LOCUS       gnlProkkaGHDGKICM_86   12923 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GHDGKICM_86.
ACCESSION   gnlProkkaGHDGKICM_86
VERSION     gnlProkkaGHDGKICM_86
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 17:02:17
            Original ID  :: gnl|Prokka|GHDGKICM_86
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12923
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12923
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[8814:11958]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      8815..11958
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..12923
                     /SMILES="NC(CS)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..12923
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             complement(191..415)
                     /locus_tag="ctg86_1"
                     /transl_table=11
                     /translation="MIDTVNGATSSAIIYSIAETAKENQLKPYEYFEYLLTEIPKHMDD
                     KNSNFLEELLPWSETLPENIRKPQKTDEK"
     CDS             complement(708..2432)
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 249.7; E-value:
                     9.8e-76)"
                     /gene_kind="transport"
                     /locus_tag="ctg86_2"
                     /transl_table=11
                     /translation="MFEMISRIYQIAGSSRKKITVGILCNILKSFFQSFMMLSVFLIML
                     NLNTLTSSIILKAVTIILISILGRFFFQWMSDRTMSGTGYDIFRDYRLEIGERLKQAPM
                     GYFSEQNIGTIQTILTTTIADLEGYSMMAIEQMTSGVAMAFLMSFMMFFFNPIIGTLSM
                     IGLVIGLTVLRMVRQRAAEHSPIYQAAQENLVNKCLEYIRGIAVLRSFSNGKSGQQEVH
                     TAFQKKWDADYSQEKATAGVLRLYGLVYKLMSCVLIAAAVILYMDGKISLPFCMTFLFC
                     AFTVYSDLETMGNSAFLSKKINTELDRLEEVTNIPQMDTTTDKLNPSNYEISLKDVSFG
                     YGSRRIIDHISLNIPEHTTCAIVGPSGSGKTTLCNLIARFWDVQEGSVCIGGQNVKDYT
                     ADSVLDCISMVFQKVYLFHDTIENNIKFGKPDASLEEVMEAAKRACCHDFIMALPDGYQ
                     TVIGESGSTLSGGEKQRISIARAILKDAPIVILDEATSSVDPENEQALLSAIEELTKNK
                     TLISIAHRLSTVQKADQIIVIDNGHVKQKGTHDQLSKKDGIYRNFLKLRVEATGWQL"
     CDS             complement(2433..4187)
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 258.5; E-value:
                     2.1e-78)"
                     /gene_kind="transport"
                     /locus_tag="ctg86_3"
                     /transl_table=11
                     /translation="MKSKEPGAIRQLFMFVGSSKGKMKFSIFMAVLGELFGMVPFLMLA
                     MLADSLYSGTATTKNTVILSGIAALCQCIKTFLTWRSSLLSHRISFTILQNIRKKIADK
                     MAKVPMGVMLETPSGNFKNLIVDNVAKLEDSMAHFMPELPSHITAPLCSILLIFILDWR
                     MGLASLITVPLGGLFFAAMMRGYASRMENYMCSANEMNSSLVEYVNGIQVIKAFNRSSS
                     SYGQYSKAVNYFHDCTMEWWSECWLWNAAARAVLPSTLLGTLPIGALLFMNGSITLPIL
                     MICLIVPLGFTGPLMKVSEAMEQVSMIKGNLEQVTVFLKTPELQRPVDPVTLTEPTFEF
                     SHVCFGYNKSEVLHDISFKTSPKSMTAIVGPSGSGKSTIAKLMAGFWDATSGTVLFGSQ
                     DIRNIPFEQLMGEISYVAQDNFLFDKTIRDNIRMGNPTATDEQIETVAKAANCHDFIMQ
                     LEKGYDTMAGDAGDRLSGGERQRITIARAMLKKASVVILDEATAYADPENEALIQEAIG
                     KLISGKTLIVVAHRLNTIRNADQILVVADGKIAGCGTQNELLENCPLYKKMWENYSDAV
                     TSKTKGES"
     CDS             4314..4931
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 73.3; E-value: 5.6e-22)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg86_4"
                     /transl_table=11
                     /translation="MSRDFQQTHENLIACAKKHFLEFGFERASIREICKDANVTNGAFY
                     NHFADKEALFGSLVESVVQTIQKIYSESIDKHFDLVKTDELKNLWKLSESTIIQIIEYI
                     YENFDVFRLLLMCSSGTKYAGFLDDLVRADVQETIKLIAELKARGVPVNDLDEDEWHML
                     VHSYYASIAEIVMHNYPKPAALKYTHTLSVFFSSGWQTVLGI"
     CDS             5010..5597
                     /locus_tag="ctg86_5"
                     /transl_table=11
                     /translation="MQKSNKLNGKDLINVGIYTAMTLVIFFVFGLLTALPIIYPFLLFI
                     WPFVCGIPMMLYYTKIQKFGMLTITGVICGLFFFLIGYTWIGLVGWTLGGILADIVLKA
                     GQYKSFKVTLLSYACFCLGMMGCPANLWIAGQSYWENIHSSMGDQYAEALQSMMPSWML
                     YAGFVILFVGGICGAVLGHKMLKKHFERAGIV"
     CDS             5597..6319
                     /locus_tag="ctg86_6"
                     /transl_table=11
                     /translation="MHMFDITEIKGFHLDPRTKLLLMAVVATAEFLYGHTAFMLAVALI
                     PFILLLTNRQYKAAIIFIVLFVAALVVKEIQNSIHFHVVVNMVVVLLVGLVLRLFPAFA
                     MGNYIIKSTTASECITSLSRMYIGRNITIPLSVLFRFLPTMQEESAAIKDAMRMREIQF
                     GTKKFWQNPMALLEYRFIPLMISVVKIGDELSAAALTRGLDNPAKRSSITKVGFTYYDA
                     IALVISGIMLFITLFIIK"
     CDS             6336..7835
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 154.1; E-value:
                     8.2e-47)"
                     /gene_kind="transport"
                     /locus_tag="ctg86_7"
                     /transl_table=11
                     /translation="MIEIKNVSFTYESGESENSLRNINLKIEDGETILLCGESGCGKTT
                     LTRLINGLIPHYYNGQLTGQIYLDGKEVKDYQLYQIASMVGSVFQNPRTQFYNVDTTSE
                     IVFGCENMGLSVQEMLARLENTTKRLKLENLLGRSLFALSGGEKQKIACASADAIYPNT
                     FVLDEPSSNLDISSIKDLTEVIRQWQSEKKTVIVAEHRLYYLAPYADRIIYMKRGEICN
                     EYTREEFLELDPKELKKMGLRALNPFDLMPEKKPEVNEKSLQIDNFWFSYEKRGYPIVN
                     IPNLTLPQGEIIGIIGDNGAGKSSFARCLCGLDKSSKGTLKLNGNSLNAKKRRHISYMV
                     MQDVNHQLFTEDVLDELLLSMDGENEEEDKVRAQEILAGLDLSDKLKLHPMSLSGGEKQ
                     RVAIGSAVASGKEIIIFDEPTSGLDYRHMLEVSEKLIQLKEMKKTLFLITHDPELIYKC
                     CTHLMFIEHGRILWHRPMDNEAVRLLQEFFSHEKGDSAMAI"
     CDS             7937..8083
                     /locus_tag="ctg86_8"
                     /transl_table=11
                     /translation="MIQAAVLFIIDGNVDLSKSLRQLFYKFVKKIFWEETYDHKKSVFS
                     ICV"
     CDS             8046..8810
                     /NRPS_PKS="Domain: Thioesterase (22-235). E-value: 7.7e-34.
                     Score: 109.9. Matches aSDomain:
                     nrpspksdomains_ctg86_9_Thioesterase.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004:thioesterase (Score: 190.4; E-value: 4.6e-58)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg86_9"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.3e-09, bitscore:
                     28.5, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIKNRYFPFVSENVADSNKGTILFCFHHAGGTATTYRPWTLKSN
                     DSVIIMCVELPGKGTRRTERMAVDFNEILPELSLQIADVSCGRKIVLYGHSMGAAMAFY
                     TAHYLWNQLDRKCEKVIVAGRQPPDQENPYEFKSYMNDDALIEELVRYNATPKEVLENK
                     ELLNFILPGLRQDYILNESLVYHGEVLDIPIVAHTGSQDYEANAEIMNLWKHMTTNIFQ
                     LNLFEGSHFFVTDLGNKYRDIVIQEAINKRED"
     aSDomain        8112..8750
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg86_9_Thioesterase.1"
                     /evalue="7.70E-34"
                     /label="ctg86_9_Thioesterase.1"
                     /locus_tag="ctg86_9"
                     /protein_end="235"
                     /protein_start="22"
                     /score="109.9"
                     /tool="antismash"
                     /translation="ILFCFHHAGGTATTYRPWTLKSNDSVIIMCVELPGKGTRRTERMA
                     VDFNEILPELSLQIADVSCGRKIVLYGHSMGAAMAFYTAHYLWNQLDRKCEKVIVAGRQ
                     PPDQENPYEFKSYMNDDALIEELVRYNATPKEVLENKELLNFILPGLRQDYILNESLVY
                     HGEVLDIPIVAHTGSQDYEANAEIMNLWKHMTTNIFQLNLFEGSHFFVTD"
     CDS_motif       8316..8372
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg86_9_0001"
                     /evalue="5.50E-05"
                     /label="NRPS-te1"
                     /locus_tag="ctg86_9"
                     /protein_end="109"
                     /protein_start="90"
                     /score="15.7"
                     /tool="antismash"
                     /translation="VLYGHSMGAAMAFYTAHYL"
     CDS             8815..11958
                     /NRPS_PKS="Domain: AMP-binding (152-550). E-value: 1e-87.
                     Score: 286.2. Matches aSDomain:
                     nrpspksdomains_ctg86_10_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (966-1034). E-value: 9.4e-17. Score:
                     52.9. Matches aSDomain: nrpspksdomains_ctg86_10_PCP.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg86_10"
                     /sec_met_domain="AMP-binding (E-value: 3.8e-96, bitscore:
                     311.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7.1e-15, bitscore:
                     45.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKENISIIKGMDTLRTIAEERKVSVNVLLKGIAVEIYYRWFANET
                     AIEYVTLNDLISEVIDRSKDKDTIVINDYVCSVMCNNDKAVLCIECEQLECDMIEGLSA
                     FTEEFVLEWADLADNAPIVHQIPRNQMAVRKNRNRTKKTFSDECLHHGFFENAANAPGK
                     AALIYYKNGNKEEISYFCLQDMILKLAGYLKENGVKSGTLVGVSIPKGINQVIAVYGIL
                     AAGATYVPIGVHQPVERKKKIIDTGRIRYIVTSNEMGSEEDLGVSVICLEAVLQKALPA
                     KVPVFPETNQPAYIIFTSGTTGIPKGVVISHSEAHNTIADINQRFNIGRNHTGIAISDL
                     DFDLSVYDIFGLLSAGGTLVVLSEETKREPVFWRKAIIDAGVNVWNSVPALFDMLLTTC
                     ETDKQMLPLQLVLLSGDWIKMDLYDRLKAISECCKFVSLGGATEAAIWSNYFVVNDIDS
                     GWKSIPYGEPLSNQYLRVVDGNGYDCPDYVNGELWIGGDGVAEGYLNDPELTDAKFVAI
                     DGVRWYKTGDLVRYNSAGIVEFLGRIDNQVKINGYRIELGEVENVIKRSPDVSNVVVGV
                     VDEKGKKELGAVIVPKINAAVNVDITCCEDNNKYSDFHMKERERIVRQLILEFCHMQSR
                     VSAEYRPIMEFWEGWLESHMEYSADIFSAREEMEPLQKVMNARNLFAEILTGEQRVEEL
                     LRREEVSPEFWSLKGEDTKYFLDKILAGDISNRKIAVLGARTGEIIKQYWDGFCQAEEI
                     TLFDTSAGILKLAQEKLGGMNANIRYCSTYNEGVEESELNKYDIVLAVNLLHTYEEPLK
                     EVKWAALLLKRKGDFYAIEYEELDPMGILISGLLENGFVNRKRGRREHTPLLLLDDWKN
                     IYGQAPFGEVAINRRGRLGALLIHAKNVTPEVIEMRSRILEYMNENLTAYMIPTKRMIV
                     KQVVLSKNGKVNRQQSLALLAVKQTAEKESIILQGIEADIAELWTNILSCKIFDRSQSF
                     FESGGDSLSATRFLAEIKKKYSVDIPLKDIFSTPSLKSVADMLQARLTENEGMVEGEI"
     aSDomain        9271..10464
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg86_10_AMP-binding.1"
                     /evalue="1.00E-87"
                     /label="ctg86_10_AMP-binding.1"
                     /locus_tag="ctg86_10"
                     /protein_end="550"
                     /protein_start="152"
                     /score="286.2"
                     /specificity="substrate consensus: Cys"
                     /tool="antismash"
                     /translation="FFENAANAPGKAALIYYKNGNKEEISYFCLQDMILKLAGYLKENG
                     VKSGTLVGVSIPKGINQVIAVYGILAAGATYVPIGVHQPVERKKKIIDTGRIRYIVTSN
                     EMGSEEDLGVSVICLEAVLQKALPAKVPVFPETNQPAYIIFTSGTTGIPKGVVISHSEA
                     HNTIADINQRFNIGRNHTGIAISDLDFDLSVYDIFGLLSAGGTLVVLSEETKREPVFWR
                     KAIIDAGVNVWNSVPALFDMLLTTCETDKQMLPLQLVLLSGDWIKMDLYDRLKAISECC
                     KFVSLGGATEAAIWSNYFVVNDIDSGWKSIPYGEPLSNQYLRVVDGNGYDCPDYVNGEL
                     WIGGDGVAEGYLNDPELTDAKFVAIDGVRWYKTGDLVRYNSAGIVEFLGRIDNQVKIN"
     aSModule        9271..11916
                     /complete
                     /domains="nrpspksdomains_ctg86_10_AMP-binding.1"
                     /domains="nrpspksdomains_ctg86_10_PCP.1"
                     /locus_tags="ctg86_10"
                     /monomer_pairings="Cys -> Cys"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       9472..9507
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg86_10_0001"
                     /evalue="6.70E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg86_10"
                     /protein_end="231"
                     /protein_start="219"
                     /score="10.2"
                     /tool="antismash"
                     /translation="GILAAGATYVPI"
     CDS_motif       9691..9750
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg86_10_0002"
                     /evalue="1.20E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg86_10"
                     /protein_end="312"
                     /protein_start="292"
                     /score="30.1"
                     /tool="antismash"
                     /translation="AYIIFTSGTTGIPKGVVISH"
     CDS_motif       9865..9900
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg86_10_0003"
                     /evalue="5.40E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg86_10"
                     /protein_end="362"
                     /protein_start="350"
                     /score="-1.6"
                     /tool="antismash"
                     /translation="GLLSAGGTLVVL"
     CDS_motif       10267..10356
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg86_10_0004"
                     /evalue="2.50E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg86_10"
                     /protein_end="514"
                     /protein_start="484"
                     /score="45.4"
                     /tool="antismash"
                     /translation="PDYVNGELWIGGDGVAEGYLNDPELTDAKF"
     CDS_motif       10429..10494
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg86_10_0005"
                     /evalue="6.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg86_10"
                     /protein_end="560"
                     /protein_start="538"
                     /score="37.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKINGYRIELGEVE"
     aSDomain        11713..11916
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg86_10_PCP.1"
                     /evalue="9.40E-17"
                     /label="ctg86_10_PCP.1"
                     /locus_tag="ctg86_10"
                     /protein_end="1034"
                     /protein_start="966"
                     /score="52.9"
                     /tool="antismash"
                     /translation="EADIAELWTNILSCKIFDRSQSFFESGGDSLSATRFLAEIKKKYS
                     VDIPLKDIFSTPSLKSVADMLQA"
     CDS             11983..12921
                     /NRPS_PKS="Domain: Heterocyclization (71-312). E-value:
                     3e-55. Score: 179.5. Matches aSDomain:
                     nrpspksdomains_ctg86_11_Heterocyclization.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg86_11"
                     /sec_met_domain="Condensation (E-value: 8.7e-30, bitscore:
                     93.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKTKEFIWLADNELRINLNKLASDGINVWLEDEKLKFKGEQGKV
                     TEEVLKWMRQNKTAIISYLLEKEEVQDKGFDLTPIQKAYFLGRDNLYELGGISANYYFE
                     IDMDKLDVKKFESIFNQVIHDNEALRGIILKCGRQAVLSNVPWFSIEVKRVNDEAEQKA
                     LRSEWESKIYPLESWPMFHVCLTTKDNCCYRLHVGFDCILLDAWSVTLMLKQIYKLYEN
                     DKIMVPAYTFREYCNEHGKLLNEKLKEQAERYWSQRCEMLPKAPALPYAKKLSEINIPH
                     FRRLNYELPKFDTETIYMKAKEYKVTPAAVLC"
     aSDomain        12196..12918
                     /aSDomain="Heterocyclization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg86_11_Heterocyclization.1"
                     /evalue="3.00E-55"
                     /label="ctg86_11_Heterocyclization.1"
                     /locus_tag="ctg86_11"
                     /protein_end="312"
                     /protein_start="71"
                     /score="179.5"
                     /tool="antismash"
                     /translation="DKGFDLTPIQKAYFLGRDNLYELGGISANYYFEIDMDKLDVKKFE
                     SIFNQVIHDNEALRGIILKCGRQAVLSNVPWFSIEVKRVNDEAEQKALRSEWESKIYPL
                     ESWPMFHVCLTTKDNCCYRLHVGFDCILLDAWSVTLMLKQIYKLYENDKIMVPAYTFRE
                     YCNEHGKLLNEKLKEQAERYWSQRCEMLPKAPALPYAKKLSEINIPHFRRLNYELPKFD
                     TETIYMKAKEYKVTPAAVL"
     CDS_motif       12562..12606
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg86_11_0001"
                     /evalue="9.00E-05"
                     /label="Cy3"
                     /locus_tag="ctg86_11"
                     /protein_end="208"
                     /protein_start="193"
                     /score="15.7"
                     /tool="antismash"
                     /translation="RLHVGFDCILLDAWS"
ORIGIN
        1 atgtaacatg taatgatgga gattgtcact tggaatttga ggtggcaatc tcttttttta
       61 tggattttat gcttatgtgc tgatgatagt ttttggtgta gtgtaacgat aaaagtaaaa
      121 ctgtgacgaa atatagtaaa cggtgaaaac tgagtacctt gaaaaatatc aggctgcttc
      181 tgcagccatt ctatttttca tccgttttct gtggctttcg tatattttct ggaagtgttt
      241 ctgaccaagg tagaagctct tccagaaaat tgctattttt atcatccata tgttttggta
      301 tctctgtcag cagatattca aaatactcat atggcttcag ctggttttcc ttcgctgttt
      361 cggctatact gtagatgatc gcagaactgg tggctccatt gacggtatcg atcatttccc
      421 agttcttctt tttatgtgct taagcacata aaaagaaaaa ctggcaactg caaatacttt
      481 gattagatag gaaaaataaa ttaaagagga atgtgtaggt tcaggcaatt gtttagtaaa
      541 aacaggtcta tgcaaaagcg aaaggtgagg tatatggcct gacgactgcc cggttctgga
      601 aggttaagag aaaatgagtg ccttggcaca aaaagaaaat cagggaagga attcattaaa
      661 tccttccctg gttttcattg atacagaggg gtgaaatggg gtaatattta taattgccat
      721 ccggtggctt ccacacgtaa tttaaggaaa ttccggtaaa ttccgtcctt tttactgagc
      781 tggtcatggg tgcccttttg tttgacgtgc ccattatcaa tcacaataat ctggtcagct
      841 ttttgtacgg tactcaatcg atgggctatg gaaattaaag ttttattttt agtcagctcc
      901 tcaatcgcag ataaaagagc ctgttcattt tcagggtcaa cactggaggt tgcttcatct
      961 aaaatgacaa tgggtgcgtc ttttaaaatt gcccgtgcaa tggagattct ttgtttttcg
     1021 ccgccagata atgtagatcc actttcgcca attacagtct gatatccatc cggcaaggcc
     1081 ataataaagt catgacagca ggctcttttt gctgcctcca ttacttcttc aagtgacgca
     1141 tcaggcttcc caaacttaat attattttcg attgtgtcat ggaatagata aaccttttgg
     1201 aaaaccatac tgatacagtc aagcacactg tcagcggtat agtctttcac attttggccg
     1261 ccaatacaca cagagccttc ctgaacgtcc cagaaacgag cgattaagtt acataatgtc
     1321 gttttaccgc ttcctgacgg acctacaatc gcacatgtag tatgctctgg tatgtttaag
     1381 gatatgtggt caataattct gcgggaacca tagccaaatg atacatcttt taatgaaatc
     1441 tcataattgg aaggattgag tttatctgtg gtggtgtcca tctggggaat atttgtcact
     1501 tcttccaaac ggtctaattc ggtatttatt ttcttactca agaatgcact gtttcccata
     1561 gtttccaaat cagaataaac ggtaaatgca caaaacaaaa atgtcataca gaatggtagg
     1621 gaaatttttc cgtccatgta taagataaca gcagcagcga ttaagacgca gctcatcaat
     1681 ttatagacaa ggccatatag ccggagaaca cctgcggttg ccttttcctg tgaataatca
     1741 gcgtcccatt tcttttgaaa tgcagtgtgt acctcttgct ggccgctttt gccgtttgaa
     1801 aatgaacgga gaacagcaat cccacgtatg tactctaaac atttattaac cagattttcc
     1861 tgggcagcct gataaattgg cgaatgttct gcggcccgct gtctgaccat gcgtaatact
     1921 gtcaggccaa ttactaagcc tatcatactt agtgtcccta ttattggatt gaaaaagaac
     1981 atcatgaaag acataaggaa tgccatggca acaccactgg tcatctgttc gatagccatc
     2041 attgagtaac cttccagatc cgctatggta gtggttaaaa ttgtttggat ggttccaatg
     2101 ttctgctctg aaaaataacc cataggtgct tgttttaagc gctcaccaat ttctaaacgg
     2161 taatcacgga aaatatcata accggttccg ctcatggtac ggtcactcat ccattggaaa
     2221 aagaaacgtc caagaatgct aataagaatg atagttacag cttttaaaat tattgaggat
     2281 gttagtgtat taagattcaa catgattaaa aatacactga gcatcataaa actttgaaaa
     2341 aaggatttta aaatattgca cagtattcca actgttattt tttttctgct ggatccggcg
     2401 atttgataaa tacgagaaat catttcaaac atctaagatt ctcctttcgt tttggaagtt
     2461 accgcatctg aataattctc ccacattttc ttataaagag ggcagttctc taacagttca
     2521 ttttgcgttc cgcatccagc tattttccca tcagctacaa caaggatttg atcggcattg
     2581 cggattgtat tcagacgatg tgcaacaaca attagggttt ttccggaaat taacttaccg
     2641 attgcttctt gtatcagtgc ttcattttcc gggtcagcat aggcagttgc ttcatcaaga
     2701 ataaccacgg aagctttttt aagcatggca cgcgctatgg tgatacgttg gcgttctcca
     2761 ccggataggc ggtctccggc atctccggcc atagtgtcgt agcctttttc taattgcatg
     2821 ataaaatcat ggcagtttgc agcttttgca acggtttcta tttgttcgtc cgttgcggtt
     2881 ggatttccca tgcggatatt atcacggatg gttttatcaa aaagaaagtt atcctgtgca
     2941 acataactga tttctcccat cagctgctca aaggggatgt tgcgaatatc ctgtgaacca
     3001 aaaagcacag tcccggaggt ggcatcccaa aaacccgcca ttaatttggc tatggtagat
     3061 ttaccagatc cggatgggcc gacaatcgct gtcattgatt tcggtgacgt cttaaatgag
     3121 atatcgtgca gcacttcact tttgttataa ccaaagcaaa cgtgactaaa ttcaaatgtc
     3181 ggttctgtta gagtaaccgg gtccacaggc cgctgcagtt caggagtttt taagaagaca
     3241 gttacttgtt caaggtttcc tttgatcata cttacctgct ccatggcttc cgatactttc
     3301 atcagcggcc ctgtaaagcc aagggggaca ataagacata tcatcagaat cggtaaagta
     3361 atcgaaccat tcataaaaag caaagcgcct ataggaagcg tgccaagtaa ggtggaggga
     3421 aggactgcac gagcggccgc attccatagc cagcactcac tccaccattc catggtgcag
     3481 tcgtggaagt aattgacagc ttttgagtat tgcccatagg acgagctgga ccggttaaaa
     3541 gctttaatga cctgaatacc atttacatat tccacaagag aactgttcat ttcattcgca
     3601 gaacacatat agttttccat acgggaggca tatccgcgca tcatggcagc gaaaaacaaa
     3661 cctcccaacg ggacagtaat taaagaagcc agccccatgc gccaatccaa aataaagatt
     3721 aatagaatac tgcatagagg tgctgtaatg tgggacggaa gttcaggcat aaaatgggcc
     3781 atagaatcct ctaactttgc cacattatct acaattaagt ttttaaaatt gccggaaggt
     3841 gtttccaaca tgacgcccat gggaaccttc gccattttat cggctatttt tttacgaata
     3901 ttttgtaaaa tagtaaatga aatacggtgg gacaaaaggg acgacctcca tgtaaggaag
     3961 gtcttaatgc actggcaaag tgccgcgatt ccagacaata tcaccgtatt tttagtagta
     4021 gctgttccgg aatagagtga atctgccaac atagccagca taaggaatgg aaccatacca
     4081 aatagttctc ctaaaacggc cataaaaatg gaaaatttca ttttcccttt gctactgcca
     4141 acaaacataa aaagttgccg gatagcccca ggttcttttg atttcatata aaatgtcctc
     4201 ctttttgcta atcagatttt gtaattgagt attgattttt taggaaaatg gtgctaaaat
     4261 tatattacga tgtaatatta cagtgtaata tagtatagaa tggaggtgag gaaatgtcaa
     4321 gggatttcca gcagactcat gaaaatctta ttgcctgtgc aaagaaacac ttcttagaat
     4381 ttggctttga aagagccagt attagggaga tctgtaaaga tgcaaatgta actaatggtg
     4441 ccttttacaa tcattttgct gataaggaag ctttatttgg ttctttggtg gaatcagttg
     4501 ttcagacaat ccaaaagata tattcagaat caatagataa gcattttgat ttagtaaaaa
     4561 cagatgaact taagaatttg tggaagctat cagaaagcac gatcatccag ataattgaat
     4621 atatttatga gaattttgat gtattcaggc tgcttctgat gtgctcgtca ggaacaaagt
     4681 atgcaggctt tttagacgac ttggtacgcg cagatgtgca ggagactata aaactcattg
     4741 cagaattaaa agcacgcggt gtacctgtca atgatttaga tgaggatgag tggcatatgt
     4801 tggttcattc ttattatgct tcaattgcag aaattgttat gcacaattat ccaaagccag
     4861 ctgcattgaa gtatactcat acgctatcgg tatttttcag ctcgggctgg caaactgttt
     4921 tggggattta atccccaaaa aatttcccat acagttagtt gaacctaact ataaaacatg
     4981 aaattaatca taacgaaagg acgattaaaa tgcaaaaatc taataaacta aatggaaaag
     5041 atcttatcaa tgtaggaatt tatactgcca tgactcttgt aatatttttt gttttcggat
     5101 tactgactgc actgcctata atatatccat tccttctgtt tatctggccg tttgtatgtg
     5161 gcattcctat gatgctgtac tacacaaaaa tccagaaatt cgggatgctg acaattaccg
     5221 gcgtcatctg tggtctgttt ttctttctta taggctatac atggattggg ctggtggggt
     5281 ggacactagg cggaattctt gctgatattg tattaaaggc tggtcagtat aaaagtttta
     5341 aagttactct cttaagctat gcgtgttttt gccttggtat gatgggatgc cctgcgaatc
     5401 tttggattgc cggccagtct tactgggaaa atattcatag ctccatgggg gatcaatacg
     5461 cagaagcatt acaatccatg atgccatcat ggatgctgta tgcaggattt gtgattttgt
     5521 ttgtcggtgg aatttgcggg gctgtgctcg gtcataagat gctaaagaag cactttgaaa
     5581 gggcaggtat tgtttgatgc acatgtttga tattacagag ataaaagggt tccatttaga
     5641 tccacgaaca aaattactgt tgatggccgt tgtagctact gccgaatttt tatacggcca
     5701 taccgctttt atgcttgcag tggctttgat accttttatt ctattactta caaataggca
     5761 gtataaggca gcgatcattt ttatagttct gtttgtggct gctttggtgg ttaaggaaat
     5821 ccaaaactca attcattttc atgtggttgt caatatggtt gtagttttat tggtgggatt
     5881 ggttttaaga ctttttcctg cttttgcaat gggaaattac ataattaaat ctactactgc
     5941 cagcgagtgt attacatcgc tgagcagaat gtatatcggt aggaacatta ccattcccct
     6001 ttctgtgcta tttcgttttt tgcctaccat gcaagaagag tcagcagcta ttaaagacgc
     6061 tatgagaatg agggaaatcc agtttggtac gaaaaaattt tggcaaaatc caatggctct
     6121 gcttgaatat cgtttcatac cgcttatgat ttcggttgta aagattggtg atgagctgtc
     6181 tgcagcagca ctaacccgtg ggttggataa tccagcaaag cggtcaagta taacgaaagt
     6241 tggatttacc tattatgatg ccattgcgtt ggtgatttca ggcattatgc tgtttattac
     6301 cttatttatt attaaatagt agaagaggaa ttgaaatgat agaaattaaa aatgtgtcct
     6361 ttacctatga aagtggggaa tcagagaata gcctgcgtaa tatcaatctg aaaattgaag
     6421 atggagagac tattttacta tgcggtgaat ctggttgcgg taaaacaact ttgacacgtc
     6481 tgattaatgg actgattccg cattattata atggacagtt aactggacag atttacttgg
     6541 atgggaaaga ggtaaaggat tatcagttat atcagattgc ctccatggtt ggctctgtat
     6601 ttcaaaatcc aagaacacag ttttacaatg tagatacgac aagcgagatt gtgtttggat
     6661 gtgaaaatat gggattgtcg gtgcaggaaa tgctggcaag gcttgagaat acaacaaaac
     6721 gccttaaatt agagaatctg cttggacgga gcctgtttgc tttatcgggt ggagaaaagc
     6781 agaaaatagc ttgtgcatcg gcagatgcta tctaccctaa tacttttgtt ttagatgaac
     6841 cgtcttctaa tttggatatt tcttcgataa aagatttaac agaagtaatt cgccaatggc
     6901 aatctgaaaa gaagacagta atcgttgccg aacaccgcct gtattacctg gctccatatg
     6961 cggaccgaat catatatatg aagcgcggag agatatgtaa tgaatataca agagaggaat
     7021 ttttggaact agatccaaag gagttaaaga aaatgggatt acgtgcatta aatccatttg
     7081 acttgatgcc tgaaaaaaaa cctgaagtaa atgaaaaaag cctgcagatt gataactttt
     7141 ggttttcata tgaaaaacgg ggatatccga ttgtaaacat accgaattta acattgccgc
     7201 agggagaaat cattggaatc attggtgaca atggagctgg taaatcctct tttgccagat
     7261 gtctatgcgg attagataaa tcttcaaagg gcactttgaa attaaatggc aattccctta
     7321 atgcgaagaa gcgccggcat atttcctata tggtaatgca ggatgtaaat caccagcttt
     7381 ttacagaaga tgtacttgat gaacttttgc tgagtatgga tggagaaaat gaggaagagg
     7441 acaaggttcg ggcacaagaa attttggctg ggcttgattt gtcggataaa ctgaaactgc
     7501 acccaatgtc gttatccggt ggtgaaaagc agcgagtcgc tattggcagt gctgttgcat
     7561 ctgggaaaga aataatcata tttgatgaac ccaccagcgg attggattat agacatatgc
     7621 ttgaggtttc agaaaaatta atacagctaa aagaaatgaa aaaaacgttg tttcttataa
     7681 cgcatgatcc tgaactgata tacaaatgct gtacacatct tatgtttatt gagcatggcc
     7741 gtatattatg gcatcggcct atggataacg aggctgtaag acttttacag gaatttttct
     7801 cccacgaaaa gggtgattca gcaatggcta tatgaaagcg aaagagtact ttgaagtggg
     7861 ctgagagaaa tataaggact aaagagaact tatttcggat atagaaatat gtaagagtaa
     7921 gctggaagtt aatataatga ttcaggccgc agtacttttt ataatagatg gcaatgtcga
     7981 tttatcaaaa agtctgcggc aattatttta taaatttgtt aaaaagatat tttgggagga
     8041 aacgtatgac cataaaaaat cggtattttc catttgtgtc tgaaaatgta gctgacagta
     8101 ataaggggac tatcctgttt tgctttcatc atgcaggagg aacagcaaca acatatcggc
     8161 cgtggacttt gaaatctaat gattcagtga ttattatgtg tgtcgagctg cctggaaagg
     8221 gaaccaggcg gactgaaaga atggctgttg atttcaatga gattctgccg gaactgtcat
     8281 tgcaaattgc tgatgttagc tgtggcagaa agattgttct gtatgggcac agtatggggg
     8341 cagcaatggc gttttatacg gcacattatt tatggaatca attagacaga aaatgtgaaa
     8401 aagtaattgt tgccgggcgt cagcctccgg atcaggaaaa tccctatgaa tttaagtcct
     8461 atatgaatga cgatgcttta attgaggagt tagttcggta taatgccaca ccaaaggaag
     8521 tgctagaaaa taaagagctg ctgaatttta ttcttccggg tctgcgtcag gattatatat
     8581 taaatgaaag tctggtttat catggagaag tgttggatat accgatagta gcgcataccg
     8641 gaagccagga ttacgaggca aatgcagaga taatgaattt gtggaaacat atgacaacca
     8701 atatctttca gttgaatttg ttcgagggtt cccatttctt tgttacggat ttaggcaata
     8761 agtataggga tattgtgata caggaagcaa ttaataaacg ggaggattaa gcctatgaag
     8821 gagaacattt cgattataaa aggaatggac acgttaagaa caattgcaga ggaacggaag
     8881 gtatcggtta atgtactgct aaagggaatc gcagtggaaa tatattatcg ttggtttgca
     8941 aatgaaacag caatagaata tgtgacgctg aatgatttga ttagtgaggt aatcgacagg
     9001 tccaaggata aagataccat agttatcaat gattacgtgt gcagtgtaat gtgcaacaat
     9061 gataaggctg ttctgtgcat tgagtgtgag cagttggaat gtgatatgat agaaggcttg
     9121 agcgctttta ctgaggaatt tgttttggag tgggcagatt tagctgataa tgctccaatt
     9181 gtccaccaaa ttccgagaaa tcagatggct gtcaggaaga atagaaatag gacgaaaaaa
     9241 acattttcag atgaatgcct acatcatgga ttttttgaaa atgcggcaaa tgcgcctggg
     9301 aaagcagcgc ttatatatta caagaatgga aataaagaag aaattagtta tttctgctta
     9361 caggatatga ttttaaagtt agctggatat ttaaaggaaa atggtgtaaa aagcggaacg
     9421 ctggtcggtg taagtatacc caaaggcatt aatcaggtta ttgccgttta tggtattttg
     9481 gctgcaggag caacatatgt gcctattggt gttcaccagc ctgtagaaag aaaaaagaaa
     9541 attatagata ctggaagaat aaggtatatc gtaacaagca atgaaatggg gtcagaagaa
     9601 gatttaggag tatctgtgat ctgccttgaa gcggttcttc agaaagcgtt accggcaaaa
     9661 gtacctgtct ttcccgaaac gaaccaaccg gcttatatta tttttacatc gggtacaact
     9721 ggtataccta aaggtgtggt tatctcccat agtgaggccc ataatacaat agctgatata
     9781 aatcaacgat ttaatatagg caggaatcat actgggatcg ctatttcaga cttggatttt
     9841 gatttatctg tttatgatat ttttggattg ttatcagctg ggggaacctt ggtggtattg
     9901 agtgaggaaa caaaaaggga gcctgtcttt tggagaaaag caattattga tgcaggcgta
     9961 aatgtttgga actcggttcc ggcattgttt gatatgttat taaccacctg tgaaactgat
    10021 aaacaaatgc tgccgcttca actggtttta ttatccggag attggattaa gatggattta
    10081 tatgacaggc tgaaagcaat cagtgaatgc tgtaagtttg tttctctggg aggagcaacg
    10141 gaagcggcca tatggtctaa ttattttgtt gtgaacgata ttgacagcgg ctggaagtca
    10201 ataccatatg gtgaacccct gagcaatcag tatctgagag ttgttgatgg taatggatat
    10261 gattgtccgg attatgtaaa tggggaactg tggattggcg gagacggtgt agcagaaggc
    10321 tacttaaatg accctgaatt aacagatgcg aagtttgttg caatagatgg ggtaaggtgg
    10381 tataaaaccg gcgatttggt ccggtataat tctgcaggga ttgtagaatt tctgggtaga
    10441 atcgacaatc aggttaagat taatggctac cgaatcgaat tgggagaagt agaaaatgtt
    10501 attaagcgtt ctccggacgt ttctaatgta gtggtaggtg tggttgatga gaaaggaaaa
    10561 aaggaattag gagctgtgat tgttccgaag ataaatgctg ctgtcaatgt agatattact
    10621 tgttgtgagg ataacaacaa gtattcggat ttccatatga aggaacgtga aagaattgtc
    10681 aggcagttga ttcttgaatt ctgtcatatg caatcaaggg tatcagcaga gtataggccg
    10741 attatggaat tttgggaggg ctggcttgaa tcccatatgg aatattcagc agatatattt
    10801 tccgccagag aggaaatgga accattgcaa aaggttatga atgcaaggaa tctttttgcg
    10861 gagatactca cgggtgagca acgtgtggag gagctgcttc ggagggagga ggtttcacca
    10921 gagttctggt ctttaaaggg agaagatacg aagtactttt tagacaagat actggcaggc
    10981 gatataagta acaggaaaat cgcagttttg ggtgcaagaa caggagaaat tataaagcaa
    11041 tactgggacg gattctgcca ggcagaggag ataacgttat ttgatacttc agcgggaata
    11101 ttgaagttgg cacaggaaaa actgggagga atgaatgcaa atatccgcta ttgcagtaca
    11161 tacaatgagg gtgtggaaga atcggaactg aataaatatg atattgtttt ggcggtaaat
    11221 ctattgcata cctatgagga acctttaaag gaagtgaagt gggcagcgct tctgctgaag
    11281 aggaaagggg acttttatgc aatcgagtat gaggagttag accctatggg tattcttatt
    11341 tctggattgc tggagaatgg atttgttaac agaaagcggg gaagacgaga gcatacgcct
    11401 ttactattat tggatgattg gaaaaatatt tatggacagg ctccttttgg tgaggttgca
    11461 ataaacagaa gaggaagatt gggagctctg ttaatccacg ctaaaaacgt aacaccagaa
    11521 gtaatagaaa tgagatctcg tatattagaa tacatgaatg aaaatctgac agcctacatg
    11581 attccgacta aacgcatgat tgtaaagcaa gtagtattga gcaaaaatgg aaaagtgaat
    11641 agacagcagt cattagcgtt attggctgta aaacaaacag cggaaaagga gagtatcata
    11701 cttcagggaa ttgaagctga tattgcagag ctttggacaa acattctgag ttgcaaaata
    11761 tttgatagga gccagagctt ttttgaatcg ggcggagata gtctatcagc aactcggttc
    11821 ctggcggaaa tcaagaagaa atactctgtg gatataccat taaaggatat ctttagcacg
    11881 ccatctctca agtctgttgc ggatatgctt caggccaggc ttacggagaa tgaaggtatg
    11941 gttgaaggcg agatataaaa ctctggaagg agcgttgact ctatggataa aacaaaagag
    12001 ttcatatggc tggcagataa tgagttgaga ataaatttaa ataaattagc cagtgatgga
    12061 atcaatgtat ggttagagga tgaaaagcta aagtttaaag gcgaacaggg caaggttacg
    12121 gaagaggtat taaaatggat gcggcagaat aaaacagcaa ttatatccta tctcttggag
    12181 aaggaggagg ttcaggataa gggatttgat ttaaccccga tacaaaaggc gtatttcttg
    12241 ggtagggata acctctatga attgggaggt atcagtgcta attactattt tgaaatagat
    12301 atggataagc tggatgttaa gaaatttgaa tcaatattta accaggtgat tcatgataat
    12361 gaggcactga gaggcattat cttgaagtgt gggaggcagg ctgttttaag taacgtaccg
    12421 tggttctcca ttgaagtgaa aagagtaaat gatgaagcag agcaaaaagc gctgcggtcg
    12481 gagtgggaaa gcaaaatata tccgttggag tcttggccta tgtttcatgt gtgtctgaca
    12541 acaaaggata attgctgtta caggctgcat gtggggtttg actgtatttt gctggatgcc
    12601 tggagtgtca cacttatgct gaagcagata tataaacttt atgaaaatga taagattatg
    12661 gttcctgcat acacctttag agaatattgc aatgaacatg ggaagcttct gaatgaaaaa
    12721 cttaaggagc aggctgagcg gtattggagc cagcggtgtg aaatgcttcc gaaggcaccg
    12781 gccttgccat atgcaaaaaa actatcggaa attaatatac cgcattttag aagattgaat
    12841 tatgagttac cgaaattcga tacagaaaca atatatatga aggcaaagga atacaaagta
    12901 accccggcag cagtcctgtg tac
//
