LOCUS       c00269_gnlPro..         3397 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CEDCOPAD_269.
ACCESSION   c00269_gnlPro..
VERSION     c00269_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:48:52
            Original ID  :: gnl|Prokka|CEDCOPAD_269
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3397
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3397
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[387:3395]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      388..3395
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3397
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3397
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             388..2616
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg269_1"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-48, bitscore:
                     151.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEMNKVFKDGLWSKEINVSDFVHTNITPYEGDASFLAGPTERTKK
                     VWNECLKALEEERANNGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKP
                     YGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLHNITSPMTDARIRLREEVAEQIKALKEIK
                     ILGEYYGLDLGRPATNAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDH
                     GKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLL
                     NFEEVWSNYKKVLMQIARVYNDAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENAYPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDAQDGISNTFSIVPKSLGVDQETRIENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFIKLSREHQLEVISRSFHERM"
     CDS             2685..3395
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 77.9;
                     E-value: 3.1e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg269_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.5e-29, bitscore:
                     89.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGE
                     STETTIDEIVRMAVSQKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQSIHICIDTN
                     GSIWNEEVKELLQWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAGWLEKNGKPFWLRY
                     VLVPGYSSFEEDIRALGEHFKNYQMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTL
                     KQLETAKTLFGEYFN"
ORIGIN
        1 ccgatttata atagtaatag cataaaaaaa atcatttttt attttgcaga ttaaaaacaa
       61 acttctaact ttgcaccgtc ttagaaaaag aattaagact aactgatggc ggtatcgtct
      121 agtggcctag gacgcagccc tctcacggct gaaactcggg ttcgattccc ggttccgcta
      181 cttttagaag ctaattggtt tattgtcacc aattagcttt attttttatg gaattgttat
      241 aaagagtcct tattgttttt tcatttattg accactttat acttaaaaat gcacaagtat
      301 tcgcattata cctactaata gtgatgcata gctcacaaaa aaggagtacc tttgcagccc
      361 taaatctctt aataatggaa taacagtatg gaaatgaaca aagtttttaa ggatggattg
      421 tggagtaagg aaattaatgt ttcagacttc gtacatacca acattacccc ttatgagggc
      481 gatgcctcat ttctggcagg ccccacagag cgaactaaaa aagtgtggaa cgaatgcctc
      541 aaggcactag aagaagaacg tgccaataac ggagttcgtt cccttgataa tgtaacagta
      601 tctaccatca cctcacataa agcaggatac attgaccgtg aaaacgaact gattgtaggt
      661 cttcaaacgg atgaacttct gcgccgtgcc atcaaacctt atggtggtat caaagtcgta
      721 gaaaaagcct gccatgaaaa cggtgtggaa gtagatgaaa aagtaaagga tatctttact
      781 cattatcgca aaacccataa tgatggcgta tttgatgctt acaccgaaga aatccgctct
      841 ttccgttcac tcggtttcct gaccggtctg cccgataatt atgcacgcgg gcgtatcatc
      901 ggtgactacc gccgtttggc actgtatggt atcgaccgac tgattgaagc aaaacaagaa
      961 gatttgcaca atatcaccag tccgatgacc gatgcccgta tccgcctgcg cgaagaagta
     1021 gccgaacaaa tcaaagcact gaaagaaata aagatattgg gtgaatatta cggactcgac
     1081 ttgggtcgtc ccgctactaa tgcacaagaa gctgttcagt gggtttatat ggcatatctt
     1141 gctgccgtga aagaacaaga tggagcagct atgtcattgg gcaacgtttc ttcattcctg
     1201 gatatctata ttcaatatga tttggatcat ggtaaaattg acgagtcatt tgcgcaggaa
     1261 ctggtagacc agttcattat caagttgcgt atggtccgtc atctgcgtat gcaatcatac
     1321 aacgatatct ttgcaggtga tcccacctgg gtaaccgagt ctatcggcgg acgcttcaac
     1381 gacggacgta ccaaggttac aaaaacttcc ttccgtttct tgcagacact atacaatctg
     1441 ggtccgtcac ccgaacctaa tatgactgta ctgtggagtc cggaattgcc ggaaggtttc
     1501 aaagaattct gtgcacaagt ttctatcgac acttcttcta tacaatacga aaacgacacg
     1561 ctgatgcgcg aagtgcgtaa ctgtgatgat tatggtatcg cctgctgcgt ttcttatcag
     1621 gctatcggca aacaaatcca gttcttcgga gcacgttgca atctggctaa agccctattg
     1681 ctggctatca atggcggacg ttgtgagaat acaggaactg taatggtgaa agatattcct
     1741 gtattgacag gggacttgct gaactttgaa gaagtatggt ctaattataa gaaagtatta
     1801 atgcaaattg cacgtgtgta taatgatgcg atgaatatca ttcactatat gcacgataaa
     1861 tattattatg aaaaggctca aatggcattc attgataccg atccacgtat caatctggca
     1921 tatggcgtag ccggtctgtc cattgccatt gactcattgt ctgccatcaa atacggcaaa
     1981 gtacgtgcca aacgtaatga tataggtttg accgaaggct ttgatattga aaatgcatat
     2041 ccttgcttcg gtaatgatga tgaccgcgta gaccacttag gggtagattt ggtttatttc
     2101 ttcagtgaag aattaaagaa acatccggta tacaaaaatg cccgccctac cctgtcactg
     2161 ctgactatca cctccaatgt aatgtacggc aagaaaacag gtgccactcc tgatggtcgt
     2221 gccaaaggag tggctttcgc tccgggtgcc aacccgatgc acggacgtga caagaacggt
     2281 gccatcgcct cattgagttc tgtagccaaa ctacgctatc gtgatgccca ggacggcatc
     2341 agtaacacgt tctctatcgt accaaaatca ctaggggtag accaggaaac ccgtatagag
     2401 aacctggtaa ccatgatgga cggttatttc accaaaggag cacatcatct gaatgtaaat
     2461 gtgctgaacc gtgaaatgtt ggaagatgca atggaacatc ctgaaaaata cccgcaacta
     2521 accatccgcg tatcaggtta tgccgtaaac ttcatcaaat tgagccgtga acatcagctg
     2581 gaagtaatca gccgttcttt ccacgaaaga atgtaacatc acatatacat tcatcgtagg
     2641 ggcgggtttt aaacctgccc ttaccagttt aaagacaaac cattatgatc agagtccatt
     2701 cttacgagtc aatggggacg tttgacggtc ccggtttacg cttagtcgta tttttacaag
     2761 gatgcaattt ccgatgcctc tattgtgcca accccgacac aattgacacc aaaggtgaaa
     2821 gtacggaaac aacaatagac gaaattgtgc gtatggccgt cagccaaaaa gcttttttcg
     2881 gtaaaaaagg aggtgtgact ttcagcggcg gcgagcctac attgcaagca aaagcactga
     2941 ttccgttatt ccagcgatta aaagaacaaa gcatccacat ctgtatagat accaacggca
     3001 gtatatggaa tgaagaagta aaggaactgc tacaatggac agacctcgta ttattagaca
     3061 tcaaggaatt taataatgtg cgccatcggc aactgacaga acgcagcaac gaacaaacca
     3121 tacgtaccgc cggatggctt gaaaaaaacg gaaagccttt ttggttgcgc tatgtattag
     3181 ttccaggcta tagttccttt gaggaagata tacgggcatt aggagagcac ttcaagaatt
     3241 atcaaatgat tcaaagagta gaaatactac cttatcatac attaggcgta cacaaatatg
     3301 aagccatggg aaaagaatac aaactaaacg gaataaaaga aaatactcta aaacaattgg
     3361 aaacagccaa aacgttattt ggtgaatatt tcaatac
//
