LOCUS       c00224_gnlPro..         3658 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CIGIMPGA_224.
ACCESSION   c00224_gnlPro..
VERSION     c00224_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:28:14
            Original ID  :: gnl|Prokka|CIGIMPGA_224
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3658
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3658
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[65:3555]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      66..3555
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3658
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3658
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             66..767
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg224_1"
                     /sec_met_domain="SucD_FumR (E-value: 1.2e-14, bitscore:
                     42.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             789..2747
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg224_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 2e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 5.7e-39,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             2788..3555
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg224_3"
                     /sec_met_domain="Fer2_3 (E-value: 1.1e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 3.6e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIASGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 gaggcttgcc atccgggctt gaaccgggca tcctgtgaag acgcatttat ttcattagag
       61 cgcctatgaa tgcactagta aaaaccatat gtacattcgt gacatcctcc atcggccgca
      121 aaatcattgt ggctctgacg gggttatgtt tggtcttgtt ccttgcggga cacctggcag
      181 gcaaccttct catcttcgga ggccctgaat ggatcaacac ctacgcccac ggcctgcact
      241 ccatgccgga agcagccctc tggggaatcc gcgcggggct tgcggtgatc ttcatcatcc
      301 acgtctggct gaccatccag ctgaagctgg aaaaccatgc ggcccgcgaa ccctacgtgt
      361 tcaagaacac cattaaggca acgctctcct cccgctacat gatttacacc ggtttgaccg
      421 tcctggtgtt cctgatctac cacctgtacc agtacaccct gcgcgtggga tacgaccccg
      481 cccagttcac cacctttatt tccgacggta cggtggaaac ctttgacgtg tacaagatga
      541 tcgtgaccgg attctccaac gtatggtgct ccgccttcta catcctggcc gttctgatgc
      601 tgttcagcca tctgcgccac ggggtacagt ccatcttcca gactgtgggc gtcgattccc
      661 gcaaaatccg gcccttctac aactttatcg ccatcgccta cggcgcgatt atctgcctgg
      721 gcttcatctc cgtgccggtc tcggttcttc tgggtatcat caaataacca tcaactcctc
      781 tcaaagtcat gattaatttt cccgtaagcg actggatgcc ggatgccaaa attccctccg
      841 gccctattca ggacaaatgg tccaagtaca aacttgagtc caaactgatc aatccgaaca
      901 acaagcgcaa gtacacggta cttgtggtag gttccggcct ggctggcgcc tccgctgccg
      961 ccacgcttgc cgaacttggc tacaacgtca aatgcttctg ctaccaggac agcccccgcc
     1021 gcgcccactc cattgcggcc cagggcggca ttaacgcggc caagaactac cagaacgacg
     1081 gcgactccgt ttaccgcctg ttttacgaca ccgttaaagg cggcgacttc cgcgcccgtg
     1141 aagccaatgt ctaccgtctg gcggaagtct cctccaacat cattgaccag tgtgtggccc
     1201 agggcgttcc cttcggccgt gaatacggtg gcctgctgga caaccgctcc ttcggcggcg
     1261 cccagctcaa gcgaaccttc tacgcacgcg gacagacggg ccagcagctc ctgctgggtg
     1321 cctaccaggc cctggaaaag gaagtggcca agggacacat tgaaatgttc tcccgccatg
     1381 aaatgatgga cctggtgttg gtggacggcg aagccaaggg catcgtcacc cgcaatctgg
     1441 ttaccggcga aatcaaatcc tacgcagggg ataccgtcct gctctgcacg ggcggctacg
     1501 gcaacgtgtt cttcctgtcc accaacgcga tgggttgcaa cgtcactgcc gcctacaaag
     1561 cctacaagcg tggtgccttc ttcgccaacc cctgcttcac gcaaatccac cccacctgca
     1621 ttccggtcaa aggcgactac cagtccaaac tgacgctgat gtcggaatcc ctccgcaaca
     1681 gcggccgcat ctgggtgccc aagtcccgtg aaaccgcaga aaaaatccgt aagaaggaaa
     1741 tcaagccttc cgatgttccc gaagaagaac gggactacta cctggaacgc aagtatccct
     1801 ccttcggcaa cctggctccg cgcgacattt cctcccgcgc cgccaaggaa gcctgcgacg
     1861 acggccgcgg cgtggccgtc accggcaagg gcgtcttcct ggattttgcg gatgaaatca
     1921 agcgcatggg ccgcgaatgg attgacggcc aatacggcaa tctgtttgac atgtacgaag
     1981 aaatcacgga cgacgacccc catgaaacgc ccatgatgat ctaccccgcc tcccactaca
     2041 ccatgggcgg cctgtgggtg gactacaacc tcatgtccaa cgtgaagggg ttgcacgtcc
     2101 tcggtgaagc caacttctcc gatcacggcg ccaaccgcct gggagcctcc gccctgatgc
     2161 agggcctgtc tgacggctac ttcgtcattc ccgccacgct gcccgcttac ctgacgacca
     2221 ccaagcccgg tgccgtcacc accgccgccc cggaattcaa ggctgcggaa gacgaagtga
     2281 aggaacgcat caacaagctc ctgaatgtga agggcaccaa gacggtggac tccttccacc
     2341 gggaactggg cctcatcatg tgggaagact gcggcatggc ccgcacggaa gaaagcctga
     2401 cccacgccat tgaacgcatt ccccagctcc gcaaggaatt ctgggaaaac gtgcgcgtaa
     2461 ccggcacggc ggacggcatc aacgcggaac tggaaaaagc ctgccgcgtg gccgacttcc
     2521 tggaattcgc ccagctgctc tgctatgacg ccctgaaccg ccaggaatcc tgcggcgccc
     2581 acttccgcgt ggaataccag ctgccggacg gcgaagccaa gcgcaacgac gatgaatacg
     2641 cctatgtagc cgcctgggaa tacactggcg tggacagcct gcctgttctc cacaaggaac
     2701 cgctgacctt cgagaacgtg caccttgcca tccgcagcta caagtaaaga tagccaatac
     2761 attaacactc aaaagaaaga ttataacatg gctaaaacac tcaatctcac tctcaaggtc
     2821 tggcgccagg aaaaccggga agctaagggc cgcattgaga cctatgccgc caaggacatt
     2881 ccggtggacg cctccttcct ggaaatgctg gacatcgtca atgaaggcct ggtcaaggaa
     2941 ggcaaggaac ccatccattt tgaccacgac tgccgggaag gcatctgcgg catgtgctcc
     3001 ctgaccatca acggcattcc ccacgggccg gaacgccagg tgaccacttg ccagcttcac
     3061 atgcgcaagt tcaaggacgg cgataccgtc tggattgaac ccttccgcgc gaaggctttc
     3121 ccaatcctgc gggacctgat ggtggaccgc tccgccctgg accgcatcat cgcctccgga
     3181 ggctatattg acgtgcgtac cggttccgcg ccgaacgcca acaacatcgc cgtggaaaaa
     3241 gtgaaggcgg acgccgcctt tgacgcggcc gcctgcatcg gctgcggcgc ctgcgtggcc
     3301 tcctgtaaaa acgcctccgc catgctgttc acctccgcca agatcgccca tctcaatctg
     3361 ctgccgcagg gccagccgga acgttccaag cgcgtcctgg ccatgatgaa gcaaatggaa
     3421 gcggaaggct tcggcaactg cacgaaccag tatgaatgtg aagctgcctg cccgaaaggc
     3481 atcagcgtgg acaacatcgc ccgcctcaac cgcgacttca tgcgagccaa cgccaaggaa
     3541 ggtctctgtt tctaaagaag attccttctt aagacgctct gtaaaaactc ccggcggcat
     3601 cggctgtcgg gagttttttt cattatcagg tttccttgaa ccaggaataa aatatcag
//
