LOCUS       c00016_gnlPro..         5014 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KIBFGNFI_16.
ACCESSION   c00016_gnlPro..
VERSION     c00016_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:27:00
            Original ID  :: gnl|Prokka|KIBFGNFI_16
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5014
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5014
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3538:5012]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3539..5012
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5014
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5014
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             344..1561
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:Iron-containing alcohol dehydrogenase (Score:
                     335.6; E-value: 1e-102)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg16_1"
                     /sec_met_domain="Fe-ADH (E-value: 6.5e-104, bitscore:
                     335.6, seeds: 219, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARFTLPRDLYHGKGALEALKSFTGKKAMICVGGGSMKRFGFLDR
                     AVEYLKEAGMEVELFEGIEPDPSVETVMRGAEAMLKFEPDWIIAMGGGSPIDAAKAMWI
                     KYEYPEITFEEMCKVFGIPPLRRKAHFCAISSTSGTATEVTAFSIITDYQKGIKYPIAD
                     FEITPDVAIVDPDLAETMPKKLVAHTGMDAMTHAVEAYVSTAHCDYTDPLAIFAIRMIQ
                     GDLVDSYNGDMSKRDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFADYGAHIIHGAA
                     NAMYLPKVIAFNAKDPEAKKRYGVIADEMKLGGANDDEKVKLLIEYLRGMNDALNIPHC
                     IKHYGPDSYPTEQGFVPENVFLERLPEIAKNAIADACTGSNPRQPSQEEMEKLLKCCYY
                     DTEVDF"
     CDS             complement(1721..3118)
                     /locus_tag="ctg16_2"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRMEDDLKEDPADFAVWKAAKPGEPAWESPYGMGRPGWHIECSAMSRTHLGK
                     TIDLHCGGQDLIFPHHENEIAQSECANGCEFARYWMHNGFINVDNQKMSKSLHNFFTVR
                     DVANLYGYEPIRYFMLTAGYRMPLNYTVDLIESCKNSLERLYTCRENLDFALSKTDFGT
                     DETLKEKAAEARTKFCTAMDDDLNTPDALAAVFDLVKEINTLSAASSKAALETAAAAFD
                     EITGVLGLLYNRKKDEVPAEVTALVEKRAAAKKAKDWATADAIRAQLTELGWAVKDTAQ
                     GPQLSKL"
     CDS             complement(3539..4291)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.2;
                     E-value: 2.1e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg16_3"
                     /sec_met_domain="Radical_SAM (E-value: 3.3e-30, bitscore:
                     93.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKTLGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQMDFLTEFFTLARAKGVHTA
                     LDTAGQPFRPDDPVYLAAFDRLMANTSLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWIRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEDLLEVSRYPG"
     CDS             complement(4278..5012)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg16_4"
                     /sec_met_domain="Gly_radical (E-value: 1.5e-42, bitscore:
                     132.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.1e-41, bitscore:
                     129.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LIDTDVRRTFATGIAGFSHVIDSLSAIKYAKVKVVRDESGLATGF
                     ETEGDFPKYGNDDDRADEIGVWLLKTFLEMIKKRHTYRNSEATTSILTITSNVVYGKYT
                     GALPDGRAAFTPFAPGATPSYGAEQNGLLASLNSVAKLPYHWALDGISNTQTINPEALG
                     HSEGERVENLVQVLDGYFDQGAHHLNVNVFGKEKLLDAMEHPEKEEYANFTIRVSGYAV
                     KFIDLTREQQLDVLARTCHGVL"
ORIGIN
        1 cacgctgggg gtcgtcggtt cgagcccgat ccgggtcgcc atttgctgct atagctcagt
       61 tggtagagcg catccttggt aaggatgagg tctccagttc gaatctggat agcagctcca
      121 aaggcaagca cctcgaagtc ttaggacttc ggggtgtttt tttgcgcttt ttcagggcag
      181 ggggcttgtt gcatcctact gcttgtggaa accgcccaaa atagaacgac tttttcttcc
      241 gctacgtcaa ttgaagctgc ctccaaattg ttatataatt aacataataa tgaactgcgg
      301 tacagcgctg cgtgtgccgt actatttttg gaggttttgt attatggctc gttttactct
      361 gccccgtgat ctgtaccatg gcaagggcgc tctggaagcg ctgaagtcct tcaccggaaa
      421 aaaggcaatg atctgtgtcg gcggcggctc catgaagcgt ttcggcttcc tggatcgtgc
      481 cgtggagtac ctgaaggaag ccggcatgga agtggagctg ttcgagggca tcgaacccga
      541 tccttccgtg gagaccgtca tgcgcggtgc cgaggccatg ctgaagtttg agcccgactg
      601 gatcattgcc atgggcggcg gctctcccat cgatgcagcc aaggccatgt ggatcaagta
      661 cgagtacccc gagatcacct ttgaagagat gtgcaaggtg ttcggcatcc cgccgctgcg
      721 ccgcaaggca cacttctgtg ctatttcctc cacctctggc actgccaccg aggtgaccgc
      781 tttctccatt atcaccgact accagaaggg catcaagtac cccatcgcag actttgagat
      841 caccccggac gtggccatcg tggaccccga tctggctgag accatgccca aaaagctggt
      901 tgcccacacc ggtatggacg ccatgactca cgctgtggag gcttatgttt ccaccgcaca
      961 ctgcgactac accgacccgc tggccatctt cgccatccgc atgatccagg gcgatctggt
     1021 ggacagctac aatggcgata tgtccaagcg cgactccatg cacaacgccc agtgccttgc
     1081 cggtatggct ttctccaacg cactgttggg catcgtgcac tccatggcac acaagaccgg
     1141 cgctgccttt gcagactacg gcgcacacat catccacggt gcggccaacg ccatgtacct
     1201 gcccaaggtc atcgccttca acgccaagga ccccgaggcc aagaagcgct acggcgtcat
     1261 cgcagacgag atgaagctgg gcggcgcaaa cgatgacgaa aaggtcaagc tgcttatcga
     1321 gtacctgcgc ggcatgaacg acgcgctgaa catcccccac tgcatcaagc actacggccc
     1381 ggactcctac cccaccgagc agggctttgt gccggagaat gtgttcctgg agcgtctgcc
     1441 cgagatcgcc aagaacgcca tcgccgacgc ctgcaccggc tccaaccccc gccagcccag
     1501 tcaggaggag atggagaagc tgctcaagtg ctgctactac gatactgagg tggatttctg
     1561 atccgtcctt tctttattac gcctccttcc ctatggacaa aagccccgca gagcataccg
     1621 ccctgcgggg cttttgccgg tttcgcctgc attctcccgt gaaaaaaggg gttcggaaac
     1681 gcctacggcg tttccgaacc cctttcatac tactaaggcg ttacagcttg ctcagctggg
     1741 gaccctgtgc ggtgtccttg acagcccagc ccagttcggt cagctgtgcg cggatggcat
     1801 cagcggtggc ccagtccttg gccttcttgg cagcagcacg cttctccacc agcgcggtca
     1861 cctcggcggg cacttcgtcc ttcttgcggt tgtacagcag acccagcacg ccggtgatct
     1921 cgtcaaaggc ggcggcagcg gtctccagtg cagccttgct ggaagcagca gacagggtgt
     1981 tgatctcctt gaccagatca aacacggcag caagcgcatc cggggtgttc aggtcgtcgt
     2041 ccatggcggt gcagaacttg gtgcgtgcct cggcggcctt ttccttcaaa gtctcgtcgg
     2101 tgccaaagtc ggtcttgctc agggcaaagt ccagattctc gcggcaggtg tacaggcgct
     2161 ccagactgtt cttgcagctc tcgatgaggt ctacggtgta gttcaggggc atgcggtagc
     2221 cggcggtcag catgaagtag cggatgggct cgtagccgta gaggtttgcc acatcccgca
     2281 cggtaaagaa gttgtgcagg ctcttggaca tcttctgatt gtccacgttg atgaaaccgt
     2341 tgtgcatcca gtagcgggca aactcgcagc cgttggcgca ctcgctttgg gcgatctcgt
     2401 tttcgtggtg agggaagatc agatcctgac cgccgcagtg caggtcgatg gtcttgccca
     2461 gatgggtgcg gctcatggcg ctgcactcga tgtgccagcc cgggcggccc atgccgtagg
     2521 ggctttccca tgcgggctca ccgggctttg cagccttcca gacggcaaag tcggcgggat
     2581 cttccttcag atcgtcctcc atgcggctgc gcagctcgcg gttgccgctt tccagatcgt
     2641 ccagattcag gtggctcagc ttgccgtagc cggggtcgct cttcacgcgg aagtacacat
     2701 cgccgttctt ggcaacgtag gcgtggccgg agtcgatcag atccttgacg atatccagga
     2761 tctgagggat atgctcggtg gcgcggggct gtacggtggg cttttcgcag ttcagcccgg
     2821 cggcatcctt caggtactcg gcctcaaagc gctgtgccac ctccttcatg gaggtgccct
     2881 cttcctgtcc cttcttgatg agcttatcgt cgatatcggt gatgttggaa acgtagatca
     2941 ccttgttgcc ccggtattcc agatagcggc gcagggtgtc gaacacgatc agcgggcgtg
     3001 cgttgccgat gtggatatag ttgtacacgg tagggccgca gacgtagatg cggtactcgc
     3061 cgggcacctg cggcacaaat tcttccttct gtcgggtcag ggtgttgaag atctgcatgg
     3121 tcaattctcc tttttgttcc aaacagaagc cgcaaaacaa aacgcccccg gagcagtgca
     3181 cgcagcacag ctccgaaggc ggttttaaaa tcaaaatcgc ggttccactt ctgtttgtcg
     3241 ctcaaagccg gtaacggcgg cgcaccgcac ggcgatactt ccccgcaggg gttcccgccg
     3301 cgtgctgtcc gggcgcactt tgcgcagcgg tctgcaaaca ggcttccagc cagtggcctg
     3361 ttctctctgc gcagacggtg tgcggcgcta ctctgcccgg ataaacgcat ttatcctttt
     3421 acccctttat tatagcggtg cggtgcaggg aagtcaaggc agaacgaacc agtgaaaatg
     3481 aaattcagcc aagcctaccg gcttggctga atttcaaatc aaaaagatgc tttcctcttt
     3541 acccgggata ccggctcact tccagcagat cctctgcgcg ctttacctgc tctgccgtgg
     3601 gcggtacagc gtccggcagg ggatagggga tgcccagctt ttgccacttg aacagcccca
     3661 aggtgtggta gggcagcacc tccacccgct gcaccgtttt gagcgagcgg ataaagtctg
     3721 ctgttttgcg cagcccttcc tcgtcatcgg tcagaccggg cacgaggacg tgccggatcc
     3781 acagcgggat gccgaggtcg gagatgtgcc gcgccatggc aaggatgttg gcattgtctt
     3841 tgccggtgag ctgccggtgg cgctccgggt cgatctcttt gaggtctaag atcaccagac
     3901 tggtgttagc catcagacgg tcaaatgcgg ccagatagac cgggtcatca gggcggaagg
     3961 gctgcccggc ggtgtccagc gccgtgtgca cgcccttggc gcgcgccagc gtgaagaact
     4021 cggtcaggaa gtccatctgc cgcagcggct cgccgccgct gaccgtgatg ccgccctttt
     4081 tgccccagta gttgcggtag cggtataccc gctggaacag cgcttctgcc gtccacgcct
     4141 cgccgccctc tgcccatgtt tcggggttgt ggcagtactt gcaccgcagg gcacacccct
     4201 gcaaaaacac cacaaagcgc acgccggggc cgtccaccga gccgaaggat tccagcgagt
     4261 ggacgtaccc aagggtctta caggacgcca tggcaggtgc gtgccagcac gtccagctgc
     4321 tgctcgcggg tcaggtcgat gaacttgaca gcgtagccgg aaacgcggat ggtgaagttg
     4381 gcgtactctt ccttctcggg gtgctccatg gcatccagca gcttctcctt gccaaagacg
     4441 ttcacgttca ggtggtgtgc tccctgatcg aagtagccat ccagtacctg caccagattc
     4501 tccacgcgct cgccctcgct gtgacccaga gcctcggggt tgatggtctg ggtgttggag
     4561 atgccgtcca gtgcccagtg gtagggcagc tttgccacgg agttcagcga ggccagcaga
     4621 ccgttctgct ctgcgccgta gctgggggta gcgccgggtg caaacggggt gaaggcggcg
     4681 cggccatccg gcagagcgcc ggtgtatttg ccgtacacca cgttggaggt gatggtcagg
     4741 atggaggtgg tagcctcgga gttgcggtag gtgtggcgct tcttgatcat ctcaaggaag
     4801 gtcttgagca gccacacgcc gatctcgtcg gcgcggtcgt catcgttgcc gtacttgggg
     4861 aagtcgccct cggtctcaaa gccggtggca aggccgctct cgtcacgcac taccttcacc
     4921 ttggcgtact tgatggcgct cagggagtcg atgacgtggg agaagcctgc aatgccggtg
     4981 gcaaaggtgc ggcgcacgtc ggtgtcgata aggg
//
