LOCUS       c00059_gnlPro..        10435 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MLCGPKPE_59.
ACCESSION   c00059_gnlPro..
VERSION     c00059_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 23:57:13
            Original ID  :: gnl|Prokka|MLCGPKPE_59
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10435
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..10435
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[542:6660]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      543..6660
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..10435
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..10435
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             complement(543..1583)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 167.1;
                     E-value: 1.2e-51)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg59_1"
                     /sec_met_domain="ETF (E-value: 1.8e-52, bitscore: 167.1,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 5.2e-39, bitscore:
                     121.6, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VNRMNLEQYKGVFIYAQQVDNKIDAIALELLGKANDLAADLGTDV
                     TAVLIGSDVKGLVDELAEYGADKVIVVDDPELKEYRTEPYTHALASVINEYKPDILLVG
                     ATAIGRDLGPRVSARVATGLTADCTVLEIGEFKARGDKEPRQGQLLMTRPAFGGNTIAT
                     IACPNHRPQMATVRPGVMQKKEPVAGAKAEVIEYNPGFTPNDKYVEILDIVKSVTETVD
                     IQAAKILVSGGRGVGSAENFALLDDLAEALGGTVSCSRAVVDNGWKPKDLQVGQTGKTV
                     RPNVYFAIGISGAIQHVAGMEESDIIIAINKDPDAPIFDVADYGIVGDALKIVPKLTEA
                     IKAQVK"
     CDS             complement(1600..2385)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 163.8;
                     E-value: 1.3e-50)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg59_2"
                     /sec_met_domain="ETF (E-value: 1.8e-51, bitscore: 163.8,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VKIVVCVKQVPDTKGGVKFKPDGTLDRAAMLTIMNPDDKAGLEAA
                     LRIKDETGAEVTVVTMGLPKAEEVLREAMAMGADKGILVTDRVLGGADTWATSTTIAGA
                     LRNIDYDLIITGRQAIDGDTAQVGPQIAEHLDLPLISYAQDIKVEGDSVIVQRQYDDRY
                     HVLKAKMPCVITALSELNEPRYMTPGGIFDAYDAEITTWGRKDLKDVDDSNLGLAGSPT
                     QIAKASDKVRKGKGVMMTAETAEEGVEYIMDKLLTKHVI"
     CDS             complement(2400..3554)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg59_3"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.1e-52, bitscore:
                     167.5, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 4.3e-31, bitscore:
                     96.4, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.1e-41, bitscore:
                     131.3, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFTLSKKHEMARQLFKEFAENEVKPLAQEVDETEHFPEETVAKM
                     QKLGFMGIPVPKEYGGQGCDPLTYIMCVEELSKVCGTTGVIVSAHTSLCADPILTYGTE
                     EQKQKYLVPLAKGEKLGAFGLTEPGAGTDAQGVQTKAVLDGDEWVLNGSKCFITNGSYA
                     DYYIIIAITSVDTDARGRKKKKFSAFIVEKGTPGFTFGTKEKKMGIRGSATYELIFQDC
                     RIPKENLLGPMGKGFAIAMHTLDGGRIGIAAQALGIAEGALDATIAYVKERKQFGRAIA
                     AQQNTQFQLANMATQVEAAKLLVYKAAMAKATQRVYSVEAAKAKLFAAETAMDVTTKCV
                     QLLGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGNLLK"
     CDS             complement(3655..4494)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg59_4"
                     /sec_met_domain="HCDH (E-value: 5.7e-39, bitscore: 122.1,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 4.9e-62, bitscore: 198.3,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVGVIGAGTMGSGIAQAFAQTEGYEVYLCDINDEFAANGKKKIA
                     KGFEKRVARGKMDQAKADAILAKITTGTKDICTDADLVVEAALEVMEVKQQTFKELQDI
                     VPATCMFATNTSSLSITQIGAGLDRPVIGMHFFNPAPVMKLIEVIAGLNTPDEMVEKIK
                     AISVEIGKTPVQVEEAAGFVVNRILIPMINEAVGIYADGVASVEGIDTAMKLGANHPMG
                     PLALGDLVGLDICLAIMEVLYNETGDPKYRPHPLLRKMVRGGKLGQKTGIGFYDYSK"
     CDS             complement(4563..5348)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: EC1_2-faecali"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg59_5"
                     /sec_met_domain="EC1_2-faecali (E-value: 3.9e-92, bitscore:
                     297.3, seeds: 67, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGFIDYEVDGQVGIITINRPKALNALNSEVLKDLDATIDAVDLDA
                     IRCLIITGAGEKSFVAGADIGEMSTLTKAEGEAFGKAGNDVFRKIETLPIPVIAAVNGF
                     ALGGGCEISMSCDIRLCSENAVFGQPEVGLGITPGFGGTQRLARIVGPGKAKQLIYTAR
                     NIKAAEAYRIGLVNEVYPLEELMPQAKKMAKGIAKNAPIAVRACKKAINEGLEVGMDEA
                     IVIEEKLFGSCFETEDQKYGMAFFLDKNKEKVKEPFKNC"
     CDS             complement(5479..6660)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg59_6"
                     /sec_met_domain="Thiolase_N (E-value: 2.8e-104, bitscore:
                     337.3, seeds: 30, tool: rule-based-clusters)"
                     /sec_met_domain="Thiolase_C (E-value: 5.6e-51, bitscore:
                     160.7, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKKVVLAGACRTAIGTMGGGLSTVPAADLGSIVIKEALKRTNVP
                     ADQVDEVLMGCVIQAGLGQNVARQASINAGLPIEVPAVTINVVCGSGLNCVNLAATKIL
                     AGEADIVIAGGMENMSLAPFCLDKARFGYRMNNGVLKDCMVNDALTDAFNQYHMGITAE
                     NVAEQWHLTREELDEFSANSQQKATAAIEAGKFKDEIVPVEVKLKKKTVVVDTDEGPRP
                     GTTAESISKLRPAFKKDGIVTAANSSGINDGAACVVVMSEEKAKELGVTPMATWIAGAL
                     GGVDPSIMGVGPVASTKKVLAKTGMSIDDFDLIEANEAFAAQSLAVGRELGINPEKLNV
                     NGGAIALGHPVGASGCRILVTLLHEMQKRDAKKGLATLCIGGGMGCSTIVERD"
     CDS             complement(7181..7393)
                     /locus_tag="ctg59_7"
                     /transl_table=11
                     /translation="MLVSNTCSEATDRLIASFTIAGFEGNKVKILRCATIRCWGECVNL
                     KYRFFGIDFVNIITMIKTNCNTKYT"
     CDS             complement(7498..9195)
                     /locus_tag="ctg59_8"
                     /transl_table=11
                     /translation="MNTLVIVLIAAVLLTAAYAFYGRWLSKKWGIDPKAETPAHKFEDG
                     QDYVPTDGWTVFSHQFSSIAGAGPVTGAIQAAVFGWLPVLLWVLIGGIFFGAVTDFGAL
                     YASVKNDGKSMGLLIEKYIGKLGRKLFLIFCWLFTLIVIAAFADMVAGTFNAYTVDANG
                     VIALSDAAKTNGAAGTISLLFIAFAMIFGLLHKHLHLTGWKETVVGLICTVAALAIGMT
                     MPITLGKDGWTYITFVYIFFAAVLPMWLLKQPRDAMTTYMFIGMIAGAVVGLLVAHPAM
                     NLPVFTGFHNDQLGDLFPILFVTVACGAVSGFHSLVSSGTSSKTVSNEKDMLKVGYGAM
                     VLESLLAVIALCVAGAAASANGTPADGTPFQVFSSGVAGFFEMFGIPVYVAQCFMTMCV
                     SALALTSLDAVARIGRMSFQELFSVDDMEHAEGWRKVLCNTYFSTIITLAAAFVLTRIG
                     YANIWPLFGSANQLLSALVLVTLCVFLKVTGRSNKMLFPPLIIMLCVTGTALVERTVSL
                     VQAFSAGSATFLVEGLQLIIAILLMILGVIIVVNSLRAYFASKKNSEI"
     CDS             complement(9541..10134)
                     /locus_tag="ctg59_9"
                     /transl_table=11
                     /translation="MDKIGHLVMTKSLSGNIHRAHKAALLFGCILPDLLVYTYLEGHTW
                     EATFEQITKQMKSLEAKGRGGSFSYLKLGWILHYVEDYFTYPHNTIFEGTIPEHYAYEK
                     KMTKWMREGALEQMSLPSCKKLDSAAEVEERLQELHDRYLSQKMCYENDMAYMRQMVSE
                     ILNCYAEIFVRKSEFAHFMEWVRKKVGIMTGFVS"
ORIGIN
        1 gtcaactgaa tgaaggcaaa atatagcgca aattggggcg tgcagattgt aggaatgatt
       61 tttcaaacac gctctagtat agcatctatc taatttctaa aatattattg gatatagttt
      121 ttctaattta cttcgtgcat cttttattgt gaactatcaa ataattattc ataaacactg
      181 aatggatact atactagagc gtgtttgaaa aatgcttttg caagatgtgc gttacaattt
      241 gtggggaatt tttcctgaat gaagacggcg tagcgggcta tgccaactga atgaaagtaa
      301 aaaagaccac agttataaac tgtagtccta ctatctcact ttaacaggga atcttcaaaa
      361 agattccctg cataaataca ttttatagag ttcagcacaa atctcctata tgcttctttt
      421 aataatgaat cgtcaataca caaaacatct gagaatgcat atactagact tccacaaaaa
      481 atctaagttt caatcctcaa tcagtaactc acataatcta atgagtctga tatgatttga
      541 aattatttaa cctgagcttt gattgcctct gttaacttag gaacaatctt taatgcatcg
      601 ccaacaatac cgtaatcagc tacatcaaag ataggtgcat ctgggtcttt attgatagca
      661 atgatgatat cagattcttc cataccagct acgtgctgga tagctccgga aataccgatt
      721 gcaaaataaa cattaggacg aactgtctta ccagtctggc caacctgtaa atcttttggt
      781 ttccaaccat tatcaacaac tgcacgtgag caggaaactg ttccgccaag tgcctctgct
      841 aaatcgtcta ataatgcaaa gttctcagca gagccaactc cacgtccgcc agatacaaga
      901 atcttagcag cctggatatc aactgtttct gtaacagatt ttacaatgtc aagaatctca
      961 acatatttgt catttggagt aaatcctgga ttgtactcaa taacttctgc ctttgcacct
     1021 gcaacaggct ctttcttctg cataacacca ggacgaactg tagccatctg tggacgatgg
     1081 ttaggacaag cgattgtagc gattgtgtta ccaccgaatg caggacgagt cattaataac
     1141 tgaccctgac gaggttcttt atctccacga gctttgaatt caccaatttc aagaacagta
     1201 cagtctgctg taagacctgt tgcaactctt gcagaaacac gaggtcctaa gtcacgaccg
     1261 attgctgttg ctccaaccag aagaatgtcc ggtttgtatt cgttgataac agatgccagt
     1321 gcatgtgtat atggctctgt tctgtattct tttaattcag ggtcatcaac tacgataact
     1381 ttatctgcgc cgtattcagc aagttcatca actaagcctt ttacgtcaga accgattaaa
     1441 actgctgtta cgtctgttcc taaatctgca gcaaggtcat ttgcttttcc aagtaattct
     1501 aaagcaatgg catcaatctt attgtcaacc tgctgagcat agataaatac acctttatat
     1561 tgttctaaat tcattctatt caccacattt cctttacaat tagataacat gctttgttaa
     1621 cagtttgtcc atgatgtact caacgccttc ttctgctgtt tcagctgtca tcatgacacc
     1681 tttaccttta cgtaccttat cagaagcttt tgcgatctga gttggagaac ctgcaagacc
     1741 taagttgctg tcatcaacat ctttaaggtc ttttcttccc catgtagtaa tttctgcatc
     1801 atatgcatca aagattccgc ctggagtcat ataacgtggc tcatttaatt cggaaagtgc
     1861 tgtgataaca catggcattt tagcttttaa aacatgatat ctgtcgtcat actgtctctg
     1921 aacgattaca ctgtcacctt caactttgat atcctgagca taggaaatta aaggaagatc
     1981 taagtgttca gcaatctgag gtcctacctg agcagtatca ccatcgatag cctgacgacc
     2041 tgtgatgatt aaatcataat caatatttct aagtgctcca gcgattgttg tagatgttgc
     2101 ccatgtatca gctccaccta aaacacggtc tgttacaagg atacccttgt cagcacccat
     2161 tgccatagct tcacgcagaa cttcttctgc ttttggaaga cccatagtaa ctactgttac
     2221 ttctgcgcct gtttcatctt ttattctaag ggcagcttca agacctgcct tatcatctgg
     2281 attcatgata gtaagcatag ctgctctatc aagagttccg tctggtttaa acttaactcc
     2341 accttttgta tcaggtacct gtttaacaca tactacgatt ttcacagtat tttccctcct
     2401 tatttcaata agttaccaga aataaccata cgctgaactt ctgatgttcc ttcgtagatt
     2461 tctgtgatct tagcgtcacg catcatacgt tctacgtcgt actctctgat gtaaccataa
     2521 ccaccaagta actgaacaca cttagttgtt acatccatag ctgtttctgc tgcaaataat
     2581 ttagcttttg ctgcttctac ggagtatact ctctgtgttg cttttgccat tgctgcttta
     2641 taaacaagta acttagcagc ttctacctgt gtagccatat tagcaagctg gaactgtgta
     2701 ttctgctgtg cagcaattgc tctgccaaac tgttttcttt ctttaacata agcaattgta
     2761 gcatctaaag caccttctgc gataccaaga gcctgagctg caataccgat acgtccacca
     2821 tctaatgtgt gcatagcgat tgcgaaacct ttgcccatag gtccaagaag attttcttta
     2881 ggaatacgac aatcctggaa aatcaattca tatgtagctg aaccacggat acccattttc
     2941 ttttcttttg taccaaatgt aaatcctgga gttccttttt ctacgataaa tgcggagaat
     3001 ttctttttct ttcttcctct tgcatctgta tcaacacttg tgatagcgat gataatatag
     3061 taatcagcat aagaaccgtt agtgatgaaa catttagatc cgtttaatac ccattcgtca
     3121 ccatctaaaa cagcctttgt ctgaacaccc tgagcgtcag taccagcacc tggctctgta
     3181 agaccgaaag cacctaactt ttcacccttt gcaagtggaa caagatattt ctgtttctgt
     3241 tcttcagtac cgtaagtaag aattggatct gcacacaatg atgtatgtgc ggatacaata
     3301 acacctgttg taccacaaac tttagataac tcttcaacac acataatgta tgttaaagga
     3361 tcacaaccct gtccgccata ttcttttggc acaggaattc ccatgaaacc taatttttgc
     3421 atttttgcaa cggtttcttc tggaaaatgt tctgtctcgt caacctcctg agcaagaggt
     3481 ttaacttcgt tttcagcgaa ctctttaaag agctgtctag ccatttcatg ctttttgctt
     3541 aatgtgaaat ccattttcat tcctccataa taaattggtt attggggtgg agaaccacct
     3601 taaaagccgt ggagacgcag agcatagtcc ccacggccgt attcactttt ttaattattt
     3661 gctgtaatcg tagaatccaa taccagtctt ctgacctaat tttccaccac gaaccatctt
     3721 tcttaataat ggatgtggac gatacttagg atctcctgtt tcattatata atacttccat
     3781 gattgcaagg cagatatcaa gaccaactaa atctcctaaa gcgagaggtc ccattggatg
     3841 gttagcacct aacttcatag ctgtgtcgat accttcaaca gaagcaacac catcagcata
     3901 aatgccaact gcttcgttaa tcattgggat taaaattctg ttaactacga atcctgcagc
     3961 ttcttctacc tgtacaggtg ttttgccgat ttctacggag atagctttaa tcttttcaac
     4021 catttcatct ggtgtattaa gaccagcgat aacttcaatt aatttcataa ctggagctgg
     4081 gttgaagaaa tgcattccga taactggtct gtcaagtcct gcaccaatct gtgtaataga
     4141 taaagaagat gtatttgttg caaacataca tgttgcagga acgatatcct gtaattcttt
     4201 aaatgtctgc tgtttaactt ccataacttc aagagctgct tctactacta aatcagcatc
     4261 tgtacagata tcttttgtac cagtagtaat cttagcaagg attgcatcag ctttagcctg
     4321 atccattttt cctcttgcta ctctcttttc aaatccttta gcgattttct ttttaccatt
     4381 tgcagcaaat tcatcgttga tatcacataa atatacttcg tatccttctg tctgtgcgaa
     4441 tgcctgagca atacctgatc ccattgtacc tgcgccaata actccaactt tcattgtttg
     4501 aaacctccta taaaatgtaa tgtattccga taattaataa tacaatatgt ataattgatt
     4561 aattagcagt ttttaaatgg ctcttttact ttttctttgt ttttgtcaag gaagaatgcc
     4621 attccgtatt tctggtcttc tgtctcgaag cagctgccaa ataacttctc ttcgattacg
     4681 atagcttcat ccattcctac ttctaaacct tcgttgatag ctttcttaca tgcacgaact
     4741 gcgataggtg cgttctttgc aatacccttt gccatcttct tagcctgagg cattaactct
     4801 tcgagtggat aaacttcgtt aaccagaccg attctgtaag cttctgctgc tttaatattg
     4861 cgagcagtat agattaactg ttttgctttt ccaggtccta cgatacgagc aagtctctgt
     4921 gttccaccaa aaccaggtgt gattccaaga ccaacttctg gctgtccaaa cactgcattc
     4981 tcagagcaaa gtctgatgtc acagctcata gaaatctcac agccaccgcc taaagcgaat
     5041 ccgttgactg ctgcgataac agggattgga agtgtttcaa tctttctgaa tacatcatta
     5101 ccagctttac cgaaagcttc gccttctgct tttgtaagtg tactcatctc tccgatatca
     5161 gcacctgcta cgaaagattt ctctccagca ccagtaatga taagacatct gattgcgtct
     5221 aaatctactg catcaatagt tgcatctaaa tcttttaata cttcactgtt taaagcattt
     5281 aaagcctttg gacgattgat ggtgataata ccaacctggc catctacttc ataatcgata
     5341 aatcccattg tatgtatctc ctttcagatt acattagcta actattcaat ctagataatt
     5401 aaatagttac aaactatgta attgatataa ccggattcga aaaccgaacc cggatatatg
     5461 taatcattct atacgtgatt agtctctttc aacgattgtt gagcagccca taccaccacc
     5521 gatacacagt gtagcaagac ctttcttagc atcacgtttc tgcatctcat gtaataatgt
     5581 aacgaggata cggcatcctg atgctcctac tggatgtcca agagcaatag ctccaccatt
     5641 aacatttaac ttctctgggt tgattcctaa ttctcttcct actgctaatg actgagctgc
     5701 gaaagcttcg ttagcttcga ttaagtcaaa gtcatcaatg ctcatgcctg tttttgcaag
     5761 aactttctta gtagaagcaa ctggtccaac acccatgata gaaggatcaa ctccgcctaa
     5821 tgctcctgct atccatgtag ccattggagt tacacctaat tctttagctt tttcttcgct
     5881 cataacaaca acacaagcag caccatcgtt aatacctgaa gagttggcag ctgttacgat
     5941 accatctttc ttgaaagcag gacgtaattt agaaatactt tctgctgttg ttccaggacg
     6001 tggtccctca tctgtatcta caacaactgt tttcttttta agtttaactt ctacaggaac
     6061 aatctcatct ttgaacttac cagcttcgat agctgctgta gctttctgct gtgaattagc
     6121 ggagaactca tctaactctt ctcttgttaa atgccactgt tctgcaacgt tttcagctgt
     6181 aatacccata tggtactgat tgaaagcatc tgttaatgca tcgttaacca tacagtcttt
     6241 taatacgcca ttgttcattc tgtatccaaa acgagctttg tctaagcaga atggagctaa
     6301 tgacatgttt tccataccac cagcgataac gatatcagcc tcacctgcta aaatctttgt
     6361 agcagcaagg ttaacacagt taagaccgga accacaaact acgttaattg ttacagcagg
     6421 tacttcgatt ggtaagcctg cattaataga agcctgacga gcaacgttct gtccaagacc
     6481 agcctggata acacatccca ttaatacttc gtctacctga tcagcaggta cgttagttct
     6541 ctttaacgct tctttaatta cgatgcttcc taaatctgca gctggtactg tgcttaatcc
     6601 accacccatt gttccgattg cggtacgaca agcacctgct aatacaactt tctttgccat
     6661 agtttcattc ctccatttaa gttatcaata ttatttgata gttgctttgc tagagcattt
     6721 ctagtacgat atgtgttaat cttatcacaa tcttctttgc tttgcaacaa gattataaaa
     6781 aaatttttaa ttaatatttt catttatgaa agattcacag tttcgtcact tttgaataca
     6841 aaatccatcg ttgttttaat taattttacc tttatttctt cttatatcta tggacattct
     6901 ctattgatta attttctgat tttgtgaaaa aaggaaacaa aatacaaaaa actgcctaaa
     6961 tatgtgaaga aatagacatt ctgagttggc taatagacga aaaaccagag atactttacc
     7021 tgccagatgt ggctttggag tgagttttat ggcattttgc acagtgaaat aaagcatatg
     7081 gtacataaag tatctctggc ttttctatct gttggaagga tttagtttag aatgtctata
     7141 tggagggatt tgaaaattgc ccggatagct gtagatgaaa ttatgtatat tttgtattgc
     7201 aatttgtttt tatcattgta attatattaa caaaatcaat accaaaaaat ctatatttta
     7261 aattcacaca ctctccccaa catcgaatcg ttgcacatct aagaattttt actttgtttc
     7321 cttcaaagcc agcaatagta aagctcgcta taagtctatc tgtggcctcg gagcaagtgt
     7381 tggatacgag catataagcg agtattcaac acgttgcgac taaagtcaca tatatggatt
     7441 attttgatta tatagttcgt ctgttcgtct gtttctatac tttgcctgtc tggcgaatta
     7501 aatttccgaa ttcttctttg aggcgaagta agcccggagt gaatttacta cgatgatcac
     7561 tccaagtatc atcagtaaaa tagcgataat cagctgtaag ccttctacta aaaatgttgc
     7621 ggaaccagca gaaaatgcct gtacgagaga aacggttctc tctactaatg cggttcctgt
     7681 tacacaaagc atgataatca gtggtgggaa aagcattttg ttgcttcgtc cagttacttt
     7741 taagaatacg caaagtgtta ctaatactaa agcagataag agctggtttg cggaaccaaa
     7801 taatggccag atatttgcat aaccaattct tgttaataca aaggctgctg ctaacgtgat
     7861 gattgttgag aagtatgtat tacataaaac ttttctccat ccttctgcgt gttccatatc
     7921 atcaacactg aacaattcct gaaagctcat tcggccaatt cgcgctacgg catccaaact
     7981 tgtaagtgca agcgcggata cgcacattgt catgaagcac tgtgctacat atactggaat
     8041 tccaaacatt tcaaagaatc cggctacacc gcttgagaat acctggaatg gtgttccatc
     8101 tgctggtgtt ccgttagcgg acgctgccgc tcctgctaca cataaagcga taactgcaag
     8161 taaactttct aataccattg cgccgtaacc aacttttaac atatcttttt cattggatac
     8221 cgtctttgaa gatgttccgg aagatactaa actgtggaaa ccagaaactg ctccacaagc
     8281 aacggttacg aataaaatag ggaataaatc accaagctga tcattatgga agcctgtaaa
     8341 tacaggaaga ttcattgccg gatgcgctac taataatcca acaacagctc ctgcgatcat
     8401 tccgataaac atataggttg tcatcgcatc acgaggctgt tttaataacc acattgggag
     8461 tactgccgca aagaaaatat atacaaatgt aatatatgtc cagccgtcct ttcctaatgt
     8521 aattggcatt gtcataccaa tagcaagtgc tgctacagta cagattaatc caacaaccgt
     8581 ttccttccat cctgtcagat gcagatgttt atgaagtaaa ccaaaaatca ttgcaaatgc
     8641 gataaataaa agggaaattg ttcctgctgc tccatttgtt tttgctgcat cggaaagtgc
     8701 aatcacacca ttggcatcta ccgtataagc attaaatgta cctgctacca tatcagcgaa
     8761 agctgcgata acaattaaag taaacagcca gcagaaaata agaaataact ttcttccaag
     8821 tttaccgatg tatttttcga tcagaagtcc catggactta ccatcatttt ttacactggc
     8881 atataatgct ccaaaatcgg taactgctcc aaagaaaata cctccaatta atacccacaa
     8941 aagtactgga agccatccaa atactgccgc ctgaattgct cctgttacag ggcctgctcc
     9001 agcaatagat gaaaactgat ggctaaatac tgtccaaccg tcagttggaa cataatcctg
     9061 tccatcttca aacttatgag ctggtgtctc tgcttttggg tcaattcccc attttttaga
     9121 aagccaccgt ccataaaatg catacgcagc tgtcaataaa acagcagcaa ttaaaacaat
     9181 gactaatgta ttcatttttg tcctctcctt tcatgtatac aagccatttc ttgtatacat
     9241 gaagttttat aatccttttc cttcattttg tcaatgtttt ttgcgttttt tcttgcattt
     9301 ttataggaac aaataaattt taaagtcaat tcacttcatt tctagatttt tatgcaagaa
     9361 gttctgaact tttttaagca aaagatacta gagcgtgttt gaaaaaaaca tttttcaaac
     9421 acgctctatg gcttctttta ggaagtcagg caggaatcct ctgtaattca atcgccgatt
     9481 atttcacaaa ataagtttcc catatttagc tgaactatat aaaaattttt caaacacact
     9541 ctaagaaaca aatcccgtca ttattccaac tttcttccgc acccattcca taaaatgtgc
     9601 gaactcgctc tttctcacaa aaatctcggc ataacaattc aatatttccg aaaccatctg
     9661 ccgcatatat gccatatcat tctcataaca cattttctgt gaaaggtatc tgtcatgtaa
     9721 ttcctgaagt ctctcctcca cttccgcagc agaatcaagc ttcttacagc tcggcaggga
     9781 catctgctct aaagctccct cccgcatcca ctttgtcatt tttttctcgt aagcataatg
     9841 ttctggaatt gttccctcaa aaatcgtatt atgcggatag gtaaaataat cttccacata
     9901 atgaagaatc catccaagtt ttaaataaga aaagcttccg cctcttcctt ttgcctctaa
     9961 cgatttcatc tgttttgtaa tctgttcgaa cgttgcttcc catgtatgac cctccagata
    10021 agtatacacc agcagatctg gcagaataca tccaaacaat aaggctgctt tatgtgctct
    10081 gtgtatattt cccgataatg actttgtcat aactaaatgt ccaattttat ccattcattt
    10141 ctcctcattc actttcaacc tggtatcatt tctacattta aaattaaatt ccattcacta
    10201 cctctcaaaa cacagtatac cataattata aaaaatggct acaaccaaaa tatgttattt
    10261 tactatttaa ttcattaagc aaactacaga cgaagccatt gcccccaata aggcatattt
    10321 gtgattttag tcgcggcgtg ttgaatgttc gctctattcg ctcgcatcca acacttgctc
    10381 cgagaccaca aatatgcctt attgggggca atggcttctg tgctgtggct ggaga
//
