LOCUS       c00117_gnlPro..         5499 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PFOBJKDN_117.
ACCESSION   c00117_gnlPro..
VERSION     c00117_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 23:01:28
            Original ID  :: gnl|Prokka|PFOBJKDN_117
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5499
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5499
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:4316]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..4316
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..5499
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5499
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             3..254
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg117_1"
                     /sec_met_domain="Thiolase_C (E-value: 1.7e-41, bitscore:
                     129.7, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DMDLIEANEAFAAQSIAVARDLKFDMSKVNVNGGAIALGHPVGAS
                     GCRILVTLLHEMQKRDAKRGLATLCIGGGMGCSTIVER"
     CDS             348..1220
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg117_2"
                     /sec_met_domain="HCDH (E-value: 1.2e-35, bitscore: 111.1,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 8.5e-64, bitscore: 203.6,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVGVIGAGTMGQGIAKAFAQVEGYEVALCDIKQEWAEGGKEKIK
                     KGYARLVEKGKMTQEAVDGILAKITPGLKEDLCKDCDLIVEAAFENMEVKKTTFKELDE
                     IAKPDCIFASNTSSLSITEIGNGLNRPMVGMHFFNPADRMKLIEVIAGVNTPDETVSAI
                     KKISEEIGKTPVQVNEAAGFVVNRILIPMINEAAFIKMEGVSDIAGIDAAMKLGANHPM
                     GPLELGDFIGLDICLAIMDVLYKETGDSKYRACPLIRKMVRGGNLGAKSGKGFYVYNAD
                     RTKTPVDAQ"
     CDS             1250..2404
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg117_3"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 3.9e-53, bitscore:
                     168.6, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.4e-31, bitscore:
                     97.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2.7e-40, bitscore:
                     126.6, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFTLDKKHEMARSLFKEFAETEVKPLAQETDETEAFPAETVKKM
                     QKYGFMGIPVPKEYGGQGCDPLTYVMCVEELSKVCATTGVVVSAHTSLCIDPIMTYGTE
                     EQKQKYVKPLATGEKLGAFALTEPGAGTDAQGAQTKAVLDGDEWVLNGSKCFITNGKVA
                     DVYIVIAITSITEDKRGRKKKNFSAFIVDKGAPGFSFGTKEKKMGIRGSSTYELIFEDC
                     RIPKENLLGPEGKGFPIAMHTLDGGRIGIAAQALGIAEGALDRAIAYTKERKQFGRSIA
                     QQQNTQFKLADMAARIEAAQLLVYKAAMAKATQKVYSVEAAKAKLFAAETAMAVTTEVV
                     QLFGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGALLK"
     CDS             2461..3243
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 159.1;
                     E-value: 3.7e-49)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg117_4"
                     /sec_met_domain="ETF (E-value: 4.1e-50, bitscore: 159.1,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVVCIKQVPDTKGGVKFNPDGTLDRAAMLAIMNPDDKAGLEAA
                     LRIKDETGAKVTVLTMGLPKADAVLREALAMGADEAVLVTDRVLGGADTWATSTTIAGA
                     LRNLDYDLIITGRQAIDGDTAQVGPQIAEHLGLPVISYAADIKVEGDSVVVKRQYEDRY
                     HELKAKMPCLVTALSELNEPRYMTPGGIFDAFDKEVTVWGRADLKDVDDSDLGLKGSPT
                     KIAKASDKVAKGAGEKVNLDPAESVAYLIGKFKEKHII"
     CDS             3270..4316
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 161.3;
                     E-value: 7.7e-50)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg117_5"
                     /sec_met_domain="ETF (E-value: 8.6e-51, bitscore: 161.3,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 3.7e-39, bitscore:
                     121.7, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALEEYKGVFVFAQQVDNVLDGVAFELLGKGKDLAKDLGTDVTAV
                     LIGSGVKGLADQLAEYGADKVIVVDDPELKDYRTEPYAHALASVINEFKPEIMLVGATA
                     IGRDLGPTVSARVKTGLTADCTSLEIGDFPLVAAPGKESEQKHNQLLMTRPAFGGNTIA
                     TIACPDNRPQMATVRPGVMQKIAPISGAKANVVEYNPGFTPNNRYVEILNIVKAVKSTA
                     NIMEAKILVSGGRGVGSKENFKLLEDLAEVLGGTVSCSRAVVENGWLPVDLQVGQTGKT
                     VRPQIYFAIGISGAIQHVAGMEDSDLIIAINKDEDAPIFDVADYGLVGDLNKIVPALTT
                     ALKAELGK"
     CDS             complement(4489..4848)
                     /locus_tag="ctg117_6"
                     /transl_table=11
                     /translation="MILDYITPNGNYDGLFSSGGLRIYHVDATLANDEYGAPYLTADNY
                     SPVYDSTNNGRRILRLVNNGNGFYRDGDVIGGTATGFGWYDNNGQLTIDTGYMITVDSV
                     SEDQKNCTITILTNN"
     CDS             complement(4874..5497)
                     /locus_tag="ctg117_7"
                     /transl_table=11
                     /translation="VFTMIQKKNVLNTVPVICLGALLLGAAYGQLHTPMTPPPITALAS
                     DILPTTGNVRALVVVVDFADAKYDTDRRLSDEEISSYLFGTDNTEFYPCESLTSYFDRA
                     SYGSLHMTGNVFHYTAKGTIASYEKTNDGYETLVREVLSGLNDTIDYTNYDSDGDGYID
                     ALCLSVPSGGNADFWYGCTASWWASTLPDLDGEKLRKLSDHTAH"
ORIGIN
        1 atgatatgga cttaatcgaa gcaaacgaag cattcgcagc tcagtctatc gcagtagcaa
       61 gagatctgaa attcgatatg tcaaaagtaa acgtaaacgg tggtgcaatc gcattaggac
      121 acccggtagg tgcttccgga tgccgtatcc ttgttacatt actgcatgag atgcagaaga
      181 gagacgctaa gagaggtctt gctacacttt gcatcggcgg tggtatggga tgctctacaa
      241 tcgttgagag ataatagttt tttatagtaa ttcccgggga atgccttccc ggggattgca
      301 tttttcaaac atacagttaa cataattatt ttcaggaggt aaccgaaatg aaggttggcg
      361 ttattggtgc aggaactatg ggacagggca ttgcaaaagc atttgctcag gtagaaggat
      421 atgaagtagc actttgcgat atcaagcagg agtgggctga aggcggaaaa gagaaaatca
      481 aaaaaggtta tgcaagatta gtagaaaaag gaaaaatgac acaggaagct gttgatggca
      541 tccttgcaaa aatcactcca ggtttaaaag aagacctttg caaagactgt gatctgatcg
      601 tagaggcagc tttcgagaac atggaagtaa agaaaactac tttcaaagaa ttagacgaga
      661 tcgcaaaacc agattgtatc ttcgcttcta acacatccag tctttccatc acagagatcg
      721 gaaacggatt aaaccgtcca atggttggta tgcatttctt caacccggca gaccgtatga
      781 aactgatcga ggttatcgct ggtgtgaata ctccggatga aactgtttcc gctattaaga
      841 aaatctctga agagatcgga aaaactccgg tacaggttaa tgaggctgct ggtttcgttg
      901 taaaccgtat cttaatccca atgatcaacg aagctgcatt catcaaaatg gaaggtgttt
      961 ccgatatcgc tggtatcgat gcagctatga aacttggtgc taaccaccca atgggaccgt
     1021 tagagttagg tgatttcatc ggtcttgata tctgccttgc tatcatggat gtactttaca
     1081 aagagacagg cgacagcaaa tatcgtgcat gcccactgat ccgtaagatg gttcgtggtg
     1141 gtaaccttgg agcaaagagc ggcaagggat tctatgtata caatgctgat cgtacaaaga
     1201 ctccggtcga tgctcagtaa aataaatatt tatggaggaa aattagatca tggatttcac
     1261 tttagacaag aaacatgaaa tggcgcgttc cttattcaag gaatttgctg aaacagaagt
     1321 aaaaccgctt gcacaggaaa cagatgagac agaagctttc ccggctgaaa cggttaagaa
     1381 aatgcagaaa tacggattca tgggaatccc ggttccgaaa gaatatggtg gacagggttg
     1441 tgatcctctt acatacgtta tgtgcgtaga agagttatct aaagtttgcg caaccacagg
     1501 tgttgttgta tctgcacata catctctctg catcgatccg atcatgacat atggtacaga
     1561 agagcagaaa cagaaatatg taaaaccact tgcaacaggc gagaagttag gtgcattcgc
     1621 attaactgag ccgggtgctg gtactgatgc acagggtgca cagacaaaag ctgttttaga
     1681 tggtgacgag tgggtattaa acggatctaa atgtttcatt accaacggta aagttgcaga
     1741 cgtttatatc gtaatcgcaa tcacaagcat cacagaggat aagagaggca gaaagaagaa
     1801 aaacttctct gcattcatcg ttgataaggg agctccaggc ttctcattcg gaacaaaaga
     1861 gaagaagatg ggtatccgtg gatcatctac ttatgagctg atcttcgaag actgcagaat
     1921 cccgaaagag aatctgttag gaccggaagg aaaaggtttc ccgatcgcaa tgcacacact
     1981 tgatggtgga cgtatcggta ttgctgctca ggcacttggt atcgctgagg gcgcattaga
     2041 cagagctatc gcttatacaa aagagagaaa acagtttgga cgttcaatcg cacagcagca
     2101 gaatacacag ttcaaacttg ctgatatggc tgcaagaatc gaagctgcac agcttttagt
     2161 ttacaaagct gcaatggcaa aagctactca gaaagtatat tctgtagaag ctgcaaaagc
     2221 gaaattattc gcagcagaga ctgcaatggc tgttacaact gaggttgttc agttattcgg
     2281 tggatacgga tatatcagag agtacgatgt agagcgtatg atgcgtgatg ctaagatcac
     2341 tgagatctac gagggtacaa gcgaagtcca gcgtatggta atctccggtg cactgttaaa
     2401 gtaatttcga atcaatccgc gtaaaattta aacatataaa acataaggag tgaaaaatac
     2461 atgaatatcg tagtatgtat taaacaggtt cctgatacaa agggcggcgt taagttcaac
     2521 ccggatggaa cgcttgacag agcagcaatg cttgctatca tgaacccaga tgataaagct
     2581 ggtcttgaag cagcacttag aatcaaagat gagacaggag caaaagttac agttcttacc
     2641 atgggacttc caaaggctga tgcagttctt cgtgaagcat tagcaatggg tgctgatgaa
     2701 gcagttcttg taacagaccg cgtattaggt ggagctgata catgggcaac atctaccaca
     2761 atcgctggtg cacttcgtaa cttagactat gacttgatca tcacaggacg tcaggctatc
     2821 gatggtgata ctgcacaggt tggtcctcag atcgctgagc atcttggact tccggttatc
     2881 tcttacgcag cagacatcaa agtagagggt gattctgttg ttgtaaaacg tcagtacgaa
     2941 gacagatatc atgagttaaa agcaaaaatg ccttgtctgg taactgctct ttctgaatta
     3001 aatgagccgc gttacatgac accgggcgga atcttcgatg catttgacaa agaagtaacc
     3061 gtttggggca gagcagactt aaaggatgtt gacgattctg atcttggttt aaagggatct
     3121 cctactaaga ttgcaaaagc atctgataaa gttgcaaagg gtgctggtga gaaagtaaac
     3181 cttgatccgg cagaatccgt agcatatttg atcggtaaat tcaaagagaa acacattatc
     3241 taataaaaaa aggaaaagtg gtgaataaca tggcattaga agaatataaa ggagtatttg
     3301 tctttgcaca gcaggtagat aacgtattag atggcgttgc atttgagtta cttggaaaag
     3361 gcaaagactt agcaaaagat ttaggtacag acgtaactgc tgtattaatt ggttctggtg
     3421 taaaaggatt agcagatcag ttagctgagt atggtgctga caaagttatc gtagtagatg
     3481 atcctgaatt aaaagactac agaacagagc cgtatgctca tgcattagca tctgtaatta
     3541 acgagttcaa acctgaaatc atgttagttg gtgcaactgc tatcggccgt gacttaggac
     3601 ctactgtttc tgcaagagta aaaacaggtc tgacagcaga ctgtacatct cttgagatcg
     3661 gtgatttccc actggttgct gcacctggta aagaatcaga gcagaaacac aaccagttat
     3721 taatgacacg tcctgcattt ggtggtaaca ccatcgcaac aattgcctgc ccggacaacc
     3781 gtcctcagat ggcaactgta cgtccaggtg ttatgcagaa gatcgctccg atcagcggtg
     3841 caaaagcaaa cgttgtagag tacaacccgg gatttacacc aaacaacaga tatgttgaga
     3901 tcttaaacat cgtaaaagct gttaagagca cagcaaacat tatggaagca aagatccttg
     3961 tttccggtgg tcgtggagtt ggttccaaag agaacttcaa acttttggaa gatcttgcag
     4021 aggtacttgg cggtactgta agctgctccc gtgcagttgt agagaatggc tggttaccag
     4081 ttgatttaca ggtaggtcag actggtaaaa ctgttcgtcc tcagatctac tttgcaatcg
     4141 gtatttccgg agcaatccag cacgtagctg gtatggaaga ttctgacctg atcatcgcaa
     4201 tcaacaaaga tgaagatgct ccaatcttcg acgtagctga ttacggtctg gttggtgatc
     4261 tgaataagat cgttcctgct ttaacaacag cattaaaagc tgagttaggc aaataataca
     4321 taggtgaata acctgtaaga tagaaagccc gtctttcgtg tgcggcttct gtgaggaagc
     4381 tgacaggaag gacgggcttt ttgctgggag ctggagaaaa tttgaggctt cttgtttgga
     4441 gttggaggaa atttgaggct cttggtcagg agttgaggga aaatatactt agttgtttgt
     4501 caaaatcgtt atggtacagt ttttctgatc ctcagatacg gagtcgaccg tgatcatata
     4561 cccggtatca atggtcaact gtccgttgtt gtcataccag ccaaagcctg tggcagtgcc
     4621 tccgatcacg tcaccgtccc ggtaaaagcc atttccgtta ttgaccagtc ttaagatccg
     4681 cctgccgtta ttggtggaat cgtagacagg actgtagtta tcagcggtca gatagggggc
     4741 accgtattcg tcgtttgcaa gggtggcatc gacgtgataa atacgaagtc cgccggaaga
     4801 aaacagaccg tcataattac cattcggggt gatatagtca aggatcatgt attctgaaaa
     4861 ataactggaa gcatcagtgc gcggtatgat cagacagctt ccttaatttt tcgccatcta
     4921 aatccggcag ggtggaagcc caccaggatg ccgtacagcc ataccagaaa tcagcattgc
     4981 ctccgcttgg aacagaaagg cagagagcgt cgatatagcc atcgccgtca gaatcataat
     5041 tggtatagtc gatcgtatca tttaaaccgg aaagaacctc acgcaccagc gtttcgtatc
     5101 catcgttcgt tttttcgtag gatgcgatcg tacctttcgc tgtgtaatga aatacatttc
     5161 cggtcatatg cagtgatccg tatgaagcac ggtcaaaata actagtcagg ctctcacagg
     5221 gataaaattc agtattgtct gtgccaaaca gataggagga aatttcttca tcggacaggc
     5281 gtctgtctgt atcatatttc gcatccgcaa aatcgaccac aacaacaagt gctcggacat
     5341 tgcctgtcgt cggcagaata tctgaggcga gagctgtaat cggtggcggc gtcattggcg
     5401 tgtgaagctg cccgtatgcc gcccccaaaa gcagggcacc aaggcagata accggaacgg
     5461 tgttaagtac attttttttc tgtatcatgg taaaaactc
//
