LOCUS       c00164_gnlPro..        15538 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KCLBPBIC_164.
ACCESSION   c00164_gnlPro..
VERSION     c00164_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 22:31:29
            Original ID  :: gnl|Prokka|KCLBPBIC_164
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15538
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15538
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3968:8828]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3969..8828
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..15538
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15538
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             2..310
                     /locus_tag="ctg164_1"
                     /transl_table=11
                     /translation="RGYISKNVQLDLFETANIRLEVPYRLNQKDWSPTFIPFAKARKRI
                     ETDFSQLCDQFMIVRNYAKDTVGLFTRILGKISAFTILQYINHINNKPIGRLKYALI"
     CDS             476..1039
                     /locus_tag="ctg164_2"
                     /transl_table=11
                     /translation="MKISFSKQTKERAFKQLYEEYYVPFCLYAKRFVDDKETREDIVSD
                     VFTSLWDKLDTDSFDLQSDTALAYIKMCVKNSCLNFLKHQEYEWSYAENIQKKAPVYET
                     EPDSVYTLDELYRMLYETLDKLPENYRTVFIKSFFEGKTHVEIAEEMNLSVKSINRYKQ
                     KTMELLRNELKDYLPLLLLLFHRA"
     CDS             complement(1625..2761)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025:AMP-binding enzyme (Score: 55.8; E-value: 1e-17)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg164_3"
                     /sec_met_domain="AMP-binding (E-value: 2.4e-18, bitscore:
                     55.8, seeds: 145, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding_C (E-value: 1.9e-09, bitscore:
                     28.5, seeds: 272, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFNRQQQCLLLDGKEYTSEDIRQLIAGGTEAHSPAYWDLYLFLN
                     EWFNDSPVITVHTSGSTGTPKELMVRKDRMMQSARLTCEFLNLKKGESALLCMNLRYIG
                     AMMVVVRSLVAGLNLLVRPASGHPMADIKESLRFVAMVPLQVYNTLQVPEEKERLKQTD
                     ILIIGGGAVDETLETEIKHLPTAVYSTYGMTETLSHIALRRLNGASASEHYYPFPSVKL
                     SLSAENTLVIDAPLVCDETLQTNDIARIYPDGSFMILGRKDNVINSGGIKIQAEELEKL
                     LRPFIPVPFVITSVPDPRLGQAVTLLIVGQLDTREIENKLQTTLEPYYRPKHIFITESI
                     PQTENGKIDRVGCRALAASYLSHSHQFPSIELHDDAIM"
     CDS             complement(2794..3822)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MR_MLE_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg164_4"
                     /sec_met_domain="MR_MLE_C (E-value: 9.4e-29, bitscore:
                     90.5, seeds: 401, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNCKIEIIPRLLHFKQPAGTSRGTYTTRKVWYLHFTSPDFPGRVG
                     IGECAPLPALSCDDLPDYEDILMKVCRQVEREQGILDMDGLCDYPSILFGLETAIRHFF
                     AGNWALCDTAFSRGETGIPINGLIWMGDYDCMLSQIEKKMEAGFRCIKLKIGAINFEEE
                     LALLRYIRSHFSSKEIELRVDANGAFSPVDAMEKLKRLSELDLHSIEQPIRAGQWEEMA
                     RLTSASPLPIALDEELIGYNTLERKQELLSTIRPQYIVIKPSLHGGMCGGDEWITEAEK
                     RHIGWWVTSALESNIGLNAIAQWCATFNNPLPQGLGTGLLFTDNVEVPLEIRKDCLWFC
                     KG"
     CDS             complement(3969..4793)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg164_5"
                     /sec_met_domain="ECH_1 (E-value: 4.9e-82, bitscore: 264.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWTTIREYDDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALYMGLVNKVVPLEQLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATLLYYLTDEAQEGKNAFLEKRKPNFKQYPKFP"
     CDS             complement(4797..6464)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg164_6"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.7e-08, bitscore:
                     23.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTDKKNILQLVALLEAHGITKVVLCPGSRNTPIVHTLSNHPNFT
                     CYPVTDERSAGYFAIGLALNGGKPAAVCCTSGTALLNLHPAVAEAFYQNVPLVVISADR
                     PAAWIGQMDGQTVPQPDVFRTLVKKSVNLPEIHTEEDEWYCNRLVNEALLEMTHHGKGP
                     VHINVPVFEPLFQFTADSLPAVRVITRYQGLNVYDRDYNDLIDRMNKYQKRMIIIGQMN
                     LIYLFEKRYIKLLYKHFAWLTEHIGNQTVPGIPVKNFDAALYAMPEEKVGQMVPELLIT
                     YGGHVVSKRLKKFLRQHPPKEHWHVSPDGEVVDLYGSLTTVIEMDPFEFLEKIASLLDN
                     RTPEYPRVWENYCKIIPEPDFAYSEMSAVGALLKALPESCALHLANSSVVRYAQLYSIP
                     STIEVCCNRGTSGIEGSLSTAVGYAAASDKLNFIAIGDLSFFYDMNALWNINVRSNLRI
                     LLLNNGGGEIFHTLPGLDMSGTSHKFIAAVHKTSAKGWAEERGFLYLQAQNDEELAEAM
                     KTFTQPEAMEQPVLLEVFTNKNKDARMLKNYYHQLKQK"
     CDS             complement(6499..7599)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg164_7"
                     /sec_met_domain="Chorismate_bind (E-value: 3.3e-57,
                     bitscore: 183.9, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDEEISNLTTIDAFIQRKQPFAVYRIPGEKVPRLLTQAEGEVRL
                     IYDLKELNGQRGFVIAPFQVSERCPVVLIQPDQWGQPLLVDDDTEEDRKVALRMQGQES
                     FLTSFTEEYAACFHTFINALRDKTFDKLVLSRHLTIDKVADFSPSSIFRAACKRYIHSY
                     IYLCYTPQTGIWLGSTPEIILSGEKDEWNTVALAGTQPLQDGRLPQVWDEKNRKEQDYV
                     ASYIRRQLLSLGIHSTENGPYPAYAGALSHLKTDFRFSLKDNKGLGDLLKVLHPTPAVC
                     GLPKEEAYRFILQNEGYDRRYYSGFIGWLDPEGRTDIYVNLRCMHIEDKHLTLYAGGGL
                     LASSELNDEWLETEKKLQTIKRLITH"
     CDS             complement(7596..8828)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg164_8"
                     /sec_met_domain="HBT (E-value: 2.3e-18, bitscore: 56.5,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDAKEISKRTLAALLKVQQMGVRIVLASGR
                     PTYGLMPLAKMLELGNYGGFILSYNGCQIINAQNGEILFERRINPEMLPYLEKKARKNG
                     FAIFTYHDDTIITDSPENVHIQNEARLNNLQIIKEEEFSAAVDFAPCKCMLVSDDEEAL
                     IGLEDHWKRRLNGALDVFRSEPYFLEVVPCSIDKANSLGALLEVLNMKREEVIAIGDGV
                     CDVTMIQLAGLGVAMGHSQDSVKACADYVTASNEEDGVAVAVEKAILSEVRAAEIPLDQ
                     LNAQARHALMGNLGIQYTYADEDRVEATMPVDHRTRQPFGILHGGATLALAETVAGLGS
                     MILCQPDEIVVGMQVSGNHISSAHEGDTVRAVGTVVHKGRSSHVWNVDVFTSTNKLVSS
                     IRVVNSVMKKR"
     CDS             complement(8913..9275)
                     /locus_tag="ctg164_9"
                     /transl_table=11
                     /translation="MIQVRIKRVYEDFSETDGYRVLVDKLWPRGMKKEWLKYDYWAKDI
                     TPSPTLRKWFHEDIPGHWGDFVTQYQKELDASPAMTDFLILIKPHPVVTLLYASKEPVY
                     NHARILRDYLEMRLKE"
     CDS             complement(9340..11097)
                     /locus_tag="ctg164_10"
                     /transl_table=11
                     /translation="MRFWVCIFVLLFVHNQFSRADKIINNDSLYTEKYIRDIYISNPKR
                     ALQLLDEAESRRAFPLRLINELRSLSYRNMYMNKLAFMYARKSYLLDSISQREPKHMLK
                     MTVYLAELSSLMSKYNESMQYALNGIVQAQKLKDREAEARLLFCIGENNWRLSLKDEAY
                     NYFDRTIELLRGSKDVREMMLLSYYYGTEMGFLMTDSRIDEALALAYEREKLLKKLEKV
                     PEVPEGYIDGQYSYLYAKLAYISYLEKKYAQAEKYYQQYLAMKESHTPDGKMYSIPYLI
                     LSKQYEKVLDNSKDFKELLRTQRDTLNAQYLAVLNKEVQAYWGLNRYKEAAEIRETIIA
                     ITDSINSNDRKNAALELNAMYGASEKEEYIAEQASQLKIRNVSLCFLACIVVLTLFILW
                     RLWRFNHIIEYKNRMLAKLINEKFANKKDGNQLLEVYEEQEVSSELEPELISPEEQDEL
                     LDETDKESGEEEENKKIFQELNHTVVQKQLYLSPELSREDLAQIVHLNNARFARMIREC
                     TGTNFNGYINGLRINYAIKLMKKYPNYTIRAIADESGFNSAPILYNLFKKKTGMTPYEF
                     KKAQDTLRN"
     CDS             complement(11276..12019)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acetyltransf_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg164_11"
                     /sec_met_domain="Acetyltransf_1 (E-value: 3.5e-09,
                     bitscore: 27.0, seeds: 223, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENNYITLTKENIANEHICCAFSDKKCKDSYELKKEWLKKEFDNG
                     YVFRRIDERAKVFIEYGPAEKAWVPVNAPNYLMINCFWVSGKYKGCGHGKALLQSAIDD
                     AKSQGKDGLVTVVGTSKFHFMSDTKWLLRQGFQTIEKLPYGFSLLSLKLNPAAPDPSFN
                     DYVLSGECPDKDGIVVYYTHRCPFTEFHVRGSLVSAAASKDIPLRIIELESMEQAQNAP
                     TPVTIFTLFYNGKFVTTDLSVCLEG"
     CDS             complement(12075..13004)
                     /locus_tag="ctg164_12"
                     /transl_table=11
                     /translation="MAKKGELHIRNKHNGQYDFPLLMENYPPLKRFVSLNPLGIQTINF
                     FNPQAVKALNKALLVTYYGIRYWDIPKQYLCPPIPGRADYIHYIADLIQPNGTTPDLPA
                     GDANGQKSKCRCLDIGVGANCIYPIIGHTEYGWTFVGTDIDPVSIENARKIVTCNPVLA
                     HKIDLRLQKDSKKIFDGIIMPDEYFDVTICNPPFHSSREEAEDGTLRKLSSLKGTKINK
                     VQLNFGGSANELWCEGGEVRFLLNMISESQKYQKNCGWFTSLVSKEKNLEKLYAKLKSV
                     NVSEYKVIRMQQGTKSSRILAWRFSTNS"
     CDS             complement(13077..13727)
                     /locus_tag="ctg164_13"
                     /transl_table=11
                     /translation="MNTDITFIIADNQDITRMGMHGYISAIFSGCRMIDVTDKKELMLA
                     LVECNDSVVILDYTLFDINGIEEFLIIEKRFPRVRWILFSNELSEDFIRRMSIERNIGM
                     ILKENSGEEIHSALMCTAHGERFLCHQITNLLIIGSDKPEIHSVLTVTEIDILKLIAHG
                     KSVKEIALERTSSIHTIITHKKNIFRKLGVNNVYEATKYTLRAGLIEMVEYYI"
     CDS             complement(13797..15203)
                     /locus_tag="ctg164_14"
                     /transl_table=11
                     /translation="VVAISLSSCDDELTDINRNPNATENPQPAYLLAAAQYHAANLYWG
                     SSTNYNSTLLWVQHWAKIQYTEPDCYNVDNGSFTTTWDTGYATLITDLNAIISSELGND
                     NYRGIATVWRSWVYLLLTNLYGNIPYSEYAQSVTPSYDSQETVLRGLLEELIAADQLLS
                     TTGGTVEGDLIYGNDITRWKQLANSLRLRIALELADRDEDTARRVIASLWDNRNSVIDS
                     NDAIARFVFTSSPQWNPWASAFNSRDDQRVSKTLIDKLNEWNDPRIGILAQLPQDESVK
                     NYVGAANSLSADAANNQGFNKVSRPGTYFLKDSSPAVFYTYAEVLFIFAESAARGWITA
                     DAETLYREAITASLNQFGIIDNRIIDSYLQQEAIRFDAAHWYESIGWQKWIAYYGQGPD
                     AFTDWRRLGYPQLIPGPDSVLGSGELPRRFFYPVTEQSLNGKQYQIAISHQGADEITTR
                     LWFDVANKER"
     CDS             15351..15536
                     /locus_tag="ctg164_15"
                     /transl_table=11
                     /translation="MFQDKYVFAQLTAFLNRSKFNRIVAKYDGDKYVKFFTCWNQLLTL
                     MFGQLCNRESLRDLIVA"
ORIGIN
        1 tagaggatat attagtaaga atgtgcaact ggatttgttt gagactgcaa atataagact
       61 tgaagttcca tatagactga accaaaagga ctggagtcct acattcatcc catttgctaa
      121 agccaggaaa agaatcgaaa cggatttctc acaactatgc gatcagttca tgatagtgag
      181 aaattatgct aaagatacag taggattgtt taccagaata ctcgggaaaa taagtgcatt
      241 tacaatcctt caatatataa accacattaa caacaaacct attggcagac ttaaatatgc
      301 gcttatttaa ttccgccaac gggtaataca attatcctat atacaaaagg acagtctcat
      361 ccgattattt tttattcatt agagttgaaa gcaaaaaaag aatcgaaaat taggctttga
      421 taattatctt attatatatt tgcagcaaaa tgaaaaacta cagaacctca aaataatgaa
      481 aatatctttt tccaaacaga ctaaagaacg ggctttcaaa caactttatg aggagtatta
      541 cgtcccattt tgtttgtatg caaaaaggtt tgttgatgat aaagaaacac gtgaagacat
      601 cgtttcggat gtattcacct cattatggga taaactggat actgattctt ttgatcttca
      661 atcggacact gcattagctt acatcaaaat gtgcgtaaaa aatagctgcc tgaatttcct
      721 gaaacatcag gaatatgaat ggagctatgc ggaaaatata cagaaaaaag ctcctgtcta
      781 tgaaacagaa ccggatagtg tatatactct tgacgaactg tacagaatgt tgtatgaaac
      841 attggacaaa cttccggaga actaccgtac agtatttata aaaagcttct ttgaaggcaa
      901 aacgcatgta gaaattgcag aagagatgaa tctcagtgtg aaatctatca accgttacaa
      961 gcaaaagaca atggaacttt tgcgcaatga actgaaagac tatctgccat tgttactttt
     1021 gttattccat cgcgcataac agccacttct tatcaacaca tacaaactat tacttgtcca
     1081 tccggaaagt attaatagta aagttgttat tgcctaatag caaacttcca atcctttata
     1141 tataaagctg ctttcgacta atcataaact ataaatgcaa gcttcgtttt tataatcaaa
     1201 ggttgcattt ataaaaatga aacttgcaat tatataatca aagcttgcaa ttatagttta
     1261 taataggtga aaaacaagtt tactattaac cccaaacaag tttatctata atgaagaata
     1321 actttgctat taatgattta ggcgtaaaat agcagtagat tgttatacaa ctgctatgag
     1381 aaataacgag aatgagtatg agaaatgcct atttttaggg ataaatcaca ggaaaagtat
     1441 gagaaatgcc aacttttaaa agtatttctc atattctatt acattatgtt tcaacaggtt
     1501 gacttatatt catgagaata tgagaaatag aattgccaaa tcatttatgg gaggcgctga
     1561 attcaagttg taaatgcgac acctccttat tttacaagtt cactagtttt accggaaacc
     1621 tcaactacat tattgcatca tcatgtagtt ctatggatgg gaactgatgc gaatgggaaa
     1681 ggtatgatgc tgccaaagcg cgacatccga cacgatctat tttgccgttt tccgtctgtg
     1741 gaatagattc tgtaataaaa atatgtttgg gacgataata tggttccaat gttgtctgca
     1801 acttattttc tatctctctt gtgtccaatt gtcccactat caacaaagtc acagcctgcc
     1861 ctaaacgcgg gtcgggaaca gaagtgatga caaatggcac aggaataaac ggtcggagca
     1921 acttctccag ttcttccgcc tgaatcttaa tccctccgct attgattaca ttatcttttc
     1981 gccccagaat cataaaactt ccatcgggat agatacgtgc aatgtcattg gtctgcaatg
     2041 tttcgtcaca taccaaagga gcatcgatga ctaatgtatt ctctgcggat aaagacaatt
     2101 ttactgaagg aaatggataa taatgttctg aagccgatgc tccattcaaa cgacgaaggg
     2161 caatatgcga caaggtttcg gtcatgccat aagtggagta aaccgccgtc ggaagatgtt
     2221 tgatctccgt ttccagagtc tcatcgacgg ctccgcctcc tataatcaga atatccgttt
     2281 gttttaatcg ctctttttct tcgggaacct gcaatgtgtt ataaacttgc aatggaacca
     2341 ttgcaacaaa tctcaaagat tcttttatat cagccattgg atgaccggaa gcgggacgaa
     2401 caagcaggtt caaacctgcg accaaagaac gtaccactac catcatggca ccgatataac
     2461 gaaggttcat acataataat gccgactcac ctttcttcaa attcagaaac tcacaggtca
     2521 ggcgcgcact ttgcatcatc cggtccttac gtaccatcag ttctttaggt gtacccgtag
     2581 agcctgatgt atgaaccgta atgaccggag aatcattgaa ccattcgttc aggaaaagat
     2641 ataaatccca ataagcagga gaatgagctt ccgttcctcc tgctatcaat tgacgtatat
     2701 cttcagaagt gtattctttt ccgtccagta acagacattg ttgttgtcgg ttgaatatca
     2761 tcacaacatc ctttaaatat catcctttaa atattatcct ttgcaaaacc acaagcaatc
     2821 ttttcttatt tcaaggggca cttccacatt atccgtaaaa agcaatcctg tccccaatcc
     2881 ctgtggcaac ggattattga atgttgcaca ccactgtgca atagcgttca aaccgatgtt
     2941 tgattccaga gcggaagtga cccaccagcc gatatgtcgc ttttcagctt cggtaatcca
     3001 ttcgtcccct ccgcacattc ctccgtgaag ggaaggttta ataacaatat attgcggacg
     3061 gatggttgaa agcagttctt gttttctttc caacgtgttg taccctatca attcttcatc
     3121 caaagctatc ggtaatggag atgcggaagt aaggcgtgcc atttcctccc attgtccggc
     3181 acggatcggc tgttcgatag agtgcagatc aagttcggac agacgcttca gcttttccat
     3241 ggcatcaacc ggagaaaaag caccattcgc atctacacgc aactctattt ccttagagga
     3301 gaaatgtgaa cggatataac gtagcaatgc caactcttct tcaaagttga tagcaccaat
     3361 ctttagtttg atacaacgga atccggcttc catctttttc tcaatctggg ataacataca
     3421 atcataatct cccatccaga taagaccgtt gatgggaatc cccgtctctc cacgtgagaa
     3481 agccgtatca cataaagccc agtttcctgc aaagaaatgc cggatggccg tttccagacc
     3541 aaataaaatg gaaggatagt cgcacaaacc atccatatcc aatattcctt gttctctctc
     3601 cacttgccgg caaactttca ttaatatatc ttcgtaatca ggaagatcat cgcaactcaa
     3661 agcgggcaaa ggtgcacact ctcctattcc cactctgccc ggaaagtctg gagaggtgaa
     3721 atgaagatac cagactttac gcgtagtata tgttccccgg gaagtccctg ccggttgctt
     3781 gaaatgaagc aaccggggaa tgatttctat tttacagttc attgtgaata aattctctat
     3841 tcaccacaga gtaacataga gccccacgaa gttttagtaa tcagatctct tgcagttctc
     3901 ctgcagccct cttacagttc tgctttgcag aaaaaataaa ctctgtgtta ctctgtagtg
     3961 aaatcggttt aagggaattt cggatattgt ttgaagttcg gtttacgttt ctccagaaat
     4021 gcattctttc cttcctgtgc ttcatccgtc aggtaataga gcaacgttgc atcgcccgcg
     4081 agttcctgaa tacctgcctg gccatccagt tcggcattca atcccgcttt aatcatgcgc
     4141 aaagccaacg gactaagcaa catcatttct tccgcccact gcacatattc atcttccaac
     4201 tgttccaagg ggaccacctt attcactagt cccatataca gtgcttcctg cgcattgtat
     4261 ttccggcaga ggaaccaaat ttcacgtgct ttcttttggc ctaccacacg tgccagataa
     4321 gacgcaccga atccggcatc aaagctaccc acacgaggac cagtctgacc gaagatggca
     4381 ttttccgaag cgatggataa atcacatact acgtgaagta catgtccgcc accaatggca
     4441 aagccgttca cggcggcaat cacaggtttc ggaatactac gaatctgctt ctgtacatcc
     4501 agtacgctca aacgaggcac accgtctttc ccgatatatc cgccacgtcc tttcacattt
     4561 tggtcgcctc ccgaacagaa tgccttatct ccggctccgg taatcacaat cacgtcaata
     4621 tccgcttctt cacggcaaat acgcaatgca tcactcattt cggcggtagt agtcggggta
     4681 aatgcattcc gataacgttc acggttgatt gtgatacggg caattccatt ataataatca
     4741 aaaagaatat cgtcgtattc tctgatggtt gtccactctc tttgtgttga cataatctat
     4801 ttttgtttta attgatgata ataattcttc aacatacgcg catccttgtt tttgttggta
     4861 aacacttcca gcagcactgg ctgttccata gcctccggtt gggtgaaagt tttcatcgct
     4921 tcagccagtt cttcatcatt ttgcgcttgc agatagagga agccacgttc ctccgcccag
     4981 cctttggccg acgttttatg gactgctgca ataaatttat gtgaggttcc cgacatatcc
     5041 aatcccggta aagtatggaa aatctctcct cctccgttat ttaataaaag gatacgcaag
     5101 ttagaacgga cgttgatatt ccacaacgca ttcatatcat agaagaaact taaatctccg
     5161 atagctataa aattcagttt atcggaagct gccgcatagc cgactgccgt agacagtgaa
     5221 ccttcaatgc cacttgttcc ccgattgcaa cagacctcaa tcgtggaagg gatcgaatac
     5281 aactgcgcat agcgaaccac cgaactgttt gccagatgca aggcgcacga ctccggcaag
     5341 gctttcagca acgcaccgac agccgacatc tccgaataag caaaatccgg ttcggggata
     5401 atcttgcaat aattctccca tacacgtgga tattcaggag tccggttatc cagcagactg
     5461 gcaatcttct ccaaaaactc gaacggatcc atctcgatta ccgtagttaa tgagccgtac
     5521 aaatcgacca cctcaccgtc cggcgatacg tgccaatgtt ctttaggcgg atgctggcga
     5581 aggaacttct tcaaccgttt ggaaaccacg tgtccaccat aagtaatcag caattcggga
     5641 accatctggc ctaccttctc ttcgggcatg gcataaagcg ccgcatcaaa attcttcacc
     5701 ggaataccgg gaactgtctg gttgccgata tgttctgtca accaggcaaa atgtttatat
     5761 aataacttga tatatctttt ctcaaacaga tagataagat tcatctgacc gatgataatc
     5821 atacgcttct gatatttatt catccggtcg atcagatcgt tataatcacg gtcgtagaca
     5881 ttcaatccct gataacgggt aatgacacgc actgcgggta gtgaatcggc cgtaaactga
     5941 aacagcggtt cgaaaacagg tacattgata tgtaccggac ctttcccatg atgggtcatc
     6001 tccagcaaag cctcattcac cagacgattg caataccact catcttcttc cgtgtgaatc
     6061 tccggaaggt tcaccgattt cttgaccaac gtccgaaata cgtctggttg cggaacggtc
     6121 tgtccatcca tctgtccgat ccaggcagcg ggacggtctg cagaaatgac gactaaagga
     6181 acattctggt aaaatgcttc cgccacagcc ggatgaaggt ttaataacgc tgtaccggaa
     6241 gtgcagcaga cagcagcagg cttaccacca ttcaacgcaa gaccaatggc aaaataaccg
     6301 gcactccgtt catcagtcac cggataacag gtaaaattcg ggtggttaga caaagtatgc
     6361 acgatgggcg tgttacggct acccggacac aacacaactt tggtaatccc atgcgcctcg
     6421 agcaacgcaa ccaactggag tatgttcttc ttgtctgtat acatgacggt taatgattag
     6481 tgaattagtg attaatgatt aatgggttat aagccgtttg atggtttgca acttcttttc
     6541 cgtttccagc cattcatcat tcaattccga agaggccaat aatccgccgc cggcataaag
     6601 agtgaggtgt ttgtcctcga tatgcataca gcgcagattc acgtagatgt cagttctccc
     6661 ttccggatcc agccatccga taaagccgga ataatagcgc cggtcgtaac cttcgttctg
     6721 caatataaac cgataggctt cttcctttgg cagaccgcat acggcaggcg tcggatgcaa
     6781 tactttcaga agatcgccca aacctttatt atctttcaat gagaagcgga aatctgtctt
     6841 taaatgagac aaggcaccgg cataagccgg ataaggcccg ttttccgtag aatggatgcc
     6901 caatgaaaga agctggcggc gaatgtaaga ggctacataa tcctgttctt tccggttctt
     6961 ttcatcccat acctgcggca gcctgccatc ttgcaaaggc tgcgttccgg ctagcgcaac
     7021 tgtattccat tcatctttct cacctgataa aatgatttcg ggagtacttc ccagccaaat
     7081 acccgtttgg ggagtgtagc ataaatatat ataggaatga atataacgct tgcaagccgc
     7141 acgaaaaatg gacgaaggag aaaagtctgc tactttatca atagtaagat gacgggaaag
     7201 cactaattta tcaaaagtct tatcgcgtaa agcattgata aaggtatgaa agcacgctgc
     7261 atattcctcc gtgaaagaag tcaggaatga ttcttgtcct tgcatccgca aagcgacctt
     7321 gcggtcttct tccgtatcat catcaaccag taaaggctgc ccccactgat cgggttgaat
     7381 caacacaacc ggacacctct cactcacctg aaacggagcg atgacaaatc ctctctgtcc
     7441 attcagttct ttcagatcgt atatcagacg tacctctcct tccgcctgtg tcaacagacg
     7501 gggaactttc tccccgggaa tacggtaaac agcaaatggc tgcttccgct gaataaatgc
     7561 atcaatagtt gtcaagttac ttatttcttc gtcggtcatc tctttttcat cacactgttg
     7621 actacccgta tggaagacac cagtttattg gttgaagtaa acacgtctac attccacaca
     7681 tgggatgaac gtcctttatg tacaactgtc cctactgcac gtaccgtatc tccttcatgc
     7741 gcagaagata tatgatttcc gctgacctgc attcctacta caatttcgtc cggctggcaa
     7801 aggatcatcg aaccgagtcc ggcaactgtt tctgccaacg caagagtagc tccaccatgt
     7861 aaaataccaa aaggctgacg ggtacgatga tctaccggca tcgtcgcttc cacacggtct
     7921 tcatcggcat atgtgtattg aattcccaag tttcccatta acgcgtggcg tgcctgcgca
     7981 ttcagttggt caagggggat ttcggccgca cgcacttcag aaaggatcgc tttctccact
     8041 gcaacagcca ccccgtcttc ctcattcgaa gctgtcacat aatcggcaca ggccttcacc
     8101 gagtcctgtg aatgtcccat agctacaccc aatcctgcca attgaatcat ggtcacatca
     8161 cacacgccgt cgccaatagc aatcacttcc tcacgcttca tatttaagac ttccagcaaa
     8221 gcccccaaag aattggcttt atcaatggag cagggaacga cttccagaaa ataaggttcc
     8281 gaacggaata catccaacgc tccgttcagt cttctcttcc agtgatcttc caaaccgatc
     8341 aatgcttctt cgtcgtcact gaccagcata catttgcaag gagcaaagtc aacggctgca
     8401 gaaaattctt cttccttgat tatttgcagg ttattcaacc gggcttcatt ttggatatgt
     8461 acattttcag gagaatccgt tatgatcgta tcatcatgat aagtgaatat ggcaaaaccg
     8521 tttttacggg ctttcttttc cagatacggc aacatttcgg gattaatccg acgttcaaac
     8581 agaatttctc cgttttgtgc attgataatc tgacagccat tataggaaag gataaaacct
     8641 ccatagtttc caagttcgag catcttggcc agcggcatta ggccataagt cggtctgccg
     8701 gacgccagca caatacgtac tcccatttgc tgaaccttca acagggcagc caatgtacgt
     8761 ttactaattt ctttcgcatc attgaggagt gttccgtcta catcaagaac caataattta
     8821 tacttcatga ttacacgatt ttgatttaga ttacaaaggt aggaaattga gtgaacgaca
     8881 gaaagcgtaa acagataaaa attactttaa tttcactctt tcagacgcat ttccagataa
     8941 tcgcgaagga ttcgggcatg attatacaca ggttctttag aagcataaag taaagtgacc
     9001 acaggatggg gtttgataag aattagaaaa tcagtcattg caggagaagc atccagctct
     9061 ttttgatact gcgtgacgaa atctccccaa tgtccgggaa tatcttcatg aaaccactta
     9121 cgtaatgtag gggatggtgt tatgtctttt gcccaataat cgtatttcaa ccattctttc
     9181 ttcattccgc gtggccataa tttatctacc aacacccggt atccatctgt ttctgaaaaa
     9241 tcttcgtaaa ccctctttat ccttacctgt atcatttcca ttaatattta gttatcaata
     9301 gataaaccgg gaaaaacgaa aattgtttag tagaactatt taattcctca atgtgtcctg
     9361 cgctttttta aattcataag gagtcattcc tgtctttttc ttaaaaaggt tatataaaat
     9421 tggagcgcta ttaaatccgg actcatcagc gatggcacgt atagtatagt taggatattt
     9481 tttcattagt ttaatcgcat aattaatgcg cagtccgttg atgtacccat tgaaattagt
     9541 tccggtgcat tctcttatca ttcgggcaaa acgtgcatta ttcagatgta caatctgtgc
     9601 caaatcttct cttgagagct ctggagagag atatagctgt ttctgaacga ctgtatgatt
     9661 taattcctgg aatattttct tgttctcctc ttcttcccca ctttctttgt ctgtttcgtc
     9721 caaaagttca tcctgttcct ctggagaaat caattcaggt tccagttctg atgaaacctc
     9781 ctgttcctcg tacacttcca ataactgatt accatctttt ttattggcaa atttctcgtt
     9841 tatcagtttg gcaagcatcc tgtttttata ctcgattata tggttgaaac gccacaaacg
     9901 ccaaagtata aacagggtca gcactacaat acacgccaaa aagcagagtg aaacattcct
     9961 tatttttagc tgtgaggctt gctcggcgat gtattcttct ttttcggaag caccgtacat
    10021 cgcgttcaat tctaatgcag catttttcct gtcgttactg ttaatgctat ccgttatcgc
    10081 aatgatggtt tcacgtattt ctgctgcttc tttataacga ttcaggcccc aatatgcttg
    10141 cacttcctta ttaagtacag caagatattg agcgttcaag gtatctcgtt gtgtcctcaa
    10201 cagttcttta aagtccttac tgttgtctag tactttctca tattgtttgg aaagaatcaa
    10261 ataaggaatc gaatacatct tgccatccgg agtatgagat tctttcattg ccagatattg
    10321 ttgataatat ttttctgctt gcgcatattt cttttctaaa taagagatat aggccaattt
    10381 tgcatataaa tagctatatt gaccatcaat gtaaccttcg ggaacctccg gtactttctc
    10441 taatttttta agaagttttt cgcgttcata agcaagagcc aaagcttcat caatacggga
    10501 atctgtcatt aagaatccca tctctgttcc ataataatag gaaagcagca tcatttctct
    10561 cacatccttt gacccacgca gtaattcaat cgtacggtca aaataattat acgcctcatc
    10621 tttcaatgac aacctccaat tattctcccc tatgcaaaaa agtaatctgg cttccgcctc
    10681 tctgtctttc agcttttgcg cctgcacaat tccattgaga gcatattgca tactctcatt
    10741 atatttactc attaaagatg aaagttcagc caaataaacc gtcatcttca acatgtgctt
    10801 gggttctctt tgagagatag aatcaagcaa gtatgacttc cttgcataca taaaagctag
    10861 tttattcata tacatattac gatatgacaa actgcgcaat tcattaatca gacgcagagg
    10921 gaaggctctt ctggattctg cctcatccaa taactgtaaa gcacgtttgg ggttggatat
    10981 ataaatatct ctgatatatt tttctgtata taaagagtca ttatttataa ttttatcagc
    11041 cctcgaaaat tgattgtgta caaatagaag cacaaaaata catacccaaa aacgcataaa
    11101 actcttaatc gtgtttaatc attaatgatc caaaggtatg tattttctat agaacgtaca
    11161 gacacaattt aaatttgcaa aataaaaata agaaacatta gctaaacata ttagagatgt
    11221 aacgtggttc caaatcagct attcatctga atcacagccc caacgcttta cccaatcacc
    11281 cttcaaggca cacacttaag tcagttgtta caaacttccc attataaaaa agagtaaata
    11341 tagtaaccgg agtaggtgcg ttttgcgcct gctccatact ttctaattca attattctca
    11401 atggaatatc tttgctcgca gcagcactga ctaaagaacc gcgaacgtga aactccgtaa
    11461 acgggcagcg atgggtataa taaacgacta ttccatcctt atccggacac tcaccgctca
    11521 acacgtaatc attaaaagaa ggatcaggag cagccgggtt tagtttcaaa cttaaaaggc
    11581 taaatccgta agggagtttt tctattgttt ggaaaccttg tcgcaacagc catttcgtgt
    11641 cactcataaa atgaaattta gaagtgccca ccactgtgac aagcccatct ttcccctgcg
    11701 attttgcatc atcaatggca gactgcaata atgccttacc atgtccgcaa cctttatatt
    11761 tacctgacac ccagaaacag tttatcatca gataattggg agcattcacc ggaacccatg
    11821 ctttttcagc aggaccgtat tcgataaaca cttttgcccg ttcatcaata cgtctgaaca
    11881 catagccgtt atcgaactct ttcttcagcc attctttttt gagttcataa ctatccttgc
    11941 atttcttatc tgaaaaagca cagcatatat gttcgtttgc aatattctcc tttgtcaaag
    12001 tgatgtaatt gttttccata atatcctatt taattaatag gttaaacaaa taacagttcg
    12061 atattcactc attgttatga gttggtagaa aatcgccaag ctaatatcct gctactttta
    12121 gttccctgct gcattcgaat aactttatat tcggacacat tcaccgattt taatttagca
    12181 tataactttt ccagattttt ttctttagaa accaaactcg taaaccatcc gcagttcttc
    12241 tgatattttt ggctctcgga aatcatattc aataagaaac gtacttctcc gccctcgcac
    12301 caaagttcat tcgcactccc gccgaaattt agctgcactt tatttatctt cgtacctttc
    12361 agactgctta atttgcgtaa cgttccgtct tccgcctctt cacgcgaact atgaaacgga
    12421 ggattgcata ttgtcacatc aaaatactca tcaggcataa taattccatc gaaaatcttc
    12481 ttactatctt tctgaagtcg caagtcaatc ttatgagcca acacagggtt gcaagtcact
    12541 atctttcgtg cattttctat cgaaaccgga tcaatatcag taccaacgaa ggtccatcca
    12601 tattcagtgt gtcctatgat cggataaatg caatttgcac ctaccccaat atccagacat
    12661 ctacatttag acttctgtcc attagcatct cctgccggta aatccggtgt tgttccgttc
    12721 ggctggataa gatcggcaat ataatgaatg taatccgccc ttccgggaat aggcggacac
    12781 aaatattgct tcggtatgtc ccaataccgg atgccataat aagttaccaa taatgctttg
    12841 ttcaaagcct tcaccgcttg cggattaaaa aagtttatag tttgtattcc gaggggattg
    12901 agcgatacga atctctttaa tggaggataa ttttccatca acaatggaaa atcatactga
    12961 ccattgtgct tatttcttat atgtaactca cctttttttg ccatgcgggc aaagatacag
    13021 agaattttcc tgcctacaaa ttcttcttaa ctcttatcct aacactcttc tgaatatcat
    13081 atataatatt ctaccatttc aatcagcccg gcgcgtaaag tatacttagt tgcttcatat
    13141 acattattca cacccaactt ccggaaaata ttctttttgt gggtgataat agtatgaatg
    13201 cttgaggttc tttccaatgc tatttctttc acagatttcc catgtgctat caatttaaga
    13261 atgtcaatct ctgtaacagt caaaacagaa tgaatttccg gtttgtcgga accgattatc
    13321 aataaattgg ttatctgatg acaaagaaat cgttccccgt gtgctgtaca catcaatgca
    13381 gaatgaattt cctcaccgga gttctctttc aaaatcatac caatattcct ttctatactc
    13441 atcctacgaa taaagtcttc gctcaattca ttggaaaaca atatccaacg aactctcgga
    13501 aaacgttttt ctataataag aaactcttct ataccattga tgtcaaacag tgtatagtca
    13561 agtataacga cactgtcatt acattctacc aatgcaagca tgagctcttt cttgtctgtc
    13621 acatctatca tgcgacatcc actgaatata gcggaaatat atccatgcat tcccattctg
    13681 gttatatcct gattatctgc tatgataaat gtaatgtcag tgttcattgt tattctgatt
    13741 tttaaattca acggatattt ttcaaacgca tattgcagcc cggagacacg gaggtatcat
    13801 cgttctttat tagctacatc aaaccacaaa cgggtagtaa tctcatctgc tccctgatgg
    13861 gatatggcaa tctgatactg cttaccattc agtgattgtt ccgttacggg ataaaaaaag
    13921 cgacgaggta actctccgga acccaaaacg gaatcgggac cagggataag ctggggataa
    13981 ccgagcctgc gccagtcagt gaaagcatcg ggtccctgtc cataataagc gatccacttc
    14041 tgccagccga tagattcata ccaatgtgcg gcgtcgaacc gaattgcctc ctgctgtaaa
    14101 taggaatcga ttatacgatt gtctataatg ccaaactgat tcaatgaagc agtaatagct
    14161 tcgcggtaga gcgtttccgc atcagccgtt atccagccgc gtgcagcgga ttctgcaaag
    14221 ataaacaaga cctcggcata cgtataaaat acagccggag agctatcttt caaaaaataa
    14281 gtaccgggac gggaaacttt attaaatccc tgattattag cggcatcagc agataaggaa
    14341 ttggcagctc ctacatagtt tttcacactt tcgtcttgag gtagctgggc taaaattcca
    14401 attctcggat cattccattc attcagttta tcaatcaacg tttttgatac ccgctgatca
    14461 tcacgtgaat tgaaagcaga tgcccatgga ttccattgtg gtgaagaagt aaaaacaaaa
    14521 cgggctatag catcattgct atctattaca ctgttacgat tatcccaaag ggaagcaata
    14581 acccgacgcg ctgtatcctc atcccggtca gctaattcaa gggcgatacg tagccgtagg
    14641 gagttggcca attgtttcca gcgggtaata tcatttccgt aaatcaaatc cccttctacc
    14701 gttcctcctg tggtagaaag caactggtca gcagcaatca actcctccaa aagcccgcgc
    14761 aaaacagtct cttgcgaatc ataagagggt gttacacttt gtgcatattc actgtatggt
    14821 atatttccat acagattggt caacaacagg taaacccacg aacgccacac tgtagctatc
    14881 cctcgataat tatcatttcc gagttccgaa ctgataatag cattcaagtc agttattagc
    14941 gttgcatagc cagtgtccca cgtagtggta aagctaccat tatccacatt atagcaatct
    15001 ggctctgtat attgtatttt agcccaatgt tgcacccaca acagagtaga attatagttc
    15061 gtactacttc cccaataaag attggcggca tgatactgtg cagcggcaag taaataagcc
    15121 ggctgtggat tttcagtggc attggggttg cggttgatat cagtcaattc atcatcacac
    15181 gatgataggg aaatggcaac cacttgtacc catattatat ataaaaaatc ataagcgtcc
    15241 actaaaaaac gcaaagagtt ctttgaaatg attgaatatt agtcttatac aacgattatt
    15301 cgagcgaacg gacttgattc tattactttg cattcttaat tgaatagcat atgtttcaag
    15361 ataaatacgt tttcgctcaa ctgactgcat ttctcaacag aagtaagttt aatcgaatcg
    15421 tagcaaaata cgatggagat aagtatgtga agttctttac ttgttggaat caactcctaa
    15481 cattgatgtt tggtcaactt tgtaatcggg agagtctacg tgacttaata gttgcaat
//
