GCA_041320305.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaAALCGNDC_1 (original name was: gnl|Prokka|AALCGNDC_1)

Region	Type	From	To
Region 1	RiPP-like	524,224	534,631
Region 2	lassopeptide	588,609	610,882
Region 3	RiPP-like	1,200,089	1,211,648
Region 4	RiPP-like	1,685,799	1,696,191
Region 5	lanthipeptide-class-ii	2,254,044	2,277,202

gnlProkkaAALCGNDC_1 - Region 1 - RiPP-like

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Location: 524,224 - 534,631 nt. (total: 10,408 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaAALCGNDC_1 - Region 2 - lassopeptide

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Location: 588,609 - 610,882 nt. (total: 22,274 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lasso peptide predictions

Lassopeptide(s) for ctg1_544

ctg1_544

Leader:

MKDYVPPAVEVIASFKEATN

Full core:

GVWFGNYVDVGGAKAPFPWGSN

Putative macrolactam:

GVWFGNYVD

MIBiG 3.1 matches for ctg1_544

11.8% BGC0000535 (ADJ56352.1): Lanthipeptide: nisin A

12

GAKAPFPWGSN

22

G K G N

10

GCKTGALMGCN

20

antiSMASH-DB 4.0 matches for ctg1_544

100.0% NZ_CP062951.1and 1 other:

NZ_CP071248.1 Lassopeptides: BLI009_RS05010_lassopeptide

Lassopeptides: BL1347_RS03935_lassopeptide

1

GVWFGNYVDVGGAKAPFPWGSN

22

GVWFGNYVDVGGAKAPFPWGSN

1

GVWFGNYVDVGGAKAPFPWGSN

22

86.4% NZ_CP006714.1: Lassopeptides: B2258_RS10665_lassopeptide

1

GVWFGNYVDVGGAKAPFPWGSN

22

G+WFG YVD+GGAKAPFPWGSN

1

GLWFGKYVDIGGAKAPFPWGSN

22

45.5% NZ_NGKY01000001.1: Lassopeptides: A5819_RS19010_lassopeptide

1

GVWFGNYVDVGGAKA

15

GVWFG + DV G KA

1

GVWFGRWKDVFGGKA

15

with 8 more sequence matches

40.9% NZ_JAMDLY010000010.1and 4 others:

NZ_JAPDMW010000034.1 Lassopeptides: OF778_RS29475_lassopeptideNZ_JMLT01000016.1 Lassopeptides: BP95_RS33025_lassopeptideNZ_LS992241.1 Lassopeptides: PBLR_RS07315_lassopeptideNZ_SADY01000011.1 Lassopeptides: C7Y44_RS26425_lassopeptide

Lassopeptides: M5X04_RS09885_lassopeptide

1

GVWFGNYVDVGGAKA

15

G+WFG Y DV G +A

1

GIWFGRYRDVLGGRA

15

36.4% NZ_CP053549.1and 3 others:

NZ_JADKSM010000003.1 Lassopeptides: ITJ61_RS18070_lassopeptideNZ_PSXX01000017.1 Lassopeptides: C5E05_RS18625_lassopeptideNZ_PSXZ01000003.1 Lassopeptides: C5E07_RS02640_lassopeptide

Lassopeptides: HMI60_RS00570_lassopeptide

1

GVWFGNYVDVGGAKA

15

GVWFG + D+ G +A

1

GVWFGRWRDIFGGRA

15

36.4% NZ_KI912489.1: Lassopeptides: allorf_513617_513742_lassopeptide

1

GVWFGNYVDVGGAKAPF

17

G+W+G + D+ GAK F

1

GLWYGKFRDIFGAKTYF

17

36.4% NZ_KI912489.1: Lassopeptides: allorf_513868_514041_lassopeptide

1

GVWFGNYVDVGGAKA

15

GVWFG + D+ G +A

1

GVWFGKWTDIFGGRA

15

34.8% NZ_AULE01000001.1: Lassopeptides: H509_RS27430_lassopeptide

1

GVWFGNYVDVGGAKAPFP

18

G+W G Y DV G +A

1

GLWTGKYTDVFGGRALIK

18

34.8% NZ_JADPFA010000001.1: Lassopeptides: I4U27_RS00440_lassopeptide

1

GVWFGNYVDVGGAKA

15

G+W GN+ DV G +A

1

GLWTGNFRDVFGGRA

15

34.8% NZ_NCVL01000001.1and 1 other:

NZ_VMMF01000036.1 Lassopeptides: HT717_RS09510_lassopeptide

Lassopeptides: B7694_RS10650_lassopeptide

1

GVWFGNYVDVGGAKAPFP

18

G+WFG + D+ G +A F

1

GLWFGKFTDIFGGRAVFQ

18

27.3% NZ_LMUL01000017.1: Lassopeptides: allorf_410478_410597_lassopeptide

5

GNYVDVGGAKAPFPWG

20

G++V+ K P P G

4

GDFVETALTKQPIPMG

19

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lasso peptides

Core peptide predictions

ctg1_544 - Class II: Cleavage pHMM score: -3.90; RODEO score: 18; Monoisotopic mass: 2306.1 Da; Molecular weight: 2307.5 Da; Number of bridges: 0

gnlProkkaAALCGNDC_1 - Region 3 - RiPP-like

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Location: 1,200,089 - 1,211,648 nt. (total: 11,560 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaAALCGNDC_1 - Region 4 - RiPP-like

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Location: 1,685,799 - 1,696,191 nt. (total: 10,393 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaAALCGNDC_1 - Region 5 - lanthipeptide-class-ii

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Location: 2,254,044 - 2,277,202 nt. (total: 23,159 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg1_2055

ctg1_2058

Leader:

MSINEKSIVGESFEDLSAADMAMLTGRNDDGVAPA

Core:

SLSFAVSVLSVSFSACSVTVVTRLASCGNCK

Core with dehydrations:

DhaLDhaFAVDhaVLDhaVDhaFDhaACysDhaVDhbVVDhbRLADhaCysGNCysK

antiSMASH-DB 4.0 matches for ctg1_2058

100.0% NC_010816.1and 5 others:

NZ_CP069278.1 Lanthipeptides: JQN89_RS04800_lanthipeptideNZ_JADNCJ010000029.1 Lanthipeptides: I2B64_RS09750_lanthipeptideNZ_JADNLW010000041.1 Lanthipeptides: I2F45_RS10575_lanthipeptideNZ_JADPBO010000046.1 Lanthipeptides: I4V38_RS08850_lanthipeptideNZ_JADPES010000050.1 Lanthipeptides: I4U88_RS09655_lanthipeptide

Lanthipeptides: BLD_RS08345_lanthipeptide

1

SLSFAVSVLSVSFSACSVTVVTRLASCGNCK

31

SLSFAVSVLSVSFSACSVTVVTRLASCGNCK

1

SLSFAVSVLSVSFSACSVTVVTRLASCGNCK

31

96.8% NZ_CP046514.1and 12 others:

NZ_CP083257.1 Lanthipeptides: BCBL_RS08920_lanthipeptideNZ_CP084012.1 Lanthipeptides: LCQ81_RS01125_lanthipeptideNZ_CP084020.1 Lanthipeptides: LCQ52_RS09765_lanthipeptideNZ_GL622638.1 Lanthipeptides: HMPREF0177_RS02885_lanthipeptideNZ_JADMSA010000006.1 Lanthipeptides: I2H42_RS05020_lanthipeptideNZ_JADMUN010000007.1 Lanthipeptides: I1M16_RS07360_lanthipeptideNZ_JADNBL010000011.1 Lanthipeptides: I2A13_RS07655_lanthipeptideNZ_JADPBZ010000019.1 Lanthipeptides: I4V99_RS08285_lanthipeptideNZ_JADPCP010000015.1 Lanthipeptides: I4W34_RS09795_lanthipeptideNZ_JADPFY010000033.1 Lanthipeptides: I4V11_RS10065_lanthipeptideNZ_JAHTKJ010000004.1 Lanthipeptides: KUF58_RS04255_lanthipeptideNZ_SPGO01000021.1 Lanthipeptides: FGQ71_RS09210_lanthipeptide

Lanthipeptides: GN236_RS08545_lanthipeptide

1

SLSFAVSVLSVSFSACSVTVVTRLASCGNCK

31

SLSFAVSVLSVSFSACS TVVTRLASCGNCK

1

SLSFAVSVLSVSFSACSATVVTRLASCGNCK

31

32.3% NZ_CP036100.1and 1 other:

NZ_CP072062.1 Lanthipeptides: J5V93_RS29680_lanthipeptide

Lanthipeptides: EXW47_RS29590_lanthipeptide

7

SVLSVSFSACSVTVVTRLASCGNCK

31

S+L VS + C SC NCK

4

SILKVSRAVCKTGTCICTTSCSNCK

28

with 4 more sequence matches

32.3% NZ_KB976165.1and 1 other:

NZ_MOOT01000054.1 Lanthipeptides: BK765_RS17675_lanthipeptide

Lanthipeptides: ICC_RS37095_lanthipeptide

6

VSVLSVSFSACSVTVVTRLASCGNCK

31

S+L VS + C SC NCK

3

TSILKVSRAPCKSGTCLCTTSCSNCK

28

29.4% NZ_CP086145.1: Lanthipeptides: LL065_RS26230_lanthipeptide

3

SFAVSVLSVSFSACSVTV

20

SF VSV+S+ +A +VTV

12

SFLVSVISIELTASAVTV

29

22.6% NZ_CP051884.1and 2 others:

NZ_CP051884.1 Lanthipeptides: allorf_3265908_3266039_lanthipeptideNZ_CP051884.1 Lanthipeptides: allorf_3265908_3266039_lanthipeptide

Lanthipeptides: allorf_3265908_3266039_lanthipeptide

13

FSACSVTVVTRLASCGNCK

31

C V VTR A G+ +

1

HDRCQVDGVTRAAQTGDPR

19

14.9% NZ_CP110813.1: Lanthipeptides: allorf_3539006_3539200_lanthipeptide

22

TRLASCGNC

30

TRLA+ GNC

10

TRLAAEGNC

18

ctg1_2062

Leader:

MIRRYLPKRSEIRMDMAGE

Core:

LREIVDEADNRPMRVPDYRTPAEAFADELLELQDQQGCCTSK

Core with dehydrations:

LREIVDEADNRPMRVPDYRDhbPAEAFADELLELQDQQGCysCysDhbDhaK

allorf_2255785_2255907

Leader:

MYMAAVRVPYS

Core:

TSKYVARRSRKTKRLLYRMKRYRSLLVCD

Core with dehydrations:

DhbDhaKYVARRDhaRKDhbKRLLYRMKRYRDhaLLVCysD

MIBiG 3.1 matches for allorf_2255785_2255907

17.2% BGC0000553 (SSGG_RS09925): Lanthipeptide: SRO15-2212

24

SLLVCD

29

SLLVC+

6

SLLVCE

11

antiSMASH-DB 4.0 matches for allorf_2255785_2255907

100.0% NZ_JADNBL010000011.1and 2 others:

NZ_JADPCP010000015.1 Lanthipeptides: allorf_14967_15083_lanthipeptideNZ_NJNX01000015.1 Lanthipeptides: allorf_56513_56635_lanthipeptide

Lanthipeptides: allorf_64040_64156_lanthipeptide

1

TSKYVARRSRKTKRLLYRMKRYRSLLVCD

29

TSKYVARRSRKTKRLLYRMKRYRSLLVCD

1

TSKYVARRSRKTKRLLYRMKRYRSLLVCD

29

allorf_2273003_2273236

Leader:

MSWSGGSICLRLCCIAGDGGAGHHLSRFRNANRASGA

Core:

CLRAIMTLLPRIRIRRIVARIMVRTRASNRSYRRLAQHAR

Core with dehydrations:

CysLRAIMDhbLLPRIRIRRIVARIMVRDhbRADhaNRDhaYRRLAQHAR

antiSMASH-DB 4.0 matches for allorf_2273003_2273236

95.0% NZ_JADMUN010000007.1and 1 other:

NZ_JADNBL010000011.1 Lanthipeptides: allorf_79901_80134_lanthipeptide

Lanthipeptides: allorf_095586_095819_lanthipeptide

1

CLRAIMTLLPRIRIRRIVARIMVRTRASNRSYRRLAQHAR

40

CLRAIMTLLPRIRIRRIVARIMVR ASNRSYRRLAQHAR

1

CLRAIMTLLPRIRIRRIVARIMVRAMASNRSYRRLAQHAR

40

19.2% NZ_CP011129.1and 1 other:

NZ_CP013141.1 Lanthipeptides: allorf_5335460_5335672_lanthipeptide

Lanthipeptides: allorf_3828668_3828880_lanthipeptide

11

RIRIRRIVARIMVRTRASNRSYRRLAQHAR

40

IRRIV+ + N S ++HAR

6

EFTIRRIVSSFSQCEKEPNASSTSRSEHAR

35

17.5% NZ_FNJN01000003.1and 1 other:

NZ_FNJN01000003.1 Lanthipeptides: BLS25_RS16855_lanthipeptide

Lanthipeptides: BLS25_RS16855_lanthipeptide

10

PRIRIRRIVARIMVRTRASNRS

31

P IR+ RI+ + ++ T A +

3

PVIRLSRIICKTILITGACGST

24

allorf_2273261_2273392

Leader:

MRRRVPRLRRKPAA

Core:

NPISTYLLNAAPSSSPSPAKSRTTIYVTC

Core with dehydrations:

NPIDhaDhbYLLNAAPDhaDhaDhaPDhaPAKDhaRDhbDhbIYVDhbCys

antiSMASH-DB 4.0 matches for allorf_2273261_2273392

54.8% NZ_JADPBO010000046.1and 1 other:

NZ_JADPES010000050.1 Lanthipeptides: allorf_12542_12778_lanthipeptide

Lanthipeptides: allorf_12544_12780_lanthipeptide

1

NPISTYLLNAAPSSSPSPAKSRTTIYVTC

29

NPISTY N APSSSPSPAKSRT Y TC

14

NPISTYRPNVAPSSSPSPAKSRTMTYATC

42

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg1_2058 - Class II

Cleavage pHMM score: -6.20

RODEO score: 0

Monoisotopic mass: 2941.6 Da

Molecular weight: 2943.4 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2961.4 Da N = 1; 2979.5 Da N = 2; 2997.5 Da N = 3; 3015.5 Da N = 4; 3033.5 Da N = 5; 3051.5 Da N = 6; 3069.6 Da N = 7

ctg1_2062 - Class II

Cleavage pHMM score: -5.40

RODEO score: 0

Monoisotopic mass: 4767.3 Da

Molecular weight: 4770.2 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 4788.3 Da N = 1

allorf_2255785_2255907 - Class II

Cleavage pHMM score: -6.50

RODEO score: 0

Monoisotopic mass: 3556.1 Da

Molecular weight: 3558.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3576.3 Da N = 1; 3594.3 Da N = 2; 3612.3 Da N = 3; 3630.3 Da N = 4

allorf_2273003_2273236 - Class II

Cleavage pHMM score: -7.10

RODEO score: 0

Monoisotopic mass: 4770.8 Da

Molecular weight: 4773.8 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4791.8 Da N = 1; 4809.8 Da N = 2; 4827.9 Da N = 3

allorf_2273261_2273392 - Class II

Cleavage pHMM score: -6.70

RODEO score: 0

Monoisotopic mass: 2858.5 Da

Molecular weight: 2860.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2878.2 Da N = 1; 2896.2 Da N = 2; 2914.3 Da N = 3; 2932.3 Da N = 4; 2950.3 Da N = 5; 2968.3 Da N = 6; 2986.3 Da N = 7; 3004.4 Da N = 8; 3022.4 Da N = 9