LOCUS       c00144_gnlPro..         2724 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EJFHEANA_144.
ACCESSION   c00144_gnlPro..
VERSION     c00144_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2025-04-29 22:54:15
            Original ID  :: gnl|Prokka|EJFHEANA_144
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2724
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2724
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1236:2111]"
                     /cutoff="10000"
                     /detection_rule="(minimum(3, [AdoMet_dc, Amidinotransf,
                     Arginase, OKR_DC_1, OKR_DC_1_7_arginine, OTCace]) or
                     ((OKR_DC_1_5_ornithine or OKR_DC_1 or Orn_Arg_deC_N) and
                     minimum(1, [Arginase, OTCace, OTCace_N, PAD_porph])))"
                     /neighbourhood="10000"
                     /product="Arginine2putrescine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1237..2111
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(3, [AdoMet_dc, Amidinotransf,
                     Arginase, OKR_DC_1, OKR_DC_1_7_arginine, OTCace]) or
                     ((OKR_DC_1_5_ornithine or OKR_DC_1 or Orn_Arg_deC_N) and
                     minimum(1, [Arginase, OTCace, OTCace_N, PAD_porph])))"
                     /neighbourhood="10000"
                     /product="Arginine2putrescine"
                     /protocluster_number="1"
     cand_cluster    1..2724
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(3, [AdoMet_dc, Amidinotransf,
                     Arginase, OKR_DC_1, OKR_DC_1_7_arginine, OTCace]) or
                     ((OKR_DC_1_5_ornithine or OKR_DC_1 or Orn_Arg_deC_N) and
                     minimum(1, [Arginase, OTCace, OTCace_N, PAD_porph])))"
                     /kind="single"
                     /product="Arginine2putrescine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2724
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Arginine2putrescine"
                     /region_number="1"
                     /rules="(minimum(3, [AdoMet_dc, Amidinotransf, Arginase,
                     OKR_DC_1, OKR_DC_1_7_arginine, OTCace]) or
                     ((OKR_DC_1_5_ornithine or OKR_DC_1 or Orn_Arg_deC_N) and
                     minimum(1, [Arginase, OTCace, OTCace_N, PAD_porph])))"
                     /tool="gutsmash"
     CDS             3..200
                     /locus_tag="ctg144_1"
                     /transl_table=11
                     /translation="IYSTNMIESFNNVIKRKAKPKAEFPTEQSLDTFIGIQAMSYNERY
                     FNRIHKGFGQVQDTLESYFD"
     CDS             310..1164
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     71.2; E-value: 1.7e-22)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg144_2"
                     /sec_met_domain="LysR_substrate (E-value: 6.9e-32,
                     bitscore: 99.4, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 2e-23, bitscore: 71.2,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIKQLRYFLVAAEEKQITSAAKRLYIAQPPLSYQLKQLEIELGA
                     QLFKRTAHGIELTDAGQILQSYASEILSLSESVKNQVHKTVSGELGTIAIGMASSSTGL
                     IPMKSFQDLTKFYPEINFDIYEDNTFGIIDKLEKKTIDLGIVRTPYNRNGLNAKTLTTE
                     RMMAVTADPKFKDKTELRIKDLNEQPLIIYRRFEEIFNQTFAHHGIKPFYAVKCDDSRT
                     AITWAKRKMGIALVPESIASTYAPDDLIPIKHSNWITHLQLVWRKDQKVTPLMKRIIDS
                     LSR"
     CDS             1237..1422
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Arginine2putrescine: Amidinotransf"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg144_3"
                     /sec_met_domain="Amidinotransf (E-value: 5.6e-12, bitscore:
                     34.2, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDLIHVYSEIGKLETVMLHRPGRELENLSPDILNRMLIDDIPYL
                     KIAQKEHDYFAHTLEK"
     CDS             1572..1907
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Arginine2putrescine: Amidinotransf"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg144_4"
                     /sec_met_domain="Amidinotransf (E-value: 8.5e-27, bitscore:
                     82.9, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVNEMVKQIIAGIRKNEIQNATPSLADLAEDPDYPFYLDPMPNV
                     YFTRDQQAAIGNGMTINRMTFRARRRESLFMETVLKHHPDFKNANIPIWRDRYTHGRLE
                     GGDELIF"
     CDS             1914..2111
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Arginine2putrescine: Amidinotransf"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg144_5"
                     /sec_met_domain="Amidinotransf (E-value: 2e-19, bitscore:
                     58.7, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFLAIGISQRTSAAAITDLAHSLFGHSSYDTILAIKIPHNHAMMH
                     LDTVFTMINYDQCTVHPFID"
     CDS             2200..2334
                     /locus_tag="ctg144_6"
                     /transl_table=11
                     /translation="MFDIILKRKEGTNVLKGIKLRLYPNRTQQNQLEQMFGNDRFVWN"
     CDS             complement(2525..2722)
                     /locus_tag="ctg144_7"
                     /transl_table=11
                     /translation="IYSTNMIESFNNVIKRKAKPKAEFPTEQSLDTFIGIQAMSYNERY
                     FNRIHKGFGQVQDTLESYFD"
ORIGIN
        1 caatttactc caccaatatg atcgaatcct ttaacaacgt tatcaagcgt aaagctaagc
       61 ctaaagcaga gtttccaact gaacagtcgc ttgatacttt tattggcatt caggcgatga
      121 gctacaacga gcgctatttc aatcgaatcc acaaaggctt tggtcaggtt caggatacct
      181 tagaatccta ctttgattaa ataaaagatt aaaaaatcaa tctacgagaa aaatctattt
      241 acacaaaaaa tttgacagtt tcaaattttt aataagagat acaactaaaa taaaaaagat
      301 aaattattaa tgaatattaa acaattacgc tactttttag tagcagccga agaaaagcaa
      361 atcacttcag cagctaaaag actatacatt gcacaaccgc cgctgagtta tcaattaaag
      421 caactagaaa tagaattagg agcacaatta tttaagcgta ctgctcatgg aattgaatta
      481 acagatgctg gacaaatttt gcagagctat gctagtgaaa ttctctcttt atcagaaagc
      541 gtgaaaaatc aggttcataa aactgtttca ggtgaactag gcacgattgc aataggcatg
      601 gcttcatcat caactggact aattcctatg aaaagttttc aagatttaac taagttttat
      661 ccggaaatca actttgatat ttatgaagat aatacctttg gcattatcga taaattggaa
      721 aagaagacaa tcgatttagg aattgtgcgt acgccttata atcgtaatgg cttaaatgca
      781 aaaactttaa ctactgaaag aatgatggca gttactgctg atcccaaatt taaggataag
      841 acggaattaa ggattaaaga tctaaatgag cagcccctca ttatttatcg aagatttgaa
      901 gaaattttta accagacttt tgctcatcat gggattaagc cgttttatgc tgttaaatgt
      961 gatgattcta gaacagcgat tacttgggcg aagagaaaaa tggggattgc tcttgttcct
     1021 gaatctattg cttctacgta tgcacctgat gatcttattc ctattaaaca tagtaattgg
     1081 attacgcatt tgcaattagt gtggcgcaag gatcaaaagg tgacgccttt aatgaaaaga
     1141 ataatagatt cactaagccg ttaagattgt aacttctacc taattgatcc ataatcattg
     1201 taagcggata cataatttga attaggagac gctataatga ctgacttaat tcatgtttat
     1261 tccgaaattg gcaaattaga aacagttatg ctgcatcgac cgggaagaga attagaaaat
     1321 ctttcaccgg atatcttaaa tcgaatgtta attgacgata ttccatattt aaaaatagct
     1381 caaaaagagc atgactattt tgcccacact ttggaaaaat agggagttca tgtcgtttat
     1441 ttagaagatt tattagctca ctctttgact aatgaagatg tccgtacaaa atttgtagat
     1501 caattattag aagaatccaa tataaaaaag aagatccatt acatcaatta ctgatagact
     1561 atttaatagc aatgccagta aatgaaatgg ttaagcaaat aattgctggt attagaaaaa
     1621 atgaaataca aaatgcaact ccttcattag ccgatctagc tgaagatcca gattatccat
     1681 tttatttgga tccaatgcca aatgtgtatt ttactcgtga tcaacaagca gctattggta
     1741 atggcatgac tattaataga atgacattta gagctagaag acgtgagtca ttatttatgg
     1801 aaaccgtttt aaagcatcat cccgacttta aaaatgccaa tattcctatt tggcgtgatc
     1861 gttacactca tggacgttta gaaggtggcg atgagctgat tttttaaaca atcatgtttt
     1921 tagcaatcgg tatttctcaa agaacttccg ctgccgcaat cactgacctt gctcatagcc
     1981 tgtttggtca ttctagttat gacacaattt tagcaattaa aataccacat aatcatgcca
     2041 tgatgcatct ggatacagtc tttacaatga ttaattatga tcagtgtacc gttcatcctt
     2101 ttattgatta attgccgaag aaaacacggg attttaatcc cgtgatgaat ccggcctttt
     2161 ttagtttttt tgcttgttcc ccccttcttt cttaaacata tgtttgatat aatcttaaaa
     2221 agaaaggagg gaacaaatgt gctaaaaggg atcaaattaa gactctatcc taatagaaca
     2281 caacaaaacc agcttgagca gatgtttggc aatgatcgtt ttgtttggaa ttaaatgctg
     2341 gccatgatga atgagcgcta tcagaataat aaagctttgc cttttttagg caagttcaag
     2401 ctgaattatc tgcttaagcc gcttaaaaaa gaatatcttt tttgaaaaac agcgattctt
     2461 cagaaactgt caaatttttt gtgtaaatag atttttctcg tagattgatt ttttaatctt
     2521 ttatttaatc aaagtaggat tctaaggtat cctgaacctg accaaagcct ttgtggattc
     2581 gattgaaata gcgctcgttg tagctcatcg cctgaatgcc aataaaagta tcaagcgact
     2641 gttcagttgg aaactctgct ttaggcttag ctttacgctt gataacgttg ttaaaggatt
     2701 cgatcatatt ggtggagtaa attg
//
