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Overview
2.1

### Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaKEKGBJKA_2 (original name was: gnl|Prokka|KEKGBJKA_2)

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 2.1 | thiopeptide | 1 | 22,222 | lactocillin | RiPP:Thiopeptide | 100%

Region | Type | From | To | Most similar known cluster | Similarity
--- | --- | --- | --- | --- | ---
Region 2.1 | thiopeptide | 1 | 22,222 | lactocillin | RiPP:Thiopeptide | 100%

No secondary metabolite regions were found in these records:
  gnlProkkaKEKGBJKA_1 (original name was: gnl|Prokka|KEKGBJKA_1)
  gnlProkkaKEKGBJKA_3 (original name was: gnl|Prokka|KEKGBJKA_3)
  gnlProkkaKEKGBJKA_4 (original name was: gnl|Prokka|KEKGBJKA_4)

Compact view
gnlProkkaKEKGBJKA_2 - Region 1 - thiopeptide
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
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Location: 1 - 22,222 nt. (total: 22,222 nt) Show pHMM detection rules used
Region on contig edge.
thiopeptide: ((YcaO or TIGR03604) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
KnownClusterBlast
Thiopeptides
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
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Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
Similar known gene clusters from MIBiG 3.1
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Thiopeptide predictions
Lists the possible core peptides for each biosynthetic enzyme. Predicted tail sequences are also shown, if present.
Includes CompaRiPPson results for any available databases, with a detailed explanation here.

#### Thiopeptide(s)

ctg2_8
Leader:
MKDLFDIDEKSLENLDLGDFKILDSEDLSEKDEKSILGA
Core:
S C T T C T C C C S C C
Core with dehydrations:
Dha Cys Dhb Dhb Cys Dhb Cys Cys Cys Dha Cys Cys
ctg2_8
Cleaved off residues:
A

#### MIBiG 3.1 matches for ctg2_8

31.2% BGC0001155 (AGY49587.1): Thiopeptide: GE2270
4
T C T C C C S C
11
C C C S C
5
V C G F C C S C
12
14.7% BGC0000535 (ADJ56352.1): Lanthipeptide: nisin A
4
T C T C C C S
10
T T C C S
23
T A T C H C S
29

#### antiSMASH-DB 4.0 matches for ctg2_8

100.0% NZ_CP040501.1 and 2 others:
NZ_CP054397.1 Thiopeptides: HR117_RS09200_thiopeptide NZ_LR595858.1 Thiopeptides: FQT45_RS06980_thiopeptide
Thiopeptides: FFA26_RS09695_thiopeptide
1
S C T T C T C C C S C C
12
S C T T C T C C C S C C
1
S C T T C T C C C S C C
12
69.2% NZ_QQBC01000004.1: Thiopeptides: DFR76_RS13815_thiopeptide
1
S C T T C T C C C S C C
12
S C T+C C C S C C
1
S C T S C V C I C S C C
12

#### Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

#### Types:

Type I: Featuring genes encoding indolic acid side ring (series e)
Type II: Featuring the gene coding for quinaldic acid moiety formation (series a, b, and c)
Type III: No genes for synthesizing L-Trp derivatives (series d)
Thiopeptides
Core peptide predictions
Lists the possible core peptides in the region. Each core peptide lists its possible molecular weights and the scores for cleavage site prediction and RODEO. If relevant, other features, such as macrocycle and amidation, will also be listed.

ctg2_8
  Putative Type III
  Cleavage pHMM score: 7.10
  RODEO score: 26
  Monoisotopic mass: 1216.3 Da
  Molecular weight: 1217.5 Da
  Predicted peptide features:
  Central ring: pyridine trisubstituted
  Macrocycle: 26-member
  Alternative weights
    (assuming N unmodified Ser/Thr residues)
    1235.5 Da N = 1
    1253.5 Da N = 2
    1271.5 Da N = 3
    1289.6 Da N = 4
    1307.6 Da N = 5