LOCUS       c00157_gnlPro..         5267 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CIGIMPGA_157.
ACCESSION   c00157_gnlPro..
VERSION     c00157_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:28:14
            Original ID  :: gnl|Prokka|CIGIMPGA_157
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5267
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..558
                     /locus_tag="ctg157_1"
                     /transl_table=11
                     /translation="VQLYLQLGGLHGLDVGKPSLFPVKKAAPAEGAPILIAPFSRLGSA
                     SEWSREQWTELVSLLPGRTVLVALEEDRERAAALAEHLNVELAVGAPEALFSVMDGASV
                     AVAVDGDFPSLCSFRGLPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKDCDRDV
                     PCNRHIAVREVLDALREISGKE"
     protocluster    1..5267
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[658:3722]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      659..3722
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5267
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5267
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(659..1438)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_2"
                     /sec_met_domain="Radical_SAM (E-value: 4e-31, bitscore:
                     97.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSAGDVLREIARYRDFLQAAGGGVTLSGGDPLFQPDFTGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPERYRSLTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEGLSRHAALLGNVERVDVLPFHQMGRFKW
                     DELKLDYTLKGTPEASAKLAEQVRGIFRREGFPDCVH"
     CDS             complement(1455..3722)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_3"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.1e-282, bitscore:
                     927.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPKEWQGFKPGAWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDRLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRKLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             4003..4155
                     /locus_tag="ctg157_4"
                     /transl_table=11
                     /translation="MKSYACLCLLLAGVFCVACFSGACADELPPTLKPQENQRAVHDWK
                     NAMRQ"
     CDS             4155..5201
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lipase_GDSL_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg157_5"
                     /sec_met_domain="Lipase_GDSL_2 (E-value: 3e-24, bitscore:
                     74.8, seeds: 1564, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERTRTVKRGYVVFGESITLRWGVELSWGNRALGTGKAGCGVRLA
                     AVAAGFAGRAVVFFTLPAKVAPAILMHGRICLNWKPVSLFYHEFLLLHARHIAGGASVA
                     FSAPSIPPSALPATLKPEPHQRDPYDWNARHEAVKKRNRTVKPEYVMIGDSITHHWGGE
                     PSGDGKNTGAGSWEKLFRPHTATNMGFGFDYVDNAYYRVQNGELDGISPRVIIVLLGTN
                     NLGHRKDTPQACADNIRAFVRLVRRKCPSSKILLLGVLPRKEKNLAEPVKQTNRLLAEL
                     HNGKNIFFADPGKALLSADGASPQNEFMKDVVHPNARGYEVLEKELAVLLKKLDAKYRG
                     GKQAGKHS"
ORIGIN
        1 gtgcagcttt atctgcaatt gggggggctg catgggctgg acgtcggtaa accgtctttg
       61 ttccctgtga aaaaagcggc cccggcggaa ggcgccccca ttttaattgc tccgttttcc
      121 cgtctgggaa gcgccagtga atggagccgg gagcagtgga cggaactggt ttcccttttg
      181 ccgggacgga cggttctggt ggctctggaa gaagaccggg agcgcgccgc cgcgctggcg
      241 gaacatttga atgtggaact ggctgtgggt gcgccggagg ctctgttttc cgtcatggac
      301 ggcgcttcgg tggcggtagc cgtggatggc gatttcccgt ccctctgttc cttccgcggg
      361 cttccggtgg tgactctgtt ttccacccgg cttccggatg tgtaccgccc tatgggtaca
      421 ttcaaccgtt ccctttacag ccaccagtgc tgttccccct gcttccggaa ggattgtgac
      481 cgggatgtcc cctgcaaccg ccacattgcc gtgcgggaag tgctggatgc tttgcgggaa
      541 atttccggaa aggaataatt tgggaaaggg gagaagggcc tttcatccgg aagatgagga
      601 ggggagatat ctgtattcgg tttgctgcgg ttgatggcgc caaggcgcga aaatccggtt
      661 aatgcacgca atccggaaag ccttcccgcc ggaaaatgcc gcggacctgt tccgccagtt
      721 ttgcggacgc ttccggggtg cccttcaagg tataatccag cttcaattca tcccatttga
      781 atctccccat ctggtggaaa gggagcacgt ccacgcgttc cacgttgccc aggagcgccg
      841 catggcggga gagtccctca atttcttcca gattatccgt aacgccgggt accagcacat
      901 acctcagcca cacgggcttg cggagggagg ccaggcgttc cgcaaactcc agcgtagggc
      961 gcagttcccc gcctgtcagg gagcggtacc gttccggatt ccatgccttg atatccagca
     1021 ggaccaggtc cgtatccgcc agcatttctt cgcccgcatt cacgcccaga tggccggagg
     1081 tgtccaggca ggtatgcagt cccagtttcc ggcagccttt cagcacggcg ccggtaaaat
     1141 caggttggaa gagggggtcc ccgccggaga gggtgacgcc gccgcccgcc gcctgcaaaa
     1201 aatcccggta acgggctatt tccctgagga catcacccgc gcttctgagc cggccccgcc
     1261 gaacgtagga ggtatccgga ttgtggcagt agcggcagcg caggctgcag ccggacagga
     1321 aaaggacaaa gcggatgccg ggaccgtcca cggtgccgca ggattccacg gaatgaacca
     1381 aaccggtgac ggagctttct ccgtccggat catggttttg tggaaaaacc tgcggcatcc
     1441 gtgtgtcgtc cgggttagcg ggtgtggaag gtgcggttga tgacctcctg ctgctgttcc
     1501 cgggtcagct tgatgaaatt cacggcatag ccggaaacgc ggatggtaag ctgcgggtat
     1561 ttttccgggt gatccatggc gtcgagaagc gtttcccgtt ccagtacgtt cacattaatg
     1621 tggtgacctg tagcggcaaa gtaggcgtcc agcagggaga cgagcttgac gcggcgttcc
     1681 ctttcctcct tgcccagggc ctgcggaacg atggagaagg tgtaggagat gccgtccagg
     1741 gagtcgtcat aggacagctt ggctacggag agcatggacg ccacggctcc gttcctgtcc
     1801 cttccgtgca tggggttggc gccgggggcg aacggttccc cggctcggcg gccgtccggc
     1861 gtgttgcccg tctttttgcc gtacaccacg ttggaggtga tcgtcaggat ggactgggtg
     1921 ggcagggaat tgcggtaagt gtggagcttg cgcagttttt ccatgaaatt gctgaccagg
     1981 ctgcatgcta tgtcgtccac gcgcgggtca ttgttcccgt aacaggggaa ttccccttcc
     2041 gtcttgaagt ccacaatcag cccctcttca ttgcggatgg cttttacggt ggcgtacttg
     2101 atggcggaca gggagtccgc cgccacggaa agcccggcga ttcccgtggc catcgtgcgc
     2161 aggatttccg gatcatgcag cgccatttca atgcgttcat agcagtattt gtcgtgcatg
     2221 tagtggatga tgttcagcgc gtcaatgtaa gttttggcga gccagtcctg catcttgtcg
     2281 tacagggcca tcacttcatc atagttgaga atttcttccg tgtagcgggg ggagggcggg
     2341 gccacctgtt tgcccttgag ttcatccacg ccgccgttca gggcgtagag aaggcacttg
     2401 gcgaggttgg cgcgcgctcc gaagaactgc atctgcttgc cgatgcgcat ggcggacacg
     2461 cagcatgcga tgccgtagtc gtcgccccag tgcgggcgca tcaggtcgtc gttctcgtat
     2521 tggacggagg acgtttcaat ggaaaccttg gcgcagaagc ttttgaaggc ttccggcagg
     2581 ttccgggacc acagaacggt caggttcggt tccggagccg gccccaggtt gtacagggtc
     2641 tgcagcatgc ggaaggaact gcgggtgacg agggtgcgcc cgtcctctcc catgccgccg
     2701 atggattccg tcacccaggt ggggtctccg gagaacaggt tattgtaatc aggcgtgcgg
     2761 atgaaacgga cgatgcgcag cttcatcacg aactggtcga tgatttcctg aacttcctct
     2821 tccgtgatga gcccctgttc caggtcgcgg gtgaaataaa tgtcgaagaa agtggaaacg
     2881 cggcccaggg acatggccgc tccgttctgt tccttcacgg cggccaggta gcccaggtac
     2941 gtccattgca cggcttcgcg ggcgtttgcg gcggggcgag tgaggtcgca gccgtaggag
     3001 gcgcccagct gggcgagttc ttccagggcg cgtatttgtt cgctcatttc ttcgcgcagg
     3061 cggatcagct catccgtaag gggggaatgt tcacggtttt tgaggtcctt gcggcgttcg
     3121 gcaatcagtc tgtccgtgcc gtacagggcc acccggcggt agtccccgat gatgcggccg
     3181 cggccatagg cgtccggaag gccggtaata atgccggcgg aacgggccgc ccggatatcg
     3241 gaagtgtagg cgtcgaaaac gccttcattg tgcgtcttgc gtatcttttt gaaaatatcc
     3301 gcggttttct cgcacatctt gaacccgtag gattccaggg cctgctgggc catgcgcagg
     3361 ccgccaaagg gcatcagggc ccgtttcagg ggggcgtccg tctggaggcc tacgaccact
     3421 tccagttcct tgtcaatgta gccgggcttg tgggaggtga tggaggatac cacttcttca
     3481 tcagcatcca gaacgccgcc gttgtctatt tcccttttca gcaggacttt cagttcatcc
     3541 cataaacgga gcgtgcgctg ggaggggccg gaaagaaatt cttcattgcc ggaatagggc
     3601 gtgtagttat gctggatgaa atcccgtacg tcaatggact ccgtccatgc tcccggcttg
     3661 aatccctgcc attccttcgg aagggcggtg gactccttca agtcttttac tatgctgatc
     3721 ataaatagga tgtagtgaag aagaccggag aaaaacgttc cgcagccgtt gaaaggccgg
     3781 aaacggcaaa actccgcgcc acgtaattat atcgtgaatt ccgggagaca acaaggctaa
     3841 ttctttgcga agagaacgcg ggtgcgctgg ctttggcatg tttcacctgt ctgtccgttg
     3901 cggagcggaa aatgagggga acatgcgatt gcggaaaagt gacggaagat tatttctctt
     3961 tactttccta atctctattg cagtatctca acgcttaccg ttatgaaatc ttatgcatgc
     4021 ctttgtttat tgctggcggg agttttttgc gttgcttgtt tctccggtgc ctgtgctgat
     4081 gaattgccgc ccacgctgaa gccccaggag aaccagcggg ccgtgcatga ctggaaaaac
     4141 gccatgaggc agtgatggaa cggacccgga ccgtcaaacg ggggtatgtc gtttttggtg
     4201 aaagcattac cctccgttgg ggagtcgagc tttcctgggg caacagggct ctcggaacag
     4261 gaaaggccgg atgcggtgtt cgcctggccg ctgttgcggc gggatttgcc ggacgggcag
     4321 tggtcttttt tactctgccg gcaaaagtgg cgcctgcgat cttgatgcac ggcaggattt
     4381 gcttaaactg gaaaccggtt tcactttttt atcatgaatt tcttctccta catgctcgcc
     4441 atattgctgg gggggccagt gtggcgtttt ccgcaccttc catccctccc tccgcgttgc
     4501 ccgcaaccct gaagccggag ccccatcagc gggatccgta tgattggaat gcccggcatg
     4561 aagccgttaa aaaaagaaac cggaccgtca agcctgaata tgtgatgata ggggacagca
     4621 ttacccacca ctggggaggg gagccttccg gggacggaaa aaacacggga gcgggttcct
     4681 gggagaaatt attccgtccg catactgcga cgaatatggg ctttggattt gattatgtgg
     4741 ataatgccta ttaccgtgtg caaaacgggg agctggacgg catttctccg cgggtcatca
     4801 tcgtgctgct gggaaccaat aatctggggc accgcaagga tacgccgcag gcatgtgcgg
     4861 acaatatcag ggcgtttgtc cgcctggtgc gccggaaatg cccctcttcc aaaattctgt
     4921 tgctgggcgt tcttccaagg aaggagaaaa acctggcgga accggtgaag cagacgaaca
     4981 ggctgctggc ggagctgcat aacggaaaga acattttctt tgccgatccg ggaaaggctc
     5041 ttctttccgc agacggtgct tctcctcaga atgaatttat gaaggatgtg gtgcatccca
     5101 atgccagggg atatgaggtt ttggaaaagg aactggccgt acttttgaaa aagcttgatg
     5161 caaaataccg gggagggaag caggcgggaa agcattcctg atgatcgtct tctgatggaa
     5221 ggcaggattt tgcttgaagg gaaagccccg gctgaggaaa atatacg
//
