LOCUS       gnlProkkaBGDDINPK_448   3916 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BGDDINPK_448.
ACCESSION   gnlProkkaBGDDINPK_448
VERSION     gnlProkkaBGDDINPK_448
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 23:33:41
            Original ID  :: gnl|Prokka|BGDDINPK_448
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3916
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..630
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     195.4; E-value: 2.1e-59)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg448_1"
                     /sec_met_domain="AMP-binding (E-value: 5.6e-46, bitscore:
                     145.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEASPVITQTRANDSFIRRVETTGRALPHTEVKIVEPGTCQEVQR
                     GMQGELCTRGYHVMKGYYKDKEATRKAINHDGWLFTGDLAVMDEDGYCRITGRLKDMLI
                     RGGENIYPREIEEFLYRHPAVLDVQVVGVPDAKFGEEAAAWIKLKDGKSVSPDELKAYC
                     KGKIARHKIPRYVIFTDDYPMTASGKIQKYKLREKTIEMFNLSSSQ"
     protocluster    1..3916
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[0:3124]"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3124
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     cand_cluster    1..3916
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..3916
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="betalactone"
                     /region_number="1"
                     /rules="(HMGL-like and AMP-binding)"
                     /tool="antismash"
     CDS             640..1974
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090:acetyl-CoA carboxylase biotin carboxylase (Score:
                     651.2; E-value: 3.5e-197)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg448_2"
                     /sec_met_domain="ATP-grasp (E-value: 3.9e-11, bitscore:
                     31.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 4.2e-18,
                     bitscore: 54.0, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFTKVLIANRGEIAMRIIRTCSRLGIKTVAVYSEADKDALHTKAA
                     TEAYLIGESRVSESYLNIERIIKTAKKANADAIHPGYGLLSENSRFAERCKQENIVFIG
                     PSPDIIAKMGSKIEARKAMEAAGVPVVPGVSESLGDIEAACRTACQIGYPVMLKASAGG
                     GGIGMQRVENEEALKKAYEGNKKRAADFFGDGSMYIEKVIEHARHIEVQLLADQHGHTV
                     HLFERDCSVQRRHQKVIEEAPSPFVDDELRMKIGQTAVKAAKAIGYTNAGTIEFIVDQK
                     QNFYFLEMNTRLQVEHPVTEEITGLDLVEQQLRIAAGHTLTFSQKDIQRNGHAIEVRIY
                     AEDPKTFFPSPGTITAFSLPDQKGVRHECAVAKDSTVTPFYDPMIAKMIVKGQTRTEAI
                     EKLETSLRDYRVEGIKTNLPLLIQAAATKAFKEGDVTTDFLKQHL"
     CDS             1989..2210
                     /locus_tag="ctg448_3"
                     /transl_table=11
                     /translation="MTVSIQMAGNLWKVHVKAGDQIEKGQEVAILESMKMEIPIVADRS
                     GIVKEVKKNEGDFVNEGDVLLELSNSTQ"
     CDS             2225..3124
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271:2-isopropylmalate synthase (Score: 265.7;
                     E-value: 1.4e-80)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg448_4"
                     /sec_met_domain="HMGL-like (E-value: 5.5e-52, bitscore:
                     165.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYPKKVKIKEVGPRDGLQNEPVWIATEDKITWINQLSRTGLSYI
                     EITSFVHPKWIPALRDAIDVAKGIDRLKGVTYAALVPNQRGLENALEGGINEACVFMSA
                     SETHNRKNINKSTSESLHILKQVNNDAQKANLTTRAYLSTVFGCPYEKDVPIEQVIRLS
                     EALFEFGISELSLGDTIGAANPAQVETVLEALLARFPANQIALHFHDTRGTALANMVTA
                     LQMGITVFDGSAGGLGGCPYAPGSSGNAATEDIVYMLEQMDIKTNVKLEKLLSAAKWIE
                     EKMGKPLPSRNLQVFKSS"
     CDS             3145..3915
                     /NRPS_PKS="Domain: ECH (10-252). E-value: 2.1e-70. Score:
                     228.7. Matches aSDomain: nrpspksdomains_ctg448_5_ECH.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023:enoyl-CoA hydratase (Score: 262.8; E-value:
                     4e-80)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg448_5"
                     /transl_table=11
                     /translation="MGDSILFTVKNEHMALITFNRPQAANALSAEMLRNLQMILQEIEF
                     NSNIRCVILTGTGEKAFCAGADLKERIKLKEDQVLESVSLIQRTAALLDALPQPVIAAI
                     NGSALGGGLELALACDLRIAAESAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKEFIY
                     TGRRVTAHEAKEIGLVEHVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDL
                     ATGLAIEQKAYEQTIPTKDRREGLQAFQEKRRAVY"
     aSDomain        3175..3900
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg448_5_ECH.1"
                     /evalue="2.10E-70"
                     /label="ctg448_5_ECH.1"
                     /locus_tag="ctg448_5"
                     /protein_end="252"
                     /protein_start="10"
                     /score="228.7"
                     /tool="antismash"
                     /translation="NEHMALITFNRPQAANALSAEMLRNLQMILQEIEFNSNIRCVILT
                     GTGEKAFCAGADLKERIKLKEDQVLESVSLIQRTAALLDALPQPVIAAINGSALGGGLE
                     LALACDLRIAAESAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKEFIYTGRRVTAHEA
                     KEIGLVEHVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDLATGLAIEQKA
                     YEQTIPTKDRREGLQAFQEK"
ORIGIN
        1 accgaagcct cgccagtcat tacgcaaacg agagcaaatg attccttcat aagaagagtc
       61 gaaacaaccg gccgcgccct gccacatact gaggtgaaaa ttgtagaacc tgggacatgt
      121 caagaagttc aaagaggcat gcagggagag ctgtgcaccc gtggctatca cgtcatgaaa
      181 ggctattata aagacaaaga agcgaccaga aaagcaatca atcatgacgg atggctgttt
      241 accggagatc ttgctgtcat ggatgaagac ggatactgcc gcatcaccgg aagattaaaa
      301 gatatgctca tcagaggcgg cgagaacatt tatccgcggg aaattgaaga atttttatac
      361 cggcatcccg ctgttttaga tgtacaggtc gttggtgtgc ctgacgccaa attcggggag
      421 gaagctgcag cctggattaa actgaaagac ggtaaaagcg tttcacctga tgagcttaaa
      481 gcctattgca aagggaaaat cgcccgccac aaaattccgc gttatgttat ttttacggat
      541 gactatccga tgacggcctc aggcaaaatt caaaaatata aactgcgaga aaaaacgatt
      601 gaaatgttca acctatcatc aagtcaatga ggtgaaatca tgtttacaaa agtactgatc
      661 gccaaccgcg gagaaattgc aatgagaatt atccgaacat gcagccgtct cggcattaaa
      721 acggttgcgg tttattcaga agccgacaag gacgcgctcc atacaaaagc cgctacagag
      781 gcttatttga tcggagaatc gagagtcagt gaaagttatt taaatataga gagaatcata
      841 aagacggcga aaaaagcaaa tgccgacgcg atccacccgg gatatggatt gttatcagaa
      901 aacagtcggt tcgctgaacg ctgcaagcaa gaaaacatcg tgtttatcgg accttcccct
      961 gatatcatcg caaagatggg cagcaaaatt gaagcgcgaa aagcaatgga ggctgcaggt
     1021 gtccctgtgg taccgggcgt ttctgaatcc ctcggagata tagaggcagc ctgccgcacc
     1081 gcatgtcaaa tcggctatcc tgtcatgctg aaagcttcag cgggcggagg gggcatcgga
     1141 atgcagcgtg ttgaaaatga agaagcatta aaaaaagcgt acgagggaaa caaaaagcgc
     1201 gcagcagatt ttttcggtga cgggtcgatg tatatagaaa aagttattga acatgcgcgt
     1261 cacatcgagg ttcagctttt ggctgatcaa cacggccata cagtacatct gtttgaacgt
     1321 gattgctctg ttcagaggcg ccaccaaaaa gtcattgaag aagcaccgtc tccatttgta
     1381 gacgatgaac taagaatgaa gattgggcaa acagcggtaa aagcagcaaa ggcaatcggc
     1441 tatacgaacg caggcaccat cgaattcata gttgaccaga agcaaaattt ttatttcctc
     1501 gaaatgaata cgagactgca agttgaacac cccgtgactg aagaaataac aggcctggac
     1561 ttagttgagc agcagctgcg gattgctgcg ggccatacac tcacattctc ccaaaaagac
     1621 atccaacgga acgggcatgc gatagaggtt cgaatatacg cggaagatcc caagaccttc
     1681 ttcccgtcac caggtacgat cactgcgttt tcacttcctg accaaaaagg agtcagacac
     1741 gaatgtgcgg tagcaaaaga cagcaccgtt accccttttt atgacccgat gatcgctaag
     1801 atgattgtca aaggccaaac cagaacagaa gcaattgaaa aactagagac atcgcttcgc
     1861 gactatcgtg tagagggaat caaaacaaat cttccgcttc tcatacaggc tgcggcaaca
     1921 aaggcattta aagaagggga tgtcacgact gactttttga aacaacacct ataaaggagg
     1981 cagttgtcat gacggttagc atacaaatgg caggaaacct gtggaaagtc cacgtcaaag
     2041 ccggcgacca aatcgaaaag ggccaggagg tagccatttt ggaatcgatg aaaatggaaa
     2101 tcccgatcgt tgcagataga agcggaatcg taaaagaagt caaaaaaaat gaaggagact
     2161 ttgtaaatga aggagacgta ctgctcgaac tgagcaattc cactcaataa tttggaggaa
     2221 gaccatgtca tatcctaaaa aagtgaaaat caaagaagtc ggcccgcgtg acggcttaca
     2281 aaacgagccc gtatggatcg caacagagga taaaataacc tggatcaacc agctttcccg
     2341 gacagggctt tcgtatattg aaatcacatc cttcgttcac ccgaaatgga ttccggcgct
     2401 tcgagatgct atcgacgtag caaaaggcat cgaccgttta aaaggggtaa cgtacgcggc
     2461 acttgtcccg aatcaaagag gattggagaa tgcgcttgaa ggaggcatta acgaggcttg
     2521 cgtttttatg tccgccagcg agacgcacaa cagaaaaaac atcaataaat ccacttctga
     2581 atcccttcat atactcaaac aagtaaacaa cgacgcacaa aaagcaaacc tcacaacaag
     2641 agcctacctc tcgactgttt tcggctgtcc gtacgaaaaa gatgtcccca ttgaacaagt
     2701 cattcgcctt tcagaagctc tatttgaatt tgggatttct gaactttcgc ttggagatac
     2761 gattggagcg gctaatcccg cccaagtgga aactgtactt gaagctcttt tggcacgatt
     2821 cccggctaat caaattgcct tgcattttca tgatacgaga ggaaccgctc tggccaacat
     2881 ggtcacagca ctccaaatgg gcattacggt gtttgacggc tcggcaggcg ggcttggggg
     2941 atgcccatat gcgccaggtt catcaggaaa cgccgcaact gaggatatcg tgtacatgct
     3001 tgaacagatg gatatcaaaa caaatgtaaa gctagaaaaa ctgctatctg cggccaaatg
     3061 gatagaagaa aaaatgggca aaccgctgcc gagcagaaat ttacaggtgt ttaaatcatc
     3121 ttgataaaag ggaggacgca tacaatggga gactctattc tttttactgt taaaaatgaa
     3181 catatggcgt tgatcacctt taacaggcct caggcagcaa atgctctttc agcggaaatg
     3241 cttagaaacc tgcaaatgat tcttcaggaa attgaattca actcaaacat ccgctgcgtc
     3301 atcctcacag gcaccggtga aaaagcgttt tgtgcagggg cagacctaaa ggaacggata
     3361 aaactgaaag aagatcaggt tctggaaagt gtatctctca ttcaaagaac ggcggcttta
     3421 cttgatgcct tgccgcagcc ggtcatagct gcgataaatg gaagcgcatt aggcggcgga
     3481 ctagaattgg cattggcatg cgaccttcga atcgcagctg aatcagctgt gctgggactt
     3541 ccggaaacag ggttagctat tatcccgggc gctggaggga cccaaaggct gccccggctg
     3601 attggcagag gaaaagcaaa agaattcatt tatacaggca gacgcgtgac cgcacacgaa
     3661 gcaaaagaaa tcggccttgt agagcatgtc acggctcctt gtgaccttat gccaaaagca
     3721 gaggaactgg ccgcagccat ttctgccaac gggccgatcg ctgtccgtca ggctaaattt
     3781 gcaatcaata aaggattgga gacagatctc gctacaggcc ttgcgattga acaaaaagcg
     3841 tatgaacaaa ccatcccgac aaaagacagg agagaagggc ttcaggcctt tcaagaaaaa
     3901 agacgggccg tataca
//
