LOCUS       c00256_gnlPro..         3434 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KPKPEKPM_256.
ACCESSION   c00256_gnlPro..
VERSION     c00256_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 22:11:42
            Original ID  :: gnl|Prokka|KPKPEKPM_256
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3434
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3434
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[73:3072]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      74..3072
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3434
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3434
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             74..1213
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg256_1"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.7e-167, bitscore:
                     545.0, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVEREKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFERMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLXXXXXXXXXXXXXXSG"
     CDS             1281..2333
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg256_2"
                     /sec_met_domain="Gly_radical (E-value: 4.2e-46, bitscore:
                     144.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.5e-93, bitscore:
                     301.6, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPDYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGR
                     DEKSGVQVAPRFEPITSEYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAF
                     EMGLHDGDVERIRATGIAGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGN
                     NDDRTDSIAVDITEKFMEYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGT
                     PFAPGANPMNGRDTKGAVAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQVNNLV
                     SLLDGYFTPDGGQHLNVNVFDQDLLLHAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLD
                     VISRTINNSL"
     CDS             2350..3072
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.4e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg256_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-36, bitscore:
                     114.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGITV"
     CDS             complement(3075..3380)
                     /locus_tag="ctg256_4"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCNACLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCEGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
ORIGIN
        1 tttacaatcc ttttcagata gggggaaaag acgtatcttt gcgtgcctaa accgaaatag
       61 acaagataag cttatgaagt ttgaaaacga gaactgggcg cagttcaaag gagaaacctg
      121 gaaaaaggag atcaatgtaa gagactttat ccagaacaat tacaaacctt ataacggaga
      181 cgatacgttc ctcgtaccct cttcggagaa aacgaagaaa gtgtggaaca aactaaccga
      241 gatgtttaag gttgaaaggg agaaaggagt atatgatgcc gaaacgaagc ttccccaggg
      301 gattgacaca tacggacccg gttacatcga taaggaaaac gaagtaatcg tcggcttgca
      361 gacagacgcc ccgttgaagc gcggtatctt cccgaaaggc ggtatccgca tggtcgaaaa
      421 cagtctggaa gcctatggct atcatctgga tccgatgaca aaagaaatct tcaccaaata
      481 cagaaagacg cacaacgaag gcgttttttc agcctataca gacgagatga ttgcggcacg
      541 tcgttccgcc atcattaccg ggttgcctga tgcctacgga cgcggacgta tcatcggtga
      601 ttaccgtcgt gtacctctgt acggaaccca gatcctgatc gaagaaaaga aacgtttcca
      661 gaaacgtctc gatatacagg aactgacaga agaaatcatt cagagccgcg aggagataac
      721 ggagcagatc aaagcgctga aagcatttga gagaatgtgt gccggttatg gtttcgatgt
      781 gacacgtccg gcaaaggatg cccgcgaagc cgtgcagttc ttgtatctgg cttacctggc
      841 agccgtaaag gatcaggatg gagcagccat gtctctcgga cgtacttcta ctttcctcga
      901 tatctatatc gaaaaagata tttgcgaagg taagctgacg gaagaagagg cacaggaatt
      961 gatcgaccag tttattatca aactgcgtat tgtccgtttc ctgcgtactc cggaatacaa
     1021 cgacctgttc tccggcgacc ctgtttgggt gactgaatcg ctgggcggac aaggtgttga
     1081 cggccgttcg ctggtgacaa agacatctta ccgttatctg cataccttgt ataacctcgg
     1141 cccggcaccc gaacccaacc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nngctccgga taactggaaa aacttctgcg caaaagtatc gatcgatacc tctgccatcc
     1261 agtacgagaa cgacgatctg atgcgtccgg actatggcga cgattacggt atcgcctgct
     1321 gtgtgtctcc gatgaaaatc ggcaagcaga tgcagtattt cggagctcgt gccaacctgg
     1381 caaaatgctt gttgtatgcg atcaatggcg gacgtgacga gaaatcgggt gtgcaggtgg
     1441 ctccccgttt cgaaccgatc accagcgagt atctggatta caacgaagtg atggagaaat
     1501 acgagcagat gatgcgctgg ttggcaaaag tctatgtcaa tgcactgaag atcattcact
     1561 acatgcacga caaatatgcg tatgaagcat tcgagatggg gcttcatgac ggagatgtgg
     1621 aacgtattcg cgcaaccggt atcgccggcc tttctatcgt cgccgactcg ctggctgcca
     1681 tccgtgatac gaaagtgaaa gtgatccgtg acgaacgtgg tttggctgtc gacttcgaac
     1741 gcgaaggcga atatgtacct ttcggaaaca atgacgaccg cacggacagt attgctgtcg
     1801 acattaccga gaagtttatg gaatatctgc gccagcatca gacataccgc aaggcaactc
     1861 cgacacaatc tatcctgacg atcacatcaa acgtggtcta cggaaagaaa accggaacga
     1921 ctcccgacgg ccgtccgggc ggtacgccgt ttgctccggg agccaacccg atgaacggac
     1981 gcgatacgaa aggggctgtc gccgctttgg cttctgtagc caaactgcct tttcagcatg
     2041 cgcatgacgg tatttcctat acattcgccg tttctcctgc aacgttgggt aaggaacgcg
     2101 atatccaggt gaacaactta gtgagcctct tagatggtta tttcacgccc gatggcggcc
     2161 agcatctgaa tgtgaacgta ttcgatcaag acttgctgtt acatgcgatg gaacatccgg
     2221 agaaataccc gcagcttacg atccgtgttt cgggctatgc cgtcaacttc gttaagctga
     2281 cacgcgaaca gcaactcgac gttatctcgc gcaccatcaa caacagttta taattgtcca
     2341 ttgattaata tgttggggcg tatacattct ttagagagtt tcgggaccgt tgacggtccc
     2401 ggaattcgct ttgtcgtttt tatgcaggga tgtccgcttc gttgtctgta ttgccacaat
     2461 ccggatacct gggaggtaaa gcgtgaaacg ccttatctgc tggaacctga agcgttgctt
     2521 gccgaagtgc tgcgctataa gaattttatc gctaaaggtg gtgttaccgt gaccggtggc
     2581 gagcctttgt tacaacctga attccttaaa gagtttttcc gcctttgccg tgagaacggt
     2641 attcatacgg cgttggacac ttccggctat atctgttccg gtaaagcatt ggaggtgctc
     2701 gaacaggtcg atctggttct gttagatatc aagacgatcg acgccggtct tcatccccgt
     2761 ttgacggccg taaaactcga taatacttta cgctttcttg atgaactgga gaaacgcggc
     2821 atccctgtat ggattcgcca cgtgatcgtg cccggcctga cggacgatga tgaagccttg
     2881 agcaagctgg ccgaatatat cagttcatat aacgtggtcc agaaagccga acttctgcct
     2941 tatcatacaa tgggagctta caaatatgag gcacaaggcc tggactataa gctgaaaggt
     3001 gtggaaccgc tttcagcaga gcgtctggcc aatgcgaaag cgatctttat gaaacacggg
     3061 ataaccgtat aagcctaatc gagtgtctgc tctttgatga tgatcggttt acggctgatc
     3121 cggttgactg tgcggacact gtcgctttca atactggtaa catctccctc gcagttggtg
     3181 aaatagatgt aatgtcccat atccatgaaa cgatagacct tacacccttc gtgttcgaac
     3241 agatattcga cttgataagt ggaattgttt accggttttt cctttgtaag gggtatttgt
     3301 gagacacatg aagaaagaca tgcgttgcaa acgagcaata gacttgctga aaatgtatta
     3361 tacaaactag ctcttctcat cttcgaatat ttttaagtta acatccaaag ttaacaatta
     3421 aagaatcaca tggt
//
