LOCUS       c00298_gnlPro..         3094 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NIGDIANH_298.
ACCESSION   c00298_gnlPro..
VERSION     c00298_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 03:53:29
            Original ID  :: gnl|Prokka|NIGDIANH_298
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3094
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3094
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[715:3092]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      716..3092
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3094
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3094
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..398
                     /locus_tag="ctg298_1"
                     /transl_table=11
                     /translation="CGLGASVGCERIKRTFNTPRLKSQICLIRGNGFVCSSLNELNSNP
                     LTFCSILFIVRILIRLTEVSGMNHMKYKTLIADRLGNSCLDAGFFISDKIIFYPISLIR
                     SNFKSLLSHVLKKMFFQTCSKERKIKL"
     CDS             716..2968
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg298_2"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.7e-268, bitscore:
                     877.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQKEQWQGFNGRIWREEINLREFIQDNYTPYDGDASFLEGPTEA
                     TDTLWGELQKLQKAEREKGGVLDMDTDIVSSLTSHKPGYISESLKEKEQIVGLQTDKPL
                     KRAFMPFGGIRTAEEACTTYGYTPNPEFHKIFTDYHKTHNQAVFDSYTPEMKKARHNKI
                     ITGLPDTYGRGRIVGDYRRVALYGIDLLVADKQKDFANCGDGTMTDDVIRLREEISMQI
                     KALKDMKVMAESYGYDISEPASNAKEAVQWTYFGYLAAIKTQNGAAMSIGRIATFLDIY
                     IKRDMDKGILTEKEAQELIDHMTMKFRMVKFGRIPAYNQLFSGDPVWATLEVGGLGQDG
                     RSMVTKTDYRFLHTLENMGPSPEPNLTVLYSERLPKAFRDYAAHISITTSSIQYENDDV
                     MRPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKTGQQVGPEYKP
                     ITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYEYALMALIDTNVRRTFA
                     TGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKYGNDDDRADDIAVWLLQ
                     TFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQ
                     NGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERIGNLVNVMDGYFAQGAHHL
                     NVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQQLDVIARTCHDRM"
     CDS             2955..3092
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg298_3"
                     /sec_met_domain="Fer4_12 (E-value: 3.3e-11, bitscore: 31.4,
                     seeds: 82, tool: rule-based-clusters)"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-07, bitscore:
                     20.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     D"
ORIGIN
        1 tttgtggtct cggagcaagt gttggatgcg agcgcataaa gcgaacattc aacacaccgc
       61 gactaaaatc acaaatatgc cttattcggg gcaatggctt cgtctgtagt tcgcttaatg
      121 aattaaatag taaccccttg acattttgtt ctattttgtt tatagttaga atacttatac
      181 gactgacgga agtaagtgga atgaaccaca tgaagtataa gactcttata gccgaccgtc
      241 tgggcaattc ttgtctggat gctggctttt ttatttctga taagataata ttctatccaa
      301 tatccctgat aagaagtaac tttaaatcgt tattaagtca cgtgctaaag aagatgtttt
      361 tccaaacatg ctctaaagag cgaaaaataa aattatagtt gtatgcttgt cattattgta
      421 cttatatcag tacatttgta cgatttgata atattttatc aacgctctaa aaccctttat
      481 tttaaaggct ctacaagcat ttttgtgtat atgttcaaaa aaaagaacac ataatatcaa
      541 aaaattgttt gacgattgcc aaaaatcatg ttaataatgt tgttagatga ttaaaactta
      601 aataagttaa aactaatcat agtaaatcat ctgaacaatt ttgttgattt tttagcaaac
      661 atatttcaaa accttgactt aaggattttt ttaacaaaca gggaggtcaa ttaaaatgtt
      721 acaaaaagaa caatggcagg gatttaatgg acgaatctgg agagaagaaa ttaatttaag
      781 agagttcatc caggataact acactccata tgatggagat gcttctttcc tcgaaggccc
      841 taccgaggct actgacacac tgtggggcga actgcagaag ctgcagaaag cagagcgtga
      901 aaagggtggt gttcttgata tggatactga tatcgtatca agccttactt cccataaacc
      961 gggttacatt tctgagtctt taaaagagaa agaacagatc gtaggtcttc agacagataa
     1021 acctttaaaa cgtgcattta tgccatttgg tggtatccgt actgctgaag aagcttgtac
     1081 aacttatggt tacacaccaa atccagaatt ccacaagatt tttacagatt atcataagac
     1141 acataaccag gcagtatttg attcttatac tcctgaaatg aagaaagctc gtcacaataa
     1201 gatcattact ggtcttccag atacttacgg acgtggacgt atcgttggtg actaccgtcg
     1261 tgtagctctt tacggtattg acctgttagt tgctgacaag caaaaagatt tcgcaaactg
     1321 cggtgacgga acaatgacag atgacgttat ccgtttaaga gaagaaatct ccatgcagat
     1381 taaagcatta aaagatatga aagttatggc tgaaagctat ggctatgata tttccgaacc
     1441 tgcttctaac gcaaaggaag ctgttcagtg gacatacttt ggataccttg cagcaatcaa
     1501 gacacagaac ggtgctgcta tgagtatcgg acgtatcgct actttccttg acatttatat
     1561 caaacgtgat atggataagg gaatccttac agagaaagaa gcgcaggaat taatcgacca
     1621 tatgactatg aagttccgta tggttaagtt cggacgtatc ccagcttaca accagctctt
     1681 ctccggtgac cctgtatggg caacacttga agttggcgga cttggacagg acggtcgttc
     1741 catggttaca aaaactgact accgtttcct ccacacatta gaaaatatgg gaccatcccc
     1801 agagccaaac ctcacagtac tttactctga gcgtcttcca aaagcattta gagattacgc
     1861 agctcatatt tctattacaa caagttcaat ccagtatgaa aatgatgatg taatgcgtcc
     1921 agtatggggc gatgattact ccatctgctg ctgtgtttct gctacacaga ctggtaagga
     1981 aatgcagttc ttcggagccc gtgcaaacct tgcaaaatgt ctcctctacg caatcaatgg
     2041 tggtgtagat gaaaagactg gtcagcaagt tggtccggaa tacaaaccaa ttacttccga
     2101 atatcttgac tatgatgaag taatgcacaa atatgatatc atgatggact ggttagcagg
     2161 tttatatgta aatacattaa atctcatcca gtacatgcat gacaaatatt actatgaata
     2221 tgctttgatg gccttaatcg atacaaatgt acgtcgtaca ttcgctacag gtatcgcagg
     2281 attctcacac gtagtagact ccttaagtgc tatcaaatat gcaaaggtta agacagttcg
     2341 taacgaagaa ggactcgttg tagattatga aacaactggc gacttcccta aatacggaaa
     2401 tgatgacgac cgcgccgatg atatcgctgt atggttactt cagacattta tgaagaaact
     2461 ggagaagttc catacttatc gtgattctga acctacaact tctatcctta ctatcacatc
     2521 taacgtagta tatggtaagg caacaggttc ccttccagat ggaagaaaag ccggtgaacc
     2581 actttcccca ggagcaaacc caagttatgg tgcagagcag aacggtctct tagcttcctt
     2641 aaactctgta gctaaacttc cttatgaatg ggcattagat ggtatttcca atacgcagac
     2701 aatcaaccct gatgcactcg gtcattccga agaagaacgt attggtaact tagtaaatgt
     2761 tatggacggc tacttcgcac agggtgctca ccacctaaac gtaaatgtat ttggtgttga
     2821 gaaactgatt gatgctatgg aacatccgga aaaagaagaa tacgcaaact ttacgatccg
     2881 tgtttcaggt tatgcagtta aattcatctc tctgacaaaa gaacagcagt tagatgtaat
     2941 tgccagaaca tgccatgaca gaatgtaata aaaatgcgat ggctcttgtt cattctacgg
     3001 agagttttgg ttcggtggat gggccgggag ttcgttttat tgttttttta cagggttgtc
     3061 ctttgcgatg tcagttctgt cacaatccag atac
//
