LOCUS       c00215_gnlPro..         4288 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PFOBJKDN_215.
ACCESSION   c00215_gnlPro..
VERSION     c00215_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 23:01:28
            Original ID  :: gnl|Prokka|PFOBJKDN_215
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4288
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4288
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[318:4287](+)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      319..4287
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..4288
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4288
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(132..242)
                     /locus_tag="ctg215_1"
                     /transl_table=11
                     /translation="MPGKSQAKYIIRHDFTKDNVQIGEKYKLFMNKISLD"
     CDS             319..4287
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_2"
                     /sec_met_domain="HGD-D (E-value: 4.1e-61, bitscore: 194.5,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 2.3e-39, bitscore:
                     122.5, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENNTLHKLGIDIGSTTVKVAVLDEHDNLLFSDYERHFANIRETL
                     SSLIQKAFDQLGDRKVAPMITGSGGLTLAKHLDVPFVQEVISVSTALQHYAPQTDVAIE
                     LGGEDAKIIYFEGGNVEQRMNGICAGGTGSFIDQMASLIQTDASGLNEYAKNYKAIYPI
                     AARCGVFAKTDIQPLINEGATREDLSASIFQAVVNQTISGLACGKPIRGHVAFLGGPLH
                     FLSELKVAFIRTLNLDDEHAITPDNSHLYAAIGSALNCKPEQNTTLSSMLEKLNSDIHM
                     EFEVARLDPLFKDQAEYDTFRARHDGHNVKTGDLSTYEGNCYLGIDAGSTTTKIALVGE
                     DGSLLYSFYSNNNGSPLSTAIRSIKEIYEKLPANAHIVHSCSTGYGEALLKAALMLDDG
                     EVETVAHYYAAAFFDPEVDCILDIGGQDMKCIKIKNQTVDSVQLNEACSSGCGSFIETF
                     AKSLNFSVQDFAKEALFAKNPIDLGTRCTVFMNSKVKQAQKEGASVADISAGLAYSVIK
                     NALFKVIKLSDASELGKNIVVQGGTFYNDAVLRSFEKISGCECVRPDIAGIMGAFGAAL
                     IARERHEADYQTTMLSIDEINALTFDTKLARCQGCTNHCLLTINRFSGNRQYITGNRCE
                     RGVGGVKNKENIPNLFEYKNKRLFDYPSLKPEEALRGTVGIPRVLNMYENFPFWATFFK
                     KLGFSVVLSPQSTRKIYELGIDSIPSESECYPAKLAHGHVTWLIHQNVDFIFYPCVPYE
                     RNEFPDSNNHYNCPIVTSYAENIKNNVEDITSGKVRFYNPFMAFTSEETLSSQLVKTFG
                     APEFAIPESEIRDAVSEGYKELAVVRMEMQKKGEEVLAYMEKTGKRGIVLAGRPYHVDP
                     EINHGIPELINSYGIAVLTEDSVSHLHQVERPLIVMDQWMYHSRLYAAANFVKTRDDLD
                     LIQLNSFGCGLDAVTTDAVNDILTKSGKIYTCLKIDEVNNLGAARIRIRSLLSAIRVRE
                     KKQTKRTIIPSNYNRVVFTEEMRKNYTILCPQMSPIHFELLEPAFCAAGYNLVVPDVPS
                     RTCVDVGLKYVNNDACYPSLIVVGQLMSAVMSGNYDMNKTAILISQTGGGCRASNYIGF
                     IRRALEKAGYPNVPVISINLSGLESNPGFKLTPSLIQHGLYALEFGDIFLRCLYRTRPY
                     EAVKGSANALHEKWKKEVISFITQDKLLSHRKFKQMCRQIIRDFDNLDRVDVKKPRVGV
                     VGEILVKFHPAANNDLVGLLESEGAEAVVPDLTDFLLYCFYNTGFKADNLGFSKKSKRI
                     GRLGINFFEWLRSAARDEFEKSKHFDPPAHIDDLANYAR"
ORIGIN
        1 atataataag gtttggaaca gcgtaacgac caattttacg ttctaattta tttaaccagt
       61 tcatcggata aaaccctaac ctttctttga aaacactact tttttcagta tatccgtatg
      121 cctgtgcttt gtcaatccag gctgatttta ttcataaaaa gtttatattt ttccccgatt
      181 tgcacattgt cttttgtaaa atcatgtcgt ataatgtact ttgcttggct tttgcccggc
      241 attcttttta ataagaagaa acagccactt tatttatata ttatccggat tttccggagt
      301 tactgactgg aggaaattat ggaaaataat acgcttcaca aacttggaat tgatatcggt
      361 tctaccaccg taaaggttgc tgtgctcgac gagcatgaca atctgctctt ttccgattat
      421 gaaaggcatt ttgccaacat acgggaaacg ctttcttctc tgatccagaa agcattcgat
      481 cagctcggtg accggaaggt tgctccaatg atcaccggtt ccggcggact tacacttgca
      541 aagcacttag atgttccttt cgtacaggaa gttatctctg tgtctaccgc cttacagcac
      601 tatgctccac agacagacgt tgcgatcgag ctcggcggtg aagacgcaaa aattatttat
      661 tttgagggcg gtaacgttga acagcgtatg aacggtatct gcgcaggcgg taccggttct
      721 tttattgacc agatggcttc cctgattcag acagatgcct ccggtctgaa tgaatatgcg
      781 aaaaattaca aagcaatcta cccgattgca gcccgctgcg gtgtttttgc caaaacagac
      841 atccagcctc tgatcaacga aggtgccaca agagaggatc tctctgcttc tattttccag
      901 gcggtggtaa accagacgat cagtggtctt gcctgtggta agccgatccg cggccacgtt
      961 gcattcttag gtggtccgct ccacttttta tctgagttaa aagttgcatt tatccgcacc
     1021 ttaaacttag atgatgagca tgcgatcacg ccggacaatt cgcaccttta tgcagcgatc
     1081 ggttctgcct taaactgcaa accggaacag aataccactc tctcttccat gcttgagaaa
     1141 ttaaactctg atatacatat ggaattcgag gtagcacgtc ttgatccgtt atttaaagat
     1201 caggcagaat acgatacctt ccgtgcccgc cacgacggac acaatgtaaa gacaggtgat
     1261 ctttccacct acgaaggcaa ctgttatctc ggtatcgacg ccggttccac aacaacaaag
     1321 atcgctctcg tcggcgagga cggttccctg ttgtattcat tttacagcaa caacaacgga
     1381 agcccgcttt ccactgcgat ccgttccatc aaggagatct atgaaaaact tccggcaaat
     1441 gcacatatcg tacactcctg ctctaccggt tacggtgaag ctctcttaaa agcagcgctg
     1501 atgttagacg atggtgaagt agaaacggta gcacattatt atgcagcggc attttttgat
     1561 cctgaggtag actgtatctt agacatcggt ggtcaggata tgaaatgtat caagatcaag
     1621 aaccagacgg tagacagtgt ccagttaaac gaagcctgtt cttccggctg tggttctttt
     1681 atcgaaacat ttgcaaaatc cttaaacttt tctgtacagg acttcgcaaa agaagcttta
     1741 tttgccaaaa acccgattga tcttggaaca cgctgtactg tattcatgaa ctctaaagta
     1801 aagcaggcac agaaagaagg agcttccgta gcagatatct ctgccggact tgcctactcc
     1861 gtcattaaaa atgctttatt taaggttatc aaactctccg atgcaagtga gctcggaaaa
     1921 aatatcgtcg tacagggtgg tactttttac aatgatgccg tgctccgcag ttttgaaaag
     1981 atttccggct gtgaatgtgt ccgtccggat atcgcaggta tcatgggtgc tttcggagct
     2041 gctctcatcg caagagaacg acatgaagct gactatcaga caacgatgct ttccatcgac
     2101 gagatcaatg cattaacttt tgataccaaa cttgcaagat gtcagggttg taccaaccac
     2161 tgtctgctga cgatcaaccg tttctccgga aaccgccagt atatcacggg aaaccgctgt
     2221 gagcgcggtg tcggcggcgt aaaaaacaaa gaaaatattc cgaacttatt tgaatacaag
     2281 aataaacgct tattcgacta cccttcctta aagccggaag aagccctgcg tggaacagta
     2341 ggtatcccaa gagttttaaa tatgtacgag aatttcccgt tctgggctac ctttttcaaa
     2401 aaactcggat tttccgttgt actgtcacca cagtccacca gaaaaatcta tgagctcggc
     2461 attgactcaa ttccaagtga atccgagtgt tatccggcaa aacttgcaca tggacatgtg
     2521 acctggctga tccatcagaa tgtagacttt attttctacc catgtgtacc ttatgagcgc
     2581 aatgaatttc cggattccaa caaccattac aactgcccga tcgtaacctc ctacgcggaa
     2641 aatatcaaaa ataacgtgga agatatcact tccggcaagg tacgttttta taatccattc
     2701 atggcattta ccagcgaaga aactttatct tcccagcttg taaaaacctt tggtgcaccg
     2761 gaatttgcca tcccggaatc agagatccgt gacgctgttt ccgaaggtta caaagaactt
     2821 gctgttgtcc gcatggaaat gcagaaaaaa ggtgaagaag tcttagctta tatggagaaa
     2881 accggcaaac gcggtattgt attagcagga cgtccatacc atgttgaccc tgagatcaac
     2941 cacggtatcc cggaactgat caattcctat ggtattgccg tactgactga ggactccgtt
     3001 tctcaccttc atcaagtgga acgccctctg atcgttatgg atcagtggat gtatcattcc
     3061 cgtctgtatg cggctgcaaa ctttgtaaaa accagggatg accttgatct gatccagttg
     3121 aactccttcg gttgtggctt agacgcagtt accaccgacg cagtcaacga catcctgaca
     3181 aaatcaggca agatctacac atgcttaaag attgatgagg ttaacaactt aggtgctgca
     3241 aggattcgta tccgttccct gctctccgcc atccgtgtgc gtgagaagaa acagacaaaa
     3301 cggacaatta ttccttccaa ctataacaga gttgttttca cggaagaaat gcgtaaaaat
     3361 tatacaattc tctgtccgca gatgtcaccg atccactttg agctgttaga acctgcattc
     3421 tgtgcagccg gttataacct ggtggtaccg gatgttccaa gccgcacctg tgtggatgtc
     3481 ggattaaaat atgtaaataa tgatgcctgc tatccgtcac ttattgtggt cggacagctt
     3541 atgagtgctg ttatgtccgg aaattatgac atgaacaaaa ccgcgatcct gatctcgcag
     3601 accggcggag gctgtcgtgc cagcaactac atcggtttta tccgtcgtgc tcttgaaaaa
     3661 gcaggatatc cgaatgtacc ggtaatctcc atcaacttaa gcggacttga aagcaatcct
     3721 ggctttaaac tgacgccttc actgatccag cacggactat atgcattaga gtttggcgat
     3781 attttccttc gctgtctgta ccgcacacgt ccttatgaag cagtaaaagg atctgcgaat
     3841 gcactgcatg agaaatggaa aaaagaagta atctccttta tcacacaaga taaactgttg
     3901 tcccaccgca aatttaagca gatgtgccgt cagatcatcc gcgattttga caaccttgac
     3961 cgtgtagatg tgaaaaaacc gcgtgtcggc gtcgttggag agatcttagt aaaattccat
     4021 cctgctgcaa acaacgattt agtcggattg ttagaatccg aaggcgcaga agctgtcgtg
     4081 ccggatctga ccgacttcct gctctactgc ttctataaca ccggatttaa ggctgacaac
     4141 ctcggtttca gcaaaaagtc caaacgcatt ggacgacttg gcatcaattt ctttgaatgg
     4201 ttaagaagcg ctgccagaga tgaatttgaa aagagtaagc attttgatcc gcctgctcat
     4261 atcgacgatc ttgcaaatta tgcaagag
//
