GCA_037966755.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaGFPFHPBP_58 (original name was: gnl|Prokka|GFPFHPBP_58)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	terpene	44,973	65,854

gnlProkkaGFPFHPBP_67 (original name was: gnl|Prokka|GFPFHPBP_67)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS-like,NRPS	33,779	78,495

gnlProkkaGFPFHPBP_92 (original name was: gnl|Prokka|GFPFHPBP_92)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	cyclic-lactone-autoinducer	31,829	52,534

gnlProkkaGFPFHPBP_164 (original name was: gnl|Prokka|GFPFHPBP_164)

Region	Type	From	To	Most similar known cluster		Similarity
Region 22.1	RiPP-like	143,455	155,605

gnlProkkaGFPFHPBP_192 (original name was: gnl|Prokka|GFPFHPBP_192)

Region	Type	From	To	Most similar known cluster		Similarity
Region 33.1	T3PKS	145,648	186,817

Region	Type	From	To	Similarity
Region 7.1	terpene	44,973	65,854
Region 8.1	NRPS-like,NRPS	33,779	78,495
Region 12.1	cyclic-lactone-autoinducer	31,829	52,534
Region 22.1	RiPP-like	143,455	155,605
Region 33.1	T3PKS	145,648	186,817

Compact view

gnlProkkaGFPFHPBP_58 - Region 1 - terpene

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Location: 44,973 - 65,854 nt. (total: 20,882 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

additional biosynthetic genes

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaGFPFHPBP_67 - Region 1 - NRPS,NRPS-like

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Location: 33,779 - 78,495 nt. (total: 44,717 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 33778 - 78495:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala - D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 33778 - 76196:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala - D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 37592 - 78495:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala - D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg8_54

AMP-binding (11..374): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VVYNTAVCAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ctg8_57

Search NORINE for peptide: strict or relaxed

AMP-binding (8..381): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SVTQAAVAGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1000..1372): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSHGYGCVLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1967..2333): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLIVAAVIK
Nearest Stachelhaus code(s):: Ala DVWVVAAVIK (68% 8Å match); S-bohPhe or Phe DALIVAAVCK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2921..3307): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4327..4705): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DITQMGIVWK
Nearest Stachelhaus code(s):: Gly DILQMGMVWK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaGFPFHPBP_92 - Region 1 - cyclic-lactone-autoinducer

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Location: 31,829 - 52,534 nt. (total: 20,706 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaGFPFHPBP_164 - Region 1 - RiPP-like

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Location: 143,455 - 155,605 nt. (total: 12,151 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaGFPFHPBP_192 - Region 1 - T3PKS

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Location: 145,648 - 186,817 nt. (total: 41,170 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.