LOCUS       c00165_gnlPro..         4969 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BNKKIHOE_165.
ACCESSION   c00165_gnlPro..
VERSION     c00165_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 02:05:24
            Original ID  :: gnl|Prokka|BNKKIHOE_165
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4969
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4969
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[839:3903]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      840..3903
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4969
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4969
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             840..3107
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg165_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6.9e-283, bitscore:
                     927.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPKEWQGFKPGAWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDRLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRKLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             3124..3903
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg165_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-31, bitscore:
                     97.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSAGDVLREIARYRDFLQAAGGGVTLSGGDPLFQPDFTGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPERYRSLTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEGLSRHAALLGNVERVDVLPFHQMGRFKW
                     DELKLDYTLKGTPEASAKLAEQVRGIFRREGFPDCVH"
     CDS             complement(4004..4969)
                     /locus_tag="ctg165_3"
                     /transl_table=11
                     /translation="MELKPYRILVAVPGALDEALATVPLIRALKTVRCDMQVNVICPSA
                     QMGIWKTVPEVTHVLPHDSLKQLREALVADEFYNDGPLDMGVMLDGDMETLKALEPYGP
                     MMFSGLDTHPGVRKYKFRVKAPVLRAAPPAHRVQLYLQLGGLHGLDVGKPSLFPVKKAA
                     PAEGAPILIAPFSRLGSASEWSREQWTELVSLLPGRTVLVALEEDRERAAALAEHLNVE
                     LAVGAPEALFSVMDGASVAVAVDGDFPSLCSFRGLPVVTLFSTRLPDVYRPMGTFNRSL
                     YSHQCCSPCFRKDCDRDVPCNRHIAVREVLDALREISGKE"
ORIGIN
        1 tcatgccggg cattccaatc atacggatcc cgctgatggg gctccggctt cagggttgcg
       61 ggcaacgcgg agggagggat ggaaggtgcg gaaaacgcca cactggcccc cccagcaata
      121 tggcgagcat gtaggagaag aaattcatga taaaaaagtg aaaccggttt ccagtttaag
      181 caaatcctgc cgtgcatcaa gatcgcaggc gccacttttg ccggcagagt aaaaaagacc
      241 actgcccgtc cggcaaatcc cgccgcaaca gcggccaggc gaacaccgca tccggccttt
      301 cctgttccga gagccctgtt gccccaggaa agctcgactc cccaacggag ggtaatgctt
      361 tcaccaaaaa cgacataccc ccgtttgacg gtccgggtcc gttccatcac tgcctcatgg
      421 cgtttttcca gtcatgcacg gcccgctggt tctcctgggg cttcagcgtg ggcggcaatt
      481 catcagcaca ggcaccggag aaacaagcaa cgcaaaaaac tcccgccagc aataaacaaa
      541 ggcatgcata agatttcata acggtaagcg ttgagatact gcaatagaga ttaggaaagt
      601 aaagagaaat aatcttccgt cacttttccg caatcgcatg ttcccctcat tttccgctcc
      661 gcaacggaca gacaggtgaa acatgccaaa gccagcgcac ccgcgttctc ttcgcaaaga
      721 attagccttg ttgtctcccg gaattcacga tataattacg tggcgcggag ttttgccgtt
      781 tccggccttt caacggctgc ggaacgtttt tctccggtct tcttcactac atcctattta
      841 tgatcagcat agtaaaagac ttgaaggagt ccaccgccct tccgaaggaa tggcagggat
      901 tcaagccggg agcatggacg gagtccattg acgtacggga tttcatccag cataactaca
      961 cgccctattc cggcaatgaa gaatttcttt ccggcccctc ccagcgcacg ctccgtttat
     1021 gggatgaact gaaagtcctg ctgaaaaggg aaatagacaa cggcggcgtt ctggatgctg
     1081 atgaagaagt ggtatcctcc atcacctccc acaagcccgg ctacattgac aaggaactgg
     1141 aagtggtcgt aggcctccag acggacgccc ccctgaaacg ggccctgatg ccctttggcg
     1201 gcctgcgcat ggcccagcag gccctggaat cctacgggtt caagatgtgc gagaaaaccg
     1261 cggatatttt caaaaagata cgcaagacgc acaatgaagg cgttttcgac gcctacactt
     1321 ccgatatccg ggcggcccgt tccgccggca ttattaccgg ccttccggac gcctatggcc
     1381 gcggccgcat catcggggac taccgccggg tggccctgta cggcacggac agactgattg
     1441 ccgaacgccg caaggacctc aaaaaccgtg aacattcccc ccttacggat gagctgatcc
     1501 gcctgcgcga agaaatgagc gaacaaatac gcgccctgga agaactcgcc cagctgggcg
     1561 cctcctacgg ctgcgacctc actcgccccg ccgcaaacgc ccgcgaagcc gtgcaatgga
     1621 cgtacctggg ctacctggcc gccgtgaagg aacagaacgg agcggccatg tccctgggcc
     1681 gcgtttccac tttcttcgac atttatttca cccgcgacct ggaacagggg ctcatcacgg
     1741 aagaggaagt tcaggaaatc atcgaccagt tcgtgatgaa gctgcgcatc gtccgtttca
     1801 tccgcacgcc tgattacaat aacctgttct ccggagaccc cacctgggtg acggaatcca
     1861 tcggcggcat gggagaggac gggcgcaccc tcgtcacccg cagttccttc cgcatgctgc
     1921 agaccctgta caacctgggg ccggctccgg aaccgaacct gaccgttctg tggtcccgga
     1981 acctgccgga agccttcaaa agcttctgcg ccaaggtttc cattgaaacg tcctccgtcc
     2041 aatacgagaa cgacgacctg atgcgcccgc actggggcga cgactacggc atcgcatgct
     2101 gcgtgtccgc catgcgcatc ggcaagcaga tgcagttctt cggagcgcgc gccaacctcg
     2161 ccaagtgcct tctctacgcc ctgaacggcg gcgtggatga actcaagggc aaacaggtgg
     2221 ccccgccctc cccccgctac acggaagaaa ttctcaacta tgatgaagtg atggccctgt
     2281 acgacaagat gcaggactgg ctcgccaaaa cttacattga cgcgctgaac atcatccact
     2341 acatgcacga caaatactgc tatgaacgca ttgaaatggc gctgcatgat ccggaaatcc
     2401 tgcgcacgat ggccacggga atcgccgggc tttccgtggc ggcggactcc ctgtccgcca
     2461 tcaagtacgc caccgtaaaa gccatccgca atgaagaggg gctgattgtg gacttcaaga
     2521 cggaagggga attcccctgt tacgggaaca atgacccgcg cgtggacgac atagcatgca
     2581 gcctggtcag caatttcatg gaaaaactgc gcaagctcca cacttaccgc aattccctgc
     2641 ccacccagtc catcctgacg atcacctcca acgtggtgta cggcaaaaag acgggcaaca
     2701 cgccggacgg ccgccgagcc ggggaaccgt tcgcccccgg cgccaacccc atgcacggaa
     2761 gggacaggaa cggagccgtg gcgtccatgc tctccgtagc caagctgtcc tatgacgact
     2821 ccctggacgg catctcctac accttctcca tcgttccgca ggccctgggc aaggaggaaa
     2881 gggaacgccg cgtcaagctc gtctccctgc tggacgccta ctttgccgct acaggtcacc
     2941 acattaatgt gaacgtactg gaacgggaaa cgcttctcga cgccatggat cacccggaaa
     3001 aatacccgca gcttaccatc cgcgtttccg gctatgccgt gaatttcatc aagctgaccc
     3061 gggaacagca gcaggaggtc atcaaccgca ccttccacac ccgctaaccc ggacgacaca
     3121 cggatgccgc aggtttttcc acaaaaccat gatccggacg gagaaagctc cgtcaccggt
     3181 ttggttcatt ccgtggaatc ctgcggcacc gtggacggtc ccggcatccg ctttgtcctt
     3241 ttcctgtccg gctgcagcct gcgctgccgc tactgccaca atccggatac ctcctacgtt
     3301 cggcggggcc ggctcagaag cgcgggtgat gtcctcaggg aaatagcccg ttaccgggat
     3361 tttttgcagg cggcgggcgg cggcgtcacc ctctccggcg gggaccccct cttccaacct
     3421 gattttaccg gcgccgtgct gaaaggctgc cggaaactgg gactgcatac ctgcctggac
     3481 acctccggcc atctgggcgt gaatgcgggc gaagaaatgc tggcggatac ggacctggtc
     3541 ctgctggata tcaaggcatg gaatccggaa cggtaccgct ccctgacagg cggggaactg
     3601 cgccctacgc tggagtttgc ggaacgcctg gcctccctcc gcaagcccgt gtggctgagg
     3661 tatgtgctgg tacccggcgt tacggataat ctggaagaaa ttgagggact ctcccgccat
     3721 gcggcgctcc tgggcaacgt ggaacgcgtg gacgtgctcc ctttccacca gatggggaga
     3781 ttcaaatggg atgaattgaa gctggattat accttgaagg gcaccccgga agcgtccgca
     3841 aaactggcgg aacaggtccg cggcattttc cggcgggaag gctttccgga ttgcgtgcat
     3901 taaccggatt ttcgcgcctt ggcgccatca accgcagcaa accgaataca gatatctccc
     3961 ctcctcatct tccggatgaa aggcccttct cccctttccc aaattattcc tttccggaaa
     4021 tttcccgcaa agcatccagc acttcccgca cggcaatgtg gcggttgcag gggacatccc
     4081 ggtcacaatc cttccggaag cagggggaac agcactggtg gctgtaaagg gaacggttga
     4141 atgtacccat agggcggtac acatccggaa gccgggtgga aaacagagtc accaccggaa
     4201 gcccgcggaa ggaacagagg gacgggaaat cgccatccac ggctaccgcc accgaagcgc
     4261 cgtccatgac ggaaaacaga gcctccggcg cacccacagc cagttccaca ttcaaatgtt
     4321 ccgccagcgc ggcggcgcgc tcccggtctt cttccagagc caccagaacc gtccgtcccg
     4381 gcaaaaggga aaccagttcc gtccactgct cccggctcca ttcactggcg cttcccagac
     4441 gggaaaacgg agcaattaaa atgggggcgc cttccgccgg ggccgctttt ttcacaggga
     4501 acaaagacgg tttaccgacg tccagcccat gcagcccccc caattgcaga taaagctgca
     4561 cccggtgcgc cgggggggcg gcgcgcagga cgggggcctt tacccggaat ttgtatttcc
     4621 ggacgccggg atgcgtgtcc agcccggaga acatcatggg gccatacggt tccagcgctt
     4681 tcagggtttc catatctcca tccagcatga cgcccatatc cagcgggccg tcattgtaaa
     4741 attcatctgc caccaaagct tcccgaagct gcttcaggga atcgtgcggc agcacatggg
     4801 taacctccgg caccgtcttc catattccca tctgggcaga cgggcagata acgttcacct
     4861 gcatgtcgca ccggacggtt ttcagggcac gaatcagggg aacggtcgcc agggcttcat
     4921 ccaacgcccc gggaacggct acaagaatgc ggtacggctt cagctccat
//
