LOCUS       c00133_gnlPro..         5359 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IHAFMDNN_133.
ACCESSION   c00133_gnlPro..
VERSION     c00133_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:29:18
            Original ID  :: gnl|Prokka|IHAFMDNN_133
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5359
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5359
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3989]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3989
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5359
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5359
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..155)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_1"
                     /sec_met_domain="HMGL-like (E-value: 7.2e-12, bitscore:
                     34.0, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGY
                     YAVECW"
     CDS             complement(201..1349)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_2"
                     /sec_met_domain="OAD_beta (E-value: 7.2e-159, bitscore:
                     517.0, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYFTETMGNLLHQTAFFNLTWGNFVMILVACIFLYLAIAKGFEP
                     LLLVPIAFGMLLVNIYPDIFAAPEQMSNGVGGLFHYFYTLDEWSILPSLIFLGVGAMTD
                     FGPLIANPISFLLGAAAQFGIFGAYFLAILMGFNDKAAAAVSIIGGADGPTSIFLAGKL
                     GQSGLMGPIAVAAYSYMALVPIIQPPIMKLFTTKKERAIKMQNLRPVSKLEKILFPIVV
                     TVVVCLILPTTAPLVGMLMLGNLFRECGVVRQLSETASNALMYIVVILLGTSVGASTSA
                     EAFLNATTLKIVALGLIAFMFGTAAGVLFGKLLCFITKGKINPLIGSAGVSAVPMAARV
                     SQKVGAEEDPTNFLLMHAMGPNVAGVIGTAVAAGVFMAIFGI"
     CDS             complement(1366..1737)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 57.7; E-value:
                     2.6e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2.5e-19, bitscore:
                     57.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSYTITVNGNVYDVTVEENGAVSAPAAAPRRAAAPAPAAAPKAA
                     APAGGAGNVKIEAGAAGKVFKIESSVGASVKKGDTILVLEIMKMETPVVATEDGTVASI
                     NVSVGDMVEAGALLATLNN"
     CDS             complement(1803..2543)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_4"
                     /sec_met_domain="OAD_gamma (E-value: 2.8e-28, bitscore:
                     87.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKLSLMLCLCFMVLAMTACGTDPKSVDYFGMSYSDIQDNMEQT
                     VSALVSFSDEDIQSGAEYYDSNGMDAFAHLLTSWGETVSDLGSYQGLGDLTVTKAQKTV
                     TADQVLHFSDRDVVVSYVYEYNYETEAPELTDASADLVYSLGEKMGKAGMNTLMGMGTV
                     FVVLILISLIISCFKVIPYLQNKKANAGAKKDVADPVVEQIEQREETSQLTDDLELVAV
                     ISAAIAAAEGTSADGFVVRSIHRR"
     CDS             complement(2556..3989)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg133_5"
                     /sec_met_domain="CT_Vparvula (E-value: 1.3e-126, bitscore:
                     411.7, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSSNDSLAMQRITKLVDENSFMEIGSLVTARNTDFNLADTDTPS
                     DGVITGHGLVDGNLVFVYSQNATVLNGTIGEMHAKKIAAVYDMAMKMGAPVIGFIDCAG
                     MRLQESVDALNGFGEIYAKEVAASGVVPQISAIFGSCGGGLAVVPALSDFAFMESKGKM
                     FINSPNAIEGNRIEKCDTSSAEFQSEKNGCIDAVGTEDEIIAEIRELVSVLPLNNEGDV
                     YTSECQDDLNRACENMAAMKGDPRYLLSEISDGHVFFETKKNYAKDMVTGFIQLNGMTV
                     GAVANCSEVYDEEGKKAEEFSGALTARGCNKAAEFVNFCDAFEIPVLSLTNVKGYAATV
                     CAEKGLAKALARMTMAFADATCPKVNLITGNAIGSAYVTMNSKAIGADFTYAWEDAKIG
                     MMDGELAAKIMYADASADELAAKAKEYDALQSSVMTAARRGYVDLILDPADTRKYLVDA
                     FELLYTKCAGVPDKKHGTK"
     CDS             complement(4167..5357)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S11"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg133_6"
                     /sec_met_domain="Peptidase_S11 (E-value: 3.1e-51, bitscore:
                     162.8, seeds: 11, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GIQIVEAFDQKENVRMEKVIEKPDQNSLYAKAAVLMDADSGRILY
                     EKNGHQAMANASTTKILTCIIALENCDLDSEVTVSTLAASQPKVHLGMREGQKFYLKDL
                     LYGLMLESYNDCAVAIAEHIAGTTGDFAKLMNDKAEEIGCEDSYFITPNGLDAKNENGF
                     HHTTAADLSLIMRYCIKTSEMAEQFLTITREGQYQFSDIDGKNSYTCCNHNAFLQMMDG
                     ALSGKTGFTGNAGYCYVGALKRDDRTFIVALLACGWPNNKTYKWADTKKLMQYGISRFR
                     KISLDEIELDAGEYAPVQVVDGQGTKIGEEVTTELEVAPVKENVELLIEKDQEVHIEYP
                     LSRKLYAPVTEGDFIGEITYLLGDEVLAKRVVTVKNTVKRIDFCWCVKKTAELFPM"
ORIGIN
        1 ccccagcact caacagcata gtatccaacc ttgtccatct tatctacgat tggaagcatc
       61 tgctctgttg tcattcttgt tgcgatcaga gactgatgtg catcacgcag tactgtctct
      121 gttattttta aaggtttttt aacaacttca gccatttttt cctccttata cagcgaacct
      181 gtttttgttc tgccgtctga ttaaattcca aagattgcca tgaatacacc ggctgctact
      241 gctgtaccga taacaccggc aacgtttggt cccattgcat gcataagtaa aaagttcgta
      301 ggatcttctt ctgcaccaac tttctgtgat acacgcgcag ccataggaac tgcggataca
      361 ccggcagaac cgatgagtgg gttgatcttg ccttttgtaa taaagcaaag taatttaccg
      421 aacaatactc cggctgctgt accaaacata aatgcaatca gtccaagtgc aacgatcttt
      481 aaagttgttg catttaagaa agcctctgca cttgtggatg caccaacgga tgtaccaagt
      541 aagataacta cgatatacat caatgcatta gatgctgtct cagataactg acgaaccaca
      601 ccacactcac ggaacaggtt accaagcatt aacataccaa cgagcggggc tgttgtcgga
      661 aggatcagac atacaacaac ggttaccacg attgggaata agattttctc aagtttggat
      721 accggacgta agttctgcat cttgatcgca cgttcctttt tcgttgtaaa taacttcatg
      781 attggtggct gaatgatcgg caccaatgcc atgtaagagt atgctgctac agcgatcgga
      841 cccataagac cggactgtcc aagttttcca gcaaggaaaa tggaagtagg accatctgca
      901 ccaccgatga tagaaacggc tgctgctgct ttatcattga atcccatcag gattgcaagg
      961 aaatatgcgc cgaaaatacc aaactgtgct gctgctccta aaaggaagct gatcgggttt
     1021 gcgatcagag gtccaaaatc ggtcatcgca ccaacgccaa ggaaaataag ggatggcagg
     1081 attgaccatt catctaaggt ataaaaataa tggaataaac caccgacacc gttactcatc
     1141 tgctccggag ctgcaaagat atccggataa atgtttacca gaagcatacc aaatgcaatc
     1201 ggaacgagta acaacggttc aaaccctttt gcaatcgcca gatacaggaa aatacatgca
     1261 actaagatca ttacgaaatt tccccatgtc aggttaaaaa aggcagtctg atgaaggagg
     1321 ttgcccattg tctcagtaaa ataactcatt gttatcctcc taaaattagt tgtttaaagt
     1381 tgccagtaat gcacctgctt ctaccatgtc accaacactt acgttgatgc ttgctactgt
     1441 accatcttcg gtagcaacaa ccggtgtctc cattttcatg atttcaagaa ctaagattgt
     1501 gtcacctttc tttacagatg cgccaacaga agactcgatc ttaaatactt ttcctgctgc
     1561 acctgcttcg atctttacgt tacctgcgcc acctgccggt gctgctgcct tcggagctgc
     1621 tgccggagcc ggagctgctg cacgtctcgg tgctgctgcc ggtgcactta cagcaccatt
     1681 ctcttctaca gtaacatcat aaacatttcc atttacggta attgtatagc ttttcattga
     1741 atcatcctcc tgtgatcatt acttttttac gacatatatt tattataact tgtctgcttc
     1801 gcctatcttc tatggataga acgtactacg aatccatctg ctgatgttcc ttctgccgct
     1861 gcgattgctg cagaaataac tgcaaccagt tctaaatcat cagtaagctg ggatgtctcc
     1921 tcacgctgct cgatctgttc tacaaccgga tctgccacat ctttcttcgc accagcattt
     1981 gcttttttgt tctgtaaata cggaatcact ttaaagcagc tgatgatcag actgatcagg
     2041 ataagaacta caaatacggt tcccattccc atcaatgtat tcataccggc ttttcccatt
     2101 ttctcgccaa gtgaatacac aagatctgca cttgcatctg taagttccgg tgcttctgtc
     2161 tcgtaattgt attcatatac ataagaaaca actacatctc tgtctgagaa atgtaaaacc
     2221 tggtctgcag tcacagtctt ctgtgctttt gtcacagtaa gatctccaag tccctgatag
     2281 gaacccagat ctgaaactgt ttcaccccag gatgtcagta agtgggcaaa tgcatccatt
     2341 ccgtttgagt cataatattc tgcaccactc tgtatatctt catctgaaaa cgaaacaagc
     2401 gcagatactg tctgctccat gttatcctgg atatcagaat aactcatacc aaaataatca
     2461 acgctcttag gatccgttcc acatgctgtc attgccagaa ccataaagca aagacaaagc
     2521 ataagagata attttttctt cataacttca cctttctatt ttgttccatg tttcttgtcc
     2581 ggtacacctg cacattttgt gtataataat tcaaatgcat ctacaaggta ttttctggtg
     2641 tctgccggat ctaaaataag atctacataa ccacgtcttg ctgcagtcat aacacttgac
     2701 tgtaatgcat catattcttt tgcttttgct gcaagttcat ctgcagatgc atctgcgtac
     2761 atgatctttg ctgcaagctc gccatccatc ataccgatct ttgcatcttc ccatgcatat
     2821 gtaaagtctg ctccgattgc tttagagttc attgtaacat aagcgcttcc gattgcattt
     2881 ccggtgatca ggtttacttt cgggcaggtt gcatcagcga atgccattgt catgcgtgca
     2941 agtgcttttg caagtccttt ttctgcacag acagtagctg cataaccttt tacgtttgtt
     3001 aaagacaata ccgggatctc gaacgcatca cagaagttaa cgaactctgc tgccttgttg
     3061 catcctcttg ctgtcagagc tccggagaac tcctctgctt ttttgccctc ttcatcgtaa
     3121 acttctgagc agtttgcaac agcgccgact gtcattccgt ttaactggat aaatccggtt
     3181 accatatctt ttgcatagtt tttctttgtt tcaaagaata catgtccgtc tgagatctca
     3241 cttaataaat atcttggatc gcctttcatg gctgccatgt tctcacatgc acggttcaaa
     3301 tcatcctgac actcagatgt gtatacatct ccttcattat ttaaaggaag gacgcttact
     3361 aactcgcgga tctctgcaat gatctcatcc tctgttccaa cagcatcgat acaaccgttt
     3421 ttctcactct ggaactctgc ggaagaagta tcgcattttt caatacggtt accctcgatc
     3481 gcattcgggg aattgatgaa catttttcct ttgctctcca taaatgcgaa atcagataat
     3541 gcaggaacta ctgcaagtcc accgccacag cttccgaaaa tagcggaaat ctgcggaaca
     3601 acgccggatg ctgcaacctc ctttgcgtag atctcaccaa atccgtttaa cgcatccaca
     3661 gattcctgta aacgcattcc ggcgcaatcg ataaatccga tcaccggtgc tcccattttc
     3721 atcgccatgt catatacggc tgcaatcttc tttgcatgca tctctccgat agtaccgttt
     3781 aatacagttg cattctggct gtatacaaat accagattgc catccaccag accatgtccg
     3841 gtaattacac catcggacgg tgtatctgtg tctgcaagat taaagtctgt atttcttgcg
     3901 gttacgagag aaccgatttc catgaaactg ttctcatcga ctaacttggt aatacgctgc
     3961 attgccaagc tatcattact actattcatc ctttttgccc tccatctata aagtttgtta
     4021 ccacatcttt tgacatggta catagtgaat aatgtaaaaa tataacaaat tccgctagga
     4081 taaattatag atggtcagtg acaaattttc aagagaattg ttaatttttt gacagttttt
     4141 ttttgcatac aaggaattta gggcttttac attggaaata attctgctgt tttttttaca
     4201 caccagcaaa aatcgattct ttttaccgta ttcttcactg tgaccacacg ttttgccaga
     4261 acctcatctc ccagaaggta tgttatctca ccgataaaat ctccttccgt taccggtgca
     4321 taaagtttcc gggataatgg atactctatg tgtacttcct ggtctttttc aatcaatagt
     4381 tccacatttt ctttcaccgg cgcaacttca agttctgttg ttacctcttc gccgatcttt
     4441 gtgccctgtc cgtccaccac ctgtaccgga gcatattccc ctgcgtccag ttcaatctca
     4501 tccagactta ttttccggaa ccgggaaatt ccatactgca taagtttttt tgtatctgcc
     4561 cacttatatg ttttattatt cggccacccg caggcaagca gtgcaacgat aaaagttctg
     4621 tcatcacgtt ttaatgcccc gacataacaa tatcccgcat taccggtaaa accggtcttg
     4681 cctgacaatg ccccgtccat catctgcaaa aatgcattat gattacagca ggtgtaactg
     4741 tttttaccat ctatatcaga aaactgatac tgtccttccc ttgtgattgt aagaaattgc
     4801 tctgccattt ctgatgtttt gatgcagtac cgcatgatca aagaaagatc tgccgctgtt
     4861 gtgtgatgaa agccattttc atttttggca tccagtccgt tcggtgtgat aaaataactg
     4921 tcctcacagc cgatctcctc tgctttatcg ttcataagtt ttgcaaaatc acccgtcgtg
     4981 cctgcaatgt gttctgcaat cgccacagcg cagtcattat aagattccaa catcagtccg
     5041 tacagcagat ctttcagata aaacttctgc ccctcccgca tacccagatg aactttcggc
     5101 tgtgacgctg caagcgtact cacggtcact tcgctgtcca ggtcgcagtt ttccagtgca
     5161 atgatacatg tcagtatttt ggtcgtactg gcattcgcca tcgcctggtg cccgtttttt
     5221 tcatacagga tacgcccgga atccgcatcc ataagcacag ctgcttttgc atacagggaa
     5281 ttttgatccg gcttttctat cactttctcc atccgcacat tttctttttg atcaaaagcc
     5341 tccacgatct gtatcccat
//
