LOCUS       c00334_gnlPro..         2390 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DIGDLANM_334.
ACCESSION   c00334_gnlPro..
VERSION     c00334_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 02:56:55
            Original ID  :: gnl|Prokka|DIGDLANM_334
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2390
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2390
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[777:2390]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      778..2390
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2390
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2390
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..677
                     /locus_tag="ctg334_1"
                     /transl_table=11
                     /translation="ALEPYGPMMFSGLDTHPVVLKYKFRVKAPVLRAAPPAHRVQLYLQ
                     LGGLHGLDVGKPSLFPVKKAAPAEGAPILIAPFSRLGSASEWSREQWTELVSLLPGRTV
                     LVALEEDRERAAALAEHLNVELAVGAPEALFSVMDGASVAVAVDGDFPSLCSFRGLPVV
                     TLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKDCDRDVPCNRHIAVREVLDALREISG
                     KE"
     CDS             complement(778..1245)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg334_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.3e-12, bitscore:
                     35.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VLKGCRELGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPE
                     RYRSLTGGELRPTLEFAERLASLRKPVWLRYVLVPGVTDNLEEIEGLSRHAALLGNVER
                     VDVLPFHQMGRFKWDELKLDYTLKGTPEASAKLAEQVRGIFRREGFPDCVH"
     CDS             complement(1533..2390)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg334_3"
                     /sec_met_domain="Gly_radical (E-value: 2.4e-47, bitscore:
                     147.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.2e-60, bitscore:
                     192.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMAL
                     HDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPR
                     VDDIACSLVSNFMEKLRKLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAP
                     GANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLD
                     AYFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRT
                     FHTR"
ORIGIN
        1 aagcgctgga accgtatggc cccatgatgt tctccgggct ggacacgcat cccgtcgtcc
       61 tgaaatacaa attccgggta aaggcccccg tcctgcgcgc cgcccccccg gcgcaccggg
      121 tgcagcttta tctgcaattg ggggggctgc atgggctgga cgtcggtaaa ccgtctttgt
      181 tccctgtgaa aaaagcggcc ccggcggaag gcgcccccat tttaattgct ccgttttccc
      241 gtctgggaag cgccagtgaa tggagccggg agcagtggac ggaactggtt tcccttttgc
      301 cgggacggac ggttctggtg gctctggaag aagaccggga gcgcgccgcc gcgctggcgg
      361 aacatttgaa tgtggaactg gctgtgggtg cgccggaggc tctgttttcc gtcatggacg
      421 gcgcttcggt ggcggtagcc gtggatggcg atttcccgtc cctctgttcc ttccgcgggc
      481 ttccggtggt gactctgttt tccacccggc ttccggatgt gtaccgccct atgggtacat
      541 tcaaccgttc cctttacagc caccagtgct gttccccctg cttccggaag gattgtgacc
      601 gggatgtccc ctgcaaccgc cacattgccg tgcgggaagt gctggatgct ttgcgggaaa
      661 tttccggaaa ggaataattt gggaaagggg agaagggcct ttcatccgga agatgaggag
      721 gggagatatc tgtattcggt ttgctgcggt tgatggcgcc aaggcgcgaa aatccggtta
      781 atgcacgcaa tccggaaagc cttcccgccg gaaaatgccg cggacctgtt ccgccagttt
      841 tgcggacgct tccggggtgc ccttcaaggt ataatccagc ttcaattcat cccatttgaa
      901 tctccccatc tggtggaaag ggagcacgtc cacgcgttcc acgttgccca ggagcgccgc
      961 atggcgggag agtccctcaa tttcttccag attatccgta acgccgggta ccagcacata
     1021 cctcagccac acgggcttgc ggagggaggc caggcgttcc gcaaactcca gcgtagggcg
     1081 cagttccccg cctgtcaggg agcggtaccg ttccggattc catgccttga tatccagcag
     1141 gaccaggtcc gtatccgcca gcatttcttc gcccgcattc acgcccagat ggccggaggt
     1201 gtccaggcag gtatgcagtc ccagttcccg gcagcctttc agcacggcgc cggtaaaatc
     1261 nnnnnnnnnn ctgcaaaaaa tcccggtaac gggctatttc cctgaggaca tcacccgcgc
     1321 ttctgagccg gccccgccga acgtaggagg tatccggatt gtggcagtag cggcagcgca
     1381 ggctgcagcc ggacaggaaa aggacaaagc ggatgccggg accgtccacg gtgccgcagg
     1441 attccacgga atgaaccaaa ccggtgacgg agctttctcc gtccggatca tggttttgtg
     1501 gaaaaacctg cggcatccgt gtgtcgtccg ggttagcggg tgtggaaggt gcggttgatg
     1561 acctcctgct gctgttcccg ggtcagcttg atgaaattca cggcatagcc ggaaacgcgg
     1621 atggtaagct gcgggtattt ttccgggtga tccatggcgt cgagaagcgt ttcccgttcc
     1681 agtacgttca cattaatgtg gtgacctgta gcggcaaagt aggcgtccag cagggagacg
     1741 agcttgacgc ggcgttccct ttcctccttg cccagggcct gcggaacgat ggagaaggtg
     1801 taggagatgc cgtccaggga gtcgtcatag gacagcttgg ctacggagag catggacgcc
     1861 acggctccgt tcctgtccct tccgtgcatg gggttggcgc cgggggcgaa cggttccccg
     1921 gctcggcggc cgtccggcgt gttgcccgtc tttttgccgt acaccacgtt ggaggtgatc
     1981 gtcaggatgg actgggtggg cagggaattg cggtaagtgt ggagcttgcg cagtttttcc
     2041 atgaaattgc tgaccaggct gcatgctatg tcgtccacgc gcgggtcatt gttcccgtaa
     2101 caggggaatt ccccttccgt cttgaagtcc acaatcagcc cctcttcatt gcggatggct
     2161 tttacggtgg cgtacttgat ggcggacagg gagtccgccg ccacggaaag cccggcgatt
     2221 cccgtggcca tcgtgcgcag gatttccgga tcatgcagcg ccatttcaat gcgttcatag
     2281 cagtatttgt cgtgcatgta gtggatgatg ttcagcgcgt caatgtaagt tttggcgagc
     2341 cagtcctgca tcttgtcgta cagggccatc acttcatcat agttgagaat
//
