LOCUS       c00103_gnlPro..         3681 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PDJLIGFF_103.
ACCESSION   c00103_gnlPro..
VERSION     c00103_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:46:02
            Original ID  :: gnl|Prokka|PDJLIGFF_103
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3681
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3681
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[642:3598]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      643..3598
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3681
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3681
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             643..2871
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg103_1"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWSKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLVEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSRPATSAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKIDETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2873..3598
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.8;
                     E-value: 2.8e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg103_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.6e-32, bitscore:
                     98.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANDIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
ORIGIN
        1 ttgcggagag acagggattc gaaccccggg tgcctcgcag cacaacggtt ttcaagaccg
       61 ccgcaatcga ccactctgcc acctctccaa aacttctttt tcagaagtac ttttctttta
      121 aagcggtgca aagatacaaa tcatttttta ttctacaaac ttttctgcac aaaaacagag
      181 caattatacc aaaatttttg tttataccca ctacggaacg aacataacgg catttaggct
      241 atttgttgaa atttatcaag ggcaagacag agttttataa cttactggct atcagtatat
      301 ttacaaagag ccacattcag tatacgattg aaccctattc agtatacgac tgaatggcat
      361 tcactatacg actgaattag gttcagaata cgactgttcc ttaaacctac atcatttgta
      421 aataatgcag attattattc ggttcttgtt tcaattcata aaccacatat aaagaaagta
      481 ttaacatctc tactcccccg gacattcact acctaataat agttatttta tgctgttcaa
      541 cataaaaaga gaagtatctc ttataaaatt acggaattta ttcagtattt ttgcgcgctc
      601 taaaggataa cattatctaa cctactaaca aaaacaaatt ttatggaatt gaacaaaacc
      661 tttaccaacg ggctttggag caaagaaatc aatgtcagtg atttcgtcag taaaaacatc
      721 gcaccctata ccggagatgc atctttcctg cagggaccta ccgaacgtac caaacgtatc
      781 tgggacatct gtctgaaggc tctcgaagaa gaaagaaaca acaatggcgt ccgttcactg
      841 gacaacacca ccgtatctac catcacttct cataaagccg ggtatattga taaagacaat
      901 gaacttattg tcggtttgca gactgacgaa ctgttgaaac gatccatcaa acctttcgga
      961 ggaatcaacg tggtagcaaa agcatgccgt gaaaacggag tggaagtgga cgataaagta
     1021 aaagacatct tcacccatta ccgcaaaaca cataacgacg gcgtattcga tgtatatacc
     1081 gaagaaatcc gttccttccg ttcattggga ttcctgaccg gtcttcccga caactatgct
     1141 cgcggacgta tcatcggtga ctaccgtcgt ctggcactct acggcatcga ccgacttgtc
     1201 gaagccaagc aagaagactt gcgcaatctg acaggtccca tgaccgaagc ccgtatccgt
     1261 ctgcgtgaag aagtagcaga gcaaatcaag gctctgaaag acatcaaagt gatgggcgaa
     1321 tactacggac tcgacctcag ccgccccgca acatcagcgc aagaagctgt gcaatgggta
     1381 tatatggctt atctggctgc cgtgaaagaa caagacggtg ctgccatgtc attgggcaat
     1441 gtatcttcat tcctcgacat cttcatcgag tatgatttgg ctcacggcaa gattgacgaa
     1501 acttttgcac aagaactgat agaccagttc gtcatcaagc tgcgcatggt acgccatctg
     1561 cgtatgcagt cgtacaatga catctttgcc ggagacccga cttgggtgac cgaagccatt
     1621 ggcggccgtt tcaacgacgg acgtgtgaaa gtgaccaaaa cctctttccg ttttctgcaa
     1681 accctataca atctcggtcc ttctcccgaa ccaaacatga cggtactgtg gagcccggaa
     1741 ctaccggaag gtttcaagga tttctgtgcc aaagtttccg tagacacaag ctctatccaa
     1801 tacgagaacg ataacctgat gcgcgaagtg cgcaactgcg acgattacgg cattgcctgc
     1861 tgtgtatcct accaagccat cggcaagcaa atccagttct tcggcgcacg tgccaacctt
     1921 gccaaggccc tgctgcttgc catcaacggt ggccgttgcg aaaacaccgg aactgtcatg
     1981 gtgaaaggta ttcccgtatt gaccggcgag acactgaagt ttgaggaagt tatggccaac
     2041 tacaagaagg tactgactga aatagcccgt gtctacaacg aggccatgaa tatcatccac
     2101 tacatgcatg ataagtatta ttatgagaaa gcccagatgg cattcatcga taccgaccca
     2161 cgcatcaatc tggcatacgg tgttgccggc ctttccatcg ccatagactc actgtccgcc
     2221 atcaaatatg ccaaagtcac tgcacgccgc aacgaaatcg gcctgaccga aggtttcgac
     2281 accgaaggct cattcccctg cttcggcaat aatgacgacc gtgtggacca cctgggagta
     2341 gacctcgttt actatttcag cgaagaactg aaaaagctgc ctgtctacaa gaatgcccgt
     2401 cccacccttt ccttgctgac tatcacctcc aacgtgatgt acggcaagaa gaccggtgcc
     2461 actccggacg gacgcgccaa aggtgttgcc tttgctccgg gtgccaaccc aatgcacgga
     2521 cgtgacaaga acggtgccat cgcttccttg agctccgtag ccaaattgcg ctaccgcgac
     2581 tcgcaggacg gcatcagcaa tacgttctcc attgttccca aatcactggg cccgacaccg
     2641 gaagaacgtg tagagaacct ggtaaccatg atggacggct acttcaccaa aggtgcccac
     2701 catttgaacg taaacgtact gaaccgcgaa atgctggaag atgccatgga acatcctgaa
     2761 aagtatccgc aactcaccat ccgcgtttcc ggctatgccg tgaacttcgt gaagttgagc
     2821 cgcgagcatc agttggaagt catcagccgt tcattccacg aaagaatgta atatgataaa
     2881 tgtacactcc tacgaaagta tgggaacatt cgacgggccg ggtctccggc tcgtcgtttt
     2941 cctgcaagga tgtccgttcc gttgtctgta ttgtgccaat ccggatacga ttgatgtaaa
     3001 aggaggcact cccaccactc cggaagagat tctgcaaatg gctatcagcc aaaaggcatt
     3061 cttcgggaag aaaggaggta ttactttctc cggcggtgag cctaccctgc aagcagaagc
     3121 gctgattcct cttttcaagg agctgaaggc gaacgacatc catatctgtc tggacagtaa
     3181 cggaggcatc tggaatgaaa aggtggaaga gctctttagt ctgactgacc tcgtgctgct
     3241 ggacatcaag caattcaatc ccgaacgcca ccgtacgctg acgggacgaa gcaacgaaca
     3301 gacactccgc accgctgcct ggctcgaaga acatgaacac cccttctggc tgcgctatgt
     3361 actggtcccc ggttacagcg attcggaaga agacacccgc ctgctgggtg aaagtcttgg
     3421 caaataccag cagatacaac gggtggaaat acttccttat caccgtctgg gtgttcacaa
     3481 atacgaagcc atgggatggg attacaaatt gaaagaagtg aaggaaaata ctccggaaca
     3541 actggaacgg gcagaaaaac tgttcaaaga gtatttcacg aatgtgataa ccaactaaaa
     3601 cagaaaagca aggctgtccg gatatgattt gtaatagtca agacttgttc tgacaatacg
     3661 tttactatcg tacaaaaaaa g
//
