LOCUS       c00404_gnlPro..         1495 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EBMFKIOM_404.
ACCESSION   c00404_gnlPro..
VERSION     c00404_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 22:30:22
            Original ID  :: gnl|Prokka|EBMFKIOM_404
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1495
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1495
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[97:1494]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      98..1494
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..1495
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..1495
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(98..859)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg404_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-32, bitscore:
                     100.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTEKAPSPVWFIPWNPAAPWTXXXPGIRFVLFLSGCSLRCRYC
                     HNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGAVLKGCR
                     KLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTLEFAERL
                     ASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKWDELKLD
                     YTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(901..1494)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg404_2"
                     /sec_met_domain="Gly_radical (E-value: 6.5e-48, bitscore:
                     149.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 4.4e-22, bitscore:
                     65.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRKLHTYRNSLPTQ
                     SILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSL
                     DGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPEK
                     YPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 gattctgtag tggagggaag agggactctc atccggaaga gggggatgcc tgcgtccggt
       61 ttgctgcggt tggtggcgcc aaagcgcgaa aatccggtta atgcacgcaa tccggaaagc
      121 cgccccgccg gaaaatgccg cggacctgtt ccgccagttc cgcggacgct tccgggacgt
      181 ccttcaaagt atagtccagc ttcaattcat cccatttgaa tctccccagc tggtggaaag
      241 ggagcacgtc cacgcgttcc acgttgccca gaagcgccgc atggcgggag agttcctcaa
      301 tttcttccag attgtccgta acgccaggta ccagcacata cctcagccac acgggcttgc
      361 ggagggaggc caggcgttcc gcaaactcca gtgtagggcg cagttcccct cctgtcaggg
      421 cgcggtaccg tttcggattc catgccttga tatccagcag gaccagatcc gtatccgcca
      481 gcatttcttc acccgcattc acgcccagat ggccggaggt gtccaggcag gtgtgcaggc
      541 ccagtttccg gcagcctttc agcacggcac cggcaaaatc cggctggaag agggggtccc
      601 cgccggagag ggtgacgccg ccgcccgccg cctgcaaaaa atcccggtaa cgggctattt
      661 ccttgaggac atcatctgcg ctcctgagcc ggcccctccg cacgtaggag gtatccggat
      721 tatggcagta gcggcagcgc aggctgcaac cggacaggaa aaggacgaag cggatgccgg
      781 gnnnnnnnnn ngtccacggt gccgcaggat tccacggaat gaaccaaacc ggtgacggag
      841 ctttctccgt ccggatcatg gttttgtgga aaaacctgcg gcatccgctt gcagtccggg
      901 ttaacgggtg tggaaagtgc ggttgatgac ctcctgctgc tgttcccggg tcagcttgat
      961 gaaattcacg gcatagccgg aaacgcggat ggtaagctgc gggtattttt ccgggtgatc
     1021 catggcgtcg agaagcgttt cccgttccag tacgttcaca ttaatgtggt gacctgtagc
     1081 ggcaaagtag gcgtccaaca gggagacgag cttgacgcga cgttcacgtt cctccttgcc
     1141 cagggcctga ggaacgatgg agaaggtgta ggagatgccg tccagggagt cgtcatagga
     1201 cagcttggct acggagagca tggaggccac ggctccgttc ctgtcccttc cgtgcatggg
     1261 gttggctccg ggggcgaacg gttccccggc tcggcggccg tccggcgtgt tgcccgtctt
     1321 tttgccgtac accacgttgg aggtgatcgt caggatggac tgggtgggca gggaattgcg
     1381 gtaagtgtgg agcttgcgca gtttttccat gaaattgctg accaggctgc atgctatgtc
     1441 gtccacgcgc gggtcattgt tcccgtaaca ggggaattcc ccttccgtct tgaag
//
