
      721 cacccacgag gaaagccgcc ggtatctgga gcggttccgc cgcgcaatgg aggtcatccg
      781 gtctgcgcat taccatgcac tggacccgga gttcccctgg gaaagctaca tctattccat
      841 gcacatggat cgcatgaccc tgctgatcca cctgcggcag gaggaagatc ctgaggtggc
      901 gcggcaggtg ctggaatcgg cggaatacat ctggggtcac aagaagaaac acaagggtca
      961 ggatgcacgc ctgcagaact ggcgggtgcc ctatttttac gccgctgccc gctaccatgc
     1021 cggggtgggc agcctggaag atgtggtcaa gatccttctg gaattggcgg gaagcgtgga
     1081 gcgggatgat tattctgccg aggccatcaa ccggaagctg gttctggcgg cctatctttc
     1141 ggtctatgca gaacgtctgg acgatgcggg agcacagagg tatcgtgcaa ccgtggagca
     1201 ggtacgtcgt tctgcgtatc agtatctgga acagatgccg gccagccagt atccccgtgt
     1261 ggtaagctct gccgcctggg agttgagcaa gatcagttcc agctcagatg aaacggcgaa
     1321 ccgccggatg ctgggcagta tcctggccgg gcacaagccg acctatgtcc actcgctgat
     1381 ggtggcagag ctgacccgtg ccctgctgca gcggcagatc gagacacggc ccgaaacact
     1441 ggtgggcctg ctgggctgca ggagcgcggc cgaggtgcag gcccggcggg aagagctctg
     1501 ccagacggcc tatgagtgcg gcctgtacca cgaccttggc aagtgtgccg ttctgatgta
     1561 catcgacaac aacgcccgcc gcctgctgga tgaggaattt ttctgcatcc agagccaccc
     1621 aaggaccggc gctgacattc tgaatagaat gggctgcgga agaacgctgg ccctggcggc
     1681 gctctatcac cactgctatt acaacggcaa aggcggctat cccaatgatg taccctcctg
     1741 cccgccggag atcaagggaa tcgtggatgc gctgagcgtg gcagactcgc tggatgccgc
     1801 caccgataat atcggtcggt gctacaacct tgccaagccc ttccgcacgc tgctggaaga
     1861 gctgcgggcc cagagcggca cccgctatgc gcccaatgtg gtcgccctgt tcgaggatga
     1921 gcgtttctgc cagcagctgg cagagaacac ggatgccgag cgcaaaaggg tgtatctgca
     1981 ggtgtatcac gccggaagtg aagaaaaata aacgaccctc tctatcattg cttcgcagtg
     2041 ccacctcccc aaaaagggag cgggcatacg tcagcgtgat agagagggtc attctttttg
     2101 tttaagtcag gatgaagctt tcccagtcca ccctgccggt gcagttcagc cggaagaaga
     2161 gcgggaaggt tcctgccggg gcagaaaagc tgccctgtac ctcctgccag tccgtgctgg
     2221 cggggaaggt gacctcagcg gctttccggg tgcaggcctc gtccagatag acggccatgc
     2281 aaaaagagcc ctccggcgct cacgcagttt accccggaaa agcagggggg tccctgatgc
     2341 gtgagccgcc gaagggctcc atcttctgtt tgattttatt aaatcagatg caaaaagctt
     2401 tgtcaagcac cggcggctgc atcgcttcag ccggggtact tagccacttc cagcagttcc
     2461 tccgcccggc ggacctgttc cgccgtgggg ggaacagcat cgggcagggg ataggagatg
     2521 cccagcttct gccacttgaa cagccccagc gtgtggtagg ggagaacctc cacccgctgc
     2581 accgttttca ggctgcggat gaagtctgcg gtcctgcgca ggccctcctc gtcatcggtg
     2641 aggccgggca ccagcacatg ccggatccag agcggaacgc ccagatcaga gatatgccgt
     2701 gccatggcta aaatgttggc attgtcccgg ccggtaagct gacggtgctt ctccgggtcg
     2761 atctccttga gatccaggat caccagactg gtggaagcca tcagggtatc gaaggcggcc
     2821 aggtaggcgg ggtcatcggg ctggaacggc tgaccggccg tgtccagcgc ggtgtgcacc
     2881 cccttggcgc gcgccagctg gaagaatttt gtcaagaagt ccatctgccg cagcggctcg
     2941 ccgccgctga cggtgatgcc gcctttcttg ccccagtaat tgcggtagcg ccagacgtgt
     3001 tggaacagct tctccggaga ccattccgtg ccgccctcag cgctccacgt ttcggggttg
     3061 tggcagtatt tgcagcgcag ggcacaaccc tgcagaaaga caacgaaccg gacacccggc
     3121 ccgtccaccg agccgaagct ttccagcgag tggacgagac cgacagggtc acagtgcggc
     3181 atggcaggta cgggagatca cgtccatctg ctgctcgcgg gtcaggtcga tgaacttgac
     3241 cgcatagccg gagacgcgga tggtgaagtt ggcgtattct tccttttcgg ggtgctccat
     3301 ggcatccagc agcttttcct tgccgaacac gttcacgttc aggtggtgtg caccctgatc
     3361 gaagtagccg tccatcacct gcaccaggtt ctccttgcgc tcctcctcgc tgtggcccag
     3421 agcctcgggg ttgatggtct gggtgttgga gatgccgtcc agagcccagt ggtagggcag
     3481 cttggcaaca gagttcaggg aagccagcag gccgttctgc tccgcaccgt agctggggtt
     3541 tgcaccgggt gcaaaggggg taaaggcggc acggccatcg ggcagagcac cggtgtactt
     3601 gccgtacacc acgttggagg tgatggtcag gatggaggtg gtagcctcgg agttgcggta
     3661 ggtgtggcgc ttcttgatca tctcaaggaa ggtcttgagc agccacacgc cgatctcgtc
     3721 ggcgcggtcg tcatcgttgc cgtacttggg gaagtcgccc tcggtctcaa agccggtggc
     3781 aaggccgttc tcgtcgcgca ccaccttcac cttggcgtac ttgatggcgc tcagggagtc
     3841 gatgacatgg gagaagcctg caatgccggt ggcaaaggtg cggcgcacat cggtgtcgat
     3901 gagtgccatc tcggcttcct cgtagtagta cttgtcgtgc atatactgga tgaggttcag
     3961 cacgttgacg tacagcccgg ccagccagtc cagcatctgc acatacttgg gcagcacctc
     4021 gtcataggtc aggtactcgc tggtgatggg agcatagttg gggccgcact gctcgtggct
     4081 cttctcatcc acgccgccgt tgatggcgta cagcaggcac ttggcaaggt tggcgcgtgc
     4141 gccgaagaac tgcatctcct tgccggtctg ggtagcagac acgcagcagc agatggagta
     4201 atcgtcgccc cagacgggct tcatcacatc atcgttctcg tactggacgc tgctggtgtt
     4261 gacggaaact ttggcggcgt acttcttaaa ggcctcgggc agggcagagg agtacagcac
     4321 ggtcaggttg ggctccggtg cggggcccat gttttccagc gtgtgcagga agcggtagca
     4381 gttcttggtg accatgctgc ggccgtccat gccgatgccg cccacctcca gggtcgccca
     4441 cacggggtcg ccggagaaca gctggttgta gctggggatg cgggcaaact tgaccatgcg
     4501 gaacttcatg accatgtggt cgatgagctc ctgtgcctga ctttcggtca ggatgcccct
     4561 gtccagatcg cgctggatat aaatgtcaag gaaggtggag atgcggccca cgctcatggc
     4621 ggcgccgttc tgggtcttga tggcggccag atagccgaag tacagccact ggcaggcctc
     4681 cttggcgtcc ttggcgggct gggagatgtc gtagccgtaa gcctcggcca tcttcttcat
     4741 gcccttcagg gcgctgatct ggcgggcgat ctcctcgcgc agacggatga catcgtcggt
     4801 catggtgccg tcgccgcagt tggcaagatc ctcctgcttg aactggatca gggcgtcaat
     4861 gccgtacagg gcaacgcggc ggtagtcgcc cacgatgcgg ccgcggccgt aggtgtctgg
     4921 cagaccggtg atgatgtggg tgtggcgtgc cttcttcatc tccggggtgt aggcatcgaa
     4981 cacagcctga ttgtgggtgc gggtgtagtc ggtaaagatc ttgtgcagct cggcagaggg
     5041 ctggtagccg taggtggtgc aggcctgttc tgccatcttg atgccgccgt agggcatgaa
     5101 ggcgcgcttg aggggcttgt cggtctgcag acccacgatc tgctccagat ccttcaggct
     5161 ttcgtcgata tagccggggc cgtaagcagt caggctgctg accacttcgg tctccatatc
     5221 cagcacgccg cccttggcgc gctcttcttt ctgcaggcgc tgcagcgcgc cccacagttt
     5281 atccgtcgct tcagtgggcc ctgcaaggaa actttcgtcc cccgcatagg gcgtatagtt
     5341 tttctggatg aagtcgcggg tgttgacttc ttccttccag atgcggcctt caaagccttc
     5401 ccac
//
