LOCUS       c00146_gnlPro..         5044 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CENFGCCL_146.
ACCESSION   c00146_gnlPro..
VERSION     c00146_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:19:12
            Original ID  :: gnl|Prokka|CENFGCCL_146
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5044
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5044
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1358:4816]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1359..4816
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5044
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5044
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(68..853)
                     /locus_tag="ctg146_1"
                     /transl_table=11
                     /translation="MVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSADGFRDYFVQLT
                     ECEPARAAKFGMDHYTWLCINPKEAQDVAFARDVMKLIPEFIDRPNVLGIGEIGLNRNT
                     SHEMTIFEEHLDLAVRLNQLVLIHTPHLEDKLKGTVMILSALKNRPDLDPCRVLVDHCE
                     EHTFPLVKEAGYWAGLTLYPTSKLNPKRAADILELYGMDRVWANSAADWGDSDPLALTA
                     LACELRRRGFSRQETERLLLENPETFMGQSPKFRKVSSR"
     CDS             complement(886..1158)
                     /locus_tag="ctg146_2"
                     /transl_table=11
                     /translation="MKRSKKEVLDADFAATRAGVLDVAAFLDRVDRGEGEADFRYHALM
                     DVLPLLSASGPGRVRAILEALSYRDDTVPETAAAKAACGAPNLSK"
     CDS             1359..2060
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_3"
                     /sec_met_domain="SucD_FumR (E-value: 2e-14, bitscore: 42.0,
                     seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGIDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             2179..4008
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 175.7; E-value:
                     5.5e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_4"
                     /sec_met_domain="FAD_binding_2 (E-value: 4.5e-55, bitscore:
                     175.7, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 8.4e-39,
                     bitscore: 121.0, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VLVVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGI
                     NAAKNYQNDGDSVYRLFYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYG
                     GLLDNRSFGGAQLKRTFYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVD
                     GEAKGIVTRNLVTGEIKSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFF
                     ANPCFTQIHPTCIPVKGDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVP
                     EEERDYYLERKYPSFGNLAPRDISSRAAKEACDDGRGVAITGKGVFLDFADEIKRMGRE
                     WIDGQYGNLFDMYEEITDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEAN
                     FSDHGANRLGASALMQGLSDGYFVIPATLPAYLTTTKPGAVTTASPEFKAAEDEVKERI
                     NKLLNVKGTKTVDSFHRELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGT
                     ADGINAELEKACRVADFLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAY
                     VAAWEYTGVDSLPVLHKEPLTFENVHLAIRSYK"
     CDS             4049..4816
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_5"
                     /sec_met_domain="Fer2_3 (E-value: 1.9e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 6e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 3.4e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENRDAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 cgtcctcacc gtctttttcc cggacgggga ctagagggcc gtaccctccg ccgcgcctcg
       61 ggcggaatta gcgggacgac actttccgga acttcgggga ctgtcccatg aatgtctccg
      121 gattttccag gagaagacgt tccgtttcct gccgggaaaa accgcgcctg cgtagctcgc
      181 aggccagggc cgtcagcgcc aggggatcgg aatcccccca gtccgccgcg gaattggccc
      241 agacacggtc cattccgtac aattccagaa tatccgccgc ccgcttggga ttcagcttac
      301 tggtgggata aagggtcagc ccggcccagt aaccggcctc cttcaccagc gggaaggtat
      361 gttcctcaca gtgatccacc agcacgcggc aagggtccag atccggacgg tttttcaggg
      421 cggaaagaat cattacggtc cccttaagct tgtcttccag atggggagtg tgaatcagca
      481 caagctggtt gaggcgcacc gccagatcca gatgctcttc aaaaatagtc atttcatggg
      541 aagtattccg gttcagccct atttccccga tgccgaggac attgggccgg tcaatgaact
      601 ccggaatcag tttcatcaca tcccgcgcga aagccacgtc ctgcgcctcc ttgggattga
      661 tgcacagcca tgtgtaatgg tccatgccga acttggccgc cctggcgggt tcgcattccg
      721 taagctgtac gaaataatcc cggaaaccgt ccgcggacga acggtcgtaa ccggcccaga
      781 acgccggttc ccctaccgcc aggcagccgg cctgcgccat ggcccggtat tcgtccgtgg
      841 tgcggcacac catatgtata tgaggttcta tgaaagccat cattcttatt tgctgaggtt
      901 cggggctccg caggccgctt tggctgcggc ggtttccggc acggtgtcat ccctgtagga
      961 aagggcttcc agaatggccc ggacgcgtcc ggggccggat gcggacaaga ggggaagaac
     1021 atccatcagg gcatggtagc ggaaatccgc ctccccttcc ccgcggtcca cccggtccag
     1081 aaatgcggcc acatccagaa cgccggcgcg cgtcgctgcg aaatccgcgt ccagcacctc
     1141 ctttttactt cttttcatgc acagacgata cgcctatgcg gaaagcctcg tcaagccgga
     1201 acaggcaaca aaatgataac cattccaaac tgataaacag caacctcttt ccccggtttt
     1261 tcaaaaatcc gaaaagcgcc ggaaattcaa gaagaggctt gccatccggg cttgaaccgg
     1321 gcatcctgtg aagacgcatt tatttcatta gagcgcctat gaatgcacta gtaaaaacca
     1381 tatgtacatt cgtgacatcc tccatcggcc gcaaaatcat tgtggctctg acggggttat
     1441 gtttggtctt gttccttgcg ggacacctgg caggcaacct tctcatcttc ggaggccctg
     1501 aatggatcaa cacctacgcc cacggcctgc actccatgcc ggaagcagcc ctctggggaa
     1561 tccgcgcggg gcttgcggtg atcttcatca tccacgtctg gctgaccatc cagctgaagc
     1621 tggaaaacca tgcggcccgc gaaccctacg tgttcaagaa caccattaag gcaacgctct
     1681 cctcccgcta catgatttac accggtttga ccgtcctggt gttcctgatc taccacctgt
     1741 accagtacac cctgcgcgtg ggatacgacc ccgcccagtt caccaccttt atttccgacg
     1801 gtacggtgga aacctttgac gtgtacaaga tgatcgtgac cggattctcc aacgtatggt
     1861 gctccgcctt ctacatcctg gccgttctga tgctgttcag ccatctgcgc cacggggtgc
     1921 agtccatctt ccagactgtg ggcattgatt cccgcaaaat ccggcccttc tacaatttta
     1981 tcgccatcgc ctacggcgcg attatctgcc tgggcttcat ctccgtgccg gtctcggttc
     2041 ttctgggtat catcaaataa ccatcaactc ctctcaaagc nnnnnnnnnn aattccctcc
     2101 ggccctattc aggacaaatg gtccaagtac aagcttgaat ccaaactgat caatccgaac
     2161 aacaagcgca agtacacggt gcttgtggta ggttccggcc tggccggcgc ctccgccgcc
     2221 gccacgctcg ccgaacttgg ctacaacgtc aaatgcttct gctaccagga cagcccccgc
     2281 cgcgcccact ccattgcggc ccagggcggc atcaacgcgg ccaagaacta ccagaacgac
     2341 ggcgactccg tttaccgcct gttttacgac accgtcaagg gcggcgactt ccgcgcccgt
     2401 gaagccaacg tttaccgcct ggcggaagtt tcctccaaca tcattgacca gtgcgtggcc
     2461 cagggcgttc cctttggccg tgaatacggc ggcctgctgg ataaccgctc cttcggcggc
     2521 gcccagctca agcgcacctt ctacgcacgc gggcagacgg gccagcagct cctgctgggc
     2581 gcttaccagg ccctggaaaa ggaagtggcc aaaggacaca ttgaaatgtt ctcccgccac
     2641 gaaatgatgg atctggtgct ggtggacggc gaagccaagg gcatcgtcac ccgcaatctg
     2701 gtcaccggtg aaatcaagtc ctacgcaggg gataccgtcc tgctctgcac gggcggctac
     2761 ggcaacgtgt tcttcctgtc caccaacgcg atgggctgca acgtcaccgc cgcctacaag
     2821 gcctacaagc gcggcgcttt cttcgccaac ccctgcttca cgcaaatcca ccccacctgc
     2881 attccggtca agggcgacta ccagtccaag ctgacgctga tgtcggaatc cctccgcaac
     2941 agcggccgca tctgggtgcc caagtcccgt gaaaccgcgg aaaaaatccg caaaaaggaa
     3001 atcaagcctt ccgatgttcc cgaagaagaa cgggactact acctggaacg caagtatccc
     3061 tccttcggca acctggctcc gcgcgacatt tcctcccgcg ccgccaagga agcctgcgac
     3121 gacggccgcg gcgtggccat caccggcaag ggcgttttcc tggactttgc ggatgaaatc
     3181 aagcgcatgg gccgcgaatg gattgacggc cagtacggca acctgtttga catgtacgaa
     3241 gaaatcacgg acgacgatcc ccatgaaacg cccatgatga tctaccccgc ctcccactac
     3301 accatgggcg gcctgtgggt ggactacaac ctcatgtcca acgtaaaagg gctgcacgtc
     3361 ctcggtgaag ccaacttctc cgaccacggc gccaaccgcc tgggcgcctc cgctctgatg
     3421 cagggcctgt ctgacggata cttcgtcatc cccgccacgc tgcccgccta cctgacgacc
     3481 accaagccgg gcgccgtcac caccgcttcc ccggaattca aggccgcgga agacgaggtg
     3541 aaggaacgca tcaacaaact cctgaacgtg aagggcacca agacggtgga ctccttccac
     3601 cgggaactgg gcctcatcat gtgggaagac tgcggcatgg cccgcacgga agaaagcctg
     3661 acccacgcca ttgaacgcat tccccagctc cgcaaggaat tctgggaaaa cgtgcgcgta
     3721 accggcacgg cggacggcat caacgcggaa ctggaaaaag cctgccgcgt ggccgacttc
     3781 ctggaattcg cccagctgct ctgctatgac gccctgaacc gtcaggaatc ctgcggcgcg
     3841 cacttccgcg tggaatacca gctgccggac ggcgaagcca agcgcaacga cgatgaatac
     3901 gcctacgtag ccgcctggga atacaccggc gtggacagcc tgcctgttct ccacaaggaa
     3961 ccgctgacct tcgagaacgt gcaccttgcc atccgcagct acaagtaaag atagccaata
     4021 cattaacact caaaagaaag attataacat ggctaaaaca ctcaatctca ctctcaaggt
     4081 ctggcgccag gaaaaccgtg acgccaaggg ccgcattgag acctatgccg ccaaggacat
     4141 tccggtggac gcctccttcc tggaaatgct ggacatcgtc aatgaaggtc tggttaagga
     4201 aggcaaggaa cccatccatt ttgaccacga ctgccgggaa ggcatctgcg gcatgtgctc
     4261 cctgaccatc aacggcattc cccacgggcc ggaacgccag gtaaccacct gccagcttca
     4321 catgcgtaag ttcaaggacg gcgacaccgt ctggattgaa cccttccgcg cgaaggcttt
     4381 ccccatcctg cgggacctga tggtggaccg ttccgccctg gaccgcatca tcgccgccgg
     4441 cggctacatt gacgtgcgca ccggctccgc gccgaacgcc aacaacatcg ccgtggaaaa
     4501 agtgaaggcg gacgccgcct ttgacgccgc cgcctgcatc ggctgcggcg cctgcgtggc
     4561 ctcctgcaaa aacgcctccg ccatgctgtt cacgtctgcc aagatcgctc atctcaatct
     4621 gctgccacag ggccagccgg aacgctccaa gcgcgttctg gccatgatga agcagatgga
     4681 agcggaaggc ttcggcaact gtacgaacca gtacgaatgt gaagccgcct gcccgaaagg
     4741 catcagcgtg gacaacatcg cccgcctcaa ccgcgacttc atgcgggcca acgccaagga
     4801 aggcctctgc ttctaaagta ggattccttc tcaagacgct ctacaaaaac tcccggcggc
     4861 ctcggctgcc gggagttttt tcattaccgg gtttccttga gcgaggaata aaataccaga
     4921 tagccgcttt cctggggatt tgccggcagc caggaaggaa cgaaacccta tcaagaattt
     4981 tttctaacaa atagataata ttaatgtctc ttataggaag cactgtgtgt aaagattata
     5041 atat
//
