LOCUS       c00180_gnlPro..         3835 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PJNHMONN_180.
ACCESSION   c00180_gnlPro..
VERSION     c00180_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 00:08:29
            Original ID  :: gnl|Prokka|PJNHMONN_180
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3835
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3835
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[705:3697]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      706..3697
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3835
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3835
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..388
                     /locus_tag="ctg180_1"
                     /transl_table=11
                     /translation="IHKKCPLSDKQFYFFNCLPKREHTIRGNGFVCSSLNELNSNPLTF
                     CSILFIVRILIRLTEVSGMNHMKYKTLIADRLGNSCLDAGFFISDKIIFYPISLIRSNF
                     KSLLSHVLKKMFFQTCSKERKIKL"
     CDS             706..2958
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg180_2"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERIGNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             2945..3697
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76.2;
                     E-value: 1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg180_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-33, bitscore:
                     103.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMTEENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDNKYHKVLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIDPPTKERVENAKKILAI"
ORIGIN
        1 tatccataaa aaatgccccc tttcagataa acagttttat ttttttaact gtctacctaa
       61 aagggagcat accattcggg gcaatggctt cgtctgtagt tcgcttaatg aattaaatag
      121 taaccccttg acattttgtt ctattttgtt tatagttaga atacttatac gactgacgga
      181 agtaagtgga atgaaccaca tgaagtataa gactcttata gccgaccgtc tgggcaattc
      241 ttgtctggat gctggctttt ttatttctga taagataata ttctatccaa tatccctgat
      301 aagaagtaac tttaaatcgt tattaagtca cgtgctaaag aagatgtttt tccaaacatg
      361 ctctaaagag cgaaaaataa aattatagtt gtatgcttgt cattattgta cttatatcag
      421 tacatttgta cgatttgata atattttatc aacgctctaa aaccctttat tttaaaggct
      481 ctacaagcat ttttgtgtat atgttcaaaa aaaagaacac ataatatcaa aaaattgttt
      541 gacgattgcc aaaaatcatg ttaataatgt tgttagatga ttaaaactta aataagttaa
      601 aactaatcat agtaaatcat ctgaacaatt ttgttgattt tttagcaaac atatttcaaa
      661 accttgactt aaggattttt ttaacaaaca gggaggtcaa ttaaaatgtt acaaaaagaa
      721 caatggcagg gatttaatgg acgaatctgg agagaagaaa ttaatttaag agagttcatc
      781 caggataact acactccata tgatggagat gcttctttcc tcgaaggccc taccgaggct
      841 actgacacac tgtggggcga actgcagaag ctgcagaaag cagagcgtga aaagggtggt
      901 gttcttgata tggatactga tatcgtatca agccttactt cccataaacc gggttacatt
      961 tctgagtctt taaaagagaa agaacagatc gtaggtcttc agacagataa acctttaaaa
     1021 cgtgcattta tgccatttgg tggtatccgt actgctgaag aagcttgtac aacttatggt
     1081 tacacaccaa atccagaatt ccacaagatt tttacagatt atcataagac acataaccag
     1141 gcagtatttg attcttatac tcctgaaatg aagaaagctc gtcacaataa gatcattact
     1201 ggtcttccag atacttacgg acgtggacgt atcgttggtg actaccgtcg tgtagctctt
     1261 tacggtattg acctgttagt tgctgacaag caaaaagatt tcgcaaactg cggtgacgga
     1321 acaatgacag atgacgttat ccgtttaaga gaagaaatct ccatgcagat taaagcatta
     1381 aaagatatga aagttatggc tgaaagctat ggctatgata tttccgaacc tgcttctaac
     1441 gcaaaggaag ctgttcaatg gacgtatttc ggataccttg cagcaatcaa gacacagaac
     1501 ggtgctgcta tgagtatcgg acgtatcgct actttccttg acatttatat caaacgtgat
     1561 atggataagg gaatccttac agagaaagaa gcgcaggaat taatcgacca tatgactatg
     1621 aagttccgta tggttaagtt cggacgtatc ccagcttaca accagctctt ctccggtgac
     1681 cctgtatggg caacacttga agttggcgga cttggacagg acggtcgttc catggttaca
     1741 aaaactgact accgtttcct ccacacatta gaaaatatgg gaccatcccc agagccaaac
     1801 ctcacagtac tttactctga gcgtcttcca aaagcattta gagattacgc agctcatatt
     1861 tctattacaa caagttcaat ccagtatgaa aatgatgatg taatgcgtcc agtatggggc
     1921 gatgattact ccatctgctg ctgtgtttct gctacacaga ctggtaagga aatgcagttc
     1981 ttcggagccc gtgcaaacct tgcaaaatgt ctcctctacg caatcaatgg tggtgtagat
     2041 gaaaagactg gtcagcaagt tggtccggaa tacaaaccaa ttacttccga atatcttgac
     2101 tatgatgaag taatgcacaa atatgatatc atgatggact ggttagcagg tttatatgta
     2161 aatacattaa atctcatcca gtacatgcat gacaaatatt actatgaata tgctttgatg
     2221 gccttaatcg atacaaatgt acgtcgtaca ttcgctacag gtatcgcagg attctcacac
     2281 gtagtagact ccttaagtgc tatcaaatat gcaaaggtta agacagttcg taacgaagaa
     2341 ggactcgttg tagattatga aacaactggc gacttcccta aatacggaaa tgatgacgac
     2401 cgcgccgatg atatcgctgt atggttactt cagacattta tgaagaaact ggagaagttc
     2461 catacttatc gtgattctga acctacaact tctatcctta ctatcacatc taacgtagta
     2521 tatggtaagg caacaggttc ccttccagat ggaagaaaag ccggtgaacc actttcccca
     2581 ggagcaaacc caagttatgg tgcagagcag aacggtctct tagcttcctt aaactctgta
     2641 gctaaacttc cttatgaatg ggcattagat ggtatttcca atacgcagac aatcaaccct
     2701 gatgcactcg gtcattccga agaagaacgt attggtaact tagtaaatgt tatggacggc
     2761 tacttcgcac agggtgctca ccacctaaac gtaaatgtat ttggtgttga gaaactgatt
     2821 gatgctatgg aacatccgga aaaagaagaa tacgcaaact ttacgatccg tgtttcaggt
     2881 tatgcagtta aattcatctc tctgacaaaa gaacagcagt tagatgtaat tgccagaaca
     2941 tgccatgaca gaatgtaata aaaatgcgat ggctcttgtt cattctacgg agagttttgg
     3001 ttcggtggat gggccgggag ttcgttttat tgttttttta cagggttgtc ctttgcgatg
     3061 tcagttctgt cacaatccag atacatggaa gatgacagag gaaaatggtg cggtgtggag
     3121 aagtgcggat gagctgttaa atcaggcact ccgttatcgt acttactgga aaaatagcgg
     3181 cggtattacc gtaagtggtg gtgagcctct tttacagatt gattttatgc tagagttttt
     3241 taaaaaggca aaagaaaaag gaatccacac tgttattgat actgctggag gaccttttac
     3301 aagagaggaa cctttctttt ctaaatttca ggaattgatg aaatatacag acctgttatt
     3361 agtggatatc aaacacattg ataacaaata ccacaaagtt ttaactggtc gtagcaatga
     3421 gaatattctt gatatgattc gctatctttc cgatatcaaa aaaccgattt ggattcgcca
     3481 tgtccttgtc ccggaacgaa gcgataaaga tgaatatttg actagattag ctgatttcat
     3541 tcactctctt gataatgtgg aaaaggtgga aatcctgcct tatcatacga tgggtattta
     3601 taaatggaaa gaacttggat tagaatatcc tttagaagga attgaccctc caacgaaaga
     3661 acgagtggag aacgccaaga aaattcttgc tatttgattt gccgacaaac agaatcctaa
     3721 aaccccccat atttgtgact ttgaagatag attgcgttga atggtatgct cccttttagg
     3781 tagacagtta aaaaaataaa actgtttatc tgaaaggggg cattttttat ggata
//
