LOCUS       c00109_gnlPro..         6815 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LANDKDAI_109.
ACCESSION   c00109_gnlPro..
VERSION     c00109_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 21:56:54
            Original ID  :: gnl|Prokka|LANDKDAI_109
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6815
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6815
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2351:5617]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2352..5617
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6815
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6815
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(282..1229)
                     /locus_tag="ctg109_1"
                     /transl_table=11
                     /translation="MTTLIFGHQNPDTDAITSAMSWAEFQKQAGNTDVEAVALGEPNDE
                     TKFVLDHFKVQAPRVIKTAANETDHVMXXXXMLVDHNEFQQSVSDIEDVTIDSVVDHHR
                     IANFHTAAPLFYRAEPVGSTNTVIFEMFQEYGFKISPTLAGLMASGLISDTLLLKSPTA
                     TDKDKKILPEMAKIAGIDLQSYGLAMLKAGTNLAAKSDMDIIEGDAKSFTMGGKSIRIG
                     QVNTVDLNDIYARQDELINAMTKEAQAKGYDDFLLIATDILDSNSTGFAVGADRDKIAS
                     AFNEQFDKDARLALPGVVSRKKQVVPPMQSTFEG"
     CDS             complement(1259..2227)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     60.8; E-value: 3.2e-19)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg109_2"
                     /sec_met_domain="LysR_substrate (E-value: 7.5e-28,
                     bitscore: 85.7, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 2.6e-20, bitscore: 60.8,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKQERIFSSKTLTYFLQLAETMNYTQAAQLLGITQPALTQQIK
                     KLERTVGAPLFYSVGKKLHLSDAGQTMLKSTHEIYEILNQTTDEIQQTTSATVGKINIG
                     LLSSIEDSAFIAFIAHYYQLNPEVEVTLRMMTRHEIWESLENNQIDLAIMNLPDETIKN
                     WKPYASKKIIDEQLLFLHHDDALANRKRIHFKDTLAEPWATYPDDYFLGQLLREAYKNQ
                     MVDFPHVVAHFTTPYQLTQFVNLSGVNTALPTSFVRARADQIKAYAIAFDPAIRFEISF
                     VYRKEKADIPRISNFFQTFDDYLKEKDYLSRVSENRVKLDK"
     CDS             complement(2352..3203)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 65.2;
                     E-value: 2.4e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg109_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-26, bitscore:
                     82.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLIDQSQSPLNILEQVGQDHDGPIKGYVHSVESFGSVDGPGIRF
                     VVFMQGCRMRCQYCHNPDTWNIGVGEEMTADQILADAQRYKAFWGDQGGITCSGGESLV
                     QIDFILELFTKAKALGISTCLDTSGGPFTRDQPWFGQFEKLMAVTDISLVDIKHIDSAE
                     HKKLTGFPNENILDMVQYMSAHGDDMWIRHVLVPERTDFDPYLKRLGDYIATLDKNVVQ
                     KVEILPYHTLGVKKYHELGITYPLEGIEPPSADRVKNAENLLHVKDYTGWQSWRPKPVA
                     SN"
     CDS             complement(3335..5617)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg109_4"
                     /sec_met_domain="Gly_radical (E-value: 2e-42, bitscore:
                     132.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.2e-241, bitscore:
                     788.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLDETKVPNYWEGFNGGDWQEEINVRDFIEHNLNQYDGDESFL
                     AGPTEATTVLNNQVLNLKKQERANGGVLDADNNIPSTITSHGPGYLNKDLEKIVGVQTD
                     KPFKRAFMPFGGIRMAEDALESYGFKTDPQEHKIFNEYRKTHNQGVFDAYTPDMRKARH
                     YKIVTGLPDAYGRGRIVSDFPRIAVYGIDRLMAEKFKDYNLTGDGEMTDEVIKLREEIN
                     EQYRALNDMKKMAKEYGYDISRPAANAQEAVQWIYFGYLAAVKTQNGAAMSVGRIDTVI
                     DAYIQRDMRLGKLNEEQAQELIDHLVMKLRMVRFIRTEEYNSXXXXXXXXFSGDPIWAT
                     LSLAGMGNDGRHHVTKTAFRFLKTLDNMGAAPEPNITLLWSERLPEGFKRYATEVSIQS
                     STIQYENDDLMRNEWGTDYYGIACCVSAQPIADGVQYFGARANLAKTVLYAINGGKDEI
                     QEAQVGPEYAPITSDYIDYKEFMNKFDKMMDWLADTYVNALNVIHYMHDKYYYEAAQLA
                     LKDTQLNRTFATGISGLSHAVDSISAIKYGHVKAIRDENGVAVDFVADNDDYPRYGNND
                     DRADDIAKWLVKTFYNKMNTHHLYRGAKLSTSVLTITSNVVYGKNTGTTPNGRQKGEPF
                     SPGANPAYGAEKNGALASLMSTAKIPYHYATDGISNTFGVTPNTLGHDDETRKDTLVHM
                     VDGYMENSGMHLNINVFNKETLIDAQKHPEEYPTLTVRVSGYCVYFADLTKEQQDDVIA
                     RTFFEEM"
     CDS             complement(5888..6814)
                     /locus_tag="ctg109_5"
                     /transl_table=11
                     /translation="VSRDGYVKRSSLRSYQAVDPADNGLKANDLAIFTGELSTLQHLYI
                     VTNAGNVIYRPVHEISDARWKDTGEHLSQTVGLGTDEKVLAVFAFDQLDGAGSFVLGSS
                     DGYIKQTALSDLQPQRTYKSKPMMAMKLKDPAAIVTNAYFTTDQQQDVFVVSRHAYGLT
                     FPLSDVSTVGARATGVKSMDLKPDDEVVNFILVKDPTTAKVGILTQRGAFKKMALSEVG
                     EMSRARRGLLVLRELKRDPHRIVAMMSVDDATRLNVLTDTGKVTTLNPAQHPAGDRYSN
                     GSFVIDTDVLGKPVFVQTKEEPADQVE"
ORIGIN
        1 ttcgttaagt ccacaactat caagttgaat tcccgaactc gtctcagaat gcgtctctca
       61 ccatgcctca aaaccatcca aaatgaccca tgacaacttg ctgttgattc gacccgcgaa
      121 ttactacacg aatttcaaat tcacgcatca cgagttgcta gtcagcttta tatttaacag
      181 atttcgcaaa aagatggcta gagacttttt gcaaacaaaa aagccgcaac tggctagctg
      241 cgactttcct tagaacaatt gtgtgcaatt aaagatgaaa gttaaccttc aaacgtgctc
      301 tgcattggcg gaacgacctg ctttttgcgg gaaacaaccc caggcaatgc caaacgcgcg
      361 tccttgtcga actgttcgtt aaaagcactg gcgattttat cgcggtcagc accgactgcg
      421 aaaccagttg aattgctgtc caagatgtca gtagcaatca gcaggaaatc gtcgtagcct
      481 ttcgcctgcg cctctttggt catggcattg atcaattcgt cctgacgtgc gtaaatgtcg
      541 ttcagatcga cggtattgac ctgtccgatg cgaatagact tgccgcccat ggtgaaactc
      601 ttagcatcgc cttcgataat gtccatatct gacttggcgg ccaaattagt gcctgccttc
      661 agcatggcaa gaccatatga ctgcaaatca attccggcaa tcttcgccat ttctggcaaa
      721 atcttcttat ccttatcggt tgcggtcggc gacttcaaca acagcgtgtc tgaaatcaaa
      781 ccagaagcca tcaaaccagc caatgttggc gaaatcttga agccatattc ctggaacatc
      841 tcaaaaatga cggtattggt gctgccaacc ggttctgctc ggtaaaacag cggcgctgcc
      901 gtgtggaagt tagcgatgcg atgatggtcg acaacagaat caatcgtcac gtcttcgata
      961 tcactgacac tttgttgaaa ttcgttgtga tcaaccaaca tgnnnnnnnn nnacatgaca
     1021 tgatccgtct cgttggctgc cgtcttgata acccgcggtg cctgaacctt gaagtgatca
     1081 aggacgaact tagtttcatc gttcggttcg cctaatgcta ccgcctcaac atccgtgttg
     1141 cctgcctgtt tctgaaattc tgcccagctc atggcactcg taatcgcgtc ggtgtccggg
     1201 ttctgatgcc caaaaatcaa agtcgtcatc ttatctgatc gctcctcaac tgaataattt
     1261 acttatcaag tttaacacgg ttttcactga cccgcgacaa gtaatctttt tccttcagat
     1321 aatcgtcaaa ggtctggaag aagttcgaga tccgcggaat gtcagctttc tctttgcgat
     1381 aaacaaaaga aatctcaaag cgaatggccg gatcaaacgc aatagcatag gccttgattt
     1441 gatcagcgcg tgcccgaaca aaactggttg gtaaggccgt gttgacccct gacaagttaa
     1501 cgaattgggt taactgatat ggcgtggtga aatgtgcgac cacgtgcggg aaatcaacca
     1561 tttgattctt gtacgcctca cgcagaagct gtccaaggaa atagtcgtca ggataagttg
     1621 cccaaggttc agctaaagta tctttgaaat ggattcgctt acggttagcc aaggcgtcat
     1681 cgtggtgcaa gaacagcaat tgttcatcaa taattttctt gctggcgtat ggtttccagt
     1741 tcttaatggt ttcatctggc agattcatga tagccaaatc aatctggttg ttttccaatg
     1801 attcccaaat ttcgtgacgg gtcatcatcc gtagcgttac ttcaacttca ggattcagtt
     1861 gatagtaatg agcaatgaac gcaataaagg cgctatcctc gattgagctt aataacccaa
     1921 tattgatttt tccgacggtt gcactggttg tttgttgaat ttcgtcggtt gtctggttca
     1981 agatctcgta gatctcatgg gttgacttta acatggtctg accagcgtca gataaatgca
     2041 gtttcttccc gacagaataa aacagcggtg cccccactgt ccgttccaat ttcttgatct
     2101 gctgtgttaa ggctggctgg gtaatgccaa gcaattgggc ggcttgggtg tagttcatgg
     2161 tttctgccaa ttgcagaaaa taagttagtg tctttgagga aaaaatgcgc tcttgtttcg
     2221 ttttcatagg gcggcttccc tttcgtttat tatttaataa tatcgttcaa ccatcatcat
     2281 aagtggcaat gaaaaaaaac gcaacgaatt tgccgcgttt ttaatcgtta cgttaagttc
     2341 tagctaaaaa ttcagttgct cgcaactggt ttcggacgcc agctttgcca tccggtataa
     2401 tccttaacgt gcagcagatt ctctgcattc ttaacgcgat cggcagacgg cggttcgatg
     2461 ccttcaagcg ggtacgtaat gccaagctcg tgatactttt taacgcctaa cgtgtgatac
     2521 ggcagaatct caactttttg gaccacgttt ttgtccaatg tcgcaatata gtcccctaat
     2581 cgtttgagat aagggtcaaa gtcagtgcgt tccgggacca gaacgtggcg aatccacatg
     2641 tcatcaccat gcgccgacat atactgcacc atatctaaaa tattttcatt aggaaaaccg
     2701 gtgagctttt tatgctcggc cgaatcgatg tgtttaatat caactaatga gatgtcagta
     2761 acagccatca gcttttcaaa ctggccaaac cacggttggt cacgcgtgaa ggggccgcct
     2821 gacgtatcga ggcaagtcga aattcccagt gccttagcct tggtgaagag ttcgagaatg
     2881 aaatcgattt gtaccaaact ctcaccgcca ctgcacgtaa tacccccttg gtcaccccag
     2941 aatgctttat agcgctgggc atccgccaaa atttgatcgg ccgtcatttc ttcaccaacc
     3001 ccaatgttcc aggtgtcagg gttgtgacaa tactggcaac gcatgcgaca cccttgcata
     3061 aagacaacga agcgaatacc agggccatcg accgaaccaa aactttcgac cgagtgaaca
     3121 taccctttga tcgggccatc atgatcctga ccaacttgtt ctaagatatt taatgggctt
     3181 tgtgactgat cgatgagttt catcttattg cctccactac aacatcatgt gtcgttttca
     3241 attgattttc atttcattct gccaaaaaac ggcaacagca tcgttgccgt cttttggcaa
     3301 aacaggaatg gcttcctgtc ttgagttaag ctgcttacat ttcttcgaag aaggtccgag
     3361 caatgacatc gtcttgttgt tccttggtca aatctgcgaa gtagacgcag tagccagaaa
     3421 cccgaacggt caatgttggg tattcttctg ggtgcttctg agcatcaatc agggtctcct
     3481 tgttgaagac gttgatgttc aaatgcatgc cgctgttttc catatagccg tcaaccatgt
     3541 gaaccaaggt atccttacga gtttcatcat cgtggcccag tgtgttcgga gtaaccccaa
     3601 acgtattact aatgccgtca gttgcgtaat gatacggaat cttggcggtg gacatcaagg
     3661 atgccaatgc gccgttcttt tctgcgccgt atgccgggtt ggcacctggt gagaatggtt
     3721 cgcctttttg acgaccgttt ggtgttgtcc ctgtattctt accataaacc acgttggaag
     3781 taatggtcag gacactggta ctcaacttcg cgccgcgata cagatgatgc gtgttcatct
     3841 tgttatagaa ggtcttaacc aaccacttag cgatgtcatc agcgcgatca tcgttgttac
     3901 cataacgtgg atagtcatca ttgtcagcaa cgaagtcaac tgcaacaccg ttttcatcgc
     3961 gaattgcctt gacatgacca tacttgattg cactgatgga atcaaccgcg tgtgacaggc
     4021 cggaaatccc ggttgcgaag gtccggttca gttgggtatc cttcaatgcc agctgtgcag
     4081 cttcataata atacttgtca tgcatatagt gaatcacatt caaagcattg acatacgtgt
     4141 cggccagcca gtccatcatc ttgtcgaact tattcatgaa ttctttgtaa tcaatgtaat
     4201 cgctggtgat cggtgcatat tcaggcccta cttgcgcttc ctgaatttca tctttaccac
     4261 cgttgatcgc atacagaacc gtcttagcta agttagcacg ggcaccaaag tattgaacgc
     4321 catcagcaat tggttgtgcc gagacacagc aagcaatacc gtagtaatcg gtaccccatt
     4381 cattgcgcat caggtcatca ttttcatatt gaatggttga actttggatg gatacttcgg
     4441 ttgcatagcg tttaaagcct tctggcaagc gttcgctcca aagcaaggtg atgtttggtt
     4501 ctggtgctgc gcccatgttg tccaaagtct tcaggaaacg gaaagccgtc ttggtaacgt
     4561 ggtgacggcc atcattgccc attcctgcca atgacaaggt tgcccagatt ggatcgcctg
     4621 agaacnnnnn nnnnnnnnnn nnnnnnnnng agttatattc ttcagtccgg atgaaacgaa
     4681 ccatccgcaa tttcataacc aaatgatcaa tcagttcctg tgcctgttct tcgttcaatt
     4741 tgcctaagcg catatcgcgt tggatataag catcaataac cgtatcgatc cggccaacgg
     4801 acattgcggc accgttttga gtcttaacag cagccagata gccaaagtag atccactgaa
     4861 cagcttcttg cgcattagct gccggacgcg agatgtcata gccatattcc ttggccatct
     4921 tcttcatgtc gtttaaggca cgatattgtt cattgatttc ttcacgcaat ttgattactt
     4981 cgtcagtcat ttcgccgtcg ccggtcaagt tataatcctt gaacttctca gccattaaac
     5041 gatcgatccc gtaaacagcg atccgtggga aatcagaaac aatccggccg cggccgtatg
     5101 cgtctggcaa gccagtcacg atcttgtaat ggcgagcctt tcgcatatca ggggtataag
     5161 catcaaagac accttggtta tgggtcttac gatattcatt gaaaatcttg tgttcttgag
     5221 gatctgtctt gaagccataa gattccaatg catcttcagc cattcgaata ccgccaaatg
     5281 gcatgaatgc tcgtttgaat ggcttgtcgg tctgaacacc aacaatcttt tcaagatctt
     5341 tgttcaaata accagggccg tgtgaggtga tcgtagatgg aatattgttg tcagcatcca
     5401 aaacaccgcc atttgcacgt tcttgcttct ttaaatttag aacctgattg ttcaggactg
     5461 ttgtcgcttc agtggggcca gcaaggaaac tttcgtcccc gtcatattgg ttcaggttat
     5521 gctcgataaa atcgcgcaca ttaatttctt cttgccagtc gccgccgtta aagccttccc
     5581 aataattagg aactttggtt tcgtcaagct gtttcatagt tgtttgcctc ctaaagtgga
     5641 ttacagatgt gacagttctt acaaacacag tataacacnn nnnnnnnngt ataacacgcc
     5701 gccaaatcta tgcaagcgct tcattgccca tataataggc gttatattgt tttttaagtg
     5761 tgcttcgtcg cttcttttcg cccatgattt cggcattttg ttaaattgtg cacaactggt
     5821 ctgtcctata ataatcaaaa acgccaccat ctgtattagt acagacggcg gtgtcaatga
     5881 tcaccattta ttcgacttga tctgctggtt cttctttggt ttgaacaaaa actggcttgc
     5941 caaggacatc agtgtcaatg acaaacgaac cattcgaata gcggtcacct gctggatgct
     6001 gtgccggatt aagggtcgtt accttacccg tatcggtcaa aacattcagg cgggtagcgt
     6061 catcgactga catcatcgcg acaatccgat gcggatcacg cttaagctca cgcaagacca
     6121 gtaaaccacg acgggcacga ctcatttccc cgacttcgct taaggccatc tttttgaagg
     6181 cgccacgttg ggtcaaaata ccgaccttag ccgtagttgg atccttcact agaatgaaat
     6241 tgacaacctc atcgtctggc tttagatcca tacttttaac gccggttgcc cgggcaccga
     6301 cagttgacac atcgcttaac gggaatgtca gtccgtaagc gtggcggctg accacaaaga
     6361 catcctgttg ctgatcagtc gtgaagtatg cattggtgac aatcgcggct gggtctttca
     6421 gcttcatcgc catcatcggc ttactcttgt acgtccgttg cggctgcaaa tcacttaagg
     6481 cagtttgttt gatgtacccg tcacttgacc ctaacacaaa actgccagcg ccatcgagtt
     6541 gatcaaaagc aaacactgcc aagacttttt cgtcagtgcc aaggccaact gtttggctta
     6601 agtgttcgcc ggtgtctttc caacgcgcat cactaatttc atgcactggt cgataaatga
     6661 catttccggc atttgtcacg atgtaaagat gctgcaacgt actcagttcg ccagtgaaaa
     6721 tggcaagatc attggccttc aaaccattgt cggccggatc aaccgcctga taactgcgca
     6781 gtgaactgcg tttaacgtac ccatcacgag aaacc
//
