LOCUS       gnlProkkaBGDDINPK_70    4335 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BGDDINPK_70.
ACCESSION   gnlProkkaBGDDINPK_70
VERSION     gnlProkkaBGDDINPK_70
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 23:33:41
            Original ID  :: gnl|Prokka|BGDDINPK_70
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4335
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4335
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2:2312]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..2312
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..4335
                     /SMILES="NC(C(O)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..4335
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             3..2312
                     /NRPS_PKS="Domain: AMP-binding (12-326). E-value: 6.4e-80.
                     Score: 260.5. Matches aSDomain:
                     nrpspksdomains_ctg70_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (432-499). E-value: 8.3e-29. Score:
                     91.5. Matches aSDomain: nrpspksdomains_ctg70_1_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (519-749). E-value:
                     2.1e-38. Score: 124.9. Matches aSDomain:
                     nrpspksdomains_ctg70_1_Thioesterase.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     313.9; E-value: 2.6e-95)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg70_1"
                     /sec_met_domain="AMP-binding (E-value: 4.7e-98, bitscore:
                     316.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.4e-18, bitscore:
                     53.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 9.3e-11, bitscore:
                     30.8, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQPAFIMTNTKAANHIPPVENVPKIVLDDPELAEKLNTYPAENPK
                     NKDRTQPLSPLNTAYVIYTSGSTGVPKGVMIPHQNVTRLFAATEHWFRFSSDDIWTMFH
                     SYAFDFSVWEIWGPLLHGGRLVIVPHHVSRSPEAFLRLLVKEGVTVLNQTPSAFYQFMQ
                     AEREQPDLGQALSLRYVIFGGEALELSRLEDWYNRHPENRPQLINMYGITETTVHVSYI
                     ELDRSMAALRANSLIGCGIPDLGVYVLDERLQPVPPGVAGELYVSGAGLARGYLGRPGL
                     TSERFIADPFGPPGTRMYRTGDVARLRADGSLDYVGRADHQVKIRGFRIELGEIKAALV
                     QHPQLEDAAVIVREDQPGDKRLAAYVIPSEETFDTAELRKYAAERLPDYMVPAAFMTMK
                     ELPLTPNGKLDRKALPAPDFAAAVTGRGPRTPQEEILCDLFMEVLHLPRVGIDDRFFDL
                     GGHSLLAVQLMSRIREALGVELSIGNLFEAPTVAGLAERLEMGSSQSALNVLLPLRTSG
                     DKPPLFCVHPAGGLSWCYAGLMTNIGTDYPIYGLQARGIGLREELPKTLDDMAADYIKQ
                     IRTVQPKGPYHLLGWSLGGNVVQAMATQLQNQGEEVSLLVMLDAYPNHFLPIKEAPDDE
                     EALIALLALGGYDPDSLGDKPLDFEAAIEILRSDGSALASLDETVILNLKNTYVNSVGI
                     LGSYKPKTFRGNVLFFRSTIIPEWFDPIEPDSWKPYINGQIEQIDIDCRHKDLCQPEPL
                     AQIGKVLAVKLEELNK"
     aSDomain        39..980
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg70_1_AMP-binding.1"
                     /evalue="6.40E-80"
                     /label="ctg70_1_AMP-binding.1"
                     /locus_tag="ctg70_1"
                     /protein_end="326"
                     /protein_start="12"
                     /score="260.5"
                     /specificity="substrate consensus: Thr"
                     /tool="antismash"
                     /translation="ANHIPPVENVPKIVLDDPELAEKLNTYPAENPKNKDRTQPLSPLN
                     TAYVIYTSGSTGVPKGVMIPHQNVTRLFAATEHWFRFSSDDIWTMFHSYAFDFSVWEIW
                     GPLLHGGRLVIVPHHVSRSPEAFLRLLVKEGVTVLNQTPSAFYQFMQAEREQPDLGQAL
                     SLRYVIFGGEALELSRLEDWYNRHPENRPQLINMYGITETTVHVSYIELDRSMAALRAN
                     SLIGCGIPDLGVYVLDERLQPVPPGVAGELYVSGAGLARGYLGRPGLTSERFIADPFGP
                     PGTRMYRTGDVARLRADGSLDYVGRADHQVKIR"
     aSModule        39..2249
                     /complete
                     /domains="nrpspksdomains_ctg70_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg70_1_PCP.1"
                     /domains="nrpspksdomains_ctg70_1_Thioesterase.1"
                     /final_module
                     /locus_tags="ctg70_1"
                     /monomer_pairings="Thr -> Thr"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       177..236
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg70_1_0001"
                     /evalue="3.70E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg70_1"
                     /protein_end="78"
                     /protein_start="58"
                     /score="34.7"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGVPKGVMIPH"
     CDS_motif       771..860
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg70_1_0002"
                     /evalue="3.90E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg70_1"
                     /protein_end="286"
                     /protein_start="256"
                     /score="54.3"
                     /tool="antismash"
                     /translation="PPGVAGELYVSGAGLARGYLGRPGLTSERF"
     CDS_motif       945..1007
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg70_1_0003"
                     /evalue="2.30E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg70_1"
                     /protein_end="335"
                     /protein_start="314"
                     /score="38.7"
                     /tool="antismash"
                     /translation="YVGRADHQVKIRGFRIELGEI"
     aSDomain        1299..1499
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg70_1_PCP.1"
                     /evalue="8.30E-29"
                     /label="ctg70_1_PCP.1"
                     /locus_tag="ctg70_1"
                     /protein_end="499"
                     /protein_start="432"
                     /score="91.5"
                     /tool="antismash"
                     /translation="EEILCDLFMEVLHLPRVGIDDRFFDLGGHSLLAVQLMSRIREALG
                     VELSIGNLFEAPTVAGLAERLE"
     aSDomain        1560..2249
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg70_1_Thioesterase.1"
                     /evalue="2.10E-38"
                     /label="ctg70_1_Thioesterase.1"
                     /locus_tag="ctg70_1"
                     /protein_end="749"
                     /protein_start="519"
                     /score="124.9"
                     /tool="antismash"
                     /translation="PPLFCVHPAGGLSWCYAGLMTNIGTDYPIYGLQARGIGLREELPK
                     TLDDMAADYIKQIRTVQPKGPYHLLGWSLGGNVVQAMATQLQNQGEEVSLLVMLDAYPN
                     HFLPIKEAPDDEEALIALLALGGYDPDSLGDKPLDFEAAIEILRSDGSALASLDETVIL
                     NLKNTYVNSVGILGSYKPKTFRGNVLFFRSTIIPEWFDPIEPDSWKPYINGQIEQIDID
                     CRHKDLCQ"
     CDS_motif       1563..1592
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg70_1_0004"
                     /evalue="3.70E-04"
                     /label="NRPS-beforete1"
                     /locus_tag="ctg70_1"
                     /protein_end="530"
                     /protein_start="520"
                     /score="13.3"
                     /tool="antismash"
                     /translation="PLFCVHPAGG"
     CDS_motif       1743..1817
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg70_1_0005"
                     /evalue="4.00E-14"
                     /label="NRPS-te1"
                     /locus_tag="ctg70_1"
                     /protein_end="605"
                     /protein_start="580"
                     /score="44.8"
                     /tool="antismash"
                     /translation="QPKGPYHLLGWSLGGNVVQAMATQL"
     CDS             2328..2537
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1009:mbtH-like protein (Score: 107.4; E-value:
                     3.7e-33)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg70_2"
                     /transl_table=11
                     /translation="MANPFENADGTYLVLINEEGQYSLWPGFIDVPSGWTVVHEQKGRE
                     ACLDYIQSHWSDMRPNSLKTVENV"
     CDS             complement(2581..3258)
                     /locus_tag="ctg70_3"
                     /transl_table=11
                     /translation="MAVQQYGVLKGIVLDMKRETDDDSPHFQVKMLGEENTYYRCAINV
                     MSSSEESEVLYLADDQFDSGSITILPNMPYGYTRINEANREVALDYVRGNLFDPREMKP
                     LPHEITGPDNDLNDFIETYMKKAQDEKAPVYIFGSKFGPEQAADKIFGFTPTNGMHNIH
                     MNQGNAMDTRWKKDNGSWHDGGILIQFADQWAAVFLAFLSQSWCTDENGNPVRDCDHTQ
                     TPA"
     CDS             complement(3370..4335)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg70_4"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 5.7e-12, bitscore:
                     33.4, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADPKQVWDAEMVMKVKEPLPEEYVYFRKGLVLFTYLHLAAEPEL
                     AQALKDKEVTAIAYETVSEGRTLPLLTPMSEVAGRMAAQIGAQFLEKPKGGKGILLAGV
                     PGVSRGKVTIIGGGVVGTNAAKMAVGLGADVTIIDLNADRLRQLDDIFGHQIKTLISNP
                     VNIADAVAEADLLICAVLIPGAKAPTLVTEEMVKQMKPGSVIVDVAIDQGGIVETVDHI
                     TTHDQPTYEKHGVVHYAVANMPGAVPRTSTIALTNVTVPYALQIANKGAVKALADNAAL
                     RAGLNTANGHVTYEAVARDLGYEYVPAEKALQDESSVAGA"
ORIGIN
        1 atgcacagcc tgcgtttatc atgacgaaca ctaaagctgc aaatcacatt ccgccagttg
       61 aaaacgtgcc gaaaatcgtg ctggatgatc ccgagctggc agagaaacta aacacgtacc
      121 ctgcagagaa tccaaaaaat aaggatagga ctcagccgct ttcgcctttg aatacggcgt
      181 atgtcattta tacatccggc tcaacaggtg ttccaaaagg tgtgatgatt cctcaccaaa
      241 atgtaacacg tttatttgca gcgaccgagc actggttccg tttcagctca gacgatatct
      301 ggaccatgtt ccattcctat gcgtttgatt tttcggtgtg ggagatctgg ggtcctctgc
      361 tgcatggcgg acgtcttgtg atcgtgcctc accacgtcag cagatcgcca gaagcgtttc
      421 tgcgcctgct tgtcaaagag ggtgtgactg ttctgaacca gacaccgtct gctttctatc
      481 agtttatgca ggcggaacgg gaacagccgg atctcggaca ggcactcagt ctgcgctatg
      541 tcatttttgg cggagaagcg cttgaactca gccgtttaga ggattggtac aaccgccacc
      601 ctgaaaatag accgcagctt atcaatatgt atgggattac agaaacgacg gtgcatgtga
      661 gctatatcga attagaccga agcatggcag ctttacgggc aaacagcttg atcggctgcg
      721 gcattcccga tcttggtgta tacgtgctgg atgaacgcct gcagccggtc ccgccgggtg
      781 ttgccggaga gctgtatgta tcaggagcgg ggttagcacg aggttatcta ggccggcctg
      841 gtttaacatc tgaacgcttc atagctgatc cgttcggtcc tccaggcacc cggatgtacc
      901 gcactgggga tgtggcccgc ctccgcgctg acggttccct cgactatgtc ggccgtgcag
      961 accatcaagt gaaaattcgg ggattccgaa ttgaattggg agaaattaaa gccgcacttg
     1021 ttcagcatcc gcagcttgaa gatgcggcag tgatcgttcg tgaggatcag ccgggagata
     1081 aacggttggc agcttatgtc atcccttcgg aagaaacatt tgatacggcg gaactgcgca
     1141 aatatgctgc ggagagactg ccggattaca tggtgcctgc tgcctttatg acgatgaaag
     1201 aattgccgct gacaccgaat ggaaagcttg atcgtaaagc gctgcccgct ccggatttcg
     1261 cagcggcagt gacaggacga ggaccgagaa cgccgcagga agaaattctc tgtgacctct
     1321 ttatggaggt gcttcacttg ccgcgtgtcg gcattgatga ccgtttcttt gatttaggcg
     1381 gacattcctt gcttgctgta cagctcatga gccgcattcg tgaagcgctc ggcgttgaac
     1441 tcagcatcgg caacttgttc gaagcaccta ctgtagccgg gctcgctgaa agacttgaaa
     1501 tgggatcaag ccaaagcgct cttaacgttc tgctgccgct ccgaacaagc ggagacaagc
     1561 ctcctttatt ctgtgttcat ccagcagggg gactcagctg gtgctatgcc ggtttgatga
     1621 cgaatatagg aacggattat ccgatctacg gcttgcaggc acggggaatc gggctgcgcg
     1681 aggagcttcc gaaaacactt gatgacatgg ctgccgatta cattaaacaa atccgaacgg
     1741 ttcagccgaa aggcccgtac catctgctag gctggtctct gggcgggaat gtcgttcaag
     1801 cgatggccac ccagctgcaa aatcaaggag aagaagtttc cctgttggtg atgctggatg
     1861 cctatccaaa ccacttcctt cctataaaag aggcgccgga tgatgaggaa gcgcttattg
     1921 cccttctggc gttaggcggg tacgatccgg acagcttagg ggacaaaccg ctagattttg
     1981 aagcagcgat tgagattctt cgcagtgacg gcagtgcgct tgcgagcttg gatgaaacgg
     2041 tcattttaaa tttgaagaat acgtatgtca attctgtcgg cattttaggc tcctataagc
     2101 cgaaaacctt ccgtggaaat gttttgtttt tcagatcaac aatcattcct gaatggtttg
     2161 atccgattga acctgattcg tggaagcctt atattaacgg acagattgaa caaatcgaca
     2221 tcgactgccg tcataaagat ttatgtcagc cggaacctct ggctcaaatc ggcaaggttc
     2281 tcgctgtcaa actggaagag ttgaataaat aagggaggag aaatagaatg gcaaatcctt
     2341 ttgaaaatgc ggatggcacg tatttggtgc tgatcaatga agaaggccaa tattccctat
     2401 ggcccggttt tatagatgtg ccgagcggct ggacagtcgt tcatgagcaa aaagggcgtg
     2461 aagcttgttt ggactatatc caatcgcatt ggagcgatat gaggccaaac agcctaaaga
     2521 ctgttgaaaa tgtgtaatcg tatcaaaaag agactgctcg ccgcagtctc tttttctatc
     2581 ttacgcaggc gtttgggtat gatcacaatc tctgaccggg tttccgtttt catctgtaca
     2641 ccaagattgg gataaaaacg ctaaaaacac agcggcccac tgatctgcaa actgaatcag
     2701 aatacccccg tcatgccaag agccgttgtc ttttttccag cgggtatcca ttgcattccc
     2761 ttgattcata tggatattgt gcattccgtt ggtcggggta aagccgaata ttttatctgc
     2821 agcctgttca ggtccgaatt ttgaaccgaa tatataaacc ggcgctttct catcttgtgc
     2881 tttcttcata taggtttcaa taaaatcatt taaatcatta tcaggtcctg tgatttcatg
     2941 aggcaaaggc ttcatttccc gcgggtcaaa caaattgccc cgcacataat ccagtgccac
     3001 ttcacggttt gcttcattga tccttgtata tccatacggc atgttcggaa ggatggtaat
     3061 tgagcctgaa tcaaactgat cgtcagccaa atacaatact tcagattcct cagaactgga
     3121 catcacattg atggcgcacc tgtaatacgt attctcttca ccgagcattt tgacttggaa
     3181 atgaggactg tcatcatctg tttcccgctt catgtctaag actatgcctt ttaacacgcc
     3241 atattgctga acagccatat gaatcctcct ttatgatcaa cttcatagtc ataattcgtg
     3301 aaatggtctc ttttttcctt ttaaaaaata aaaaagcctg cagaaatctt tctgcaagct
     3361 cattgtgaat taagcacccg ccacagatga ttcatcctgt aaagctttct cggcaggaac
     3421 atactcatag cctagatctc ttgctacagc ttcataggtc acgtgtccgt ttgcggtgtt
     3481 taaacccgct ctcagtgccg cattgtctgc gagcgctttt actgcccctt tgttcgcgat
     3541 ttgcagcgcg tatggaacag taacgttagt cagggcgatt gttgacgtac gagggactgc
     3601 gcctggcatg ttcgctacag cataatgcac aaccccgtgt ttttcatatg ttggctgatc
     3661 atgtgttgtg atatggtcga cagtttcgac gatgccgcct tggtcgatcg ctacatcaac
     3721 aatgaccgaa ccaggtttca tttgttttac catttcctca gtgacaagag tgggagcttt
     3781 agcacccgga attaataccg cgcaaatgag gagatccgct tccgccacag catcagcaat
     3841 attgaccgga ttagaaatta acgttttaat ctgatggccg aagatgtcat caagctggcg
     3901 caagcggtct gcgtttaagt caatgatcgt cacatctgca ccgaggccga cagccatttt
     3961 cgccgcgttt gtcccgacaa cgcctcctcc gataatcgtt acttttccgc gggaaacgcc
     4021 cggcaccccg gcaagcagaa tgccttttcc gcctttaggc ttttctaaga attgagcgcc
     4081 gatttgcgcc gccattctgc ccgcaacctc tgacattggg gtcagaagag gcaatgtccg
     4141 gccttcactg accgtttcat atgcgatggc agttacttct ttatccttca aggcctgtgc
     4201 aagctcaggc tcagctgcta aatgaaggta cgtaaacagc acaagtcctt tgcggaaata
     4261 aacatattct tccggcagcg gttcttttac cttcatgacc atttcggcgt cccagacctg
     4321 tttcggatca gcaat
//
