LOCUS       c00341_gnlPro..         2475 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IHAFMDNN_341.
ACCESSION   c00341_gnlPro..
VERSION     c00341_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:29:18
            Original ID  :: gnl|Prokka|IHAFMDNN_341
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2475
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2475
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2220]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2220
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..2475
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2475
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             complement(2..1258)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Epimerase"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg341_1"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 1.6e-45, bitscore:
                     143.9, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 5.7e-36,
                     bitscore: 111.4, seeds: 81, tool: rule-based-clusters)"
                     /sec_met_domain="Epimerase (E-value: 2.5e-08, bitscore:
                     21.3, seeds: 96, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRLLIIGCGGVAGVAIHKCCQNSKTFTEICIASRTKSKCDALKE
                     KLEKTTDTVITTAQVDADNTEELIALIKKYQPDAVLNVALPYQDLTIMDACLACKVDYI
                     DTANYEPEDTDDKEWRAIYEKRCKEEGFTAYFDYSWQWAYREKFKEAGITALLGSGFDP
                     GVTSVFTAYALKHYFDEIETIDILDCNGGDHGYPFATNFNPEINLREVSANGSYWENGH
                     WVETKPMEIKREYNFPQVGEKDMYLLHHEEIESLAKNVPGVKRIRFFMTFGQSYLTHMK
                     CLENVGMLSTSPIEYEGREIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGKKDGKEK
                     TIYIYNVCDHQECYKEVGSQAISYTTGVPAMIGAALVVDKVWDKDGVFNIEEFDPDPFM
                     DMLNQYGLPWVVDEDPQIVE"
     CDS             complement(1357..2220)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synth"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synt_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg341_2"
                     /sec_met_domain="Spermine_synth (E-value: 8.2e-53,
                     bitscore: 166.3, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Spermine_synt_N (E-value: 6.6e-14,
                     bitscore: 39.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELWFSDYHTEKVKVSVKVQKQLFGLQTEFQRIDVFDSDEFGRFL
                     SSDGSIVFSEKDEFVYDEMIVHVPMAVHPHVKKVLVIGGGDGGVARELGYYDEIEQIDV
                     VEPDRVFVEVCKEFFPDNACGLSDERVTVYYEDGLKFLRMKHDEYDLIINDAIDPLGHT
                     AGLFTKEFYGNCFRALKDDGIMVYQHGSPFYDEDEESCRGMHRKASHSFPISRVYQAHI
                     PTCSSGYWLFGFASKKYHPLDDLDAKRWKERKIKTWYYTTNLHKGAFMLPRYVEDMLEE
                     EEGRKK"
     CDS             complement(2288..2473)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg341_3"
                     /sec_met_domain="OKR_DC_1_C (E-value: 3.1e-12, bitscore:
                     34.7, seeds: 113, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TAGRICGEFVMCYPPGIPILAPGEMITPEIIEYIVYAKEKGCSMQ
                     GTEDPEVENLNVLAKK"
ORIGIN
        1 attctacgat ctgtggatct tcatccacaa cccacggcag accatactga ttcagcatat
       61 ccataaacgg atccggatca aattcttcga tattaaagac gccgtcttta tcccatactt
      121 tatccacaac cagcgccgca ccgatcattg caggaacacc tgtcgtataa gagattgcct
      181 gggaaccaac ttctttgtag cattcctgat ggtcacacac attatagata taaatcgtct
      241 tttcttttcc gtcttttttt cccgtaaaga tacagccgat atttgttttt ccaaccgtgc
      301 gtggtcctaa agatgccgga tccggaagca gtgcttttaa gaactggatt ggtacgatct
      361 ccctgccctc atactcgatc ggtgatgttg agagcattcc gacattttct aaacatttca
      421 tatgtgtcag atagctctgt ccaaaggtca taaagaaacg gatccgcttt actccaggta
      481 cattttttgc aagggactca atttcctcat gatgcagcag atacatatct ttctcgccaa
      541 cctgtgggaa attatattct cttttaattt ccattggttt tgtttctacc cagtgaccat
      601 tctcccagta agaaccgttt gcggaaacct cacgcaggtt gatctccggg ttaaaattgg
      661 ttgcaaacgg ataaccgtgg tcgccgccgt tacagtctaa gatatcaatc gtctcgatct
      721 catcgaaata atgtttcagg gcataagcgg taaatacact ggtcactccc ggatcaaacc
      781 cgcttccaag caatgccgta attcctgcct ctttgaattt ttcacggtat gcccactgcc
      841 aggaatagtc aaaatatgct gtaaatccct cttctttaca gcgtttttca tagatggcac
      901 gccattcttt gtcatccgta tcctccggct cataatttgc ggtatcgata tagtctactt
      961 tgcatgcaag gcaggcatcc ataattgtta aatcctgata tggcaaagca acatttaaca
     1021 cagcatccgg ctgatatttt ttgatcaggg cgatcagttc ctccgtattg tccgcatcca
     1081 cctgtgctgt tgtgatgaca gtgtccgttg ttttttccag tttctctttt aaggcgtcac
     1141 atttcgattt tgttctgctc gcaatgcaga tctccgtaaa agttttgctg ttctggcagc
     1201 atttgtgaat cgcaactcct gcaactccgc cacatccgat aattaataat cttcccattt
     1261 taatctcctt tcctgcgctg tctttttgcg catctcaagt ttgtgccggg gcacaaatct
     1321 tgcgtgtaaa aaaaataatt tttaatttaa tttttattat ttcttacgtc cttcttcttc
     1381 ctctaacata tcctccacat atcgcggcag cataaatgct cccttgtgca gattggtggt
     1441 ataataccag gtttttatct tccgctcttt ccagcgcttt gcatccagat catcgagcgg
     1501 atgatatttc tttgacgcaa acccgaacag ccagtagccg gaagaacacg tcggtatatg
     1561 tgcctgatac acgcggctga ttggaaagct atgactcgct ttccggtgca taccgcggca
     1621 ggattcctca tcttcatcgt aaaacggact tccatgctga tataccatga ttccgtcatc
     1681 ttttaaggca cggaagcagt ttccgtaaaa ttccttggta aacagtcctg cagtatgtcc
     1741 aagcggatca atcgcatcgt tgatgatcag atcatactcg tcatgtttca tccgcaaaaa
     1801 tttcaatcca tcctcatagt acaccgtcac gcgttcatcc gataacccgc atgcattgtc
     1861 cggaaaaaat tccttgcaga cctcgacaaa tacacggtcc ggctccacga catcgatctg
     1921 ctctatctca tcgtaatagc caagctcccg tgccacgccg ccgtcaccgc cgccgatgac
     1981 taacaccttt ttcacatgcg gatgcaccgc catcggaaca tgcacgatca tctcatcata
     2041 gacaaactca tctttttccg aaaaaacaat actaccgtcg gaacttaaaa aacgcccaaa
     2101 ctcatccgaa tcaaacacat cgatccgctg aaactcggtc tgtaaaccaa ataactgttt
     2161 ctgcaccttg accgacacct tgactttttc cgtgtgataa tccgaaaacc aaagttccat
     2221 gatcttctcc cttcttttga aaaaatcttc ttttcacagg aatctttatt tttgctgatc
     2281 tatgctgtta tttctttgca agcacattca gattttcaac ttccggatcc tccgttccct
     2341 gcatagagca tcccttttct tttgcataaa caatgtactc aatgatctcc ggcgtgatca
     2401 tctcacccgg cgctaaaatc ggaattccag gcggatagca catgacaaat tcaccgcaga
     2461 tccttcccgc tgtct
//
