LOCUS       c00168_gnlPro..         5225 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ANJMKACL_168.
ACCESSION   c00168_gnlPro..
VERSION     c00168_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:45:09
            Original ID  :: gnl|Prokka|ANJMKACL_168
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5225
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5225
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2487:5225]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2488..5225
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5225
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5225
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..956
                     /locus_tag="ctg168_1"
                     /transl_table=11
                     /translation="SFQAFAVSLASRVGTGNLAGVATAIVVGGPGAVFWMWVIALVGAA
                     SAFVESTLAQLYKRRGRDSFIGGPAYYMERGLGRRWMGVLFAVLISVTFGFAFNSVQSN
                     TICAAWEGAFGADHVWVGVVLTALTLLIIFGGIRRIARVSGVIVPIMALGYIVLALGVV
                     LFNLGRLPEVLELIVADAFGWEQTLGGAVGAALMQGIKRGLFSNEAGMGSAPNVAATAH
                     VSHPVKQGLIQTLGVFTDTLVICTCTAFIILFGGVPDASLNGIQLTQAALESEIGPAGG
                     GGSSPWRSSCSPFRRSSAIITTAKPTSASSLRGRGR"
     CDS             complement(1212..2366)
                     /locus_tag="ctg168_2"
                     /transl_table=11
                     /translation="MKNRLPDLNVPARERIGWVDLLRVIACFLVVFSHSCDAFVAVFDS
                     DRATFLQGALAGSFVRACVPLFVMMSGVLLLPVRTGTGAFYRKRIGRVLLALVFWSLTL
                     PVLYYLYMRYVGTSSPSIAPALFTGEATLHKMWTFVFNFCYDTTPLWYLYMLIGLYLIM
                     PLISPWLERASRRELQSVLAIWGVTLLLPYVKMLAPALGYMGNYGNTGLYGVCDWNEFG
                     TFHYVSGFAGYLVLAFYLVKFPPAWNWRRTLGICIPTFLAGYLATGLGYVVMQKHFPGN
                     YAYLEIVWYFAGINVFMMTAPVFILVQKAAARPRAWLSRLAGATFGIYLCHFIFVQAGY
                     DLVQRIPGLPALARIALIACGAFAVSWAVVRLMQRWSVTRRLVE"
     CDS             complement(2488..3213)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 69.5;
                     E-value: 1.2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg168_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-28, bitscore:
                     88.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAGAG
                     TLTDPAEILRMAVDQKPFFGRRGGVTFSGGEPTFQAAELVPLVKSLKEAGIHVCIDSNG
                     GVWNPAVEELLGLVDLVLLDVKQADPERHRALTGRENAQTLRTAAWLEAHGKPFWLRYV
                     LVPGISDAEADIRALGERLGGYKSVERVELLPYHTLGVHKYEAMGLEYKLRDVRENTPE
                     QLDRAAALLREYFPTVVVN"
     CDS             complement(3309..5225)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg168_4"
                     /sec_met_domain="Gly_radical (E-value: 5.5e-47, bitscore:
                     147.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1016.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FGGINVVSRACKENGVEVDEKVKDIFTHYRKTHNDGVFDVYTEEI
                     RSFRSLGFLTGLPDNYARGRIIGDYRRLALYGTDRLIEAKQEDLRNLTGPMTDARIRLR
                     EEVAEQIKALKDIRTMGEYYGLDLSRPAHSAQEAVQWVYMAYLAAVKEQDGAAMSLGNV
                     SSFLDIFIEYDLAHGTIDESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAI
                     GGRFNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTILWSPELPQGFKDFCAKVSADTSSI
                     QYENDDLMREVRHSDDYGIACCVSFQDIGRQIQFFGARTNLAKALLLAINEGRCENTGT
                     LMVKGIPALSEGPLRFEEVMRNYKMVLKEIARVYNEAMNIIHYMHDKYYYEKAQMAFVD
                     TDPRINLAYGVAGLSIALDSLSAIKYARVTPRRNAEGLTEAFDIEGEFPCYGNNDDRVD
                     HLGVDLVYYFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRLKGVAFAPGA
                     NPMHGRDKSGAIASLASVAKMRYRDSQDGISNTFSIVPKSLGPTAGERIENLVTMLDGY
                     FTKGAHHLNVNVLNREMLEDAMEHPEKYPQLTIRVSGYAVNFTKLSREHQLEVISRSFH
                     ERM"
ORIGIN
        1 cgtcgttcca ggctttcgcc gtgtcgctgg ccagccgcgt cggcacgggg aatctcgcgg
       61 gcgtcgccac ggccatcgtc gtcgggggac ccggcgcggt cttctggatg tgggtcatcg
      121 cccttgtggg cgccgcgagc gctttcgtcg agtcgaccct cgcacagctt tacaaacgcc
      181 gcggcaggga ctctttcatc ggcggtccgg cctactacat ggaacgcggc ctcgggcgga
      241 ggtggatggg cgtgctgttc gccgtgctga tctccgtgac cttcggattc gccttcaact
      301 cggtgcagag caacacgatc tgcgccgcgt gggagggagc tttcggcgcc gatcatgtgt
      361 gggtgggcgt cgtgctcacg gcgctgacgc tgctcatcat cttcggaggc atacggcgca
      421 ttgcgcgtgt cagcggcgtg atcgtcccga ttatggcgct ggggtacatc gtgctggcgc
      481 tgggcgtcgt gctgttcaac ctcgggcggc tgcccgaagt gctggaactg atcgtcgccg
      541 acgctttcgg ctgggagcag acgctgggcg gcgcggtcgg cgcggcgttg atgcagggca
      601 tcaagcgggg acttttcagc aacgaggccg gtatgggttc ggccccgaac gtcgccgcca
      661 cggcccacgt ttcgcacccc gtgaagcagg gacttatcca gaccctcggg gtcttcaccg
      721 atacgctcgt catctgcacc tgcacggcct tcatcatcct gttcgggggc gttcccgacg
      781 cttcgctcaa cggcattcag ctgacgcagg cggctctgga gagcgagatc ggtcctgcgg
      841 gcgggggggg gtcgtcgccg tggcgatctt cctgttcgcc ttttcgtcga tcatcggcaa
      901 ttattactac ggcgaagcca acatccgctt catcactccg cggccgtggg cgctgacgct
      961 ctaccggctg ctggtcggcg cgatggtgct cttcggatcc gtggcgacgc tcgatctggc
     1021 atggagcctg gccgatgtga cgatggcgct gatgacgctg tgcaatctgg cggcgatcgt
     1081 gctgctgggc cgtcaggcct tcctgctgct ggccgactac acggcccaga agcggcaggg
     1141 catcaaaaat ccggtcttca cgaaagaccg gattcccgaa ttgaaagaca aggccgagtg
     1201 ctggtagcgc attactccac cagccggcgc gtcacgctcc accgctgcat cagccgcacc
     1261 accgcccagc tcacggcgaa ggccccgcag gcgatcagcg cgatgcgcgc cagcgcggga
     1321 agtcccggaa tccgctgcac gaggtcgtag ccggcctgaa cgaagatgaa gtggcagagg
     1381 tagatgccga acgtcgcgcc cgccagccgc gagagccatg cgcggggccg tgcggcggcc
     1441 ttctgcacga ggatgaagac cggggcggtc atcataaaga cgttgatgcc ggcgaaatac
     1501 cagacgattt cgaggtaggc gtagttgccc gggaaatgtt tctgcatcac cacgtagccc
     1561 agccccgtgg cgaggtatcc ggcgaggaag gtcgggatgc agatgcccaa tgtcctgcgc
     1621 cagttccatg cgggcgggaa tttcacgagg tagaaggcca gtacgaggta accggcgaag
     1681 cccgagacgt agtggaacgt gccgaactcg ttccagtcgc agacgccgta aagccccgtg
     1741 ttgccgtaat tgcccatgta gccgagcgcc ggggccagca tcttcacgta gggcagcagc
     1801 agcgtcacgc cccagatcgc gaggacgctc tgcaactcgc gccgcgaggc ccgttcgagc
     1861 cacgggctga tcagcggcat gatcagataa agtcctatca gcatatagag ataccagagc
     1921 ggcgtggtgt cgtagcagaa gttgaagacg aaggtccaca tcttgtgcag cgtcgcttcg
     1981 ccggtgaaca gcgcgggggc gatcgacggg ctggaggtcc cgacgtagcg catatacagg
     2041 tagtagagca cgggcagcgt cagcgaccag aagaccagcg ccaggaggac gcggccgatg
     2101 cgcttgcggt agaaagcccc cgttccggtc cgcacgggca gcagcagcac gcccgacatc
     2161 atcacgaaca gcggcacgca ggcccggacg aaactgcctg ccaacgctcc ttgcaggaag
     2221 gtggcgcggt cgctgtcgaa cacggcgacg aaggcgtcgc agctgtggga gaagaccacc
     2281 aggaagcagg cgatcacgcg caggaggtcc acccagccga tgcgttcgcg ggccgggacg
     2341 ttcaggtcgg gaagtcggtt cttcattgtc gcagacaatg gatttccggc gaaggtacga
     2401 aaaaaatccg ggaccgccgt gcgttttcgc cgcggcgtcc cggatttttc gttgtccgca
     2461 ctccctccgg cggacgtccc cttcgggtca gttgaccacc accgtcggga agtattcgcg
     2521 cagcagggcc gcggcgcggt cgagctgttc gggggtgttc tcccgcacgt cgcggagttt
     2581 gtactccagc cccatcgcct cgtacttatg cactccgagg gtgtggtagg ggagcagttc
     2641 gacccgctcg accgacttgt agccgccgag gcgttcgccc agcgcgcgga tgtcggcttc
     2701 ggcgtcgctg atgcccggaa ccagcacgta gcgcagccag aagggttttc cgtgggcttc
     2761 gagccaggcg gccgtgcgga gcgtctgcgc gttttcgcgt ccggtgaggg cgcggtggcg
     2821 ttcgggatcg gcctgcttca cgtccagcag caccaggtcg accagcccca gcagctcctc
     2881 gacggccggg ttccacaccc cgccgttcga gtcgatgcag acgtggatgc cggcctcctt
     2941 caacgacttt acgagcggaa cgagttccgc ggcctggaag gtgggttccc cgcccgagaa
     3001 ggtcacgccg ccgcgccgcc cgaagaaggg cttctggtcg acggccatgc gcaggatctc
     3061 cgcagggtcg gtcaatgtgc ccgctccggc ctcgatcgtg tcggggttgg cgcagtagag
     3121 acagcggaaa ttgcagcctt ggaggaaaac gacgagccgg agtcccggcc cgtcgaatgt
     3181 tcccatggat tcgtaagaat gtacacgaag catgattgtt ccttgttttt gaataagggg
     3241 gttcgggcgc aaaccgggtt ccggtatgca aatcctgcgc ccgcccctaa aagcggcttt
     3301 tcaagccgtt acatcctttc gtggaagctg cggctgatga cttcgagctg gtgttcgcgg
     3361 ctcaacttgg tgaagttcac ggcgtagccc gagacgcgga tcgtcagctg cggatatttc
     3421 tcggggtgct ccatggcgtc ttcgagcatc tcgcggttca gcacgttgac gttcaggtgg
     3481 tgtgcgccct tggtgaaata gccgtcgagc atcgtcacga ggttctcgat acgctctccg
     3541 gccgtcgggc cgagcgattt gggcacgatc gagaaggtgt tcgaaatacc gtcctgcgag
     3601 tcgcggtaac gcatcttggc caccgaggcg agcgaagcga tggcgccgct cttgtcgcgg
     3661 ccgtgcatcg ggttggctcc cggggcgaag gccacgccct tgaggcgtcc gtcgggcgtt
     3721 gcgccggtct tcttgccgta catcacgttc gaggtgatcg tcagcagcga cagcgtcggg
     3781 cgggcgttct tgtagacggg cagcttcttc agctcctcgc tgaagtagta gacgaggtcc
     3841 acgcccaggt ggtcgacacg gtcgtcgttg ttgccgtagc aggggaattc accctcgatg
     3901 tcgaaggctt cggtgagacc ctcggcgttg cggcggggcg tcacgcgggc gtatttgatg
     3961 gccgagagcg agtcgagggc gatcgagaga ccggccacgc cgtaggcgag attgatgcgg
     4021 ggatcggtgt cgacgaatgc catctgggcc ttctcgtagt agtacttgtc gtgcatgtag
     4081 tggatgatgt tcatggcctc gttgtagacg cgggcgatct ccttgagcac catcttgtag
     4141 ttgcgcatca cctcctcgaa gcgcagcgga ccctccgaca gggccggaat gcccttgacc
     4201 atcagcgtgc cggtgttctc gcagcgacct tcgttaatgg ccagcagcag ggccttggcc
     4261 aggttggtgc gggcgccgaa gaactggatc tggcggccga tgtcctggaa cgagacgcag
     4321 caggcgatgc cgtagtcgtc cgagtggcgc acttcgcgca tcaggtcgtc gttctcgtac
     4381 tggatcgagc tggtgtcggc cgaaaccttg gcgcagaagt ccttgaatcc ctgcggcagc
     4441 tcggggctcc agagaatcgt gaggttcggt tcgggcgaag gaccgaggtt gtagagcgtt
     4501 tgcaggaagc ggaacgaggt cttcgtcacc ttcgtgcggc cgtcgttgaa gcgtccgccg
     4561 atggcctcgg tcacccacgt cgggtcgccg gcgaagatgt cgttgtagga ctgcatgcgc
     4621 aggtggcgca ccatgcgcag cttgatgacg aactggtcga tcagctcctg ggcgaacgac
     4681 tcgtcgatcg tcccgtgcgc gaggtcgtat tcgatgaaga tgtcgaggaa cgacgatacg
     4741 ttgcccagcg acatggcggc tccgtcctgc tccttgacgg cggccagata ggccatgtag
     4801 acccactgca cggcctcctg cgccgaatgc gccgggcggc tgaggtccag tccgtagtat
     4861 tcgcccatcg tgcggatgtc cttcaaggcc ttgatctgtt cggcgacctc ttcgcgcagg
     4921 cggatgcggg cgtcggtcat cgggccggtg aggttgcgga ggtcctcctg cttggcctcg
     4981 atcaggcggt cggttccgta gagggccaga cggcggtagt cgccgatgat gcggccgcgg
     5041 gcgtagttgt cgggaagacc cgtcaggaag cccagcgagc ggaacgagcg gatctcctcg
     5101 gtgtagacgt cgaacacgcc gtcgttgtgc gtcttgcggt agtgggtgaa gatgtccttc
     5161 accttctcgt cgacctccac gccgttctcc ttgcaggcgc gcgatacgac gttgatgccg
     5221 ccgaa
//
