LOCUS       c00171_gnlPro..         5308 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NDLHDDJE_171.
ACCESSION   c00171_gnlPro..
VERSION     c00171_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:20:08
            Original ID  :: gnl|Prokka|NDLHDDJE_171
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5308
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5308
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1539:5035]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1540..5035
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5308
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5308
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             3..215
                     /locus_tag="ctg171_1"
                     /transl_table=11
                     /translation="LLLRLFRYFKSRKGEPRFFNKRNICLLAAAGAYILTPDEFLFDGI
                     GRLGLLDDAAVILGAVLTVFFPDGD"
     CDS             complement(249..1034)
                     /locus_tag="ctg171_2"
                     /transl_table=11
                     /translation="MVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSADGFRDYFVQLT
                     ECEPARAAKFGMDHYTWLCINPKEAQDVAFARDVMKLIPEFIDRPNVLGIGEIGLNRNT
                     SHEMTIFEEHLDLAVRLNQLVLIHTPHLEDKLKGTVMILSALKNRPDLDPCRVLVDHCE
                     EHTFPLVKEAGYWAGLTLYPTSKLNPKRAADILELYGMDRVWANSAADWGDSDPLALTA
                     LACELRRRGFSRQETERLLLENPETFMGQSPKFRKVSSR"
     CDS             complement(1067..1339)
                     /locus_tag="ctg171_3"
                     /transl_table=11
                     /translation="MKRSKKEVLDADFAAARAGVLDVAAFLDRVDRGEGEADFRYHALM
                     DVLPLLSASGPGRVRAILEALSYRDDTVPETAAAKAACGAPNLSK"
     CDS             1540..2241
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg171_4"
                     /sec_met_domain="SucD_FumR (E-value: 2.5e-14, bitscore:
                     42.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             2263..4227
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg171_5"
                     /sec_met_domain="FAD_binding_2 (E-value: 4.1e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 8.6e-36,
                     bitscore: 111.5, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVXX
                     XXFLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPV
                     LHKEPLTFENVHLAIRSYK"
     CDS             4268..5035
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg171_6"
                     /sec_met_domain="Fer2_3 (E-value: 2.1e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 7.2e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 4.1e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 agctgctact gcgcctgttc cggtatttca aaagcagaaa aggagaaccg cgttttttca
       61 acaaacggaa tatatgcctt ctggccgccg ccggcgccta catcctgact cccgacgaat
      121 tcctgtttga cggaatcggc aggctgggcc ttctggatga cgcggcggtc atcctgggcg
      181 ccgtcctcac cgtctttttc ccggacgggg actagagggc cgtaccctcc gccgcgcctc
      241 gggcggaatt agcgggacga cactttccgg aacttcgggg actgtcccat gaatgtctcc
      301 ggattttcca ggagaagacg ttccgtttcc tgccgggaaa aaccgcgcct gcgcagctcg
      361 caggccaggg ccgtcagcgc caggggatcg gaatcccccc agtccgccgc ggaattggcc
      421 cagacacggt ccattccgta caattccaga atatccgccg cccgcttggg attcagctta
      481 ctggtgggat aaagggtcag cccggcccag taaccggcct ccttcaccag cgggaaggta
      541 tgttcctcac agtgatccac cagcacgcgg caagggtcca gatccggacg gtttttcagg
      601 gcggaaagaa tcattacggt ccccttaagc ttgtcttcca gatggggagt gtgaatcagc
      661 acaagctggt tgaggcgcac cgccagatcc agatgctctt caaaaatagt catttcatgg
      721 gaagtattcc ggttcagccc tatttccccg atgccgagga cattgggccg gtcaatgaac
      781 tccggaatca gtttcatcac atcccgcgcg aaagccacgt cctgcgcctc cttgggattg
      841 atgcacagcc atgtgtaatg gtccatgccg aacttggccg ccctggcggg ttcgcattcc
      901 gtaagctgta cgaaataatc ccggaaaccg tccgcggacg aacggtcgta accggcccag
      961 aacgccggtt cccctaccgc caggcagccg gcctgcgcca tggcccggta ttcgtccgtg
     1021 gtgcggcaca ccatatgtat atgaggttct atgaaagcca tcattcttat ttgctgaggt
     1081 tcggggctcc gcaggccgct ttggctgcgg cggtttccgg cacggtgtca tccctgtagg
     1141 aaagggcttc cagaatggcc cggacgcgtc cggggccgga tgcggacaag aggggaagaa
     1201 catccatcag ggcatggtag cggaaatccg cctccccttc cccgcggtcc acccggtcca
     1261 gaaatgcggc cacatccaga acgccggcgc gcgccgctgc gaaatccgcg tccagcacct
     1321 cctttttact tcttttcatg cacagacgat acgcctatgc ggaaagcctc gtcaagccgg
     1381 aacaggcaac aaaatgataa ccattccaaa ctgataaaca gcaacctctt tccccggttt
     1441 ttcaaaaatc cgaaaagcgc cggaaattca agaagaggct tgccatccgg gcttgaaccg
     1501 ggcatcctgt gaagacgcat ttatttcatt agagcgccta tgaatgcact agtaaaaacc
     1561 atatgtacat tcgtgacatc ctccatcggc cgcaaaatca ttgtggctct gacggggtta
     1621 tgtttggtct tgttccttgc gggacacctg gcaggcaacc ttctcatctt cggaggccct
     1681 gaatggatca acacctacgc ccacggcctg cactccatgc cggaagcagc cctctgggga
     1741 atccgcgcgg ggcttgcggt gatcttcatc atccacgtct ggctgaccat ccagctgaag
     1801 ctggaaaacc atgcggcccg cgaaccctac gtgttcaaga acaccattaa ggcaacgctc
     1861 tcctcccgct acatgatcta taccggtttg accgtcctgg tgttcctgat ctaccacctg
     1921 taccagtaca ccctgcgcgt gggatacgac cccgcccagt tcaccacctt tatttccgac
     1981 ggtacggtgg aaacctttga cgtgtacaag atgatcgtga ccggattctc caacgtatgg
     2041 tgctccgcct tctacatcct ggccgttctg atgctgttca gccatctacg ccacggggta
     2101 cagtccatct tccagactgt gggcgtcgat tcccgcaaaa tccggccctt ctacaacttt
     2161 atcgccatcg cctacggcgc gattatctgc ctgggcttca tctccgtgcc ggtctcggtt
     2221 cttctgggta tcatcaaata accatcaact cctctcaaag tcatgattaa ttttcccgta
     2281 agcgactgga tgccggatgc caaaattccc tccggcccta ttcaggacaa atggtccaag
     2341 tacaaacttg agtccaaact gatcaatccg aacaacaagc gcaagtacac ggtgcttgtg
     2401 gtaggttccg gcctggctgg cgcctccgct gccgccacgc ttgccgaact tggctacaac
     2461 gtcaaatgct tctgctacca ggacagcccc cgccgcgccc actccattgc ggcccagggc
     2521 ggcattaacg cagccaagaa ctaccagaac gacggcgact ccgtttaccg cctgttttac
     2581 gacaccgtta aaggcggcga cttccgcgcc cgtgaagcca atgtctaccg tctggcggaa
     2641 gtctcctcca acatcattga ccagtgtgtg gcccagggcg ttcccttcgg ccgtgaatac
     2701 ggcggcctgc tggacaaccg ctccttcggc ggcgcccagc tcaagcgaac cttctacgca
     2761 cgcggacaga cgggccagca gctcctgctg ggtgcctacc aggccctgga aaaggaagtg
     2821 gccaagggac acattgaaat gttctcccgc catgaaatga tggatctggt gctggtggac
     2881 ggcgaagcca agggcatcgt cacccgcaat ctggttaccg gcgaaatcaa atcctacgca
     2941 ggggataccg tcctgctctg cacgggcggc tacggcaacg tgttcttcct gtccaccaac
     3001 gcgatgggct gcaacgtcac tgccgcctac aaggcctaca agcgcggtgc cttcttcgcc
     3061 aacccctgct tcacgcaaat ccaccccacc tgcattccgg tcaagggcga ctaccagtcc
     3121 aaactgacgc tgatgtcgga atccctccgc aacagtggcc gcatctgggt gcccaagtcc
     3181 cgtgaaaccg cagaaaaaat ccgtaaaaag gaaatcaagc cttccgatgt tcccgaagaa
     3241 gaacgggact actacctgga acgcaagtat ccctccttcg gcaacctggc tccgcgcgac
     3301 atttcctccc gcgccgccaa ggaagcctgc gacgacggcc gcggcgtggc cgtcaccggc
     3361 aagggcgtct tcctggattt tgcggatgaa atcaagcgca tgggccgcga atggattgac
     3421 ggccaatacg gcaatctgtt tgacatgtac gaagaaatca cggacgacga cccccatgaa
     3481 acgcccatga tgatctaccc cgcctcccac tacaccatgg gcggcctgtg ggtggactac
     3541 aacctcatgt ccaacgtgaa ggggctgcac gtcctcggtg aagccaactt ctccgaccac
     3601 ggcgccaacc gcctgggagc ctccgccctg atgcagggcc tgtctgacgg ctacttcgtc
     3661 attcccgcca cgttgcccgc ttacctgacg accaccaagc ccggcgccgt caccaccgcc
     3721 gccccggaat tcaaggctgc ggaagacgaa gtgaaggaac gcatcaacaa gctcctgaat
     3781 gtgaagggca ccaagacggt ggactccttc caccgggaac tgggcctcat catgtgggaa
     3841 gactgcggca tggcccgcac ggaagaaagc ctgacccacg ccattgaacg cattccccag
     3901 ctccgcaagg aattctggga aaacgtgcgc gtaaccggca cggcggacgg catcaacgcg
     3961 gaactggaaa aagcctgccg cgtggnnnnn nnnnncttcc tggaattcgc ccagctgctc
     4021 tgctatgacg ccctgaaccg ccaggaatcc tgcggcgccc acttccgcgt ggaataccag
     4081 ctgccggacg gcgaagccaa gcgcaacgac gatgaatacg cctatgtagc cgcctgggaa
     4141 tacactggcg tggacagcct gcctgttctc cacaaggaac cgctgacctt cgagaacgtg
     4201 caccttgcca tccgcagcta caagtaaaga tagccaatac attaacactc aaaagaaaga
     4261 ttataacatg gctaaaacac tcaatctcac tctcaaggtc tggcgccagg aaaaccggga
     4321 agccaagggc cgcattgaga cctatgccgc caaggacatt ccggtggacg cctccttcct
     4381 ggaaatgctg gacatcgtca atgaaggcct ggtcaaggaa ggcaaggaac ccatccattt
     4441 tgaccacgac tgccgggaag gcatctgcgg catgtgctcc ctgaccatca acggcattcc
     4501 ccacgggccg gaacgccagg tgaccacttg ccagcttcac atgcgcaagt tcaaggacgg
     4561 cgataccgtc tggattgaac ccttccgcgc gaaggctttc ccaatcctgc gggacctgat
     4621 ggtggaccgc tccgccctgg accgcatcat cgccgccgga ggctatattg acgtgcgtac
     4681 cggttccgcg ccgaacgcca acaacatcgc cgtggaaaaa gtgaaggcgg acgccgcctt
     4741 tgacgcggcc gcctgcatcg gctgcggcgc ctgcgtggcc tcctgtaaaa acgcttccgc
     4801 catgctgttc acctccgcca agatcgccca tctcaatctg ctgccgcagg gccagccgga
     4861 acgttccaag cgcgtcctgg ccatgatgaa gcagatggaa gcggaaggct ttggcaactg
     4921 cacgaaccag tatgaatgtg aagccgcctg cccgaaaggc atcagcgtgg acaacatcgc
     4981 ccgcctcaac cgcgacttca tgcgggccaa cgccaaggaa ggcctctgtt tctaaagaag
     5041 attccttctt aagacgctct gtaaaaactc ccggcggcat cggctgtcgg gagttttttc
     5101 attaccagat ttccttgaac caggaataaa ataccagaca gttgcttcct catcgtcgcg
     5161 cagcgtaaaa aattgttaca ccggaaggca cccagtaatc caggaaaccg tacttggaaa
     5221 tgccggacga aacctaagca ggccccgtcc gacagcgacg gccccatctt tcttcgagag
     5281 agaaaaacat tgattacagg aattagac
//
