LOCUS       c00136_gnlPro..         5397 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GHBKLMDF_136.
ACCESSION   c00136_gnlPro..
VERSION     c00136_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 00:43:11
            Original ID  :: gnl|Prokka|GHBKLMDF_136
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5397
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5397
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[576:5397]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [PFOR_II, POR, POR_N]) and
                     (minimum(2, [ACD1_car, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     Butyrate_kinase, CT_Propionyl_CoA1, CT_Propionyl_CoA2,
                     CT_Propionyl_CoA3, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_2_CAF,
                     CoA_trans_6_Glutaconate, E1_dh, ECH_1, ECH_2, HBT, HBT_2,
                     HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C, Thiolase_N,
                     adh_short, adh_short_C2]) or FA_synthesis) and not
                     (Gly_radical_scatole or Gly_radical or Gly_430_pcre or
                     Gly_8-9_TMA))"
                     /neighbourhood="10000"
                     /product="OD_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      577..5397
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [PFOR_II, POR, POR_N]) and
                     (minimum(2, [ACD1_car, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     Butyrate_kinase, CT_Propionyl_CoA1, CT_Propionyl_CoA2,
                     CT_Propionyl_CoA3, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_2_CAF,
                     CoA_trans_6_Glutaconate, E1_dh, ECH_1, ECH_2, HBT, HBT_2,
                     HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C, Thiolase_N,
                     adh_short, adh_short_C2]) or FA_synthesis) and not
                     (Gly_radical_scatole or Gly_radical or Gly_430_pcre or
                     Gly_8-9_TMA))"
                     /neighbourhood="10000"
                     /product="OD_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..5397
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [PFOR_II, POR, POR_N]) and
                     (minimum(2, [ACD1_car, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     Butyrate_kinase, CT_Propionyl_CoA1, CT_Propionyl_CoA2,
                     CT_Propionyl_CoA3, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_2_CAF,
                     CoA_trans_6_Glutaconate, E1_dh, ECH_1, ECH_2, HBT, HBT_2,
                     HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C, Thiolase_N,
                     adh_short, adh_short_C2]) or FA_synthesis) and not
                     (Gly_radical_scatole or Gly_radical or Gly_430_pcre or
                     Gly_8-9_TMA))"
                     /kind="single"
                     /product="OD_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5397
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="OD_fatty_acids"
                     /region_number="1"
                     /rules="(minimum(1, [PFOR_II, POR, POR_N]) and (minimum(2,
                     [ACD1_car, ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (Gly_radical_scatole or Gly_radical or Gly_430_pcre or
                     Gly_8-9_TMA))"
                     /tool="gutsmash"
     CDS             complement(158..379)
                     /locus_tag="ctg136_1"
                     /transl_table=11
                     /translation="MRHNSHKYAGVPLDNPIFNRRAARNDISKAQLAVRSQQLAVTQTV
                     QAKYEFVFRRRFPISTAGSRCNSENGRF"
     CDS             complement(577..1872)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: AcetylCoA_hyd_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: AcetylCoA_hydro"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg136_2"
                     /sec_met_domain="AcetylCoA_hyd_C (E-value: 1.4e-63,
                     bitscore: 201.9, seeds: 181, tool: rule-based-clusters)"
                     /sec_met_domain="AcetylCoA_hydro (E-value: 8.2e-22,
                     bitscore: 66.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHYTTAAEAVKLIRSNDSVYIQGSTSIPETLVAAMAERGGELEHV
                     TVYSAFAVGAFDAPYCRPEYKESFLVNSLFVANNIRRWLADGYGQTIPAFLGEIPALFR
                     DGMLPLDVALINVSPPDAEGYCSFGVSADLAVSAVECAKTIIAQVNKAMPYSYGDAVIH
                     VSRLAAAVEVDSPLVEVPTAVPSETERKIGSYIAELIPDGATLQIGVGGIPNAVLAALG
                     GHRHLGLHTEAMTDGVLPLLESGVIDNSQKAVMPGVTVASLALGSRRLYDYMDYRKDLV
                     MKDVAWTNDPFRIRQNPKVMAINSAVEVDLTGQVCADSVGMRIISGVGGQHDFMYGGAL
                     SEGGKTFIAIPSTTPKGESKIRALLSPGAGVVTTRHMVQHVVTEYGVAHLRGRNLAERA
                     RALIAVAHPSVREELERAAAERFGYSFLRLKA"
     CDS             complement(1877..3187)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg136_3"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.6e-22, bitscore:
                     67.0, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIPFDRSCLRSALERMDIADIAQATIRQSGDIARTMERDTGVEF
                     LHLEMGVPGLPPERAGVEAECAALQAGVASQYPNMFGIPELKTQASRFLKAFLDVEVAP
                     RGCIPTVGSMQGSFTTFLLCSQLDPQRRKILFIDPGFPVQRSQVRILGIPSESFDIYDY
                     RGEKLGPKIESVLAAGDVAAIVYSNPNNPAWICLTEDELRTIGELATRYDAIVIEDLAY
                     LCMDFRKPLGRPFEAPYQASVARYTDNYILLVSGSKIFSYAGQRIAVAAISDALFRREY
                     PALRRRYNIGRLGDAYVLVFLYAASSGTSHSAQHALAAMFRAAADGELDFVGEASEYAR
                     RARLTKETFLRHGFRIVYDKDRDEPVSDGFFYTVGYGAMTSAELLSELLLYGICAISLT
                     STGSRQSGIRVCVSQMNRPEQFEMLEERLTAFAENNR"
     misc_feature    complement(1904..1906)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="gutsmash"
     CDS             complement(3184..3771)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: POR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg136_4"
                     /sec_met_domain="POR (E-value: 5.2e-40, bitscore: 125.8,
                     seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRDIILSGVGGQGILSIAAVIGRAALEDGLHIKQAEVHGMSQRG
                     GDVQSNLRISSSAIRSDLIPRGAADLIVSMEPMEALRYLPWLADDGWVITGITPLINIP
                     NYPEGETLRRELEKLPHAVVLDADAVAKAAMAPRSANMALLGAAAPLLGIDAAKLESGI
                     RAIFARKGGQVVETNLAAFHAGYEAAQKQAKP"
     CDS             complement(3778..5397)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     OD_fatty_acids: POR_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg136_5"
                     /sec_met_domain="POR_N (E-value: 7e-19, bitscore: 56.8,
                     seeds: 31, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.3e-20, bitscore:
                     62.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SEPDRKLMAKRELLLGDEALALGALHAGLSGVYAYPGTPSTEITE
                     YIQQHPLAAQRNVHRTWSSNEKTAMEEALGMSFAGKRALVCMKHVGLNVAADAFVNSAM
                     TGANGGLVVVAADDPSMHSSQNEQDSRFYGKFALVPTFEPADQQEAYDMAKAAFDLSER
                     FRIPVLMRLTTRMAHSRAVVETGEARAENPLSHPEQPRQWVLMPGNSRVRYQTLLEDYA
                     RLEEESAASPFNAYTEGPDKRLGIVACGIAHNYLMENYPDGCPHPVLKIAQYPLPRTLV
                     RRIAAECGTLLILEEGQPVVEEIVRGVLPNPVAIRGRLTGELPRTGELTPDSVRAALGM
                     APRRTHAASGIVVPRPPALCQGCGHRDMYTALTEVIREEYPAGKVFSDIGCYTLGWLAP
                     LHAIDTCVDMGASITMAKGAADAGQHPSVAVIGDSTFTHSGMTGLLDAVNERADITVII
                     SDNLTTGMTGGQDSAGTGRLEQICAGIGVDPAHIRTVVPLPKNREEMKAVIREEIAHRG
                     VSVIIPRRECIQTAKRHNAAKK"
     misc_feature    complement(5356..5358)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="gutsmash"
ORIGIN
        1 ggataaacac agatcatcag tgacgcacga aggacttttc tgccggagaa ggtccgggat
       61 ccaatccggc agccgcccgg attattcagg aacttgatgt tccgggtagc agcgagggtg
      121 accgttttag gtcacccttt ttgtttgaat tcgtttatca gaatctcccg ttttcagagt
      181 tgcaacggct tcccgcggtg gaaatcggga atcttcgacg gaaaacgaac tcgtatttgg
      241 cctgcacggt ttgggtgacg gcgagttgct ggctgcgcac ggcgagctgc gccttgctga
      301 tgtcgttgcg cgcggcccgg cggttgaaga tcgggttgtc gagcgggacg ccggcgtatt
      361 tgtggctgtt atggcgcatc tgtgtcccgg acggccaccg cgtgttccga gccttcgggc
      421 cgcagccgct tcgcgggggc ggttcccggt tccccgggcg catgacggat cacgccggtc
      481 gtgcggcggc cgtcgggggc gaaaaagagg tgattgcgga atatctgcct atcggtaaaa
      541 aggcccgccc ggtcttttcg gggaccgggc cgggctttat gctttgaggc gcaggaacga
      601 atagccgaag cgttcggctg cggcgcgttc cagctcctcg cggaccgagg ggtgcgccac
      661 ggctatcagc gctcgggcgc gttcggcgag gttgcggccc cgcaggtgcg ccacgccgta
      721 ttcggtgacg acatgctgga ccatgtggcg cgtcgtgacg acgcctgcgc cgggcgacag
      781 cagcgccctg atcttcgatt cgcccttggg cgtggtcgag gggatggcga tgaaggtctt
      841 gccgccttcg gacagcgctc cgccgtacat gaaatcgtgc tggccgccca cgcccgagat
      901 gatgcgcatt ccgaccgagt cggcgcacac ctgccccgtg aggtccacct ccacggccga
      961 attgatggcc atgactttcg ggttttgccg gatgcggaac gggtcgttgg tccatgccac
     1021 gtccttcatc acgaggtctt tgcggtagtc catgtagtcg tacagccggc gcgagccgag
     1081 ggccagcgag gccaccgtca cgccgggcat caccgccttc tgcgagttgt cgatcacgcc
     1141 gctttccagc agcggcagca cgccgtcggt catcgcctcg gtgtgcagtc ccagatgccg
     1201 gtggccgccg agggccgcca gtacggcgtt cggaatgccg cccacgccga tctgcaacgt
     1261 cgccccgtcg ggaatcagtt cggcgatgta gctgccgatt ttccgttccg tttcgctcgg
     1321 cacggccgtc gggacctcca ccagcgggga gtcgacctct acggccgccg ccaggcgcga
     1381 gacatgaatc acggcgtcgc cgtaagagta gggcatcgcc ttgttgacct gcgcgatgat
     1441 ggtcttggcg cactccacgg cggaaaccgc cagatcggcc gagacgccga acgagcagta
     1501 gccttcggcg tcgggcggcg agacgttgat gagcgcgacg tcgagaggca gcatgccgtc
     1561 gcggaacagc gcggggattt cgcccaggaa ggccggaatg gtctgtccgt agccgtcggc
     1621 cagccaccgg cggatgttgt tggcgacgaa caggctgttg acgaggaacg actccttgta
     1681 ttcggggcgg cagtagggag cgtcgaaggc tcccacggcg aaggccgaat agaccgtcac
     1741 atgctccagt tcgccgcccc gttcggccat tgcggccacg agcgtttcgg ggatcgaggt
     1801 gctgccctga atgtagacgc tgtcgttgga tcggatgagt tttaccgcct ccgcggcagt
     1861 cgtgtagtgc atgttgtcag cggttatttt ctgcaaaagc ggttaaacgt tcttcgagca
     1921 tttcgaactg ttcggggcgg ttcatctgcg agacgcagac gcggatgccg ctctggcggc
     1981 tgccggtcga ggtgagcgag atggcgcaga tgccgtagag cagcagctcg ctcaacagtt
     2041 cggcgctggt catcgcgccg tagccgaccg tgtagaagaa tccgtcgctg accggctcgt
     2101 cgcggtcctt gtcgtagacg atgcggaatc cgtggcggag gaacgtctcc ttggtcagcc
     2161 gggcgcgccg ggcgtactcc gaggcttcgc ccacgaagtc cagttcgccg tcggccgccg
     2221 cgcggaacat tgccgctagg gcgtgctgcg ccgaatggct cgtgccggat gaggccgcat
     2281 agagaaagac cagtacgtag gcgtcgccca gccgtccgat gttgtaacgc cggcgcaggg
     2341 cggggtactc gcggcggaaa agcgcatcgg agatcgccgc cacggcgatt cgctgcccgg
     2401 cgtagctgaa tatcttcgac ccggagacca gcaggatgta attgtccgtg tagcgggcca
     2461 ccgaagcctg gtagggggct tcgaaaggcc gtcccaacgg tttgcggaaa tccatgcaga
     2521 ggtaggcgag gtcctcgatg acgatggcgt cgtaccgtgt ggccagctcg ccgatcgtgc
     2581 gcagttcgtc ctccgtgagg cagatccacg ccgggttgtt ggggttcgag tagacgatgg
     2641 ccgccacgtc gcctgcggcg aggacgctct cgatcttcgg ccccagcttt tcgcccctgt
     2701 aatcgtagat gtcgaacgac tccgagggaa tgccgagaat gcgtacctga ctgcgctgca
     2761 cggggaatcc ggggtcgatg aagaggattt tgcgccgctg cgggtcgagc tgcgagcaga
     2821 gcaggaatgt ggtgaagctg ccctgcatcg acccgacggt ggggatgcag ccccgggggg
     2881 ccacctccac gtcgaggaag gctttcagga agcgcgacgc ctgcgtcttg agttccggaa
     2941 tgccgaacat gttgggatac tgcgacgcga cgcccgcctg cagggccgcg cattcggcct
     3001 cgacgcccgc ccgttcgggc ggcagccccg gcacgcccat ttcgaggtgg aggaactcca
     3061 cgcccgtgtc gcgctccatc gtgcgggcga tgtcgcccga ctggcggatc gtggcctgcg
     3121 cgatgtcggc tatgtccatc cgctccagtg cggagcgcag gcaggacctg tcgaacggta
     3181 ttttcatggc tttgcctgtt tttgggccgc ttcgtagccg gcgtggaacg ccgcgagatt
     3241 cgtttcgacg acctgcccgc ccttgcgggc gaagatggcg cggatgccgg actccagttt
     3301 cgcggcgtcg attccgagca gcggcgcggc agctcccagc agcgccatgt tggccgagcg
     3361 gggcgccatg gcggctttgg cgacggcgtc ggcgtcgagg actacggcgt gcgggagttt
     3421 ttccagttcg cgccgcaggg tctcgccctc cgggtagttc gggatgttga tcagcggcgt
     3481 gatgccggtg attacccatc cgtcgtcggc gagccacggc aggtagcgca gcgcctccat
     3541 cggctccatc gagacgatga gatcggccgc gccgcgcgga atgagatccg aacggattgc
     3601 cgacgacgaa atgcgcaggt tcgactgcac gtcgccgccg cgctgactca tgccgtggac
     3661 ctcggcctgc ttgatgtgga gcccgtcttc aagggcggcc cgtccgatga cggcggcgat
     3721 cgagaggatt ccctggcctc ctacgcccga gagtatgatg tcgcgtttca taagccttta
     3781 ctttttagca gcgttgtgtc gctttgcggt ctggatacat tctcttcgcg ggatgatcac
     3841 cgagacgccc cggtgggcga tctcctcgcg gatcacggcc ttcatctcct cgcggttctt
     3901 gggcaggggc acgaccgtgc ggatatgtgc gggatcgacg ccgatgccgg cgcagatctg
     3961 ttccagacgg cccgttccgg ccgaatcctg tccgccggtc atgcccgtcg tgaggttgtc
     4021 cgagatgatg accgtgatgt ccgcgcgctc gttcacggcg tccagcaggc ccgtcatgcc
     4081 cgaatgggtg aaggtcgagt cgccgatcac ggccaccgaa gggtgctggc ccgcgtcggc
     4141 cgcgcctttg gccatggtga tcgaggcccc catgtcgacg catgtgtcga tggcgtgcag
     4201 cggcgccagc caccccagcg tgtagcagcc gatgtcgctg aaaaccttgc ccgcggggta
     4261 ttcctcgcgg atcacctccg tcagggctgt gtacatgtcg cggtgtccgc atccctggca
     4321 gagcgcgggc gggcggggta cgacgattcc gcttgcggcg tgcgtccggc ggggcgccat
     4381 gccgagggcc gcgcgcacgc tgtcgggcgt cagctccccg gtgcggggca gttcgccggt
     4441 caggcgtccg cggatcgcca cgggattggg cagcacgccc cggacgatct cttccacgac
     4501 gggctggccc tcttcgagga tcagcagcgt gccgcactcg gcggcgatac ggcgcaccag
     4561 cgtgcggggc agcgggtact gcgcgatttt cagcaccggg tgcggacatc cgtcggggta
     4621 gttctccatc aggtagttgt gtgcgatgcc gcaggcgacg atgcccagcc gtttgtccgg
     4681 accctccgta taggcgttga acgggctggc ggcggactcc tcttccagcc gggcgtaatc
     4741 ctccagcagc gtctggtatc gcacgcgcga gtttcccggc atcagcaccc actggcgggg
     4801 ctgttcggga tggctcaacg ggttttcggc gcgcgcctcg cccgtctcga cgacggcccg
     4861 cgaatgggcc atgcgggtcg tcaggcgcat cagcaccgga atccggaacc gttccgagag
     4921 gtcgaaggcg gccttcgcca tgtcgtatgc ctcctgctgg tccgcaggct cgaaggtcgg
     4981 cacgagggcg aatttcccgt agaagcgcga atcctgttcg ttctgcgacg agtgcatcga
     5041 ggggtcgtcg gctgccacga ccaccagtcc gccgttggct ccggtcatcg ccgagttgac
     5101 gaaggcgtcg gccgccacgt tcagccccac gtgtttcatg cagaccagcg cgcgcttgcc
     5161 ggcgaacgac atgccgagcg cctcctccat ggccgtcttt tcgttcgacg accacgtgcg
     5221 gtgcacgttg cgctgcgcag ccaggggatg ctgctggatg tattccgtga tttcggtcga
     5281 gggtgtgccc ggataggcat agacacccga cagtcccgcg tggagcgccc ccagtgcgag
     5341 ggcttcgtct ccgagtaaga gttctcgttt tgccatgagc ttacggtctg gttcact
//
