LOCUS       c00534_gnlPro..         2915 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BMBNEDIB_534.
ACCESSION   c00534_gnlPro..
VERSION     c00534_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 04:07:03
            Original ID  :: gnl|Prokka|BMBNEDIB_534
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2915
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2915
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[332:2914]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      333..2914
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..2915
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2915
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             186..296
                     /locus_tag="ctg534_1"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKDYNAKLCFTTYNKNKKAPFF"
     CDS             complement(333..1088)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg534_2"
                     /sec_met_domain="Fer2_3 (E-value: 4.5e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 8.9e-13, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.5e-07, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(1119..2000)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg534_3"
                     /sec_met_domain="FAD_binding_2 (E-value: 2e-14, bitscore:
                     41.5, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 6.8e-40,
                     bitscore: 124.2, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VNSTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDNPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGKETVDTIHRKLGHI
                     MWEHIGMARDAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELEKALRVADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     EFVVPQTRNYKK"
     CDS             complement(1997..2914)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 144.4; E-value:
                     1.8e-44)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg534_4"
                     /sec_met_domain="FAD_binding_2 (E-value: 1.1e-45, bitscore:
                     144.4, seeds: 62, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LVNPANKRRLDVIVVGTGLAGASAAASLAEMGFNVLNFCIQDSPR
                     RAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKGGDYRAREANVYRLAEVSNSIIDQCV
                     AQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQTGQQLLLGAYSALSRQIGLGKVKMYT
                     RHEMLDVVKVDGRARGIIARNLITGKIERYAAHAVVVATGGYVNTFFLSTNAMASNGSA
                     AWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKLTLMSESLRNDGRIWVPKKLEDAKAL
                     QAGTKKGKDXXXXPLKSVATQVSA"
ORIGIN
        1 ataaaatact tattagcata tactatatga tctttaaagg caaagatcga cgataaaatg
       61 acgctaaaca atctctattt gcagtatttt atggtccatg tttaaaaggt aggtctataa
      121 acaattaaag cctgtctcac gacaagccct aaccaaactt tgttaacctt aaatctaata
      181 ctattatgaa aaaaccacga tgcaaatata cggacttttc aattcctgtc aaggattata
      241 atgctaaatt atgttttacg acatataaca aaaacaaaaa ggcgcctttc ttttagagaa
      301 agacgccttt tccatgatat actaatcaaa tattagtctt gtaatttagc gcaaatgaac
      361 tcgcggttca aacgagcgat gttgctaacg gagatattct tcggacattc catctcgcaa
      421 gcacgtgtgt tcgtacagtt accgaagcca agctcatcca ttttagcgac catagctttg
      481 gcacggcgag cagcctcgac tttaccttga ggcaacaaag ccagctggct aaccttagcg
      541 gatacgaata acatagcgga accattctta caagcggcag cacaagcgcc acaaccgata
      601 caagcggcag cgtccatggc taagtctgcg tccttctttg ggataggtat cgcattagcg
      661 tcgggtacac cacctgtgtt gacagatgtg aaaccacccg cttggatgat tttgtcgtaa
      721 gcgtagcggt ctaccatcaa gtcgcggata acagggaaac ctgcggaacg ccacggttcg
      781 atcgtgatcg tctctccatc gtggaaacga cgcatatata actggcaagt cgtagctcca
      841 gttgccggtc cgtgcgggtg tccgtttaca tacaatgaac acatgccaca gataccctcg
      901 cggcagtcgt gatcgaatac gatcggttcc ttaccctcgt tgaccaactg ctcgttcaag
      961 atatccaaca tttccaagaa agagacactc tgattgatct tctcaatttt gtatgtctcg
     1021 aactgtcctt tctccttcgg acctctctga cgccaaacct taagtgttac acttatatct
     1081 ttatccatga ttctaatttt ttctgagatt gatgattatt acttcttgta gttacgtgtt
     1141 tgaggaacaa cgaactcata gttcaaatcc tctttcaaca tgacaggatc tttgtcctcg
     1201 ccttggtact cccagcaaga tacgtacatg aacttatcgt catgacgcaa ggcctcacct
     1261 tcttctgtct ggtgctccgt acggaagtga ccaccacaag actcagcgcg gtccaaagca
     1321 tcgtgtgcca tcaatgtacc gatctcgata aagtcagcta cacgtaatgc tttctctaac
     1381 tcggtattca agttgtctgc gttgcctggg atgaatacgt tgctccagaa ctctttcttc
     1441 aagtctttca gcatctggat agcttcttgc aaaccttttg cgtcgcgtgc cataccgata
     1501 tgttcccaca tgatatgacc tagtttgcgg tggatagtgt ctacggtttc tttacccttg
     1561 acgttcatca atttttggat acgatccttg atggcctttt ctgcctcgac gaactccggc
     1621 agatccgtgc tgaagcgagg aacttggatt tggtcggaca aatagttctg gatcgtgtat
     1681 ggtaatacga aataaccatc ggccaatccc tgcatcaaag cggaagcacc taaacggttc
     1741 gctccgtgat cggagaagtt tgcctcaccg atagcgaaca gacccggaat ggaggtcatc
     1801 aactcatagt caacccacaa tccacccatt gagtagtgca atgccgggta gatcatcatc
     1861 ggagtctcgt atggattatc gttcgtgatc tcttcgtaca tatcgaacaa gttaccgtat
     1921 ttctgcttaa cgacctcttt acccaagcga ttgatcgcgt cggagaaatc caagaatacg
     1981 gccaagcccg tgctgttcac gccgaaacct gcgtcgcaac gctctttagc ggcannnnnn
     2041 nnnnatcttt acctttcttc gttccggcct gtagagcttt agcgtcttcc agtttcttcg
     2101 gaacccagat acggccgtcg ttacgcaagg attcggacat caaagtcaat ttagattgga
     2161 agtcaccttt caccggtacg caagtcgggt ggatctgaac catacaagga ttagcgaagt
     2221 aagcgccttt cttgtagcat tgccatgcgg cggaaccgtt agatgccata gcgttggttg
     2281 acaagaagaa cgtattaacg taaccacccg tagctacaac tacagcgtga gcggcgtaac
     2341 gctcgatctt acctgtaatc aagttgcggg cgatgatacc acgagcacga ccgtctacct
     2401 taactacatc caacatctca tgacgagtgt acatcttaac cttacccaaa ccgatctgac
     2461 ggctcaacgc tgaataagcg cctaacaaaa gctgctgacc cgtctggcca cgggcgtaga
     2521 acgtacgcga tacctgagcg ccaccgaatg aacggttatc caacagacca ccatattcgc
     2581 gagcgaaagg aacgccttga gccacgcact ggtcgatgat tgagttagaa acctcagcca
     2641 aacgataaac gttagcctca cgagcacggt agtcaccacc tttgatcgta tcgtagaaca
     2701 aacggtaaac agagtcacca tcattctggt agttcttagc ggcgttgata ccaccttgag
     2761 cggcgataga gtgcgcacgg cggggagaat cttggataca gaagttcaat acgttgaatc
     2821 ccatctctgc caaagatgcg gcagcggaag ctccggccaa ccctgtaccg actacgataa
     2881 catccaaacg acgcttgtta gcggggttca ccaat
//
