LOCUS       c00027_gnlPro..        15830 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MLCGPKPE_27.
ACCESSION   c00027_gnlPro..
VERSION     c00027_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 23:57:13
            Original ID  :: gnl|Prokka|MLCGPKPE_27
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15830
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..906
                     /locus_tag="ctg27_1"
                     /transl_table=11
                     /translation="ANASSSYRKESDSQKGKSSFAKITEKFTEKKEDIILACDDLWFRY
                     DKELPDVVKGLSFSLKRGEFFALLGGNGTGKSTTLSLLSRLRKPYRGKVLLEGKDIRRY
                     SDKELFRNFLGVLPQNPQSLFVKKSVELELFEMVGGVKEKKDEEYQLAMDKKDAVEGII
                     KVTHLEELLHRHPYDLSGGEQQRLALAKILLLRPKLLLMDEPTKGLDSYFKKEFAGILA
                     LLKEQGVTVFMISHDIEFCASYADRCGLFFDGNIVACGTPGEFFAGNNFYTTAANRMAR
                     RYFPMAVTGEDVVECLKKGV"
     protocluster    1..15830
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4531:9504]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4532..9504
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..15830
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15830
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             910..1884
                     /locus_tag="ctg27_2"
                     /transl_table=11
                     /translation="MADWKSSQEFLKEFYASPIVNEDTISAKIKRIFLPKSRKASALKE
                     LAGDLSGEGRAGDVILDNDMFEMDTEIWKKQEKRRLSVRTRVSLFLLLVAIPLTIAVGF
                     YVMDVAAVGTVFEKIFGPRKYYFISLVIIIYSLLPFFMVFEGRKPQVRELVVLATMVAL
                     ATAGRAAFFMIPSFKPIIAIVIISAIAFGGEAGFLVGAMTMVVSNFLFGQGPWTPWQMF
                     AMGFIGFLSGILYRIGLLPTKRLSLCIYGFLVTVFIYGGIMNPAALFMSVYDFTWEGLL
                     AIYISGIPVDLVHASSTFIFLWIGAKPLLEKLQRIKIKYDLMR"
     CDS             2021..3034
                     /locus_tag="ctg27_3"
                     /transl_table=11
                     /translation="MQKIPECRSMNFSIYEGMLNRYKDSEDIRKSYESRGLNGLEVIRA
                     GEPDEGKILPDMVNGVHLYFHIFWMDWWKGNYERLDQEFDSREQWLEYFGGMSRESYLN
                     SLRDDLAYAEQVGAKYVVFHVSEVTLRESYIYKYRYTDKEVIDASLEVINTLLDEKEYP
                     FDFLVENLWWSGLTLKNVRLTRRLIEGMHTQKKGIMLDTGHYMNTTTQLKTPEDAVAYL
                     NKMIDKYEKAQMLHWFKGMHLQLSLGGDYVRKQRKEWREHPIDFDKIPFYELFRLAYDH
                     ACHIDLHQPFIGEGVREFVERVAPKYVTLEYQQNSREEYEQFVETQSKILKWITIH"
     CDS             complement(3168..3809)
                     /locus_tag="ctg27_4"
                     /transl_table=11
                     /translation="MENRKIQFRSKACNLHLSAYPGHFATKHSHVNYFLDMTTLKVRQS
                     NAEEAARALVPLYKHNTVVDTIVCLDGTEVIGAFLAEKLTESGFFSYNQHKSIYIVTPE
                     IDSDGQMFFRKNIQPMIKDRNIIILMASVTTGITINRSWECIEYYGGKLQGISAIFSTL
                     SEYGNLPVNALFTPNDIPTYHTYDKHDCPYCKSGQKIDALVNGHGYSELL"
     CDS             4532..5956
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_5"
                     /sec_met_domain="CT_Vparvula (E-value: 1.1e-137, bitscore:
                     449.5, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGNSKMNARERIDYLFDDHSFVEVGGYVSARNTDYNLGAKKVEGD
                     GVITGYGVINDRMVYVYSQDASALGGSVGEMHAKKIASLYDLAMKTGAPVIGMLDCAGL
                     RLQEATDALHAFGTIFNKQAQASGMIPQISAVFGMCGGGSAVMSAMSDFTLMEKENGSL
                     FINSPNALAGNEESRQNTAGAQFQSEQAGNVDFVCETEEGLLDQLRILVDVLPSDYEAE
                     DVYFDNDDDLNRVIPELNDAAYDAAYILKSISDNNMYIETKAAYAKEMVTAFIRLNGET
                     VGAVANQPVDGESVLTRNGLEKAERFIKFCDAFSIPLLTVTNVTGLRANAHEERKQALA
                     LAKFTTTLAQVSVPRINLIAGEAYGTAAEVMNSKAIGADFVLAWPQASVGMMDAEQAVR
                     IMYAEEINTDKNKTALIAEKTAEYAEGQSSALAAAKRGYIDDIIEPDATRKRLIAAYEM
                     LYGKKVTPVTKKHTAV"
     CDS             5969..6313
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_6"
                     /sec_met_domain="OAD_gamma (E-value: 6.8e-28, bitscore:
                     87.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVFEQALLNTLMGMGTVFAVLIFISLLISLFVYIPSIERALKNR
                     SSKKEKKAAQEERPAPKRPILEEVVEEEELVDDGELVAVITAAIMAANGGAAVSADKLV
                     VRSIKRVKRR"
     CDS             6408..6776
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 66.6; E-value:
                     4.3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_7"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1e-21, bitscore:
                     66.6, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNYRITVNGVAYDVAVEELGAGEASAAPVSTPAAVPAPAKKAAP
                     KTSGSAGAVKITAPMPGKIVAVKAQAGASVKKGDAVLVLEAMKMENEICAAQDGVIASV
                     EVAVGDMVEGGDVLATMN"
     CDS             6810..7934
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_8"
                     /sec_met_domain="OAD_beta (E-value: 1.4e-157, bitscore:
                     514.1, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYVVETMTNLAAQTGFAFLKPGNFIMILVALVFLYLAIAKGYEP
                     LLLVPISFGMLLVNLYPSIMEEGGLLHYFYLLDEWSILPSLIFLGVGALTDFGPLIANP
                     ASFLLGAAAQFGIYSAYFLAMLMGFNGKAAAAISIIGGADGPTSIFLAGKLGQTDLMGP
                     IAVAAYSYMSLVPIIQPPIMKLLTTEKERQIKMEQLRTVTKTEKIMFPIVVTVVVCLIL
                     PTTAPLVGMLMLGNLFRESGVVKQLSETASNALMYIVVIILGTSVGATTSAEAFLNVST
                     IKIVVLGLVAFAVGTAAGVLFGKLMCKVTGGKVNPLIGSAGVSAVPMAARVSQKVGAEA
                     DPSNFLLMHAMGPNVAGVIGTAVAAGTFMAIFGV"
     CDS             8092..9504
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: PYC_OADA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: HMGL-like"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_9"
                     /sec_met_domain="HMGL-like (E-value: 9e-44, bitscore:
                     139.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="PYC_OADA (E-value: 4.6e-59, bitscore:
                     189.5, seeds: 434, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEIKKKPVKIVETVLRDAHQSLIATRMTTEEMLPIVDKMDQVGY
                     HAVECWGGATFDACLRFLKEDPWERLRKLRDGFKNTKLQMLFRGQNILGYKPYPDDVVE
                     YFVQKSIANGIDVIRIFDCLNDLRNLQTAVDATKKEGGHAQIALSYTIGDAYTLDYWKK
                     TAKNIEEMGADSICIKDMAGLLVPNEATRLVTALKQATDIPIELHTHYTSGVGGMTYLK
                     AVEAGVDIIDTAISPFAMGTSQPATEVMAETFRGTEFDTGFDQKLLGEIADYFRPIREK
                     YIANGLMSPKVLGVNIKTLMYQVPGGMLSNLVSQLEQQGASDKFYEVLEEVPKVRKDFG
                     EPPLVTPSSQIVGTQAVLNVLTGERYKMITNESKALLRGEYGQTVKPMNKEVQKKAIGD
                     EKPITCRPADLLEPELSKIEAEMSQWKEQDEDVLTYALFPQVATEFFKYRAAQENKIDP
                     AKADVKNGAYPV"
     CDS             complement(9765..10154)
                     /locus_tag="ctg27_10"
                     /transl_table=11
                     /translation="MKKLFYPAVFHIAEEGGYWVTFPDFPECLTQGENMQEAYDMAIDA
                     LGLILTDDINDHKELPEPSNITHVDDGVVVIIPYDYLEYNRKYHNKSIKKTLTIPEWLN
                     NEALRLNLNFSQILQDALLNKIQSR"
     CDS             complement(10200..10388)
                     /locus_tag="ctg27_11"
                     /transl_table=11
                     /translation="MPMKPKEMIRLLKKNGFIKISQNGSHVKMKNFKTGKQTTVPLHSK
                     DLKKRLEDAILKQAGLK"
     CDS             complement(10712..11740)
                     /locus_tag="ctg27_12"
                     /transl_table=11
                     /translation="MIPIQNIYYMLSYAFQTLQAENYKDLATENFHNTAELCAAILDKG
                     IGIQLKHGLRRDYVSKSESLSTLQGKLNISESIKTQTMLKKQMICTYDEFSTNIQFNQI
                     IKSTVLLLLKANITNSRKKSLRKLLLFFSDVNEIDLHFVNWNQQYNRSNQNYQMLIGMC
                     YLVYKGLLTTQNNGTTKLMDFFDGQRMCRLYEKFLLEYYRKEHPELTANASQIAWQLDD
                     TENQMLPRMQTDIMLSKNNNILIIDAKYYSHMTQQQYGIHTLHSNNLYQIFTYVKNKEF
                     ELRNYEHTVSGMLLYAQTDEDIIPNNTYHMSGNQISVLALDLNQDFSKISRTLDDIAKN
                     FL"
     CDS             complement(11737..13641)
                     /locus_tag="ctg27_13"
                     /transl_table=11
                     /translation="LNLCYVLEMDKDMREIFGSIAGGAAYKFGLFFHKKTQSWTCGSPA
                     KPVKLTENEAIQKAEEIRNDLVEGAEIISSFGPLNSTADYEQLYKQLEHISGINTVWRM
                     KYYQMLFPILFAPFYGQDIQLDVLHFLNQTPSEIPFIRMGQIALFSKKCNIPGIVFGHI
                     WGRSTNHNNKSNDSETNTLSDKKHKLHYWMYTVFDDTSWMECQQKEIMVLGMNDIGDYS
                     QYDSKESLRQELISTYDNSTSRKNQALMAWNFANKLAINDVIFAKRSNTLVGKGIVTGD
                     YIFDDSRQEYKNIRTVKWLQIGEWEHPGKSVAKRLTDITSYTDYIEKLITIFTPDELDD
                     VDTQPEVDYPEYSSADFLSDVYMSEQDYETLVNVLKMKKNIILQGAPGVGKTFTAKRLA
                     YSIIGAKNPDRVQMIQFHQSYSYEDFIEGYRPTENGFTIKKGSFYKFCKLAEDDDENDY
                     FFIIDEINRGNLSKIFGELFMLIEKDKRGIELQLLYSDENFSVPPNVYIIGMMNTADRS
                     LAMLDYALRRRFSFFTMKPGFNTIGFQTYQDSLKSDAFKKLISCIKQLNSKIAADISLG
                     EGFCIGHSYFCGLTAKTATVRTLTSIIEYELIPLLKEYWFDEPEKIIDWSDRLRSTVK"
     CDS             13818..14966
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg27_14"
                     /sec_met_domain="Aminotran_5 (E-value: 6.9e-79, bitscore:
                     255.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEIYFDNGATTRALPQVREIMDEVLEKEYGNPSSMHMKGFDAEK
                     YVNHAKEIIAKSLKVDPKEIYFTSGGTEANNLALIGTAFANKRERKHIITSCIEHASVY
                     NPLSFLEDEGFEVTYLPVDEHGIVDLEALKKALRKDTLMVSIMCVNNEIGAIEPVEEIG
                     KIVKSFDPKILFHTDCIQAYGKLNCYPKKWKADMISVSGHKIHGPKGIGFIYIKNGTKI
                     KPIIWGGNQQKGMRSGTENVPGIAGLGKAAELIYENHEEKIAHIREIKDHFIKRVMEEI
                     PDVKDNSGDAPHVASISFLGVRSEVLLHALEEREIYVSAGSACSSNKPEVSGTLKGIGL
                     TKEYYESTLRFSFSIFNTVEEADICVEALKELLPMLRRFTRR"
     CDS             15063..15830
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039:Thiamine biosynthesis protein (Score: 73.3;
                     E-value: 6.4e-23)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg27_15"
                     /sec_met_domain="ThiI (E-value: 1.5e-23, bitscore: 73.3,
                     seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFKAFLIKYAEIGIKGNNRRFFEDALVRQMKYALKPVGDFSIRK
                     ESGRIFVHANEEYDFDEAVESLSHVFGIAGICPIVVEEDKDFEKIAEVVEKYVDEDYAD
                     KHFSFKVHVRRADKKYPIPSMEAAARLGERLLEKFEDLSVDVHNPDVMLTVEIRDKVYI
                     YSREIPGPGGMPVGTNGRAMLLLSGGIDSPVAGYMISKRGVSIDATYFHAPPYTSERAK
                     QKVVDLAKEIAKYTGPITLHVVNFTDIQMAIYEK"
ORIGIN
        1 gcaaatgcat cttcttcata tagaaaagag agtgattccc aaaaaggaaa atcttctttt
       61 gctaagatta cagaaaagtt tacagagaaa aaagaagata tcatcttagc gtgtgatgat
      121 ctgtggttcc gctatgataa agaactgccg gatgtcgtaa agggactaag cttttcctta
      181 aaaagaggag aattctttgc attgcttgga ggaaatggaa ccgggaagtc aacaacactt
      241 tcacttttga gccgtttgag aaagccatat cgtggaaaag tacttttaga aggtaaggat
      301 atccgtcgtt acagcgataa agaactgttc cgcaattttc ttggagtact gccgcaaaac
      361 ccgcagtccc tttttgtaaa gaaaagtgtg gaattagaac tgtttgaaat ggtaggcggt
      421 gtaaaagaga aaaaggatga agaatatcag cttgccatgg ataaaaagga tgcggtggaa
      481 ggaatcatta aagtaacaca tttggaagaa ttactgcatc gtcatccgta tgacttaagc
      541 gggggagagc agcagaggct tgccctggct aagattttgt tattgcggcc aaagcttttg
      601 ttgatggatg aaccgaccaa aggacttgac agctatttta aaaaagaatt tgccggaata
      661 ctggcattac tcaaagagca gggcgtaact gtttttatga tttctcatga tattgagttt
      721 tgcgcttcgt atgcagaccg atgtggttta ttttttgatg gaaatattgt ggcatgtgga
      781 actccgggag aattctttgc cggaaataat ttttatacaa cagcggcaaa ccgaatggct
      841 agaaggtatt ttcccatggc ggttaccggg gaggacgttg tggaatgtct taaaaaagga
      901 gtgtgaacaa tggcagactg gaaatcaagt caggagttct tgaaagaatt ttacgcttct
      961 ccaatcgtga atgaagatac gatttctgca aagataaagc gtatcttttt accaaaaagc
     1021 aggaaagctt ctgctttaaa agaattagca ggagatttgt caggagaagg cagggcggga
     1081 gatgtcattt tagataatga tatgtttgag atggataccg aaatctggaa aaagcaggaa
     1141 aaacgcaggc tttctgttcg taccagagta agtctctttc tcttgcttgt agcgattccc
     1201 ctcacaattg ctgtcggatt ttatgttatg gatgttgccg ccgttggcac agtatttgaa
     1261 aagatttttg gtccaagaaa gtattatttt attagcctgg taatcattat atactcgtta
     1321 ttaccgtttt ttatggtatt tgagggacga aaacctcagg tgagagagct agttgttctt
     1381 gcaacaatgg tagcattggc aaccgccggc cgggcagcat tttttatgat accgagcttt
     1441 aagccgatta tagcaatcgt gatcatcagt gcaatcgcat ttggcggcga ggcaggattc
     1501 ttagttggtg ctatgacgat ggtagtcagc aatttcttat ttgggcaggg accatggacc
     1561 ccatggcaga tgtttgcgat gggttttatc ggatttttgt caggaatcct ttaccgaata
     1621 ggactgcttc cgaccaaacg gctcagcctt tgtatatatg gattccttgt gacggtgttc
     1681 atttatggag gaatcatgaa tccagcggct ctctttatga gtgtgtatga ttttacatgg
     1741 gaaggactgc ttgcaatcta tatttcaggt attccggtag atcttgttca tgccagttct
     1801 acatttattt ttctgtggat tggagcaaag ccattgttgg aaaagctgca gcggataaaa
     1861 ataaaatatg atctgatgag atgaatagcc ggggcgtttt tgaactttag aaagattgcc
     1921 agaatgatgg ggcatttttg aaataagtat ggatgataag tatagataaa atgtattctt
     1981 agaaagattc tgagagtata cgagattgaa gggggaaact atgcagaaaa taccagaatg
     2041 tcgttccatg aatttttcaa tttatgaagg gatgctaaat cgttataaag atagtgaaga
     2101 tattagaaag tcttatgagt cacgaggatt aaatggactg gaagtgattc gggcaggcga
     2161 gccagatgaa ggaaagattc tgccggatat ggtgaatggg gtacatctgt attttcatat
     2221 tttctggatg gactggtgga aggggaatta tgaacgccta gatcaggaat ttgattcaag
     2281 agagcagtgg ctggaatact ttggaggaat gagcagggaa tcttatctga acagtttgcg
     2341 ggacgatctt gcttatgcag aacaagttgg agcaaagtat gtagttttcc atgtttcaga
     2401 agtgacactc cgggagagct atatttataa ataccgctat acagataaag aagtgattga
     2461 tgcaagctta gaggtcatca ataccctact tgatgaaaaa gaatatccat tcgactttct
     2521 tgtagagaat ctctggtgga gcgggcttac cttgaaaaat gtccgcctta cccgccggct
     2581 tatagaagga atgcatacac aaaaaaaggg aatcatgctc gataccgggc attatatgaa
     2641 tacaacaaca cagctaaaga ctccggaaga tgccgtagca tatctgaata aaatgataga
     2701 caaatacgaa aaagcacaga tgctccactg gttcaaagga atgcacctgc agttgtcact
     2761 gggaggggat tatgtaagaa agcagcgaaa agaatggaga gaacatccga tagactttga
     2821 taagattcca ttttatgaac tatttcgcct tgcatatgac catgcctgtc atattgatct
     2881 gcatcagcct tttatcggag aaggtgtccg ggaatttgta gagcgcgtag caccaaagta
     2941 tgtcacactg gaatatcagc aaaacagccg ggaagaatat gaacaattcg tagaaaccca
     3001 aagtaaaatt ttgaaatgga ttacgattca ttaagagaaa acagacgaaa aaagaaacca
     3061 taccatatcc catctgctct gtagtcgttg tgtttaaaat gctcatccgc atctgggata
     3121 tggtatggtt tctttttttc gtctatacga aatctacaaa ttggtagtta tagtaattcg
     3181 gaatatccat gtccgtttac caaagcatcg attttctgac cactcttaca atatgggcag
     3241 tcatgcttat catatgtatg gtaagtagga atatcatttg gagtaaacag cgcattaaca
     3301 ggaagattac catattcaga aagcgtacta aatattgctg aaattccctg cagtttgcca
     3361 ccatagtact cgatacattc ccagcttcgg ttgattgtga ttccagttgt gacagatgcc
     3421 attaaaataa tgatatttct atctttaatc attggctgga tgtttttgcg gaagaacatc
     3481 tgaccatctg aatctatttc tggagtaaca atatagatag atttatgctg gttgtaagag
     3541 aagaatccgc tctctgtaag cttttctgct aaaaatgcac cgattacttc tgttccgtca
     3601 agacatacga tggtatcgac gacggtatta tgtttataaa gtggaactaa cgctcttgca
     3661 gcttcttctg cattagactg acgtactttt aatgttgtca tatctaaaaa gtagttgaca
     3721 tgagaatgct ttgttgcgaa atgtcccgga taggcagaaa gatgtagatt acaggctttt
     3781 gaacgaaatt gaattttgcg gttttccata agatgtcctc ctcctttatt gtgaaagaat
     3841 tgtttatttt gtttattata ccttttttgt gtgaaaaaat aaaggagtat ttgtgcaaaa
     3901 ttacaggctt ttcttgacag tttataattt ctttctgatt ttttgaaaat acttcatggt
     3961 atgcatatac agttgtataa aatgaaaggg agcgctatta gtttgtgaaa aaagtttgac
     4021 aaaaatagtt caaaattgat tttggatact atatctttga tttaaaaaag acaggaggag
     4081 aatataagcg agaaaagaag aatgatggaa ataagagtga aattatgaag aaaaacggaa
     4141 gatatttgat agaatgagaa tattttgtat aaaatgacga aaaaatagaa aaaccaacta
     4201 gtaattgaat gtcaaaatac gttaaaatta taacgaaata aaaatggaca tataaaacaa
     4261 aaatagaatt ttaaagataa aatagagaaa aacaggaaaa atacacaagc attcctgaaa
     4321 aaaacaggag attcagaaag aaaattgaaa gaattgttaa aaaatttgca aataggcaga
     4381 atgttcattt tgtttagtgg aatgggcaaa ataaaataaa tgttaaataa ctaacagaaa
     4441 atggttgcta acatgataat aatttgttat tattacatag aatgaaagag caacaaggta
     4501 tacaatatag catttttatg gaggaaagaa tatgggtaat tcaaaaatga atgccagaga
     4561 gcgcattgat tatctgtttg atgaccacag ttttgtggaa gttggtggat atgtttccgc
     4621 tcgtaatacc gattataatc tcggtgcaaa gaaagtagaa ggtgatggtg ttatcactgg
     4681 atacggagtt attaatgacc gcatggttta tgtttacagt caggatgctt ccgcacttgg
     4741 aggttccgta ggtgagatgc atgcgaagaa aattgcttcc ctttatgatc tggcgatgaa
     4801 aacaggcgcg ccagtcattg gaatgctgga ttgtgcagga cttagattac aggaagcgac
     4861 agatgccctt cacgcatttg gaacgatttt taataagcag gcacaggcat ctggaatgat
     4921 tccacagatt tctgcagtat ttggaatgtg tggaggagga agtgccgtaa tgtccgctat
     4981 gtctgatttt acattgatgg agaaggagaa cggcagtctc tttatcaaca gtccgaatgc
     5041 attagcgggg aatgaagaat cccgtcagaa tacggcagga gcacagttcc agtctgagca
     5101 ggctggtaac gtagattttg tatgtgagac agaagaagga cttcttgacc agcttcgtat
     5161 tctcgttgat gtacttcctt ctgattatga agcagaagat gtttactttg ataacgatga
     5221 tgatttaaat agggttattc ctgagttaaa tgatgccgca tatgatgccg cttacatttt
     5281 aaaatctatt tctgacaaca atatgtacat agagacaaag gcagcctatg ccaaagagat
     5341 ggtgacggca tttatccgtc tgaatggaga gacagtaggt gcggttgcta accagccagt
     5401 agatggtgag tccgttttaa caagaaatgg tcttgaaaaa gcagaaagat ttattaagtt
     5461 ctgtgatgca ttttcgattc ctcttcttac tgtaacgaac gtgacaggac ttcgtgccaa
     5521 cgcacatgaa gagagaaagc aggcgctcgc attagcgaag tttacaacaa cacttgcaca
     5581 ggttagtgta cctcgtatta atctcattgc aggcgaggct tatggaactg cagcggaagt
     5641 aatgaattct aaagctatcg gagctgactt tgttctggca tggccacagg catctgtagg
     5701 aatgatggat gcagaacagg cagttcgtat catgtatgca gaggaaatca atacagataa
     5761 gaataagaca gcacttatcg cagagaaaac tgcggaatat gcagaaggac agagcagtgc
     5821 attagcagct gcaaagagag gatatataga tgacatcatc gagccggacg caacaagaaa
     5881 gcgtcttatc gctgcttatg aaatgcttta cggaaagaaa gtaactccag tgacaaagaa
     5941 gcatacggca gtatagtgag gtgagagtat gaacgtattt gaacaagcct tattaaatac
     6001 tttgatgggt atgggaactg tatttgcagt actgattttc atttctttac tgatttctct
     6061 ttttgtatat atcccatcta ttgaaagagc gttaaagaat cgttcttcta agaaagagaa
     6121 aaaggctgcg caggaagaaa gaccggctcc aaagcgtccg attcttgaag aagttgtgga
     6181 agaagaggaa cttgtggatg atggtgaact tgtcgcagta atcacagcgg caatcatggc
     6241 agcgaatgga ggagcagcag tcagtgcaga taagttggtc gttcgttcaa tcaagagagt
     6301 gaagagaaga tagcaaaaat gtattcttgg aatcttttta taaagttttg aaaagttagc
     6361 tatagaaaga ttttagaagt acataaaatc attcaggagg atttaaaatg aaaaattata
     6421 gaattacagt taatggtgtt gcttatgatg tagcagtaga agaattaggt gcaggagaag
     6481 caagtgcagc gccggtaagt acaccagcag ccgttccggc tccagctaag aaagctgccc
     6541 caaagacttc cggaagtgcc ggtgccgtaa agattacagc accaatgcca ggtaagatcg
     6601 tagcagtaaa ggcacaggca ggtgcttctg taaagaaggg cgacgctgtt cttgtattag
     6661 aggctatgaa gatggagaat gaaatctgtg cagcacagga tggtgttatc gcaagtgtgg
     6721 aagtagcagt tggagatatg gtagaaggcg gagatgtact ggctacaatg aactagagca
     6781 tatttgcaaa ataaaacgga ggaagattta tgagttatgt tgtagagaca atgacgaatc
     6841 tcgcagcaca gaccggattt gcattcttaa agccaggtaa cttcattatg atactggttg
     6901 ctttagtctt cttatatctg gccatcgcca aaggatatga gccacttttg ttagtgccga
     6961 tttcttttgg tatgctgctg gttaacttat atccgtccat tatggaagaa ggcggattat
     7021 tacattattt ttacttatta gatgaatgga gtattctccc ttcactgatt ttcttaggtg
     7081 ttggagcact aacagacttt ggtccgctga ttgccaatcc agcaagtttc ttgctgggag
     7141 ctgcggcgca gtttggtatt tacagcgcgt atttccttgc aatgcttatg ggatttaacg
     7201 gaaaggcagc agcagcaatt tccattattg gtggagccga tggtcctact tctattttcc
     7261 ttgccggaaa gcttgggcag acagacttaa tgggaccaat tgcggtagcg gcatattctt
     7321 atatgtcact tgtaccaatt attcagccac cgatcatgaa gctccttact acagaaaagg
     7381 aacgtcagat taagatggaa cagttaagaa ccgttacaaa aacagagaaa attatgttcc
     7441 ctatcgttgt aacagttgtt gtatgtctga ttcttcctac aacagcacca ttagttggta
     7501 tgctgatgct cggtaacctt tttagagagt ccggcgtagt aaaacagctt tcagagacag
     7561 cgtctaacgc tcttatgtac atcgtagtta ttattcttgg tacatccgtt ggtgcgacaa
     7621 caagtgcaga ggcattctta aatgtcagca caatcaagat tgtagtatta ggtcttgtag
     7681 cttttgctgt tggtactgca gcaggtgtcc tgtttggtaa acttatgtgt aaggttacag
     7741 gtggtaaggt aaatccatta attggttctg ccggggtatc tgccgttcct atggcagccc
     7801 gtgtatccca gaaagttggt gcggaagcag atccatcaaa cttcttactg atgcatgcga
     7861 tgggaccaaa tgttgccgga gttatcggta cagcggtagc cgccggaaca tttatggcaa
     7921 tttttggagt ataatttaga attatttgat tatttaaaat tcattattta tagggctaca
     7981 atctgtaaaa aatgttacag gggatattta taaatagttt acaagttgaa ttttagataa
     8041 taacagttgt aagcgcaaat agttacgtga tttgaaataa aaggagatat tatggccgaa
     8101 attaagaaaa aaccggttaa aatagtagaa accgttctcc gtgacgcaca tcagtctctg
     8161 attgctacaa gaatgacaac agaggagatg ctgccaatcg tagataagat ggatcaggtt
     8221 ggttatcatg cagtagaatg ctggggaggt gcaacatttg atgcctgcct gcgtttctta
     8281 aaggaagacc catgggagag acttcgtaaa ttaagagatg gctttaagaa tacaaagtta
     8341 cagatgttat tccgtggaca gaacatctta ggatataagc catatccaga cgatgttgta
     8401 gaatatttcg tacagaaatc tattgcaaac ggaattgatg ttattcgtat ttttgactgt
     8461 ttaaatgacc ttagaaactt acagactgct gtagatgcaa caaagaaaga gggaggacac
     8521 gcgcagatcg ccctctccta tacaatcggt gatgcatata cattagacta ctggaaaaag
     8581 actgctaaga atattgaaga gatgggagca gattctatct gtatcaaaga tatggcagga
     8641 cttttagtgc cgaatgaagc aactcgcctt gtaactgcat taaagcaggc aacagacatt
     8701 cctatcgaac ttcatacaca ctatacaagt ggtgtcggtg gaatgactta cttaaaagca
     8761 gtagaagcag gagtcgatat cattgataca gccatatctc catttgcaat gggaacaagc
     8821 cagccggcaa cagaagttat ggcggagaca ttccgtggta cagaatttga cacaggattt
     8881 gaccagaagc ttttaggtga aatcgcagat tacttccgtc cgatccgtga gaaatacatt
     8941 gcaaatggct taatgagtcc aaaggtactt ggtgtaaaca tcaagacttt aatgtatcag
     9001 gttccaggtg gaatgctttc taaccttgta tctcagttag agcagcaggg agcatccgat
     9061 aagttctatg aagtattaga ggaagttcca aaagtacgta aagacttcgg tgaaccacca
     9121 ttagttacac cgtccagtca gattgtcgga acacaggctg tattaaatgt acttaccgga
     9181 gagcgttata aaatgattac aaacgagtca aaagcccttc ttcgtggaga atacggtcaa
     9241 accgtaaaac caatgaataa agaagtacag aaaaaggcaa tcggcgatga aaagccaatt
     9301 acctgtcgtc cagccgacct tcttgaaccg gaattatcta agatcgaggc agagatgagc
     9361 cagtggaaag aacaggacga agatgtgctt acatacgcat tgttcccaca ggtagcaacc
     9421 gaattcttta aatacagagc ggcacaggag aataagattg atcctgcaaa agccgatgta
     9481 aagaatggag catatccggt gtaggattgg attgatttag attaaaaaca gacgaacccc
     9541 cgcaacttta tagatagcac atgtgaagat ttcgctgcgt agaaaatgct cagccgcatt
     9601 tttacttgct ccgacatcac atttgctatc tataaagttg tgggggttct gttttttaac
     9661 ctaaacttgc cggaaatgga taggtgaagc tatcgtatta gctgccttgt taagtatatt
     9721 ataatctatg attgcagtat taccaaatga aagaattata gccattaacg agattgaatt
     9781 ttgtttaaaa gagcatcctg caaaatctgt gaaaagttta aattcaatcg caatgcttcg
     9841 ttattcaacc attcagggat tgttaaagtt tttttgatag atttattatg atactttcga
     9901 ttatattcca aataatcata tgggatgatt acgacaactc catcatcaac atgtgtgatg
     9961 ttagacggtt ctggaagttc tttatggtcg ttaatgtcat cagttaagat aagtcctaaa
    10021 gcatctatag ccatatcata ggcttcttgc atattttctc cctgagttag acattcggga
    10081 aagtcaggaa aagtcaccca ataaccgcct tcttctgcaa tatgaaatac agcaggataa
    10141 aataattttt tcataatgga tacctccaat gtatgtgagt tattatagat gaaataaggc
    10201 tattttaagc ccgcttgttt taggatggca tcttctagtc ttttttttaa atctttgcta
    10261 tgaagaggaa ctgttgtttg tttgccagtt ttaaaattct tcattttcac atgggaacca
    10321 ttttgactta tttttatgaa accatttttt tttaataatc ttatcatttc ttttggtttc
    10381 attggcatag tatgccaccc ctttctataa ttatattata atacgtatag tatacgtgtg
    10441 tcaagaactc ggtgatacaa ctggtgagga atagtatatc atatttaaga agaaaaacaa
    10501 ctaataaatg tannnnnnnn nnaataacgc agtttcatat gctatggata gtcaggtgat
    10561 ttttcagatg tttttttcaa ttgtatgtat agcggcagct ataggtgtat ccattttggt
    10621 tcttcgcttt atgaggaagt ttatagattt tttgttacaa taggggtaat agaagtaagg
    10681 aagggatatt aggtcccttc ctttaaaaat tttaaagaaa atttttggcg atgtcatcta
    10741 aagttctgga gatttttgaa aaatcctggt ttaaatctaa tgcaagaaca gaaatctgat
    10801 ttccgctcat atggtaggtg ttattaggaa taatatcttc atcggtttgt gcataaagaa
    10861 gcattcctga tacagtatgt tcatagtttc tcaattcaaa ttccttattt tttacatagg
    10921 taaaaatctg ataaagatta ttggaatgca gagtatgtat tccgtattgt tgctgggtca
    10981 tatgcgaata atatttggca tcaataataa gtatgttatt gtttttggat aacataatat
    11041 ctgtctgcat cctaggaagc atttgatttt cagtatcatc aagctgccat gcaatttgtg
    11101 atgcattggc ggtgagttct ggatgttctt tccgataata ttcaagcaag aatttttcat
    11161 ataagcgaca cattcgttgt ccgtcaaaaa aatccatgag tttagtagtg ccgttatttt
    11221 gtgttgtcag aagaccctta tagactagat agcacatacc gattaacatt tgatagtttt
    11281 gattagagcg attatactgt tgattccaat ttacaaaatg caggtcaatt tcattaacgt
    11341 cagaaaaaaa tagcaatagt tttcttagac tcttttttcg gctgtttgta atattcgctt
    11401 taagaagcag taatacagtg gatttaataa tttggttaaa ttgtatattg gttgagaatt
    11461 catcataggt acaaatcatt tgttttttga gcattgtctg ggtcttgatg gattctgaaa
    11521 tgttaagttt tccctgtagt gtagaaagag attcggattt tgatacataa tctcttctta
    11581 aaccatgttt taattgaata ccaattcctt tatcaagaat cgctgcacat aattctgcag
    11641 tattatgaaa gttttctgtt gctaagtctt tgtaattctc tgcctgtaat gtttgaaaag
    11701 cataggacag catatagtaa atattttgaa tgggaatcat ttgacggtac tccttaatct
    11761 atcagaccag tcgatgattt tttcaggttc atcaaaccaa tattctttta aaagaggaat
    11821 caattcatat tctataatgg atgtaagtgt tcggacagta gctgttttgg cagttagtcc
    11881 acaaaaatag ctatgcccga tacaaaatcc ttctccaaga gaaatatctg ctgctatttt
    11941 tgaatttaat tgttttatgc aagaaatcaa ttttttaaaa gcatcacttt ttaaagaatc
    12001 ttggtaagtc tgaaaaccaa tagtattaaa tcccggtttc atagtaaaaa atgaaaaacg
    12061 tcgtcttaaa gcataatcta acatggcaag gctacgatca gcagtattca tcatgccgat
    12121 aatatataca ttgggtggta cagaaaaatt ctcatcagaa tataataact ggagttcaat
    12181 accacgtttg tctttttcaa tcaacataaa taattcaccg aatattttac ttaaattacc
    12241 gcgattgatt tcatcaataa taaagaaata atcattttca tcgtcatcct cagcaagttt
    12301 gcagaatttg taaaaagacc cctttttgat tgtaaaccca ttttctgtcg gacgatatcc
    12361 ctcaataaaa tcttcatagg aatagctctg gtggaactga atcatttgaa cacggtcagg
    12421 attttttgca ccaataatag aataggcaag acgtttggca gtaaatgttt ttccaacacc
    12481 aggtgcaccc tgtaaaatga tatttttctt catttttaag acatttacca atgtttcata
    12541 atcttgctca ctcatatata catcggaaag aaaatcagca gaagaatatt ctggatagtc
    12601 tacttcaggt tgtgtatcta catcatccaa ttcatcagga gtaaagatag taataagttt
    12661 ttcgatatag tcagtatatg aggtaatatc agttaagcgt ttggcaacag acttaccggg
    12721 atgttcccat tcgccaattt gaagccactt tacggtacgg atatttttat attcctggcg
    12781 agaatcatca aaaatataat ctccggtgac aataccttta ccaacaagag tattacttcg
    12841 ctttgcaaaa atgacatcat tgatcgccag cttgtttgca aaattccatg ccattaaagc
    12901 ctgattcttt cgagaggtac tgttgtcata agtgcttatc aattcctgac gaagagattc
    12961 cttagaatca tattgagaat aatctcctat gtcattcatg ccgagaacca taatttcttt
    13021 ttgctggcat tccatccaac tggtatcgtc aaagaccgtg tacatccagt agtgaagttt
    13081 atgttttttg tcggaaagcg tattagtttc agaatcatta gatttattat tgtgattagt
    13141 ggatcttccc caaatatgac caaaaacaat accaggaata ttacattttt ttgaaaacag
    13201 tgcgatttgt cccatgcgta taaaaggaat ctcgcttggt gtttgattta gaaaatgtaa
    13261 tacatccaat tgtatatctt gtccataaaa tggtgcaaac aatattggaa ataacatttg
    13321 ataatatttc attcgccata ctgtattaat tccagaaata tgttccagtt gtttgtagag
    13381 ttgttcataa tctgctgtgg aatttaaagg accaaaggaa gatattattt cagcaccttc
    13441 taccaaatca ttacgtattt cttctgcctt ttggattgcc tcattttcag taagctttac
    13501 aggttttgca ggtgagccac aagtccagct ttgtgttttt ttgtgaaaga aaagtccaaa
    13561 tttataagct gctccaccgg caatacttcc aaatatttcg cgcatatctt tgtccatttc
    13621 aagtacataa caaagattca atttatttcc tttttcattt tcgcctgttt atccgcaaaa
    13681 tccattttac ttctctctca ttttatgtta tgtttatata cgattatgcc attttttatg
    13741 gctgtttttt gagtgtagag aatggaacaa aaaaacaata agacataata tattttttga
    13801 aggagttgta aacgagaatg tcagagattt attttgacaa tggcgcgacc acaagggcgc
    13861 tgccgcaggt gcgtgagatt atggatgaag tacttgagaa ggagtacggc aatccttctt
    13921 ctatgcatat gaaaggattt gatgcagaga agtatgtcaa tcatgcgaaa gagattattg
    13981 caaagagcct gaaagttgat ccaaaggaga tttattttac ttctggcggt acagaggcga
    14041 ataatcttgc attgatcggt actgcatttg caaataaaag agagagaaag cacattatta
    14101 caagctgtat tgaacatgct tctgtatata atccattatc ttttttggaa gatgagggat
    14161 ttgaagtgac ctatcttcct gtcgatgaac atggaatcgt agatttagag gcattaaaga
    14221 aggcgcttcg taaagataca ttgatggttt ctattatgtg cgtgaataat gagattggtg
    14281 ccatcgaacc ggttgaggag attggaaaga ttgtaaagtc ttttgatcca aagattttat
    14341 ttcataccga ttgtattcag gcatatggta agttgaattg ttaccctaag aaatggaaag
    14401 cagatatgat ttctgtaagt ggacataaga ttcacggccc aaagggaatc ggatttattt
    14461 atattaagaa tggaacaaag ataaagccaa tcatctgggg tggaaatcaa cagaagggaa
    14521 tgcgttccgg aacagaaaat gtaccgggaa ttgcagggct tggtaaggcg gcagagttga
    14581 tttatgagaa tcatgaggaa aagatcgcac atatcagaga gataaaagat cattttataa
    14641 aaagagtgat ggaagagatt ccagatgtga aggataattc cggagatgct ccgcatgtgg
    14701 cgagtatcag ctttttagga gtaagaagtg aagtgcttct tcacgcatta gaagaacgtg
    14761 agatttatgt atcagcaggt tctgcctgtt catctaataa accggaagtc agcggtactt
    14821 tgaagggcat tggtcttaca aaggaatact atgaatcgac gcttcgtttt tcattctcta
    14881 tttttaatac ggtagaagaa gcagatattt gtgtggaagc gttaaaagaa ctgttgccga
    14941 tgcttcggag atttacaaga cgataaaaca attagaacat aatgtactct aggaatcttt
    15001 ttgagcttga ttttgtgaca aaaaagacta cgagagtaca tcatataaaa aaggagatat
    15061 ttatgaaatt caaagcattt ttgattaaat atgcagaaat tggaatcaag ggaaacaatc
    15121 gccgtttctt tgaagatgcc cttgtccgtc agatgaaata tgcattaaaa ccagtaggag
    15181 acttttctat cagaaaggaa tctggacgta tttttgtaca tgccaatgaa gagtatgatt
    15241 ttgatgaggc ggtggaaagc ttaagccatg tatttggtat tgcaggtatt tgtccaattg
    15301 tagtagaaga agataaagat tttgaaaaga ttgcggaagt cgtagaaaaa tatgtagatg
    15361 aagattatgc agataagcat ttttctttca aagtacatgt aagaagagca gacaagaagt
    15421 atccaattcc gtctatggaa gcagctgccc gtcttggaga gagattatta gagaagtttg
    15481 aggacttaag tgttgacgtg cataatccag atgttatgct tacagtcgag attcgtgata
    15541 aggtttacat ttattccaga gagattccgg gaccgggtgg aatgccggtt ggtacaaacg
    15601 gaagagcaat gcttcttctt tccggtggta tcgacagccc ggtagctggt tacatgattt
    15661 ccaagcgggg tgtaagtatt gacgcaactt acttccatgc acctccttat acaagtgaga
    15721 gagcgaaaca gaaagtagta gatcttgcaa aagagattgc aaaatataca ggtcctatca
    15781 cattacatgt tgtaaacttt acagatattc agatggcaat ttatgagaaa
//
