LOCUS       c00001_gnlPro..         4142 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KDLPBJCI_1.
ACCESSION   c00001_gnlPro..
VERSION     c00001_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:18:26
            Original ID  :: gnl|Prokka|KDLPBJCI_1
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4142
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4142
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[588:3773]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      589..3773
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4142
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4142
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..220
                     /locus_tag="ctg1_1"
                     /transl_table=11
                     /translation="ELDQNREDYAKEIRDMQQCIRRVTDKSVKIQAQEARNKQLMKEKF
                     STVQKQATDVRKNSRAITGYYNSMKEW"
     CDS             complement(247..456)
                     /locus_tag="ctg1_2"
                     /transl_table=11
                     /translation="MNAKNRLGFLSFAQTVRPLLYIYLMYNLRTRIASAIFHAACYVIL
                     HVVLHIIRQSYNKVIISHMIFFIK"
     CDS             589..2841
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_3"
                     /sec_met_domain="Gly_radical (E-value: 1.5e-40, bitscore:
                     126.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.6e-272, bitscore:
                     892.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLEMKEWDGFEGRLWKEEINVRQFIQDNYTPYDGNEDFLAEPTEA
                     TNQLWGALQKLQKEERAKGGVLDMETEVVTGITAYGPGYIDESLKDLETIVGLQTDKPL
                     KRAFMPYGGIKMAVQACETYGYQVSDQLKEIFTKYHKTHNTAVFDAYTPEMMKVRHNKI
                     LTGLPDTYGRGRIVGDYRRVALYGIDHLIEEKKKDKANCGCGEMTDNVIRLREEIAEQI
                     KCLEDMKKLAEIYGYDISRPATNAKEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIY
                     FERDLKKGIITEQEVQELVDHFTMKLRMVKFARIPSYNQLFSGDPVWATLDVAGTGVDG
                     RSMVTKSDFRFLHTLENMGPAPEPNLTVLYSSRLPEAFKDYAARISIDTSSIQYENDDA
                     MKPVWGDDYAICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPDYKP
                     ITSEYLDYDEVMEKYDKMMDWLVDIYVNTLNLIQYMHDKYYYEAAELSLMDTDLKRTFA
                     TGIAGFSHVIDSLSAIKYAKVKAIRDENGIAKDFEIEGDFPRYGNDDDRADDIGVWLLQ
                     TFMDKLKKHHTYRDSEPTTSILTITSNVVYGKATGAMPDGRKAGEPLSPGANPSYGAEK
                     SGLLASLNSLTKIPYEWALDGISNTQTINPGALGNEEGERIENLVNVMDGYFDQGAHHL
                     NVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTKEQQMDVIARTCHDHM"
     CDS             3039..3773
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 70.9;
                     E-value: 4.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-32, bitscore:
                     101.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGNIHSIETFGSADGPGVRYLIFLKGCNLRCKYCHNPDTWAKTE
                     GEMKSAEEILQQALRYKRYWGKKGGITVSGGEALLQIDFVTELFTLAKEKGVNTCLDTA
                     GNPFTREKPFYSKFEKLMEVTDLFMLDIKEMDEDEHKQLTGQTNKNILDMATCLSDHGK
                     AMWIRHVLVPGITDDDGQLHRLRAFIDTLKTVERVEILPYHTLGVFKWKELGIPYALED
                     VEPPTKEQVEHAREIIIPGYKS"
     CDS             3836..4039
                     /locus_tag="ctg1_5"
                     /transl_table=11
                     /translation="MGHMHKKIAVKSHQTKRVGHMCKKIAEISRQKKKEGHRSKEKLYM
                     SQQYRWSGHKDKEKQYMSQQHQ"
     CDS             4070..4141
                     /locus_tag="ctg1_6"
                     /transl_table=11
                     /translation="MSRQQNKRTIGSSKSCVDLIFHPP"
ORIGIN
        1 agaactggat cagaaccgtg aagattatgc aaaggaaatc cgtgacatgc agcagtgcat
       61 tcgccgagta acggacaaaa gtgtcaagat tcaggcgcag gaagcccgca ataagcagtt
      121 gatgaaggaa aaattttcca ctgtgcagaa acaggcaacg gatgtaagga aaaacagccg
      181 tgcgattacc ggatactata acagtatgaa agaatggtag ttatttcata aaatggaaca
      241 aaagtactac ttgataaaaa atatcatatg acttataatg actttgttat atgactgacg
      301 gataatgtgg agcaccacgt gaagtataac atagcacgcc gcatgaaaaa tcgctgacgc
      361 gatgcgggtg cgtaggttat acatcagata gatgtataac aagggtcgta ccgtctgggc
      421 aaaagataaa aaacccaggc ggttttttgc gttcatttat attttccggt ttttggtaaa
      481 tatattgtac gaaaataaag acatgcgtgg gtattgacaa aatgatgaac gttagtgtaa
      541 tctatcaatg attagagaat actaaccaat aagagaagga ggatcaccat gttagaaatg
      601 aaagagtggg atggattcga aggacgtctt tggaaagaag agattaatgt ccgccagttc
      661 attcaggata actacacacc atatgatggt aacgaggatt tccttgcaga acctacagag
      721 gctaccaatc aactttgggg agcactgcag aaactgcaga aggaagagcg cgcaaagggc
      781 ggagtcctcg atatggagac cgaggttgta accggtatta cagcttatgg tccgggatat
      841 attgatgaat ctctaaagga tttagagaca attgtaggtt tacagacaga caagccgtta
      901 aagcgtgcat tcatgcctta cggtggaatc aagatggctg ttcaggcatg tgagacatac
      961 ggatatcagg taagtgacca attaaaggag atctttacca agtatcacaa gacacacaac
     1021 acagccgtat tcgatgctta cacaccggag atgatgaaag tacgtcacaa taagatctta
     1081 acaggtctgc cggatacata cggacgcgga cgtattgttg gtgattaccg ccgtgttgct
     1141 ttatacggta tcgaccacct catcgaggag aagaagaaag ataaggcaaa ctgtggctgt
     1201 ggtgagatga cagacaatgt gatccgttta agagaagaga tcgctgagca gatcaagtgt
     1261 ttagaggata tgaagaagct tgcagagatc tacggatacg atatttccag acctgcaaca
     1321 aacgcaaagg aagccgtaca gtggttatac tttggatacc ttgcagcaat caagacacag
     1381 aacggtgcag caatgtccgt tggtcgtgtt tccaccttcc ttgatatcta cttcgagaga
     1441 gacttaaaga aaggcatcat cacagagcag gaagtacagg aattggttga ccactttacc
     1501 atgaagcttc gtatggtaaa attcgcacgt attccttctt acaaccagct gttctcaggt
     1561 gatccggtct gggcaacact tgacgttgcg ggtacaggcg tagacggacg ttccatggtt
     1621 acaaagtccg acttccgttt cctgcataca ttagagaaca tgggaccggc tccggagcca
     1681 aaccttaccg tattatattc ttccagactt ccggaagcat tcaaggatta tgcagcacgt
     1741 atctctattg atacaagttc catccagtat gagaacgatg acgctatgaa accggtatgg
     1801 ggcgatgatt atgccatctg ctgctgtgta tctgctacac agacaggtaa ggagatgcag
     1861 ttcttcggtg cccgtgcaaa ccttgcaaag tgtctcctct acgcaatcaa cggtggtgtg
     1921 gatgagaaga ccggtcagca ggtaggaccg gattacaagc caattacatc tgagtacctt
     1981 gattatgatg aagtcatgga gaagtacgat aagatgatgg actggttagt agatatttat
     2041 gtaaatacct taaacctgat ccagtatatg catgacaaat attactatga agcagcagaa
     2101 ctttctttga tggatactga tctgaagaga acgttcgcaa ccggtattgc aggtttctca
     2161 cacgtaatcg attccctttc cgcaatcaag tacgcaaagg ttaaagctat ccgtgacgag
     2221 aatggaattg caaaagactt cgagattgag ggagacttcc caagatacgg taacgatgat
     2281 gaccgtgctg atgatatcgg agtatggctg ttacagacat tcatggataa gttaaagaag
     2341 catcacactt accgtgattc tgagcctaca acttctatcc ttaccattac ttccaacgtt
     2401 gtatatggta aagctacagg agcaatgcca gacggacgta aggctggcga gccactttca
     2461 ccgggagcta acccaagcta cggcgcagag aagagcggac ttcttgcttc cctgaactct
     2521 ctgacaaaga tcccgtatga gtgggcactt gacggaattt ccaataccca gaccatcaac
     2581 cctggtgcac ttggaaatga agaaggcgag cgtattgaga accttgtaaa cgttatggac
     2641 ggatattttg accagggtgc gcatcacctc aatgttaacg tgtttggcaa ggagaagctt
     2701 atcgacgcta tggagcatcc ggagaaagaa gagtacgcaa acttcaccat ccgtgtatcc
     2761 ggttacgctg tgaagttcat tgatctgacc aaagagcagc agatggacgt tatcgcacgt
     2821 acctgccacg atcatatgta ataggatcta aggggtcacg agagttgcga agcaacggag
     2881 taattcgtag atcatatccg ttggaatgaa taagaaatca gggttaatac atgaatgaat
     2941 atttgtgtat tagccctgtt tttatgatat aatttgcaca tactaatgaa atgataattc
     3001 gatgctttta ttggtttggt taaaaacgag gtaaaacaat gctaggaaat atacattcaa
     3061 ttgagacatt cggttccgca gacggaccgg gcgtgcgtta tctgattttt ttgaagggct
     3121 gcaatctgcg ctgtaaatac tgccataatc ccgatacctg ggcaaaaacc gagggggaaa
     3181 tgaaaagcgc agaagaaatt ttacaacagg cgctgcggta taaaagatac tggggcaaaa
     3241 agggcggtat taccgtaagt ggcggagaag cactgttgca gattgatttt gtcacagaat
     3301 tgtttacgct tgccaaagaa aaaggcgtca atacctgtct ggatacagcg ggcaacccgt
     3361 ttacccgtga gaaaccattc tattctaagt ttgaaaaact gatggaagtg acggatctgt
     3421 ttatgctcga cataaaggaa atggatgagg acgagcataa acaactgaca ggccagacga
     3481 ataaaaatat tctggatatg gcaacctgtc tgtcggatca cggtaaagcg atgtggattc
     3541 ggcacgtact tgtgccgggc attaccgatg atgatgggca gttacacagg ctccgtgcat
     3601 ttattgatac gttaaaaacc gtggaacgtg tggaaatttt gccatatcac acgctggggg
     3661 tatttaaatg gaaagaactt gggattcctt atgcactgga agatgtggag ccgccgacca
     3721 aagagcaggt ggaacatgca cgggaaatca ttattcccgg ttataaatcc tgattgtatg
     3781 aacaggaggg acatatgcaa aaaaggcagc tgtaaaatcc catcaaacaa agagaatggg
     3841 acatatgcac aaaaagatag ctgtgaaatc ccatcaaaca aagagagtgg gacatatgtg
     3901 caaaaagata gctgaaatat cccgacagaa aaagaaagag ggacatagaa gcaaggaaaa
     3961 attgtatatg tcccaacagt atagatggtc gggacataaa gataaagaaa aacagtatat
     4021 gtcccaacag caccaataag aaggatataa agataaagaa aaattgtata tgtccagaca
     4081 gcagaataag agaaccatag ggagcagtaa aagctgcgta gatctcattt tccacccccc
     4141 cc
//
