LOCUS       c00432_gnlPro..         3361 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IFDOPIJI_432.
ACCESSION   c00432_gnlPro..
VERSION     c00432_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:30:41
            Original ID  :: gnl|Prokka|IFDOPIJI_432
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3361
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3361
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:1610]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..1610
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3361
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3361
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..875
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg432_1"
                     /sec_met_domain="Gly_radical (E-value: 4e-47, bitscore:
                     147.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-60, bitscore:
                     193.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RGEYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFE
                     MSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNN
                     DDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTP
                     FAPGANPMNGRDTKGAIAALASVAKLPFQHAHDGISYTFAVSPATLGKEREMQINNLVS
                     LLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDV
                     ISRTINHSL"
     CDS             876..1610
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg432_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             1809..2387
                     /locus_tag="ctg432_3"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPNADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             2495..3361
                     /locus_tag="ctg432_4"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGEN
                     EALELRDGDKKRYGGKGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSK
                     LGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQE
                     FMIRPVGAASFKEGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSII
                     AAIKAAGYEPGKDVMIGMDCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINE
                     YPIDSIED"
ORIGIN
        1 ttcgcggcga gtatctggaa tacgatgagg tgatggctaa gtatgagcaa atgatgcgct
       61 ggttggcgaa agtgtatgtg aacgccttga agataatcca ttatatgcat gataaatacg
      121 cgtatgaggc attcgagatg tcgcttcacg atggggatgt agagcgtatc cgtgcgacag
      181 gtatcgccgg tctttctatc gttgccgatt cgttggctgc tatccgtgat acgaaagtac
      241 gtgtgatccg tgacgagcgt ggcttggcgg ttgatttcga acgtgagggc gaatatgttc
      301 ctttcggtaa taacgatgat cgtacggata gtatcgctgt agagatcacc gagaagttca
      361 tggaatattt gcgtcagcac gagacctatc gccatgcgaa acctacgcag tccatcctta
      421 cgatcacctc taatgtggta tatggtaaga agactggtac gacaccggat ggccgtatcg
      481 gtggaactcc tttcgctccg ggcgcgaacc ctatgaacgg tcgtgatacg aaaggtgcta
      541 tcgctgcgct agcttcggta gcgaagcttc cattccagca tgcgcacgac ggcatctctt
      601 atactttcgc tgtttctccg gctacgttgg gaaaggagcg tgaaatgcag atcaataatc
      661 tggttagctt attggatggc tattttactc cggatggagg tcagcatctg aatgttaacg
      721 tatttgataa ggatttgttg atcgacgcta tggaacatcc cgagaaatat ccgcaactta
      781 cgatccgtgt atccggatat gcggtaaact tcgtgaaact gactcgtgaa caacagttgg
      841 atgttatttc aagaaccatt aaccattcac tgtaagtgat aaaaggtaag atacattcat
      901 tagagagttt cgggaccgta gatggtcccg gaattcgctt tgtcgttttt atgcagggct
      961 gcccgttgcg ttgcctgtat tgccataacc cggatacttg gaacccgaaa gggaaggtga
     1021 aatatcagat gactcccggc gagctattga cggaagtttt acggtataag agttttatcg
     1081 cccgtggggg ggtgactgtt accggtgggg aacctttatt gcaacctgag tttctgaaag
     1141 agtttttccg tttgtgccaa gagcaaggtc ttcacacagc tttggacaca tctggtttcg
     1201 tatgtacctc aaaagcttgg gaagtcttgg attatgcgga cttagtgtta ttggacatca
     1261 aaaccttaaa cccggattta catccgttac tggcgggtgt caagcaggat aatacgcttc
     1321 tgttcttgga tgaattggag agacgtggca tagatacttg gattcgccat gttattgtcc
     1381 ccggatatac ggataatgac gagtggctgg aggcgttagc tcggtatgtc agttcatata
     1441 aagtggttcg caaagtggaa ttattgcctt atcatacgat gggaacctat aaatatgagc
     1501 aattaggcct cgattatccg ttaaaggggg tggaaccctt atcaaaagaa cgtttagata
     1561 atgcgaaagc gatattctct cgctacaaac cagaaaataa caaggcctga tctctcgatc
     1621 aagccttgcg attatgcaac agctaatcag ggaaaacaat ctaatccgat tagcttttaa
     1681 acgatagtaa taattttttg cctagactaa ccttgactat ttaatatgtg tttgttttgt
     1741 tatatgcaac aaatatctat ttttgtaagg agataaaaaa tgatatagat cagaaaatga
     1801 tgaatagtat gaagacacca caagaaatag ccgaaatgat atccaaacgt ttttatccat
     1861 tacccaatgc ggatttggat tctctggcct cgatcataga atcaagtaag gctgcgaaag
     1921 gagaattatt gttggataac ggacaaatat ctaaatattt atattatgtg gaatcgggat
     1981 tattacgcca gttttactat aagaataaac gtgatattac cgagcatttc tcctgtgagg
     2041 gaaatatcgt tatttgtata gccagcacat tccaaagaga accgacgcgt ttattaatcg
     2101 aggcattgga accgacggtt tatcaccgga tctctttttc ggaatttagc gatttattga
     2161 aaacatcttt agctattaat agattgtacc agaaaatact ggagtgggct ttgatgcttt
     2221 cgcaagagaa agcggactct tggcgttttg agtcggcaag aaaacgttat ctgcgttttc
     2281 aacaggaata tccggaagca gcgaaacgtg cttctgtcaa tcatatcgcc tcgtatttat
     2341 tgatgacacc cgaatctttg agccgggtgc gggcgggagt gttgtagtct ctacctacat
     2401 tcaggtatta ttgaggtgaa aatagcaatt tgtggtgatt tcacggttca agtaatccat
     2461 ctatatttgc gtcagtttaa aaaacgtaga agcaatgaaa atagaaaaga ttacaggacg
     2521 tgagatccta gattcaagag gtaacccgac agtagaggtt gatattttac tagagtccgg
     2581 cgttatgggc cgtgcgtctg tgccttccgg tgcgtccacc ggcgagaacg aggctttgga
     2641 gttgcgtgat ggcgacaaga agcgttatgg tggtaaaggc gtattgaaag cggttgagaa
     2701 tatcaatacg attatcgctc cggcattgaa ggggatgtct gccttggatc agataggtat
     2761 cgatcatgcg atgttggctt tggacggtac gaaaactaaa tcgaaactag gtgcgaacgc
     2821 tatccttggc gtttctttag ctgtagcgaa agctgccgca aattatttgg atattccttt
     2881 atatagatat attggtggaa cgaataccta cgtgatgcct gttccgatga tgaatataat
     2941 caatggcggt tcgcatagcg acgctccgat cgctttccaa gagtttatga ttcgtccggt
     3001 aggtgccgct tctttcaagg aaggcttacg tatgggcgct gaggtattcc atgcgttgaa
     3061 aaaggtattg cacgatcgtg ggttaagtac cgctgttggt gatgagggtg gtttcgcccc
     3121 gaatttggaa ggtacggaag atgccttgaa ctcaattatc gccgctatca aggcggctgg
     3181 ttatgaaccg ggcaaggatg tgatgatcgg tatggattgc gcttcttctg agttttataa
     3241 ggatggaatc tacgattaca cgaagtttga aggagaaaaa ggagtgaaac gtacttccga
     3301 cgaacaagtg gattatctgg aaaagctgat caacgaatac ccgatcgatt ccattgagga
     3361 t
//
