LOCUS       c00133_gnlPro..         7047 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KCMHMAGB_133.
ACCESSION   c00133_gnlPro..
VERSION     c00133_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:58:51
            Original ID  :: gnl|Prokka|KCMHMAGB_133
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7047
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1077
                     /locus_tag="ctg133_1"
                     /transl_table=11
                     /translation="GHPLSQAVVESLGGERDVPVTGFESIPGQGVRGVAEGRTWYAGND
                     ALLSRHGIVPDARLQRLAEGWMQEAKTVVWFADEERTRAVLALADALKPSSAGAVARLH
                     ALGITVWMLTGDNAGSARETARKAGITRFRAGVLPHEKAGFVRQLQSEGRTVAMAGDGI
                     NDSAALAQADLSVAMGQGSDIAMDTAMVTILSSDLRKIPEMIRLSQLTVRTIRQNLFWA
                     FIYNLVAIPVAAGALYPLWGFLLDPMIGGAAMALSSVSVVANSLRLKRRRIGDECEHDE
                     PVETEEKMKKEFIVDGMTCNHCRMHVEHALNTLDGVKAVVTLDPPVAAVEYSGPEVGLG
                     ELQKAVTERAGEYTLRAR"
     protocluster    1..7047
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3877:6927]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3878..6927
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7047
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7047
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1194..2597
                     /locus_tag="ctg133_2"
                     /transl_table=11
                     /translation="MLMETLSRWIGGVNDVLWTYVLVAALLGCAVWFTLRTRGVQFRLL
                     GEMMRVLGESAGSGPKGERHVSSFQAFAVSLASRVGTGNLAGVATAIVVGGPGAVFWMW
                     VIALVGAASAFVESTLAQLYKRRGRDSFIGGPAYYMERGLGRRWMGVLFAVLISVTFGF
                     AFNSVQSNTICAAWEGAFGADHVWVGVVLTALTLLIIFGGIRRIARVSGVIVPIMALGY
                     IVLALGVVLFNLGRLPEVLELIVADAFGWEQTLGGAVGAALMQGIKRGLFSNEAGMGSA
                     PNVAATAHVSHPVKQGLIQTLGVFTDTLVICTCTAFIILFGGVPDASLNGIQLTQAALE
                     SEIGPAGGGFVAVAIFLFAFSSIIGNYYYGEANIRFITPRPWALTLYRLLVGAMVLFGS
                     VATLDLAWSLADVTMALMTLCNLAAIVLLGRQAFLLLADYTAQKRQGIKNPVFTKDRIP
                     ELKDKAECW"
     CDS             complement(2602..3756)
                     /locus_tag="ctg133_3"
                     /transl_table=11
                     /translation="MKNRLPDLNVPARERIGWVDLLRVIACFLVVFSHSCDAFVAVFDS
                     DRATFLQGALAGSFVRACVPLFVMMSGVLLLPVRTGTGAFYRKRIGRVLLALVFWSLTL
                     PVLYYLYMRYVGTSSPSIAPALFTGEATLHKMWTFVFNFCYDTTPLWYLYMLIGLYLIM
                     PLISPWLERASRRELQSVLAIWGVTLLLPYVKMLAPALGYMGNYGNTGLYGVCDWNEFG
                     TFHYVSGFAGYLVLAFYLVKFPPAWNWRKTLGICIPTFLAGYLATGLGYVVMQKHFPGN
                     YAYLEIVWYFAGINVFMMTAPVFILVQKAAARPRAWLSRLAGATFGIYLCHFIFVQAGY
                     DLVQRIPGLPALARIALIACGAFAVSWAVVRLMQRWSVTRRLVE"
     CDS             complement(3878..4603)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 69.5;
                     E-value: 1.2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_4"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-28, bitscore:
                     88.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAGAG
                     TLTDPAEILRMAVDQKPFFGRRGGVTFSGGEPTFQAAELVPLVKSLKEAGIHVCIDSNG
                     GVWNPAVEELLGLVDLVLLDVKQADPERHRALTGRENAQTLRTAAWLEAHGKPFWLRYV
                     LVPGISDAEADIRALGERLGGYKSVERVELLPYHTLGVHKYEAMGLEYKLRDVRENTPE
                     QLDRAAALLREYFPTVVVN"
     CDS             complement(4699..6927)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_5"
                     /sec_met_domain="Gly_radical (E-value: 8.6e-47, bitscore:
                     146.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1167.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFIDGLWSKEINVTSFVQTNITPYLGDASFLAGPTERTKH
                     IWNLCLKALEEERQHNGVRALDNKTVSTVTSHKAGYIDRENELIVGLQTDELLKRAIKP
                     FGGINVVSRACKENGVEVDEKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGTDRLIEAKQEDLRNLTGPMTDARIRLREEVAEQIKALKDIR
                     TMGEYYGLDLSRPAHSAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GTIDESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTILWSPELPQGFKDFCAKVSADTSSIQYENDDLMREVRHS
                     DDYGIACCVSFQDIGRQIQFFGARTNLAKALLLAINEGRCENTGTLMVKGIPALSEGPL
                     RFEEVMRNYKMVLKEIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIALDSLSAIKYARVTPRRNAEGLTEAFDIEGEFPCYGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRLKGVAFAPGANPMHGRDKSGAIAS
                     LASVAKMRYRDSQDGISNTFSIVPKSLGPTAGERIENLVTMLDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFTKLSREHQLEVISRSFHERM"
ORIGIN
        1 gggcatccgc tttcgcaggc cgtcgtcgaa tcgctcggcg gggagcggga cgttcccgtg
       61 accgggttcg aaagcattcc cgggcagggt gtccggggcg tcgcggaggg gcggacctgg
      121 tatgccggga acgacgcgct gctgtcccgc cacggcatcg tgcccgacgc ccgtctgcaa
      181 cggctggccg aagggtggat gcaggaggcg aagaccgtgg tttggttcgc cgacgaggag
      241 cggacgcgcg ccgtgctcgc ccttgccgat gcgctgaaac cctcgtcggc cggggccgtc
      301 gcccggctgc acgcgctggg aatcacggtc tggatgctga cgggcgacaa cgccggttcg
      361 gcgcgcgaaa cggcccggaa agccgggatc acccgtttcc gggcgggcgt cctgccgcac
      421 gaaaaggccg gattcgtccg gcagttgcag tccgaaggcc ggacggtggc catggccggc
      481 gacgggatca acgacagcgc ggctctcgcg caggccgatt tgagcgtcgc catggggcag
      541 gggagcgaca ttgcgatgga taccgcgatg gtgacgatcc tctcgtcgga cctccggaaa
      601 atcccggaga tgatccggct gtcgcagctc accgtgcgga cgattcgcca gaacctcttc
      661 tgggccttca tttacaacct cgtcgccatt cccgtagcgg ccggggcgct ctatcccctc
      721 tgggggtttc tgctcgaccc gatgatcggc ggggcggcca tggccttgag cagcgtgagc
      781 gtcgtggcca acagcttgcg gctgaaacgc cggcggatcg gcgatgagtg tgaacatgac
      841 gaaccggttg aaaccgaaga aaagatgaaa aaggaattta tcgtggatgg catgacgtgc
      901 aatcattgcc ggatgcatgt ggaacatgcg ctgaacaccc tcgacggcgt gaaggcggtc
      961 gtgacgctgg accctccggt ggccgccgtg gagtactccg ggccggaagt cggcctcggg
     1021 gagttgcaga aggccgttac ggagcgggcc ggggagtata ccctgcgggc gcggtgacgg
     1081 cggacgcccc tgcgcaggcg gggcgttttt ttcgtgccgc gccgcatctt tccggcgaat
     1141 gtccggcgga aaatgcgttt tagcattaac tttgctgtcg gatcgtgtaa agtatgctga
     1201 tggagactct ttcccgttgg atcggcggcg tgaacgacgt gctgtggacc tatgtgctgg
     1261 tcgcggcgct cttggggtgt gccgtgtggt tcaccctgcg cacgcggggc gtgcagttcc
     1321 gcctgctggg cgagatgatg cgcgtgctgg gcgagtcggc cggaagcggc ccgaagggcg
     1381 agcggcacgt ctcgtcgttc caggctttcg ccgtgtcgct ggccagccgc gtcggcacgg
     1441 ggaatctcgc gggcgtcgcc acggccatcg tcgtcggggg acccggcgcg gtcttctgga
     1501 tgtgggtcat cgcccttgtg ggcgccgcga gcgctttcgt cgagtcgacc ctcgcacagc
     1561 tttacaaacg ccgcggcagg gactctttca tcggcggtcc ggcctactac atggaacgcg
     1621 gcctcgggcg gaggtggatg ggcgtgctgt tcgccgtgct gatctccgtg accttcggat
     1681 tcgccttcaa ctcggtgcag agcaacacga tctgcgccgc gtgggaggga gctttcggcg
     1741 ccgatcatgt gtgggtgggc gtcgtgctca cggcgctgac gctgctcatc atcttcggag
     1801 gcatacggcg cattgcgcgt gtcagcggcg tgatcgtccc gattatggcg ctggggtaca
     1861 tcgtgctggc gctgggcgtc gtgctgttca acctcgggcg gctgcccgaa gtgctggaac
     1921 tgatcgtcgc cgacgctttc ggctgggagc agacgctggg cggcgcggtc ggcgcggcgt
     1981 tgatgcaggg catcaagcgg ggacttttca gcaacgaggc cggtatgggt tcggccccga
     2041 acgtcgccgc cacggcccac gtttcgcacc ccgtgaagca gggacttatc cagaccctcg
     2101 gggtcttcac cgatacgctc gtcatctgca cctgcacggc cttcatcatc ctgttcgggg
     2161 gcgttcccga cgcttcgctc aacggcattc agctgacgca ggcggctctg gagagcgaga
     2221 tcggtcctgc gggcgggggg ttcgtcgccg tggcgatctt cctgttcgcc ttttcgtcga
     2281 tcatcggcaa ttattactac ggcgaagcca acatccgctt catcactccg cggccgtggg
     2341 cgctgacgct ctaccggctg ctggtcggcg cgatggtgct cttcggatcc gtggcgacgc
     2401 tcgatctggc atggagcctg gccgatgtga cgatggcgct gatgacgctg tgcaatctgg
     2461 cggcgatcgt gctgctgggc cgtcaggcct tcctgctgct ggccgactac acggcccaga
     2521 agcggcaggg catcaaaaat ccggtcttca cgaaagaccg gattcccgaa ttgaaagaca
     2581 aggccgagtg ctggtagcgc attactccac cagccggcgc gtcacgctcc accgctgcat
     2641 cagccgcacc accgcccagc tcacggcgaa ggctccgcag gcgatcagcg cgatgcgcgc
     2701 cagcgcggga agtcccggaa tccgctgcac gaggtcgtag ccggcctgaa cgaagatgaa
     2761 gtggcagaga tagatgccga acgtcgcgcc cgccagccgc gagagccatg cgcggggccg
     2821 tgcggcggcc ttctgcacga ggatgaagac cggggcggtc atcataaaga cgttgatgcc
     2881 ggcgaaatac cagacgattt cgaggtaggc gtagttgccc ggaaaatgtt tctgcatcac
     2941 cacgtaaccc agccccgtgg cgaggtatcc ggcgaggaag gtcgggatgc agatgcccaa
     3001 tgtcttgcgc cagttccatg cgggcgggaa tttcacgagg tagaaggcca gtacgaggta
     3061 accggcgaag cccgagacgt agtggaacgt gccgaactcg ttccagtcgc agacgccgta
     3121 aagccccgtg ttgccgtaat tgcccatgta gccgagcgcc ggggccagca tcttcacgta
     3181 gggcagcagc agcgtcacgc cccagatcgc gaggacgctc tgcaactcgc gccgcgaggc
     3241 ccgttcgagc cacgggctga tcagcggcat gatcagataa agtcctatca gcatatagag
     3301 ataccagagc ggcgtggtgt cgtagcagaa gttgaagacg aaggtccaca tcttgtgcag
     3361 cgtcgcttcg ccggtgaaca gcgcgggggc gatcgacggg ctggaggtcc cgacgtagcg
     3421 catatacagg tagtagagca cgggcagcgt cagcgaccag aagaccagcg ccaggaggac
     3481 gcggccgatg cgcttgcggt agaaagcccc cgttccggtc cgcacgggca gcagcagcac
     3541 gcccgacatc atcacgaaca gcggcacgca ggcccggacg aaactgcctg ccaacgctcc
     3601 ttgcaggaag gtggcgcggt cgctgtcgaa cacggcgacg aaggcgtcgc agctgtggga
     3661 gaagaccacc aggaagcagg cgatcacgcg caggaggtcc acccagccga tgcgttcgcg
     3721 ggccgggacg ttcaggtcgg gaagtcggtt cttcattgtc gcagacaatg gatttccggc
     3781 gaaggtacga aaaaaatccg ggaccgccgt gcgttttcgc cgcggcgtcc cggatttttc
     3841 gttgtccgca ctccctccgg cggacgtccc cttcgggtca gttgaccacc accgtcggga
     3901 agtattcgcg cagcagggcc gcggcgcggt cgagctgttc gggggtgttc tcccgcacgt
     3961 cgcggagttt gtactccagc cccatcgcct cgtacttatg cactccgagg gtgtggtagg
     4021 ggagcagttc gacccgctcg accgacttgt agccgccgag gcgttcgccc agcgcgcgga
     4081 tgtcggcttc ggcgtcgctg atgcccggaa ccagcacgta gcgcagccag aagggttttc
     4141 cgtgggcttc gagccaggcg gccgtgcgga gcgtctgcgc gttttcgcgt ccggtgaggg
     4201 cgcggtggcg ttcgggatcg gcctgcttca cgtccagcag caccaggtcg accagcccca
     4261 gcagctcctc gacggccggg ttccacaccc cgccgttcga gtcgatgcag acgtggatgc
     4321 cggcctcctt caacgacttt acgagcggaa cgagttccgc ggcctggaag gtgggttccc
     4381 cgcccgagaa ggtcacgccg ccgcgccgcc cgaagaaggg cttctggtcg acggccatgc
     4441 gcaggatctc cgcagggtcg gtcaatgtgc ccgctccggc ctcgatcgtg tcggggttgg
     4501 cgcagtagag acagcggaaa ttgcagcctt ggaggaaaac gacgagccgg agtcccggcc
     4561 cgtcgaatgt tcccatggat tcgtaagaat gtacacgaag catgattgtt ccttgttttt
     4621 gaataagggg gttcgggcgc aaaccgggtt ccggtatgca aatcctgcgc ccgcccctaa
     4681 aagcggcttt tcaagccgtt acatcctttc gtggaagctg cggctgatga cttcgagctg
     4741 gtgttcgcgg ctcagcttgg tgaagttcac ggcgtagccc gagacgcgga tcgtcagctg
     4801 cggatatttc tcggggtgct ccatggcgtc ttcgagcatc tcgcggttca gcacgttgac
     4861 gttcaggtgg tgtgcgccct tggtgaaata gccgtcgagc atcgtcacga ggttctcgat
     4921 acgctctccg gccgtcggac cgagcgattt gggcacgatc gagaaggtgt tcgaaatacc
     4981 gtcctgcgag tcgcggtaac gcatcttggc caccgaggcg agcgaagcga tggcgccgct
     5041 cttgtcgcgg ccgtgcatcg ggttggctcc cggggcgaag gccacgccct tgaggcgtcc
     5101 gtcgggcgtt gcgccggtct tcttgccgta catcacgttc gaggtgatcg tcagcagcga
     5161 cagcgtcggg cgggcgttct tgtagacggg cagcttcttc agctcctcgc tgaagtagta
     5221 gacgaggtcc acgcccaggt ggtcgacacg gtcgtcgttg ttgccgtagc aggggaattc
     5281 accctcgatg tcgaaggctt cggtgagccc ctcggcgttg cggcggggcg tcacgcgggc
     5341 gtatttgatg gccgagagcg agtcgagggc gatcgagaga ccggccacgc cgtaggcgag
     5401 attgatgcgg ggatcggtgt cgacgaatgc catctgggcc ttctcgtagt agtacttgtc
     5461 gtgcatgtag tggatgatgt tcatggcctc gttgtagacg cgggcgatct ccttgagcac
     5521 catcttgtag ttgcgcatca cctcctcgaa gcgcagcgga ccctccgaca gcgccggaat
     5581 gcccttgacc atcagcgtgc cggtgttctc gcagcgacct tcgttaatgg ccagcagcag
     5641 ggccttggcc aggttggtgc gggcgccgaa gaactggatc tggcggccga tgtcctggaa
     5701 cgagacgcag caggcgatgc cgtagtcgtc cgagtggcgc acttcgcgca tcaggtcgtc
     5761 gttctcgtac tggatcgagc tggtgtcggc cgaaaccttg gcgcagaagt ccttgaatcc
     5821 ctgcggcagc tcggggctcc agagaatcgt gaggttcggt tcgggcgaag gaccgaggtt
     5881 gtagagcgtt tgcaggaagc ggaacgaggt cttcgtcacc ttcgtgcgac cgtcgttgaa
     5941 gcgtccgccg atggcctcgg tcacccacgt cgggtcgccg gcgaagatgt cgttgtagga
     6001 ctgcatgcgc aggtggcgca ccatgcgcag cttgatgacg aactggtcga tcagctcctg
     6061 ggcgaacgac tcgtcgatcg tcccgtgcgc gaggtcgtat tcgatgaaga tgtcgaggaa
     6121 cgacgatacg ttgcccagcg acatggcggc tccgtcctgc tccttgacgg cggccagata
     6181 ggccatgtag acccactgca cggcctcctg cgccgaatgc gccgggcggc tgaggtccag
     6241 tccgtagtat tcgcccatcg tgcggatgtc cttcagggcc ttgatctgct cggcgacctc
     6301 ttcgcgcagg cggatgcggg cgtcggtcat cgggccggtg aggttgcgga ggtcctcctg
     6361 cttggcctcg atcaggcggt cggttccgta gagggccaga cggcggtagt cgccgatgat
     6421 gcggccgcgg gcgtagttgt cgggaagacc cgtcaggaag cccagcgagc ggaacgagcg
     6481 gatctcctcg gtgtagacgt cgaacacgcc gtcgttgtgc gtcttgcggt agtgggtgaa
     6541 gatgtccttc accttctcgt cgacctccac gccgttctcc ttgcaggcgc gcgatacgac
     6601 attgatgccg ccgaaaggtt tgatggcgcg cttgagcagt tcgtcggttt gaaggcccac
     6661 gatcagctcg ttttcgcggt cgatgtagcc ggccttgtgc gaggtgacgg tcgaaacggt
     6721 cttgttgtcc agcgcgcgga cgccgttatg ctgacgctcc tcttcgaggg ccttgaggca
     6781 gaggttccag atgtgcttcg ttcgctcggt aggtcctgcg aggaacgagg cgtcgcccag
     6841 gtagggggtg atgtttgtct gtacgaagct cgtcacgttg atctctttgc tccaaagccc
     6901 gtcgataaag gttttgttca gttccataga tgatattgtt cgttttgaga tgataattgt
     6961 cctatttcgg tcggcaaaga tacgttattt ttttcacacc ggccaaagag cggttacaat
     7021 tttttataaa aatttaaaaa ataccta
//
