LOCUS       c00275_gnlPro..         4764 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HOMOEMGA_275.
ACCESSION   c00275_gnlPro..
VERSION     c00275_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:09:35
            Original ID  :: gnl|Prokka|HOMOEMGA_275
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4764
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..306)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg275_1"
                     /sec_met_domain="ECH_1 (E-value: 8.6e-21, bitscore: 63.2,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWKQIKEYEEILFDFYNGIARITINRERYRNAFTPTTTTE
                     MSDALYICRERPDICVIVLTGAGDTAFCAGGDMNVKGHGGYIGKDGVARLNVLDVQK"
     protocluster    1..4764
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:4385]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..4385
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..4764
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4764
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             complement(333..1358)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg275_2"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.6e-08, bitscore:
                     22.7, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIIVGQMSLIYLFEKKISKLLYKHFAWLTEHTSNKTIPGIPVKNF
                     DAAIYALPEETQEKMVPELVITYGGHIVSKRLKKFLRNNPPKEHWHVSADGEVADLYGA
                     MTTVIEMDPFEFLEKIAYLLENKPTEFPRVWENNTKSLPEPEFAYSEMAAIGRLLKSLP
                     TPSALHLGNSSTVRYAQLFTLPEEVEVCCNRGTSGIEGSLSTAIGYAVASDKLNFVVIG
                     DLSFFYDMNALWNSNFGCNLRILLLNNGGGEIFQALPGLSMSGKTHRYVTATHKTSAQG
                     WATERGFLYQGVHDTAELEEAMKVFTDPNPINQPVLMEVFTDKDEDVRLLKEYYHSLKT
                     L"
     CDS             complement(1418..2011)
                     /locus_tag="ctg275_3"
                     /transl_table=11
                     /translation="MYTDKKNILQLVALLKEHSISKIVLCPGSRNSPIVHTLANHPFFT
                     CYSVTDERSAGFFAMGLALHGGKPAAVCCTSGTALLNIHPAVAEAYYQHVPLIVISADR
                     PGAWIGQMDGQTLPQPGVFGSLVKKSVNLPEIHTDEDEWYCNRLVNXXXXXXXXXXXXM
                     EKDRFISMCRSPNLCSSSLLPNCRKCALSPAIRD"
     CDS             complement(2077..2457)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg275_4"
                     /sec_met_domain="Chorismate_bind (E-value: 3.4e-37,
                     bitscore: 117.2, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGPYSARAGALCHLKSDFRFRLDSNDKLGSLLKLLHPTPAVCGL
                     PKDDAYRFILENEGYDRRYYSGFIGWLNPKGRTDLYVNLRCMNINKKTQTLYAGGGLLS
                     SSELSDEWQETEDKLQTMRALL"
     CDS             complement(2944..3156)
                     /locus_tag="ctg275_5"
                     /transl_table=11
                     /translation="MIDSLIRQGRSFAIYRIPGEEEPRFVMQASGSARLLYNIEDLNEQ
                     XXXXLRHRSVPGQPGMSHRADSARL"
     CDS             complement(3153..4385)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg275_6"
                     /sec_met_domain="HBT (E-value: 1.7e-17, bitscore: 52.6,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNSEKAITARTQAALLKVQQMGIHIVLASGR
                     PTNGVMPLAKALELDHYGGFILSYNGGQIINVQTGELLFEKRINPEMLPYLDKKAKKND
                     FAIFTYHKDYILTNKPDNKHVQEEAALNNMRVIGVDNFPEAVDFSPCKCVLASDNEEEL
                     VGLENHWKKRLDGVLDVFRSEDFYLEVVPQSINKGNTLGVLMEKLKITTEEVVAIGDGI
                     ADVPMLQLAGTSVAMGNARDSVKVCTDFTTLTNDQDGVAIAVETAILAAIKPTEVPLDQ
                     LNIRAKHALMGNLGIQYTYASEDRVEATMPVDERTRQPFGILHGGATLALAETVAGLGS
                     MILAKPDEIVVGMQVSGSHMSSAHEGDTVRAVGTIIHKGRSSHVWNVDVFTSTDKLVSS
                     IRVVNSILKKK"
     CDS             complement(4482..4763)
                     /locus_tag="ctg275_7"
                     /transl_table=11
                     /translation="EVVPKGIDKAASLERLLERVQIKREEVIAIGDGYNDLSMIKFAGL
                     GVAMANAQPPVKSNADRITAFTNDEDGVAHFIHDLLNSNTHQNIHEIG"
ORIGIN
        1 tttctgtacg tcgagtacat tcaaacgggc aacgccgtcc ttaccgatat acccgccatg
       61 acctttcaca ttcatatctc caccggcaca gaaagcggtg tcgccagctc cggtcaagac
      121 gatgacacaa atatccggac gctcccggca aatgtaaagc gcatcgctca tttcggttgt
      181 cgtggtggga gtgaaggcat tacggtaacg ttcgcggttg atagtgatac gggcaatacc
      241 attgtagaaa tcaaacaaga tttcttcata ttcttttatc tgtttccatt ctctttgtgt
      301 tgacataaaa tctttattta gagtattgtt tattataacg tctttaaact atggtagtat
      361 tctttcagca ggcggacgtc ttcatctttg tcggtgaata cctccatcaa tacgggttga
      421 ttgatcggat tcggatcggt gaacactttc atggcttctt ccaattctgc cgtgtcgtgt
      481 acgccctggt agaggaatcc ccgttcggta gcccagccct gggccgaagt cttatgtgta
      541 gccgttacat agcggtgtgt ctttcctgac atcgacaaac cgggcagtgc ctggaagatt
      601 tcgccgccac cgttattcag taacaggatg cgcaggttac agccgaagtt gctgttccaa
      661 agcgcgttca tatcataaaa gaagctcaga tcgcctatca ccacaaaatt caatttgtcg
      721 gatgctaccg catagccgat agccgtggat aatgaacctt ctataccgct tgttccccgg
      781 ttgcagcaaa cttcaacttc ttcgggcaac gtaaacaatt gagcgtatcg taccgtcgaa
      841 ctattgccta aatgtaaggc agacggagtc ggcaatgact tgagcaaacg accgattgct
      901 gccatttccg aataagcaaa ctccggttcc ggcagactct tcgtattgtt ttcccagaca
      961 cgtggaaatt ctgtcggctt attttccagc agataagcaa tcttttcaag aaactcgaac
     1021 gggtccattt ctattacggt agtcattgca ccgtataagt cagccacttc accatcggcc
     1081 gatacgtgcc aatgttcttt cggcggatta ttacgtagga atttctttaa acgtttggat
     1141 acgatgtgtc cgccgtaggt aataaccagt tcgggaacca tcttttcctg cgtttcttca
     1201 ggcagggcat aaatggctgc atcgaagttt ttgaccggga tgcccggaat ggtcttgttg
     1261 cttgtatgtt cggtcagcca ggcgaaatgt ttataaagta gcttggatat cttcttttca
     1321 aaaagataaa tcaggctcat ctggccgaca atgatcattc gtttattata gctgttcaac
     1381 cgttcgatca gggtattgta ttccctgtcg tacacattca gtccctgata gcgggtgata
     1441 acgcgcactt ccggcaattc gggagtagtg aactggaaca aaggttcgga gatcggcaca
     1501 ttgatatgaa ccggtccttt tccatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 ngttgacaag acggttgcaa taccattcat cttcatccgt atgaatttcc ggcagattga
     1621 ccgatttctt aaccagggaa ccgaatacgc ccggttgcgg taaagtctgt ccgtccattt
     1681 ggcctatcca ggctcccgga cggtctgccg atatgacgat aagaggaaca tgctggtagt
     1741 aggcttccgc cacagccgga tggatattca gcaaagccgt acccgaagta cagcaaacgg
     1801 ctgccggttt tcctccgtgc aatgccagac ccatggcaaa gaatccggca cttcgttcgt
     1861 cggtgacgga atagcaggtg aagaacggat gattcgccaa tgtatgtacg ataggcgaat
     1921 tacggcttcc cggacataat acgattttac ttatactatg ctccttgagc aaggccacta
     1981 actgaagaat gtttttctta tcggtataca tatattatat ttatcttatt ctgcttacaa
     2041 aagtacatat aatatctaac gcaaaacgcc tgtctatcat aataaggccc gcatcgtttg
     2101 cagtttgtct tccgtttcct gccattcgtc gcttaattct gaagaggaga gcaatcctcc
     2161 tccggcatag agtgtctgtg tcttcttatt gatattcatg cagcgcagat ttacatacag
     2221 gtcggttctg cctttcgggt tcaaccaacc tataaaaccg gaatagtaac ggcggtcgta
     2281 accttcattt tccaggataa aacggtaggc gtcatctttg ggcaatccac aaacggcagg
     2341 ggtagggtgt agcagtttca acaaactacc cagcttatcg ttactgtcca gccggaacct
     2401 gaaatcactt ttcagatgac ataaagcccc ggcacgtgcc gaatagggac cttccatcgt
     2461 cgattcgata cccagtgaac ttagctggtc gtggatataa ttggagacta gttgttgttc
     2521 ccttttgttt ttctcatccc aataagtcgg cagttcgccg ttctgtaaag gctgtgttcc
     2581 tgccagcgca accgtatgcc attcgccctt ttctcccgag aggataattt ctggggttcc
     2641 tcacagccag atgcccgttt nnnnnnnnnn ggtgtatggc agaggtaaac ataagaacgg
     2701 atatactgtt tgcaggcctt ataaaaagcc tttgcaggcg aaaagccgga ttccctgtcg
     2761 agtgtcagac tgcgcgaaag taccagtttg tccagctgtc tctcctgcaa aggcttgata
     2821 aaggaatcga aacaatgtgt atagttgtct tttgtcttcc gttcggatgg gttttctgtt
     2881 ccattcggat gaaactccaa atccattcgg atgtaatccg gttgactgtt atccggcaat
     2941 tcgctataat cgcgccgaat cagcacgatg ggacattccc ggctgacccg gaacggagcg
     3001 atgacgaagc nnnnnnnnnn gttgttcatt caggtcctcg atgttatata aaagacgggc
     3061 agaaccggag gcttgcatca cgaaccgggg ttcttcttcc cccgggatac gatagattgc
     3121 aaaactccgg ccctgcctga tcagtgagtc tatcatttct tttttaatat actatttacg
     3181 acacgtatgg atgataccaa tttatcagta gaggtaaaca catctacatt ccatacatgt
     3241 gaagaacgtc ccttgtgaat aattgtcccg acggcacgta ccgtatcgcc ttcgtgggcc
     3301 gacgacatat ggctgccgct cacctgcatt ccgactacaa tctcgtccgg cttagccagt
     3361 atcatggagc ctaatcccgc aactgtttca gccaatgcca gagtcgctcc gccatgcagt
     3421 atgccgaaag gctgtcgtgt acgttcgtcg accggcattg tcgcttccac acggtcttcg
     3481 gatgcataag tatattgaat acccaagtta cccatcagtg catgtttagc ccgtatattc
     3541 aattggtcca aaggaacttc tgtcggtttg atggcagcaa ggatagcggt ttctacggct
     3601 attgccacac catcctgatc gttggtcagt gtggtgaaat ccgtacagac cttaaccgaa
     3661 tcacgggcgt tgcccatcgc tacgctggtc ccggcaagct gtaacatggg gacatcagca
     3721 attccatcgc cgatcgctac gacttcttcg gtcgtaattt tcagcttttc catcaatacg
     3781 cctaaagtgt ttcctttgtt gatggattgg ggaaccacct ccagataaaa atcttccgaa
     3841 cggaatacgt ccaatacacc gtccagccgt ttcttccagt gattttccaa tcctaccagt
     3901 tcttcttcgt tatcgcttgc cagcacacac ttgcaaggac tgaagtcgac tgcctccggg
     3961 aaattatcaa caccgatcac acgcatattg tttagcgcgg cttcttcctg cacatgtttg
     4021 ttgtcgggtt tatttgtcag gatatagtct ttatgatatg tgaaaatggc aaaatcgttt
     4081 ttcttcgctt tcttatccaa gtaaggcagc atctccgggt tgatacgttt ctcgaagagc
     4141 agttcacccg tttgtacgtt gatgatctgg ccaccgttat atgagagaat gaaaccaccg
     4201 taatgatcca gttccagagc ctttgcgagc ggcataaccc cgttggtggg tctgccggat
     4261 gccaatacaa tgtgtattcc catttgctga actttcagta aagcagcctg ggtacgggca
     4321 gtaattgctt tttcactgtt gagcaatgtc ccgtctacgt ctaaaaccaa taatttgtac
     4381 ttcatggctt gctgtttttt tgtttagatt acaaatctac ataaaaatcc tggtttataa
     4441 acgcatccaa taaaaataaa gaccatcttg ttagtatctt tttaacctat ttcatgtata
     4501 ttttggtggg tattactatt gagtaagtca tgaatgaaat gtgcaacgcc atcctcatca
     4561 ttcgtaaatg cagtgatccg atctgcattt gattttacgg gaggctgtgc atttgccata
     4621 gcaacgccga gtccggcaaa tttgatcatg gaaaggtcat tgtagccgtc accgatggcg
     4681 ataacttcct ctctttttat ctgcacacgt tccaataggc gttccagcga agcggctttg
     4741 tcgatccctt tgggcacgac ttcc
//
