GCA_047463375.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaJAKIAOLI_1 (original name was: gnl|Prokka|JAKIAOLI_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	NRPS,T1PKS	56,394	112,020
Region 1.2	T3PKS	141,124	182,290

gnlProkkaJAKIAOLI_3 (original name was: gnl|Prokka|JAKIAOLI_3)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	RiPP-like	105,796	128,583
Region 3.2	RiPP-like	199,770	210,066

gnlProkkaJAKIAOLI_5 (original name was: gnl|Prokka|JAKIAOLI_5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	RiPP-like	115,693	127,789

Region	Type	From	To	Similarity
Region 1.1	NRPS,T1PKS	56,394	112,020
Region 1.2	T3PKS	141,124	182,290
Region 3.1	RiPP-like	105,796	128,583
Region 3.2	RiPP-like	199,770	210,066
Region 5.1	RiPP-like	115,693	127,789

Compact view

gnlProkkaJAKIAOLI_1 - Region 1 - NRPS,T1PKS

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Location: 56,394 - 112,020 nt. (total: 55,627 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 56393 - 112020:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - X - X) + (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_69

PKS_AT (609..880): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 62.5%; Ethylmalonyl-CoA: 58.3%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 84.8
Methylmalonyl-CoA: 75.4
Malonyl-CoA: 71.6
Isobutyryl-CoA: 44.2
Ethylmalonyl-CoA: 35.2
Propionyl-CoA: 24.7
Benzoyl-CoA: 24.7
inactive: 22.7
Acetyl-CoA: 20.2
fatty_acid: 20.1
CHC-CoA: 17.4
2-Methylbutyryl-CoA: 15.5
3-Methylbutyryl-CoA: 14.4
trans-1,2-CPDA: 12.2

AMP-binding (1518..1897): X

nrpys: Ph-Ser, Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFTVAAIWK
Nearest Stachelhaus code(s):: Ph-Ser or Phe DAFTVAAIWK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2553..2926): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHFGYQGK
Nearest Stachelhaus code(s):: Glu or D-Glu DPRHSGVVGK (56% 8Å match); D-Glu DPRHSGVVGK (53% 8Å match); Htia DPHHFGLIGK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

ctg1_71

Search NORINE for peptide: strict or relaxed

AMP-binding (445..841): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code(s):: Ser DVWHISLIDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_77

Search NORINE for peptide: strict or relaxed

AMP-binding (30..443): Hpr

nrpys: Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: pip (Hpr)

Stachelhaus prediction details:

Stachelhaus sequence:: DMGILGALVK
Nearest Stachelhaus code(s):: Pca DMGVLGCLMK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

gnlProkkaJAKIAOLI_1 - Region 2 - T3PKS

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Location: 141,124 - 182,290 nt. (total: 41,167 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS substrates

NRPS/PKS substrate predictions

gnlProkkaJAKIAOLI_3 - Region 1 - RiPP-like

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Location: 105,796 - 128,583 nt. (total: 22,788 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaJAKIAOLI_3 - Region 2 - RiPP-like

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Location: 199,770 - 210,066 nt. (total: 10,297 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaJAKIAOLI_5 - Region 1 - RiPP-like

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Location: 115,693 - 127,789 nt. (total: 12,097 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.