LOCUS       c00350_gnlPro..         3171 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PFOBJKDN_350.
ACCESSION   c00350_gnlPro..
VERSION     c00350_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 23:01:28
            Original ID  :: gnl|Prokka|PFOBJKDN_350
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3171
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3171
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[20:2501]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      21..2501
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..3171
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3171
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             21..1280
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Epimerase"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg350_1"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 8.6e-45, bitscore:
                     141.5, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 5.7e-36,
                     bitscore: 111.4, seeds: 81, tool: rule-based-clusters)"
                     /sec_met_domain="Epimerase (E-value: 2.5e-08, bitscore:
                     21.3, seeds: 96, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRLLIIGCGGVAGVAIHKCCQNSKTFTEICIASRTKSKCDALKE
                     KLEKTTDTVITTAQVDADNTEELIALIKKYQPDAVLNVALPYQDLTIMDACLACKVDYI
                     DTANYEPEDTDDKEWRAIYEKRCKEEGFTAYFDYSWQWAYREKFKEAGITALLGSGFDP
                     GVTSVFTAYALKHYFDEIETIDILDCNGGDHGYAFATNFNPEINLREVSANGSYWENGH
                     WVETKPMEIKREYNFPQVGEKDMYLLHHEEIESLAKNVPGVKRIRFFMTFGQSYLTHMK
                     CLENVGMLSTSPIEYEGREIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGKKDGKEK
                     TIYIYNVCDHQECYKEVGSQAISYTTGVPAMIGAALVVDKVWDKDGVFNIEEFDPDPFM
                     DMLNQYGLPWVVDENPQTVA"
     CDS             1368..2501
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Orn_DAP_Arg_deC"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg350_2"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 8.5e-15,
                     bitscore: 42.7, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKWQEIPTPCYVVDEKKLKQNLKILQNLEQETGCHVLLAQKAFSM
                     FSLYPLIGQYISGTTASGIFEARLGKEEMGKENHVFAPAYKEKDMEELVQICDHVIFNS
                     FAQYEKYQHYFLQQECTASAGIRVNPECSTQEGHEIYDPCGPGSRLGVVKSEFPDQLPE
                     GIEGLHFHTLCEQNADDLITTWHAFEEKFGSYLKQVKWLNLGGGHHITRADYQLDELKK
                     LICEIRRKYNVRVYLEPGEAIALNAGYLVTEVMDIVHNGMDILILDASAACHMPDVLEM
                     PYRPPLKDGYLPGEKPHTCRLSSMTCLAGDVIGDYSFAQKKQVGDRLVFEDMAIYSMVK
                     NNTFNGMPLPGIAVLREDGDVEMIKEFGYEDFKGRLS"
     CDS             2473..3171
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PAD_porph"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg350_3"
                     /sec_met_domain="PAD_porph (E-value: 9e-56, bitscore:
                     177.1, seeds: 436, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISKADCPEMIKDTTPVQDGFFMPGEFEQHQGCIMIWPERPGSW
                     RNGAKEAETAFSAVIRAIAASETVYVAAGKKGIDHAKAFAESLKKEENLHPVVVFEMET
                     DDSWARDVAPTFLVNRTDGKISDLTAAGNGTVQTGDDVKNSQVRGVNWSFNAWGGEVDG
                     LYASYEKDNAFAWNFCRKFEFDCYDAEPFVLEGGSIHSDGEGTLLVTESCLLSAGRNPS
                     LTKEQIEEQLK"
ORIGIN
        1 gcgcaggaaa ggagattaaa atgggaagat tattaattat cggatgtggc ggagttgcag
       61 gagttgcgat tcacaaatgc tgccagaaca gcaaaacttt tacggagatc tgcattgcga
      121 gcagaacaaa atcgaaatgt gacgccttaa aagagaaact ggaaaaaaca acggacactg
      181 tcatcacaac agcacaggtg gatgcggaca atacggagga actgatcgcc ctgatcaaaa
      241 aatatcagcc ggatgctgtg ttaaatgttg ctttgccata tcaggattta acaattatgg
      301 atgcctgcct tgcatgcaaa gtagactata tcgataccgc aaattatgag ccggaggata
      361 cggatgacaa agaatggcgt gccatctatg aaaaacgctg taaagaagag ggatttacag
      421 catattttga ctattcctgg cagtgggcat accgtgaaaa attcaaagag gcaggaatta
      481 cggcattgct tggaagcggg tttgatccgg gagtgaccag tgtatttacc gcttatgccc
      541 tgaaacatta tttcgatgag atcgagacga ttgatatctt agactgtaac ggcggcgacc
      601 acggctatgc ctttgccacc aactttaacc cggagatcaa cctgcgtgag gtttccgcaa
      661 acggttctta ctgggagaat ggtcactggg tagaaacaaa accaatggaa attaaaagag
      721 aatataattt cccacaggtt ggcgagaaag atatgtatct gctgcatcat gaggaaattg
      781 agtcccttgc aaaaaatgta cctggagtaa agcggatccg tttctttatg acctttggac
      841 agagctatct gacacatatg aaatgtttag aaaatgtcgg aatgctctca acatcaccga
      901 tcgagtatga gggcagggag atcgtaccaa tccagttctt aaaagcactg cttccggatc
      961 cggcatcttt aggaccgcgc acggttggaa aaacaaatat cggctgtatc tttacgggaa
     1021 aaaaagacgg aaaagaaaag acgatttata tctataatgt ctgtgaccat caggaatgtt
     1081 acaaagaagt cggttcccag gcaatttcct acacgaccgg tgttccggct atgatcggtg
     1141 cagcgttagt tgtcgataaa gtctgggaca aagacggtgt attcaatatt gaagagtttg
     1201 atccggatcc gtttatggat atgttaaacc agtatggtct gccatgggtt gtggacgaaa
     1261 atccgcagac ggtggcataa aataagaata tgtgtgaaac gggttggata aaattttaaa
     1321 tttgccagct tttgcgcagc ggatttttgc agaaatggag aattatcatg aaatggcagg
     1381 agattcctac cccgtgttat gtggtagatg aaaagaagct aaaacaaaat ttaaaaatat
     1441 tgcagaattt ggagcaggaa accggctgcc atgtgcttct ggcacagaag gcattttcca
     1501 tgttttcact gtatccgctg atcgggcagt atatcagcgg aacgacggcg agcggaatct
     1561 ttgaggcgcg ccttggaaaa gaggagatgg gaaaggaaaa tcatgtattt gcaccggctt
     1621 ataaggaaaa agatatggaa gaactggtgc agatctgcga tcacgttatt tttaattcct
     1681 ttgcgcagta tgaaaagtat cagcactatt ttttgcagca ggagtgcact gcaagtgccg
     1741 ggatccgcgt gaacccggag tgctccacac aggaagggca tgagatctat gatccatgcg
     1801 gaccgggatc aaggcttggt gtggttaaaa gtgaatttcc ggatcaactg ccggagggaa
     1861 tcgaaggatt acattttcat acactctgcg agcagaatgc ggatgatctg atcacgacct
     1921 ggcatgcgtt tgaggagaag tttggcagtt atttaaaaca ggtaaaatgg ctgaacttag
     1981 gcggcggtca tcatattaca agagcagact atcagttgga tgaattaaag aagttaatct
     2041 gtgagatcag gcgaaaatat aatgtccggg tctacttgga accgggtgag gcgatcgcat
     2101 taaatgcggg ctatcttgtc acggaggtta tggatatcgt acacaatgga atggatatcc
     2161 tgattctgga tgcatcggcg gcatgccaca tgccggatgt gcttgaaatg ccatatagac
     2221 cgccgctaaa agacggttat ctgccgggcg aaaagccgca tacctgccgg ctttcttcca
     2281 tgacctgcct tgccggggat gtgatcggtg attacagctt tgcacagaaa aaacaggtgg
     2341 gagaccgtct ggtctttgag gatatggcaa tttattccat ggtcaaaaac aatacattta
     2401 acgggatgcc gctgcccgga attgcagtct tacgggaaga tggagatgtc gagatgataa
     2461 aggagtttgg ttatgaagat ttcaaaggca gactgtcctg agatgataaa agatacaaca
     2521 ccggtacagg acggattttt catgccgggt gagtttgagc agcatcaggg atgtatcatg
     2581 atctggccgg agcgtcccgg ttcctggaga aatggcgcga aggaagcaga aacagcgttt
     2641 tcagctgtga tccgtgcaat cgccgcgagt gagacagtgt atgtcgctgc cggaaaaaag
     2701 gggattgacc atgcaaaagc atttgcggaa agtctgaaaa aggaagaaaa cttacatccg
     2761 gtggttgtct ttgagatgga gaccgatgat tcctgggcgc gggatgttgc gccgaccttt
     2821 ctggtaaaca gaacggatgg aaagatttca gacctgacag cagcaggaaa tggtacggtg
     2881 cagacaggtg atgatgtaaa aaactcacaa gtgcgcggtg taaactggtc ttttaatgcc
     2941 tggggcggtg aagtggatgg actatatgct tcttatgaaa aagacaatgc gtttgcatgg
     3001 aatttctgca gaaaattcga gtttgactgt tatgatgccg aaccgtttgt gttagagggc
     3061 ggttctatcc acagcgacgg agagggaaca ttgcttgtca cggagtcctg tctgctcagt
     3121 gcggggagaa acccatcgct tacaaaggag cagattgaag aacagttaaa g
//
