LOCUS       c00081_gnlPro..         5659 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OFDFEAOM_81.
ACCESSION   c00081_gnlPro..
VERSION     c00081_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 03:08:28
            Original ID  :: gnl|Prokka|OFDFEAOM_81
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5659
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5659
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[940:3932]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      941..3932
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5659
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5659
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..520)
                     /locus_tag="ctg81_1"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRM"
     CDS             complement(941..1693)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.2;
                     E-value: 2.1e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg81_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.2e-30, bitscore:
                     93.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKTLGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQMDFLTEFFTLARAKGVHTA
                     LDTAGQPFRPDDPVYLAAFDRLMANTSLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWIRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEDLLEVSRYPG"
     CDS             complement(1680..3932)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg81_3"
                     /sec_met_domain="Gly_radical (E-value: 1.5e-41, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.8e-271, bitscore:
                     887.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDFKQWEGFEGRIWKEEVNTRDFIQKNYTPYDGDESFLAGPTDA
                     TNKLWGALQKLQKAERAKGGVLDMETEVVSGLTAYGPGYIDPELKDLEKVVGLQTDKPL
                     KRAFMPYGGIKMAEQACTTYGYQPSEKLHEIFTKYTRTHNQAVFDAYTPEMKKARHSHI
                     VTGLPDTYGRGRIVGDYRRVALYGIDYLIKAKQNDFANCGDGTMTEEVVRQREEISMQI
                     SALKGMKEMAAAYGFDISQPAANAKEAVQWLYFGYLAAIKTQNGAAMSVGRISTFLDIY
                     IQRDLDKGILTESQAQELIDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVGGIGMDG
                     RSMVTKNCYRFLHTLENMGPAPEPNLTVLYSSALPEAFKKYAAKVSVNTSSVQYENDDV
                     MKPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKSHEQCGPNYAP
                     ITSEYLTYDEVLPKYVQMLDWLAGLYVNVLNLIQYMHDKYYYEEAEMALIDTDVRRTFA
                     TGIAGFSHVIDSLSAIKYAKVKVVRDENGLATGFETEGDFPKYGNDDDRADEIGVWLLK
                     TFLEMIKKRHTYRNSEATTSILTITSNVVYGKYTGALPDGRAAFTPFAPGATPSYGAEQ
                     NGLLASLNSVAKLPYHWALDGISNTQTINPEALGHSEGERVENLVQVLDGYFDQGAHHL
                     NVNVFGKEKLLDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVLARTCHGVL"
     CDS             complement(4100..5395)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MAAL_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg81_4"
                     /sec_met_domain="MAAL_C (E-value: 3.6e-08, bitscore: 21.2,
                     seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYLEIEKVIGREIIDSRGNPTVEAEVWLADGTIGRGAAPSGAST
                     GEFEALELRDGDKSRFGGKGVSKAVENINTTINEVLAGMDASDIYAVDAAMLKADGTKD
                     KSNLGANAILAVSIAAARAAAISLDVPLYRFLGGVSGNKLPVPMMNILNGGAHATNTVD
                     VQEFMIMPAGAPSFKEGLRWCAEVFHALQALLKSKGLATSVGDEGGFAPDLASDEEAIQ
                     YILEAIEKAGYLPGRDFVLAMDAASSEWKGSKKGEYKLPKCGKVFTSEELVEHWKQLVE
                     KYPIYSIEDGLDEEDWEGWQHMTKELGGKVQLVGDDLFVTNTERLSKGIRLGCGNSILI
                     KLNQIGSVSETLEAIKMAHKAGYTAIASHRSGETEDTTIADLAVALNTCQIKTGAPSRS
                     ERVAKYNQLLRIEEELGGAAQYPGFGAFNIKK"
     CDS             complement(5514..5657)
                     /locus_tag="ctg81_5"
                     /transl_table=11
                     /translation="GEVAEGNEAGRALALSGYEKPKKMFAPSNSPTISAAHCAERVFIS
                     PS"
ORIGIN
        1 ccatgcggct gcgcagctcg cggttgccgc tttccagatc gtccaggttc aggtggctca
       61 gcttgccgta gccggggtcg cttttcaccc ggaagtacac atcgccgttc ttggcaacgt
      121 aggcgtggcc ggagtcgatc agatctttga cgatatcgag aatctggggg atatgctcgg
      181 tagcgcgggg ctgcacagtg ggcttttcgc agttcagccc ggcggcatcc ttcaggtact
      241 cggcctcaaa gcgctgtgcc acctccttca tggaggtgcc ctcttcctgt cccttcttga
      301 tgagcttatc gtcgatatcg gtgatgttgg aaacgtagat caccttgttg ccccggtatt
      361 ccagatagcg gcgcagggtg tcgaacacga tcagcgggcg tgcgttgccg atgtggatat
      421 agttgtacac ggtagggccg cagacgtaga tgcggtactc gccgggcacc tgcggcacaa
      481 attcttcctt ctgtcgggtc agggtgttga agatctgcat ggtcaattct cctttttgtt
      541 ccaaacagaa gccgcaaaac aaaacgcccc cggagcggtg cacaaggcac aactccgaag
      601 gcggttttaa aatcaaaatc gcggttccac ttctgtttgt cgctcaaagc cggtaacggc
      661 ggcgcaccgc acggcgatac ttccccgcag gggttcccgc cgcgtgctgt ccgggcgcac
      721 tttgcgcagc ggtctgcaaa caggcttcca gccagtggcc tgttctctct gcgcagacgg
      781 tgtgcggcgc tactctgccc ggataaacgc atttatcctt ttaccccttt attatagcgg
      841 tgcggtgcag ggaagtcaag gcagaacgaa ccagtgaaaa tgaaattcag ccaagcctac
      901 cggcttggct gaatttcaaa tcaaaaagat gctttcctct ttacccggga taccggctca
      961 cttccagcag atcctctgcg cgctttacct gctctgccgt gggcggtaca gcgtccggca
     1021 ggggataggg gatgcccagc ttttgccact tgaacagccc caaggtgtgg tagggcagca
     1081 cctccacccg ctgcaccgtt ttgagcgagc ggataaagtc tgctgttttg cgcagccctt
     1141 cctcgtcatc ggtcagaccg ggcacgagga cgtgccggat ccacagcggg atgccgaggt
     1201 cggagatgtg ccgcgccatg gcaaggatgt tggcattgtc tttgccggtg agctgccggt
     1261 ggcgctccgg gtcgatctct ttgaggtcta agatcaccag actggtgtta gccatcagac
     1321 ggtcaaatgc ggccagatag accgggtcat cagggcggaa gggctgcccg gcggtgtcca
     1381 gcgccgtgtg cacgcccttg gcgcgcgcca gcgtgaagaa ctcggtcagg aagtccatct
     1441 gccgcagcgg ctcgccgccg ctgaccgtga tgccgccctt tttgccccag tagttgcggt
     1501 agcggtatac ccgctggaac agcgcttctg ccgtccacgc ctcgccgccc tctgcccatg
     1561 tttcggggtt gtggcagtac ttgcaccgca gggcacaccc ctgcaaaaac accacaaagc
     1621 gcacgccggg gccgtccacc gagccgaagg attccagcga gtggacgtac ccaagggtct
     1681 tacaggacgc catggcaggt gcgtgccagc acgtccagct gctgctcgcg ggtcaggtcg
     1741 atgaacttga cagcgtagcc ggaaacgcgg atggtgaagt tggcgtactc ttccttctcg
     1801 gggtgctcca tggcatccag cagcttctcc ttgccaaaga cgttcacgtt caggtggtgt
     1861 gctccctgat cgaagtagcc atccagtacc tgcaccagat tctccacgcg ctcgccctcg
     1921 ctgtgaccca gagcctcggg gttgatggtc tgggtgttgg agatgccgtc cagtgcccag
     1981 tggtagggca gctttgccac ggagttcagc gaggccagca gaccgttctg ctctgcgccg
     2041 tagctggggg tagcgccggg tgcaaacggg gtgaaggcgg cgcggccatc cggcagagcg
     2101 ccggtgtatt tgccgtacac cacgttggag gtgatggtca ggatggaggt ggtagcctcg
     2161 gagttgcggt aggtgtggcg cttcttgatc atctcaagga aggtcttgag cagccacacg
     2221 ccgatctcgt cggcgcggtc gtcatcgttg ccgtacttgg ggaagtcgcc ctcggtctca
     2281 aagccggtgg caaggccgtt ctcgtcgcgc accaccttca ccttggcgta cttgatggcg
     2341 ctcagagagt cgatgacatg ggagaagcct gcaatgccgg tggcaaaggt gcggcgcaca
     2401 tcggtgtcga tgagtgccat ctcggcttcc tcgtagtagt acttgtcgtg catatactgg
     2461 atgaggttca gcacgttgac atacagccct gccagccagt ccagcatctg cacatacttg
     2521 ggcagcacct cgtcataggt caggtactcg ctggtgatgg gggcatagtt ggggccgcac
     2581 tgctcgtgac tcttctcgtc cacgccgccg ttgatggcgt acagcaggca cttggcaagg
     2641 ttggcgcgtg cgccgaagaa ctgcatctcc ttgccggtct gggtagcaga cacgcagcag
     2701 cagatggagt aatcgtcgcc ccagacgggc ttcatcacat catcgttctc gtactggacg
     2761 ctgctggtgt tgacggaaac tttggcggcg tacttcttaa aggcctcggg cagggcagag
     2821 gagtacagca cggtcaggtt gggctccggt gcggggccca tgttttccag cgtgtgcagg
     2881 aagcggtagc agttcttggt gaccatgctg cggccgtcca tgccgatgcc gcccacctcc
     2941 agcgttgccc acacggggtc gccagagaac agctggttgt agctggggat gcgggcaaac
     3001 ttgaccatgc ggaacttcat gaccatgtgg tcgatcagct cctgtgcctg actttcggtc
     3061 aggatgccct tgtccagatc gcgctggata taaatgtcaa ggaaggtgga gatgcggccc
     3121 acgctcatgg cagcgccgtt ctgggtcttg atggcggcca gatagccgaa gtacagccac
     3181 tggacggcct cttttgcgtt ggcggcgggc tgggagatgt cgaagccgta ggcagcagcc
     3241 atctccttca tgcccttcag ggcggagatc tgcatagaga tctcctcgcg ctggcggacg
     3301 acctcctcgg tcatcgtgcc gtcgccgcag ttggcaaagt cgttctgctt ggccttgatg
     3361 aggtagtcga tgccgtacag cgccacgcgg cggtagtcgc cgacgatacg gccgcggcca
     3421 taggtgtcgg gcaggccggt gacgatgtgg ctgtggcggg ccttcttcat ctcgggggtg
     3481 taggcatcga acacggcctg attatgggtg cgggtgtact tggtgaaaat ctcatgcagc
     3541 ttctcgctgg gctggtagcc gtaggtggtg caggcctgct ccgccatctt gatgccgccg
     3601 tagggcatga aggcgcgctt caggggctta tcggtctgca ggccgacgac cttctcgagg
     3661 tccttcagct cggggtcgat gtagccgggg ccgtaggcgg tcaggccgga gacgacctcg
     3721 gtctccatgt ccagcacgcc gcccttggca cgctcggcct tctgcagctt ctgcagcgcg
     3781 ccccacagct tgttggtagc gtcggtagga ccggcgagga aactctcatc gccgtcataa
     3841 ggggtgtagt tcttctggat gaagtcacga gtgttgactt cctctttcca aatgcggcct
     3901 tcaaagcctt cccactgttt gaaatctgtc attgtgcacc tcttttaatg tatggaccgc
     3961 cgggcgtatt tcgcccggct ttgagtatta taccagaatt acagggcttt gtgtgttgtt
     4021 tgggcaacag tttttgagtt gtaggggcgg atgctatcat ccgcccgcgg cattttctct
     4081 cctatctcct ccccagccct tactttttaa tgttaaacgc cccaaatccc ggatactgcg
     4141 cggcgccgcc aagctcctcc tcgatgcgga gaagctggtt gtacttggcg acgcgctcgc
     4201 tgcggctggg ggcgccggtc ttgatctggc aggtgttcag cgcaacggcc aggtcggcaa
     4261 tcgtggtgtc ctcggtctcg ccggagcggt gggacgcaat ggcggtgtaa ccggccttgt
     4321 gcgccatctt gatggcctcc agcgtctcgg aaacagagcc gatctggttc agcttgatga
     4381 ggatcgagtt gccgcagccc aagcgaatgc ccttggacag gcgctcggtg ttcgtgacga
     4441 acaggtcgtc gccgaccagc tggaccttgc cgcccagctc cttcgtcatg tgctgccagc
     4501 cctcccagtc ttcctcgtcg aggccgtcct cgatggagta gatgggatac ttctcgacca
     4561 gctgcttcca gtgctcgacc agctcctcac tggtaaagac cttgccgcac ttgggcagct
     4621 tatactcgcc ctttttgctg cccttccact cgctggaggc agcatccatg gccagcacaa
     4681 aatccctgcc gggcaggtag ccggccttct cgatggcctc aaggatatac tgaatggcct
     4741 cctcgtcgct ggcaaggtcg ggtgcaaagc cgccctcgtc accgacggag gtggccagac
     4801 ccttggattt cagcagcgcc tgcagggcgt ggaacacctc ggcgcaccag cgcagaccct
     4861 ccttgaagct gggcgcaccg gcgggcatga tcatgaattc ctgcacgtcc acggtgttgg
     4921 tcgcatgcgc gccgccgttc aggatgttca tcatgggcac gggcagcttg ttgccggaca
     4981 cgccgcccaa gaagcggtac aggggcacat ccagcgagat ggccgccgcg cgggccgccg
     5041 cgatggaaac cgccagaatg gcgtttgcac cgaggttgga cttatccttg gtgccgtcgg
     5101 ccttcagcat agcggcgtcc acggcgtaga tgtcggaggc atccatgccg gcaagaacct
     5161 cgttgatggt ggtgttgatg ttctcaacag ccttgctgac gcccttgccg ccgaagcggc
     5221 tcttgtcgcc gtcgcgcagc tccagagcct caaattcacc ggtggacgca ccgctggggg
     5281 cagcaccgcg gccaatcgtg ccgtcggcca gccatacctc tgcctcgacg gtggggttgc
     5341 cgcgggagtc gatgatctcg cggccaatga ccttctcaat ttccagatag ctcatggtac
     5401 aaagtccttt ctctataaac tgctttttca gtatgcccgg aatgggataa agcgatactt
     5461 caaggttagc gcaagctaac caatgacaag atagcataga ctaaccgctt ttgtcaagag
     5521 ggagaaataa acacccgttc ggcacagtgg gcagcggaaa ttgttggtga gtttgagggg
     5581 gcgaacattt ttttgggttt ttcgtacccg gaaagtgcca gagcccttcc ggcctcgttt
     5641 ccctcggcca cctcccctg
//
