LOCUS       c00152_gnlPro..         6705 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NPLAGMNC_152.
ACCESSION   c00152_gnlPro..
VERSION     c00152_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 02:28:19
            Original ID  :: gnl|Prokka|NPLAGMNC_152
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6705
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6705
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3196:6176]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3197..6176
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6705
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6705
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             41..2770
                     /locus_tag="ctg152_1"
                     /transl_table=11
                     /translation="MNKRFSTLLAAALVAGSFSSAFAQLEGRNVEWKDAQASRAYYLAG
                     VHFVSPTDYLQAFSLQGLVNGADITAANPVAAAQWTVETKKVGTVTHYAFKNIEGEQYL
                     AFEKKDGAYALAAKKEANDETKSFRWFTVAANGNLQAVLSDEKEPYFLGLKNDGTADWS
                     LALLQSSEHHTDGSTSVIKKKVALYEIYNDQITAEAFASKFGENFTIEFPETLDDMDVF
                     ENVSVEKIANDNLGFKLVDNKGTKDTKDDEYVVLANERVSNSELAANAIGYRYAKMTAK
                     AIKDKKIDDKNTVFYAEENETNIGDGNYALVQKVKVGDLDNKDVTFGKVTNDETTRQLA
                     SVEYDCHSTFMGVTVGKGTRVANTEVANERMLIVNVTAKVADKKNSSALGLKNDFTGAR
                     WYDKVSEADFDQPQGQWLVAAGSAANTIKLINVQKPGFVQDNIRLYEAEGENTYTVFAS
                     DLGLSGLETITLTKVENYDKLNGYYSTLSDINQKFAFVTTGEYANEEAGSDLYLKAGVE
                     GDVVTVVPHAGSEWELIRNEKAQAVTANYKYYDGEKWVELNGKGNEEKGILPADTVRLA
                     YSYAINADELGLGTAWKMAKDDIEFFLKENGKGTYAIVYGATADEVKEYGVINGSEELV
                     AETTDFTNSTPFEIIDLDAAPSLAADSKHMTFEATNSIGFIASDENNNAVLAKETAALW
                     VDSVNAEGYATPKFFISYAGKMMTTAKKFKEIVDKAYANDEISKKEYAELTADENLYYK
                     SVERIKFVDAERLSGEDTLVIADEKYTGKDIEDFKFKVTENEDGDYVLKANGNYVYVIN
                     GNLVYGTDAKEAEAFVIEVTSAPTANEGIATSEVKVIAGEGNVTIAGAAGKKVVISNIL
                     GQVVANTVVSSDNATIAAPAGVVVVAVEGEAAVKAIVK"
     CDS             3197..5437
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg152_2"
                     /sec_met_domain="Gly_radical (E-value: 3.2e-46, bitscore:
                     144.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.3e-281, bitscore:
                     921.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVEREKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVGNSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFERMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFERESEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLVDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             5454..6176
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.4e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg152_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-36, bitscore:
                     114.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGVTV"
     CDS             complement(6179..6484)
                     /locus_tag="ctg152_4"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCNACLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCKGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
ORIGIN
        1 attcaatata catattatta ttaattaaac attttagatt atgaacaaga gattttctac
       61 tcttttggct gccgctttgg tagccggttc ttttagttca gctttcgcac agttggaggg
      121 gcgtaatgtt gaatggaagg atgcacaagc aagccgcgct tattatttgg caggggtgca
      181 ttttgtgagt ccgacagatt acttgcaggc tttttctttg caaggattag tgaatggtgc
      241 tgatattacg gctgcaaatc ctgttgctgc tgcacaatgg actgttgaaa ctaaaaaggt
      301 tggaactgtt acacattatg ctttcaaaaa tattgaagga gaacagtatc tggcttttga
      361 aaagaaagat ggagcttatg ctttagctgc caaaaaagag gcaaacgatg aaacaaagtc
      421 gtttcgttgg tttactgttg cagcaaacgg caatttgcaa gctgtgcttt ctgatgaaaa
      481 agaaccgtat tttttaggac tgaagaacga tggcacagca gattggtcat tggctttatt
      541 acaaagttct gagcatcata cggatggatc tacttccgtt atcaagaaga aggtagctct
      601 ctacgaaatc tacaacgacc aaattacagc agaagctttt gcgagcaagt ttggcgaaaa
      661 cttcacaatc gagttcccgg aaacattgga tgacatggat gtgtttgaaa atgtaagcgt
      721 agaaaaaatc gccaatgata atttgggttt caagttggtt gacaacaaag gcacaaaaga
      781 tacaaaagac gatgaatatg tagttttagc aaacgaacgt gtttccaact cagagttggc
      841 tgccaacgca atcggttatc gctatgcaaa aatgactgct aaagcgatta aagacaagaa
      901 aattgacgac aagaacactg tattctatgc tgaagagaac gaaacaaaca tcggagatgg
      961 taactatgca ttagttcaga aagtaaaagt tggtgacctg gacaacaaag atgtgacttt
     1021 cggtaaagtg acaaatgatg aaacaactcg tcagttggct tctgtcgaat atgactgcca
     1081 ttctactttc atgggggtta cagtaggcaa gggtacacgt gtcgctaata ctgaagttgc
     1141 caacgaaaga atgttgatcg taaatgtaac tgccaaagtt gcagacaaga agaattcttc
     1201 tgcactgggg ttgaagaatg atttcactgg cgcacgttgg tatgataaag tttctgaagc
     1261 tgacttcgat cagccacagg ggcagtggtt ggttgctgcg ggttcagctg ctaacacaat
     1321 taagttgatc aatgttcaga aaccgggttt tgtacaagac aatatccgtc tatatgaagc
     1381 agaaggtgaa aatacttaca cagtatttgc ttctgattta ggtttgtctg gtcttgaaac
     1441 aatcactttg acgaaagttg aaaattatga caagctgaac ggctactaca gtacattgtc
     1501 tgacatcaat cagaagttcg cctttgtaac aacaggtgaa tatgctaacg aagaagccgg
     1561 ttcagatctg tatttgaaag ctggtgtaga aggagatgtt gtaactgttg ttcctcatgc
     1621 tggttctgaa tgggaattga tccgtaacga aaaagcacag gctgttactg ccaactacaa
     1681 atattatgac ggtgaaaaat gggttgaact gaatggcaaa ggtaatgaag aaaaaggtat
     1741 cctgccggct gatacagttc gtctggctta ttcatatgca atcaatgctg atgaattagg
     1801 tttgggaaca gcttggaaga tggctaagga tgacatcgag ttcttcttga aagaaaatgg
     1861 taaaggtaca tacgctatcg tatatggcgc tactgctgat gaagtaaaag aatacggtgt
     1921 aatcaatggt tctgaggaat tagtagcaga aacaactgac tttactaact ctactccgtt
     1981 cgaaatcata gacttggatg ctgctccttc tttggctgct gacagcaagc acatgacatt
     2041 cgaagctact aatagcattg gcttcatcgc ttctgacgaa aataacaacg cagtattggc
     2101 taaagaaaca gctgctttgt gggttgactc tgtaaatgct gaaggttatg caactccgaa
     2161 gttcttcatt tcttacgctg gcaagatgat gacaactgct aagaagttta aagaaattgt
     2221 tgataaggct tatgctaatg atgaaatcag caagaaagaa tatgcagaac ttactgctga
     2281 tgaaaatctt tactacaaga gtgtagaacg tatcaaattt gtagacgctg aacgtctgtc
     2341 tggtgaagac actttggtta tcgccgatga aaagtatact ggcaaggata tcgaagactt
     2401 caagttcaaa gttactgaaa acgaagacgg tgactatgta ctgaaagcta atggtaacta
     2461 tgtatatgta atcaacggta acttggttta cggtacagat gctaaggaag ctgaagcatt
     2521 tgtaatcgaa gtaacttctg ctcctacagc caacgaaggc atcgcaacat ctgaagtgaa
     2581 agtgatcgct ggcgaaggta acgtaacaat cgcaggcgct gccggtaaga aagttgttat
     2641 cagcaacatc ttaggtcagg tagtagctaa cactgtcgtt tcttctgaca acgcaactat
     2701 cgctgctccg gcaggtgtag tagttgtagc tgttgaaggc gaagctgctg taaaagctat
     2761 cgttaagtaa cgattgatac taaaaaacaa tactgtctat cgtaagggtg gttagcgaac
     2821 cacccttaca caggcacaat gacattttat aatcaaataa ttctagacaa cctgcgtgaa
     2881 accgtatgta agaggttttc ttgatctacg gtttcaatta ccctgcgaca ggcgaacgag
     2941 cggttggatt taatattaat aatactaaag taatgaaatt aaaagttcta ctgcttttcc
     3001 ctctgtgaag accggacggc agacaaaaaa caggccccgg cggtattcgt actgccgggg
     3061 cttcgttttt atttccctta tccctatttg taggtatttt ctccggttta acatccctcg
     3121 tcctttacaa tccttttcag atagggggaa aagacgtatc tttgcgtgcc taaaccgaaa
     3181 tagacaagat aagcttatga agtttgaaaa cgagaactgg gcgcagttca aaggagaaac
     3241 ctggaaaaag gagatcaatg taagagactt tatccagaac aattacaaac cttataacgg
     3301 agacgatacg ttcctcgtac cctcttcgga gaaaacgaag aaagtgtgga acaaactaac
     3361 cgagatgttt aaggttgaaa gggagaaagg agtatatgat gccgaaacga agcttcccca
     3421 ggggattgac acatacggac ccggttacat cgataaggaa aacgaagtaa tcgtcggctt
     3481 gcagacagac gccccgttga agcgcggtat cttcccgaaa ggcggtatcc gcatggtcgg
     3541 aaacagtctg gaagcctatg gctatcatct ggacccgatg acaaaagaaa tcttcaccaa
     3601 atacagaaag acgcacaacg aaggcgtttt ttcagcctat acagacgaga tgattgcggc
     3661 acgtcgttcc gccatcatta ccgggttgcc tgatgcctac ggacgcggac gtatcatcgg
     3721 tgattaccgt cgtgtacctc tgtacggaac ccagatcctg atcgaagaaa agaaacgttt
     3781 ccagaaacgt ctcgatatac aggaactgac agaagaaatc attcagagtc gtgaggagat
     3841 aacggagcag atcaaagcgc tgaaagcatt tgagagaatg tgtgccggtt atggtttcga
     3901 cgtgacacgt ccggcaaagg atgcccgcga agccgtgcag ttcttgtatc tggcttacct
     3961 ggcagccgta aaggatcagg atggagcagc catgtctctc ggacgtactt ctactttcct
     4021 cgatatctat atcgaaaaag atatttgcga aggcaagctg acggaagaag aggcacagga
     4081 attgatcgac cagtttatta tcaaactgcg tattgtccgt ttcctgcgta ctccggaata
     4141 caacgacctg ttctccggcg accctgtttg ggtgactgaa tcgctgggcg gacaaggtgt
     4201 tgacggccgt tcgctggtga caaagacatc ttaccgttat ctgcatacct tgtataacct
     4261 cggcccggca cccgaaccca acctgacggt gctttggttc aacaatgctc cggataactg
     4321 gaaaaacttc tgcgcaaaag tatcgatcga tacctctgcc atccagtacg agaacgacga
     4381 tctgatgcgt ccggactatg gcgacgatta cggtatcgcc tgctgtgtgt ctccgatgaa
     4441 gatcggtaag cagatgcagt atttcggagc tcgtgccaac ctggcaaagt gcttgttgta
     4501 tgcgatcaat ggcggacgtg acgagaaatc gggtgtgcag gtggctcccc gtttcgaacc
     4561 gatcaccagc gagtatctgg attacaacga agtgatggag aaatacgagc agatgatgcg
     4621 ctggttggca aaagtttatg tcaatgctct gaagatcatt cactacatgc acgacaaata
     4681 tgcgtatgaa gcattcgaga tggggcttca tgacggagat gtggaacgta tccgcgcaac
     4741 cggtatcgcc ggcctttcta tcgttgccga ctcgctggct gccatccgtg acacgaaagt
     4801 gaaagtgatc cgtgacgaac gtggtttggc tgtcgacttc gaacgcgaaa gcgaatatgt
     4861 acctttcgga aacaatgacg accgcacgga cagtattgct gtcgacatta ccgagaagtt
     4921 catggaatat ctgcgccagc atcagacata ccgcaaggca actccgacac aatctatcct
     4981 gacgatcaca tcaaacgtgg tttacggaaa gaaaaccgga acgactcccg acggccgtcc
     5041 gggtggtacg ccgttcgctc cgggagccaa cccgatgaac ggacgcgaca cgaaaggggc
     5101 tgtcgccgct ttggcttctg ttgccaaact gcctttccag catgcgcatg acggtatctc
     5161 ctatacattt gccgtttcac cggctacatt gggtaaggag cgcgatattc agataaataa
     5221 tctggtgagc ctgttggatg gctatttcac gcctgacggc ggccagcatc tgaatgtgaa
     5281 cgtgttcgat aaggacctgt tggtcgatgc gatggagcac ccggagaaat acccgcagct
     5341 tacgatccgt gtttctggct atgccgtcaa cttcgtgaag ctgacacgcg aacagcagct
     5401 tgacgtcatc tcgcgcacca tcaacagcag tttataattg tccattgatt aatatgttgg
     5461 ggcgtataca ttctttagag agtttcggga ccgttgacgg tcccggaatt cgctttgtcg
     5521 tttttatgca gggatgtccg cttcgttgtc tgtattgcca caatccggat acctgggagg
     5581 taaagcgtga aacgccttat ctgctggaac ctgaagcgtt gcttgccgaa gtgctgcgct
     5641 ataagaattt tatcgctaaa ggtggtgtta ccgtgaccgg tggcgagcct ttgttacaac
     5701 ctgaattcct taaagagttt ttccgccttt gccgtgagaa cggtattcat acggcgttgg
     5761 acacttccgg ctatatctgt tccggtaaag cattggaggt gctcgaacag gtcgatctgg
     5821 ttctgttaga tatcaagacg atcgacgccg gtcttcatcc ccgtttgacg gccgtaaaac
     5881 tcgataatac tttacgcttt cttgatgaac tggagaaacg cggcatccct gtatggattc
     5941 gccacgtgat cgtgcccggc ctgacggacg atgatgaagc cttgagcaag ctggccgaat
     6001 atatcagttc atataacgtg gtccagaaag ccgaacttct gccttatcat acaatgggag
     6061 cttacaaata tgaggcgcaa ggcctggact ataagctgaa aggtgtggaa ccgctttcag
     6121 cagaacgtct ggccaatgcg aaagcgatct ttatgaaaca cggggtaacc gtataaacct
     6181 aatcgagtgt ctgctctttg atgatgatcg gtttacggct gatccggttg actgtgcgga
     6241 cgctgtcgct ttcaatactg gtaacatctc ccttgcagtt ggtgaaatag atgtaatgtc
     6301 ccatatccat gaaacgatag accttacacc cttcgtgttc gaacagatat tcgacttgat
     6361 aagtggaatt gtttaccggt ttttcctttg taaggggtat ttgtgagaca catgaagaaa
     6421 gacatgcgtt gcaaacgagc aatagacttg ctgaaaatgt attatacaaa ctagctcttc
     6481 tcatcttcga atatttttaa gttaacatcc aaagttaaca attaaagaat cacatggtat
     6541 gatcgaaagg tattttacct acaattaggt atatttttat tgttccggaa aacattactt
     6601 tgcttcctat tgtcatagta gataagtatg cttgcagata gaaatccgtc tatcggcaga
     6661 taaaaattat caatttgaaa aattctttca atcagtatct tacga
//
