LOCUS       c00313_gnlPro..         6083 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KJOMAKEE_313.
ACCESSION   c00313_gnlPro..
VERSION     c00313_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:01:30
            Original ID  :: gnl|Prokka|KJOMAKEE_313
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6083
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6083
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2296:5272]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2297..5272
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6083
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6083
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(132..1412)
                     /locus_tag="ctg313_1"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGEN
                     EALELRDGDKKRYGGKGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSK
                     LGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQE
                     FMIRPVGAASFKEGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSII
                     AAINAAGYEPGKDVMIGMDCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINE
                     YPIDSIEDGMSENDWAGWKKLTDRIGNHCQLVGDDLFVTNVEFLSRGIKEGCGNSILIK
                     VNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSD
                     RMAKYNQLLRIEEELGDRAVYGYKRIN"
     CDS             complement(1520..2098)
                     /locus_tag="ctg313_2"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             complement(2297..3031)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg313_3"
                     /sec_met_domain="Radical_SAM (E-value: 6.4e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(3032..5272)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg313_4"
                     /sec_met_domain="Gly_radical (E-value: 2.7e-46, bitscore:
                     145.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.5e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             5490..6038
                     /locus_tag="ctg313_5"
                     /transl_table=11
                     /translation="MRNILFTVLFIGILSLIASPISLASKSLHKANMINLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKKRKVTESYPGYMESDLVATDSSGITLAICKENRISCYTPFSDGFRDLPREGI
                     GPQTVRTLFAGTDGYFYLP"
ORIGIN
        1 cgatatattt tatgaggtta tagataacac tttatcaatc aataaaaagg gcctatcctc
       61 acggataagc ccttcaatat cttgggtttt ggattactac ataattagcg attataattt
      121 actttatata cttaattgat tcttttgtat ccgtatacgg cacggtctcc caattcctcc
      181 tcgatacgaa gcagctggtt atatttagcc atacggtccg aacgacttaa tgatcccgtc
      241 ttgatctgtc cgctattggt agcgacagcg atatcggcga tcgtagcgtc ttccgtctca
      301 ccggaacggt gtgaagtaac ggtcgtgtat ccatgacggt gagccatctc gatagcgttc
      361 agtgtctccg tcaaggaacc gatctgattt accttgatca agattgagtt accgcaacct
      421 tctttaatac cccttgaaag gaactctaca tttgtcacga acaaatcatc tccgactaat
      481 tggcaatggt taccaatacg atctgtcagt ttcttccaac cagcccaatc attctcactc
      541 ataccatcct caatggaatc gatcgggtat tcgttgatca gcttttccag ataatccact
      601 tgttcgtcgg aagtacgttt cacgcctttt tctccttcaa acttcgtgta atcgtagatt
      661 ccatccttat aaaactcaga agaagcgcaa tccataccga tcatcacatc cttgcccggt
      721 tcataaccag ccgcattgat agcggcgata attgagttca aggcatcttc cgtaccttcc
      781 aaattcgggg cgaaaccacc ctcatcacca acagcggtac ttaacccacg atcgtgcaat
      841 acctttttca acgcatggaa tacctcagcg cccatacgta agccttcctt gaaagaagcg
      901 gcacctaccg gacgaatcat aaattcttgg aaagcgatcg gagcgtcgct atgcgaaccg
      961 ccattgatta tattcatcat cggaacaggc atcacgtagg tattcgttcc accaatatat
     1021 ctatataaag gaatatccaa ataatttgcg gcagctttcg ctacagctaa agaaacgcca
     1081 aggatagcgt tcgcacctag tttcgattta gttttcgtac cgtctaaagc caacatcgca
     1141 tgatcgatac ctatctgatc caaggcagac atccccttca atgccggagc gataatcgta
     1201 ttgatattct caaccgcttt caatacgcct ttaccaccat aacgcttctt gtcgccatca
     1261 cgcaactcca aagcctcgtt ctcgccggtg gacgcaccgg aaggcacaga cgcacggccc
     1321 ataacgccgg actctagtaa aatatcaacc tctactgtcg ggttacctct tgaatctagg
     1381 atctcacgtc ctgtaatctt ttctattttc attgcttcta cgttttttaa actgacgcaa
     1441 atatagatgg attacttgaa ccgtgaaatc accacaaatt gctattttca cctcaataat
     1501 acctgaatgt aggtagagac tacaacactc ccgcccgcac ccggctcaaa gattcgggtg
     1561 tcatcaataa atacgaggcg atatgattga cagaagcacg tttcgctgct tccggatatt
     1621 cctgttgaaa acgcagataa cgttttcttg ccgactcaaa acgccaagag tccgctttct
     1681 cttgcgaaag catcaaagcc cactccagta ttttctggta caatctatta atagctaaag
     1741 atgttttcaa taaatcgcta aattccgaaa aagagatccg gtgataaacc gtcggttcca
     1801 atgcctcgat taataaacgc gtcggttctc tttggaatgt gctggctata caaataacga
     1861 tatttccctc acaggagaaa tgctcggtaa tatcacgttt attcttatag taaaactggc
     1921 gtaataatcc cgattccaca taatataaat atttagatat ttgtccgtta tccaacaata
     1981 attctccttt cgcagcctta cttgattcta tgatcgaggc cagagaatcc aaatccgcat
     2041 cgggtaatgg ataaaaacgt ttggatatca tttcggctat ttcttgtggt gtcttcatac
     2101 tattcatcat tttctgatct atatcatttt ttatctcctt acaaaaatag atatttgttg
     2161 catataacaa aacaaacaca tattaaatag tcaaggttag tctaggcaaa aaattattac
     2221 tatcgtttaa aagctaatcg gattagattg ttttccctga ttagctgttg cataatcgca
     2281 aggcttgatc gagagatcag gccttgttat tttctggttt gtagcgagag aatatcgctt
     2341 tcgcattatc taaacgttct tttgataagg gttccacccc ctttaacgga taatcgaggc
     2401 ctaattgctc atatttatag gttcccatcg tatgataagg caataattcc actttgcgaa
     2461 ccactttata tgaactgaca taccgagcta acgcctccag ccactcgtca ttatccgtat
     2521 atccggggac aataacatgg cgaatccaag tatctatgcc acgtctctcc aattcatcca
     2581 agaacagaag cgtattatcc tgcttgacac ccgccagtaa cggatgtaaa tccgggttta
     2641 aggttttgat gtccaataac actaagtccg cataatccaa gacttcccaa gcttttgagg
     2701 tacatacgaa accagatgtg tccaaagctg tgtgaagacc ttgctcttgg cacaaacgga
     2761 aaaactcttt cagaaactca ggttgcaata aaggttcccc accggtaaca gtcacccccc
     2821 cacgagcgat aaaactctta taccgtaaaa cttccgtcaa tagctcgccg ggagtcatct
     2881 gatatttcac cttccctttc gggttccaag tatccgggtt atggcaatac aggcaacgca
     2941 acgggcagcc ctgcataaaa acgacaaagc gaattccggg accatctacg gtcccgaaac
     3001 tctctaatga atgtatctta ccttttatca cttacagtga atggttaatg gttcttgaaa
     3061 taacatccaa ctgttgttca cgagtcagtt tcacgaagtt taccgcatat ccggatacac
     3121 ggatcgtaag ttgcggatat ttctcgggat gttccatagc gtcgatcaac aaatccttat
     3181 caaatacgtt aacattcaga tgctgacctc catccggagt aaaatagcca tccaataagc
     3241 taaccagatt attgatctgc atttcacgct cctttcccaa cgtagccgga gaaacagcga
     3301 aagtataaga gatgccgtcg tgcgcatgct ggaatggaag cttcgctacc gaagctagcg
     3361 cagcgatagc acctttcgta tcacgaccgt tcatagggtt cgcacccgga gcgaaaggag
     3421 ttccaccgat acggccatcc ggtgtcgtac cagtcttctt accatatacc acattagagg
     3481 tgatcgtaag gatggactgc gtaggtttcg catggcgata ggtctcgtgc tgacgcaaat
     3541 attccatgaa cttctcggtg atctctacag cgatactatc cgtacgatca tcgttattac
     3601 cgaaaggaac atattcgccc tcacgttcga aatcaaccgc caagccacgc tcgtcacgga
     3661 tcacacgtac tttcgtatca cggatagcag ccaacgaatc ggcaacgata gaaagaccgg
     3721 cgatacctgt cgcacggata cgctctacat ccccatcgtg aagcgacatc tcgaatgcct
     3781 catacgcgta tttatcatgc atataatgga ttatcttcaa ggcgttcaca tacactttcg
     3841 ccaaccagcg catcatttgc tcatacttag ccatcacctc atcgtattcc agatactcgc
     3901 cgcgaaccgg ctcgaacatc ggggctacct gtacgccgct gcgctcatcc cgtcctccgt
     3961 tgatcgcata taataaacat ttcgccaaat tcgcacgggc tccgaagaac tgcatctgct
     4021 taccgatctt cataggagaa acgcaacaag cgataccata atcatctcca taatccggac
     4081 gcatcaaatc gtcgttctca tattggatag ccgacgtatc gatagagacc ttggcacaga
     4141 aacgtttcca gttctcggga gcgttcttaa accacagaac cgtcaaattg ggttctggtg
     4201 ccggacctag attatataat gtatggagat aacgatagga agttcttgtt accaaagaac
     4261 ggccatccac tccttggccg cccaaggact ctgttaccca aaccggatca ccggagaaca
     4321 aatcattgta ttccggcgta cgtaagaaac gaacgatacg caacttaatg atcagctggt
     4381 ctaccaattc ttgcgcctcc tcttcggtca acttaccctc acggatgtct ttctcgatat
     4441 aaatatccaa aaacgtagaa gtacgtccga tagacatcgc cgcgccatct tggtctttta
     4501 ccgccgccag ataagctaag tacacaaact gaactgcctc acgagcgttt tgtgccggac
     4561 gagtcacatc aaagccatag ctagcgcaca ttttctcgaa cgctttcaaa gccttgatct
     4621 gttcagaaat ctcctcacgg ctctggatga tctcttccgt gatctcttgg atatccaaac
     4681 gattcaagaa acgctttttc tcctcgatca agattgccgt accatacaga gcgacacgac
     4741 gatagtcacc gataatacgt cctctgccat acgcatcggg cagaccggtg ataatagccg
     4801 aacgacgtgc ggcaatcatc tcttccgtat atgcggagaa aacgccctcg ttatgagtct
     4861 tacgatactt cgtaaagatc tctttcgtca tcgggtccaa atgataacca taagcttcca
     4921 agctattctc gaccatacgg ataccgcctt tcgggaagat accacgtttc agcggagcat
     4981 cggtttgtaa cccaacgatc acctcattct ccttatcaat atagcccggg ccatatacat
     5041 cgatcccttg aggtaatttt gtctcggcgt cataaactcc cttttccctc tcgaccttaa
     5101 acatctcggt cagcttgttc cagaccttac gagtcttctc agaagaagcg actaaaaagg
     5161 aatcatcccc ttcataaggc gtatagttac tctggataaa atctcttaca ttgatctctc
     5221 ttttccaggt ctcaccctta aagtgatccc agcactcatt ctcaaatttc ataaacaatc
     5281 ttatctaata ccacattttt accggcacaa aggtaaaact tttatcctca tgttcctgta
     5341 acatttgcga cacccctcta aaatccctat aaataggtat taaaaatata caagaagaaa
     5401 cgggatagta atgcgatgct ttgcacgaag tctccatttt attcactatc tttgcctaca
     5461 gaacgtaaaa gttaatctgg atcaaacaca tgagaaatat cctgtttacc gttttattta
     5521 taggaatact atctttaatc gcatctccta tctcactcgc gtctaaatct ttgcataagg
     5581 caaatatgat taatttgtcg aacaatgcca ttatttgcat gcatcaagat ccggatggct
     5641 atttgtggat cgggacttac gacggactta acttatacaa cggaaaagat acgtatgtat
     5701 atcgtttcga attaaataac aaaaactctc tttgtagtaa tatcattcac aaaatatccg
     5761 acgcagaacc cggcttttta tggatatcaa cctctttagg tatcaataaa ttctcgctaa
     5821 agaaacggaa agtcaccgag tcttacccgg gatacatgga gagtgatctg gtagcgaccg
     5881 attcatccgg tatcacatta gccatttgta aagaaaaccg gatatcttgt tacaccccat
     5941 ttagcgatgg attccgggat ctgccgagag aagggatagg ccctcaaacc gtacggactt
     6001 tattcgccgg aacagacgga tatttttatt taccctaaaa tccgcaacta tcatccaata
     6061 cacgattaag ggtagattta tga
//
