LOCUS       c00027_gnlPro..         5773 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KCLBPBIC_27.
ACCESSION   c00027_gnlPro..
VERSION     c00027_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 22:31:29
            Original ID  :: gnl|Prokka|KCLBPBIC_27
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5773
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5773
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1908:4881]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1909..4881
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5773
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5773
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..296
                     /locus_tag="ctg27_1"
                     /transl_table=11
                     /translation="ISHSLRMIFNNKNATRESGKKSLAQWYAKVGEFGDENFNTVAATI
                     YERQGEILNYFINRSTNASAESLNSKIKQFRAQLHGVIDVKFFLFRLSKIFG"
     CDS             complement(279..1307)
                     /locus_tag="ctg27_2"
                     /transl_table=11
                     /translation="MKSTFKVLFYLKKGSEKKNGEVMIMARITIDGKLCQFSTKQSILP
                     ENWNIVAGKAKGKDAGRINALLEDIKASLNNIYHEQQRRDNYVTAEKVKNEFLGHSEKH
                     ETILDLFKKHNDDVKQLVGISKTIATYRKYEVTRRHLAEFIQSKYNISDIAINEITPMF
                     ITDFELYLRTTCKCSYNTTAKFMQFFKRIILIARNNGILIGDPFANYKIRLEKVDRGYL
                     TEDEIKIILKKKMVSERLENVRDLFVFSCFTGLAYIDVANLTQENVRKSFDGKLWIITK
                     RQKTNTDVNVPLLDIPKMILKKYKGKLPNGKILPVISNQKLNAYLKEIGGTCKNLCLAK
                     YL"
     CDS             1909..4137
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_3"
                     /sec_met_domain="Gly_radical (E-value: 2.6e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             4153..4881
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.3e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGEYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(4953..5399)
                     /locus_tag="ctg27_5"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDEDGKAADTRTSRQKEAMHRLVSELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIEEFES"
     CDS             5616..5771
                     /locus_tag="ctg27_6"
                     /transl_table=11
                     /translation="MNEKFSSMEQTISEQGADISRLYRIIDQKDKEIHVLKKRLSKYEG
                     PDKDSHN"
ORIGIN
        1 caatctcgca ctctttgcgc atgattttca ataacaaaaa cgccacaagg gaaagcggga
       61 agaaaagcct cgcccaatgg tatgcaaaag tcggagagtt cggagacgaa aacttcaaca
      121 ctgttgcagc aaccatctat gaacggcagg gagagattct caactacttc atcaatcggt
      181 ctaccaatgc gtctgctgaa tctcttaatt caaagatcaa gcagtttcga gcacaactac
      241 atggggttat agatgtaaag ttcttcttat tcagactctc aaagatattt ggctaaacac
      301 aggtttttgc aagtgccccc tatttctttc aagtaggcgt taagtttttg attactgatt
      361 accggaagta tcttaccgtt cggcagcttg cccttatact tcttcaatat cattttggga
      421 atatccagca agggaacatt tacgtccgta ttggtctttt ggcgtttggt gattatccat
      481 aacttaccgt caaaggattt gcgaacattc tcttgggtaa ggttggcgac atcaatataa
      541 gccaaacctg tgaaacagga aaagacaaac aagtccctga cgttttccaa ccgttcggaa
      601 accatcttct ttttaaggat gatttttatt tcgtcctctg tcaaataccc tctatccact
      661 ttctccaaac ggattttata attggcgaac gggtcgccaa tcaagatgcc gttattacgg
      721 gctatcagga taatgcgttt gaagaactgc ataaacttgg cagttgtatt ataactgcat
      781 ttgcaggtag tacgcaaata caactcgaag tctgttataa acatcggagt gatttcattg
      841 atggcaatat ccgatatatt gtatttgctt tggataaact cggcaaggtg gcggcgggtc
      901 acttcgtatt tacgataggt ggcaatcgtc ttggatatgc cgaccaactg tttcacatcg
      961 tcattgtgct ttttgaaaag gtctaaaatg gtttcgtgtt tttcactgtg tcccaaaaat
     1021 tcattcttca ccttttcggc tgtcacatag ttatcacgcc gttgctgttc gtggtagata
     1081 ttattcaagg atgcttttat atcctccaat aaggcattta ttctgcctgc atccttacct
     1141 ttggctttac ctgctactat attccagttt tcgggcagga tgctttgttt tgtactgaat
     1201 tggcacaact taccgtcaat ggtaattctt gccataatca taacctcacc gttcttcttt
     1261 tcagaacctt tctttaaata gaaaaggact ttaaatgtac tcttcataac ctcacatttt
     1321 taagttgcaa aactactaat aacttattaa atatgagata gatagacggt ggacaaattc
     1381 ggactttctg cggtcatttc gggacaaatc gttaccgttt ttgtccggta gggaataggt
     1441 aacgatttag taacgaaact atgtcttttc aagtcctttt gttgtcttgg aaacaagact
     1501 tataggcaat aaaaaaacct acaaatcgct gatttgtagg tttttgtcct cttactgtcc
     1561 tcttttgtcc agtaggttct gcggagagac agggattcga accccgggta cctcgcagta
     1621 caacggtttt caagaccgcc gcaatcgacc actctgccac ctctccaaaa ctcctttatc
     1681 agaagtgctt ttcgttaaaa gcggtgcaaa ggtacgaaga tattttaaac ttgcaagagt
     1741 tttcacaaaa aaaaatcacg aacatcattt ccaaagaata gcaatatcta ggtattttat
     1801 gttgaaaagc ataaaataga gcatatttat gctgattata tgaaaaaaac gttgtaactt
     1861 tgtgcgctct aaagaaaagg ataactacaa cctctaattt tatcatttat ggaattaaat
     1921 aagatcttta aagacggtct ttggagcact gaaatcaatg tcagagattt cgtaagtcac
     1981 aacatcactc cgtattatgg agatgcttct ttcctcgaag gacctaccga acgcacaaaa
     2041 gctgtatgga accgctgcct cgaagcactg gcagaagaaa gagccaataa cggagtacgc
     2101 tcactggata atgtcaccgt ctcaaccatc acttctcata aagccggata tatcgacaaa
     2161 gaaaacgaac tgatcgtcgg tctgcaaaca gacgaacttc tgaaacgtgc aatcaaacct
     2221 ttcggaggta tcaacgtggt gagcaaagcc tgccatgaaa acggcgtgga agtggacgac
     2281 cgtgtaaaag atatattcac tcactaccgc aagacgcaca atgacggagt atttgacgta
     2341 tataccgaag aaatccgttc gttccgttct ctgggattcc tcaccggact tcctgacaac
     2401 tatgcccgcg gacgtatcat cggcgactac cgccgtatgg ctctctatgg tatcgaccga
     2461 ttgattgagg caaaaaaaga agatttgcgt aaccttaccg gtccgatgac cgaagcccgc
     2521 atccgcctgc gcgaagaagt ggcagagcaa atcaaggcat taaaagacat gaaggtaatg
     2581 ggcgaatact acggactcga cttgagccgt cctgcctaca ccgcacaaga agcagtacaa
     2641 tgggtataca tggcttacct tgctgccgta aaagaacaag acggtgccgc catgtcactg
     2701 ggtaacgtct cttcgttcct cgacatttat atggaatatg aattgagcaa aggaaccatc
     2761 acagaatctt tcgcacagga gctgatcgac cagtttgtca tcaaactgcg tatggtgcgt
     2821 cacctgcgta tgcaatctta caacgacatc tttgccggcg acccgacatg ggtaaccgaa
     2881 tccctgggcg gacgcctcaa cgacggacgt accaaggtga caaagacttc tttccgcttt
     2941 ctgcaaacac tctacaacct cggcccttca ccggaaccga acctgactgt attgtggagc
     3001 ccggaactgc cggaaggatt caaagaattc tgcgcaaaag tatctatcga cacttcttcc
     3061 atccaatatg aaaacgacga cctgatgcgt gaagtgcgcc agtctgacga ttacggaatc
     3121 gcatgctgcg tatcttatca ggaaatcgga aaacaaatcc aattcttcgg cgcacgttgc
     3181 aacctggcaa aagccctgtt gcttgccatc aacggcggac gttgcgaaaa caccggtacg
     3241 gtaatggtga aaaatatccc cgtactaacc agcgacacac tgaacttcga agaagtaatg
     3301 agcaactaca agaaggtatt gacagaaatc gcccgtgttt acaacgaagc gatgaacatc
     3361 atccattata tgcatgataa gtattactac gaaaaagccc aaatggctct tgtagatacc
     3421 aacccacgca tcaaccttgc ttatggtgta gccggacttt ctatcgccct cgattcactg
     3481 tcggccatca aatatgccaa agtgaccgca cgccgcaacg acataggtct gacagaaggt
     3541 ttcgatattc aaggcgaatt cccttgcttc ggtaatgaca acgacaaggt agaccacctg
     3601 ggtgttgacc tggtctattt cttcagcgaa gaattgaaga aacttccggt ttacaaaaat
     3661 gcccgtccta ctttatcttt gctcaccatc acttccaacg tgatgtatgg caagaagaca
     3721 ggtgctactc ccgacggacg cgccaaaggt gtcgcttttg ctccgggagc caaccctatg
     3781 cacggacgtg acaagaacgg tgccatcgcc tctttgagtt ccgtagcgaa attacgttac
     3841 cgcgactcac aggacggtat cagcaacact ttctccatcg tccccaaatc actgggagct
     3901 actgaagagg atcgcgtaga aaatctggtg acaatgatgg atggctattt caccaaaggc
     3961 gctcaccacc tgaacgtgaa cgtactgaac cgtgaaatgc tttacgatgc catggaacat
     4021 ccggaaaaat atccgcaact caccatccgt gtttccggtt atgccgtaaa cttcgtgaag
     4081 ttgagccgcg aacatcaatt ggaagttatc agccgtagct tccacgaacg tatgtaatca
     4141 gatctactca atatgacgat aaacgtacat tcatacgaaa gtatgggaac attcgacggg
     4201 ccgggcttac ggctcgtcgt ttttcttcaa ggatgcaatt tccgctgcct gtattgtgcc
     4261 aaccccgaca cgatagccgg aaaaggaggc acacctaccc caccggaaga aatcgtccgc
     4321 atggctatga gccaacgtcc ttttttcggg aaacgcggag gagtcacttt ctccggagga
     4381 gaacctacgt ttcaggccaa agcactagtc cctttagtgc gtgaactgaa agaaaaggga
     4441 atacatgtat gcatagacag caacggcggt atctggaacg aagaagttga agaacttttc
     4501 aaactgacgg atcttgtatt gctggatatt aaagagttca atccggcacg ccatcaagca
     4561 ctgaccggaa gaagtaacga gcaaaccatc cgcacggcag catggctgga agaaaacgag
     4621 aaacctttct ggctgcgtta tgtattagtg cccggataca gcgatttcga ggaagacatc
     4681 cgccgtttag gagaagctct gggagaatat aagatggtgc aacgagtgga aatcctgcct
     4741 tatcatacat taggcgtaca taagtatgaa gcgatggagc aggaatacaa gctgaaagac
     4801 gtaaaagaaa atacccccga acaacttgaa aaagccgcgg aggtatttaa agagtatttc
     4861 actactgtgg tggtcaatta gttgaaaaag ttgagaattg agagttaact atgcagtata
     4921 acagtgcgca ggcaactctc aactctccat tctcaactct caaattcttc aatcacctcg
     4981 aaacagggac actctttgat ccgctcccaa gggtcaacaa ttccattata attagtatca
     5041 ggactcaagt cccggtgacc gaccaccttt gcatccggat aacgctgcaa caactcgctt
     5101 accagacgat gcatcgcctc tttttggcgg gaagtacgcg tatccgctgc ctttccgtcc
     5161 tcatccagtc cgccttcata gcacacccct aaactaatcg cattaaaacc gcgtgcatga
     5221 gcacccaccg tatcttcatc cctcatcggc tctatctgtc ccgacttgcg aatgtagtaa
     5281 tgataccccc aacacgaaaa gccccgaaag cgatgggcag tgtccacatc cttcgccgtg
     5341 aagtcgcgat cagcacgggt agcagagcag tgaatcacaa tcagactaat ctttctcatc
     5401 gtccttctcc ctttccgttg ttttcacatt acacacttcc ttagtaacta ctcaggtatc
     5461 ttttcctgaa aattgaaaaa gtaatccaaa ctctcttacc tgttgaaatg tatctgataa
     5521 ctttatgcca atcccctttc tctattatcc cctagatatt acttttgcat tcaatgattg
     5581 agcaggtaac agacatatct aagttcttat cggagatgaa cgagaaattc tcttcgatgg
     5641 agcaaactat atctgagcaa ggtgctgata ttagccgtct ttatcgtatt attgatcaga
     5701 aggataaaga gattcatgta ttaaagaaac gtttatccaa atatgaagga cctgataagg
     5761 atagtcataa tag
//
