LOCUS       c00219_gnlPro..         3339 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MLCGPKPE_219.
ACCESSION   c00219_gnlPro..
VERSION     c00219_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 23:57:13
            Original ID  :: gnl|Prokka|MLCGPKPE_219
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3339
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3339
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[96:3337]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      97..3337
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..3339
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3339
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(97..1623)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg219_1"
                     /sec_met_domain="HGD-D (E-value: 5.9e-64, bitscore: 203.9,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYHSRLYAAANYAKKNKQLEVIQLNSFGCGLDAVTTDQVNDIMTN
                     SGRIYTVLKIDEVNNLGAARIRIRSLIAAVKIRNRKHIEPHPVPANIEKVEFTKEMKKD
                     YTILIPQMSPIHFDLLTPAFRHCGYNVEILPEMGKQAVDTGLKYVNNDACYPSLIVVGQ
                     IMTALTSGKYDLNKTAVIISQTGGGCRATNYIGFIRRALAKNGYGQIPVISLSVQGIEK
                     NEGFKFTPALLVRALQGVIYGDLFMRVLYRVRPYEKVKGSANHLHEVWKKRCIKSLEKA
                     RYGEFKKNIRGIIRDFDRLPITDEKKPRVGIVGEILVKFLPDANNHLIELLEEEGAEAV
                     MPDLLDFMFYSFYNSNFKAEYLGKSTKAAKLCNMAISALEAYRGFLRKELERSDRFTAP
                     PHIEELAEYAKPIVSLGNQTGEGWFLTGEMVELIKQGAPNIVCTQPFACLPNHVVGKGV
                     IKELRRKYPESNIVAIDYDPGASEVNQVNRIKLMLATAEKKLKSDLPQAR"
     CDS             complement(1682..3337)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg219_2"
                     /sec_met_domain="BcrAD_BadFG (E-value: 5.2e-33, bitscore:
                     101.7, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SLLYRFYDNNNGSPLNTTIRAMKELKELLPPAAKISGSCSTGYGE
                     ALIKAALQLDHGEVETMAHYYAAAFFEPDVDCILDIGGQDMKCIKIRNHAVDNVLLNEA
                     CSSGCGSFIETFAKSLNYSVADFAKVALFAPSPIDLGSRCTVFMNSKVKQAQKEGASVG
                     DISAGLAYSVIRNALLKVIKLTDPKQLGKKIVVQGGTFYNDAVLRSFERISGCHAVRPD
                     IAGIMGAFGAALIAREREEETGDTQMLSIDEIINLEYSTSMSRCQGCNNHCILTINKFN
                     NDRQFISGNRCERGLGLEKNHEHVPNLYEYKYKRIFGYKALKPDEAKRGVIGIPRVLNM
                     YENYPFWYTFFTNLSFRVLLSPKSSRKIYELGIESIPSESECYPAKIAHGHVMWLLKKG
                     INTIFYPCVPYEEDQMEGANNHYNCPIVTSYAENIKNNMEELRDPNVLFMNPFLALDNA
                     DALKVRLYDELSKHFDVTKEEIDHAVDEAYKEAAQVRKDVQAKGEETLAYLAETGKMGI
                     VLCGRPYHIDPEINHGIPELINSFGIAVXXXXIK"
ORIGIN
        1 atatacaccg ggcaacacaa aaattttata tttttttaat tgtctacttg acgggatgca
       61 caccactttc attctcagag ggcgtttgtt tggcgtctat cttgcctgcg gcaaatcaga
      121 tttcaacttc ttctccgctg ttgccagcat caatttaatt cggtttacct ggtttacttc
      181 gcttgctccc ggatcgtagt caattgcgac gatattagat tccggatact tacggcgcag
      241 ttccttgata acacccttac ctacaacgtg gtttggcagg caggcaaatg gctgtgtaca
      301 tacaatgttt ggggcaccct gtttgataag ttcaaccatt tcaccagtta agaaccatcc
      361 ctcaccggtc tggttaccga gggatacgat tggttttgcg tactcggcaa gttcctcaat
      421 gtgaggtggt gcagtgaaac gatcgcttct ttctaattct tttcgaagga agcctcggta
      481 agcttcaagt gcggagattg ccatattaca taacttagca gcttttgtag attttccgag
      541 atattctgct ttaaagttac tgttgtagaa ggagtagaac ataaagtcta ataaatctgg
      601 cattactgcc tctgccccct cttcctctaa aagttctatc agatggttgt ttgcgtctgg
      661 aaggaatttt acgaggattt ctccaacgat accaacacga ggtttcttct catctgtgat
      721 tggtaatctg tcaaagtcgc ggataatgcc gcggatattt ttcttaaatt ctccatatcg
      781 tgctttctca agagatttaa tacaacgctt tttccatact tcatgtaaat gatttgcaga
      841 accttttact ttttcgtatg ggcggacgcg gtagagaaca cgcataaata aatctccgta
      901 aataactccc tgaagagcac gaactaaaag tgccggagtg aatttaaaac cttcattctt
      961 ctcaattccc tgtacgctta aactgataac tggaatctga ccgtaaccgt tcttagctaa
     1021 tgcacggcgg atgaatccga tatagtttgt cgcacgacag ccaccaccag tctgggagat
     1081 gataacggct gttttgttca gatcgtattt gccggaagtt aaggcagtca taatctgtcc
     1141 aacaacgata agagatggat agcaggcatc gttatttaca tattttaatc cagtatctac
     1201 tgcctgtttc cccatttccg gcaaaatctc tacgttatag ccacagtgac ggaaggcagg
     1261 ggtgagcagg tcgaaatgaa ttggggacat ctgaggaatc agaatggtgt agtccttttt
     1321 catttctttt gtaaattcta ctttttcgat atttgctgga accggatgtg gctcaatgtg
     1381 cttacggttt ctgatcttta cggcagcaat cagagaacgg atacggattc tggcagcacc
     1441 taagttattt acctcatcaa tcttaaggac ggtataaata cgtccggaat tcgtcataat
     1501 atcatttacc tggtcagtag ttaccgcatc aagaccacag ccaaaggagt taagctgaat
     1561 gacttctaac tgcttgttct tctttgcgta gtttgcggca gcgtacagac gggaatgata
     1621 catccactgg tcagaaacga ttaatggacg ttcaacatga cctaagtgac agatggaatc
     1681 ttcacttaat nnnnnnnnnn aatacggcga tgccgaaaga gttaatcagt tcaggaatac
     1741 cgtggttaat ctctgggtcg atatggtaag ggcgaccaca aagaacaata cccatctttc
     1801 cagtttctgc gagataagca agagtttctt cgccctttgc ctgtacatct tttcttacct
     1861 gtgcagcttc tttataagct tcatctacgg catggtcaat ctcttctttt gttacatcga
     1921 aatgtttaga taactcgtca taaagacgta ccttcaaggc atccgcatta tcgagtgcta
     1981 agaatgggtt cataaaaagg acattcggat cacggagctc ttccatgttg tttttaatat
     2041 tttctgcata agatgttacg atcgggcagt tgtaatggtt gttggcacct tccatctgat
     2101 cttcctcata aggaacacaa ggatagaaaa tggtgttgat acccttcttt aaaagccaca
     2161 ttacatgtcc gtgtgcaatc tttgcaggat agcattcgga ttcacttgga atggactcaa
     2221 taccaagctc gtaaatcttt cgggaagact ttggagaaag caatacccgg aaagaaaggt
     2281 ttgtaaagaa tgtataccag aatggatagt tctcatacat attcagtact cgtggaatac
     2341 cgataacacc acgttttgcc tcgtctggtt ttaatgcttt atatccaaaa attcgtttat
     2401 atttatattc gtaaaggttt ggtacatgct catgattctt ttcaaggcca agaccacgtt
     2461 cacaacggtt accagaaata aactgacggt cgttgttaaa cttattaatg gtaaggatac
     2521 agtggttgtt acatccctga cagcggctca tggaagtgct gtattccaag ttgataatct
     2581 cgtcgatgga gagcatctgt gtatctccag tttcttcctc acgctctctt gcaattaagg
     2641 ctgcaccgaa agcacccatg ataccggcaa tatctggacg aacggcgtga cagccggaaa
     2701 tacgttcaaa gcttcgaagt accgcatcat tatagaatgt accaccctga acaacgattt
     2761 ttttaccaag ctgtttgggg tcggttaact taataacttt taataaggca ttacggataa
     2821 cggaataagc aaggccagca gaaatatcac caacgcttgc gccttctttt tgtgcctgtt
     2881 ttactttgga gttcataaat acagtgcagc gggaacctaa gtcgattgga gatggggcaa
     2941 acagcgcaac ctttgcaaaa tcagctacag aatagttcag agattttgcg aaagtttcga
     3001 tgaaagaacc acatccagaa gaacaggcct catttaaaag tacgttatct acagcgtggt
     3061 tacgaatctt gatacatttc atatcttgtc cgccgatatc taaaatacag tctacatctg
     3121 gctcgaagaa agcggcagca tagtaatgtg ccattgtctc tacttcgccg tggtcaagct
     3181 gcagtgcagc cttaattaat gcttcaccgt aaccagtaga acaggaaccg gaaatcttcg
     3241 cagcaggagg aagtaactcc tttaactctt tcatagcacg aattgttgta tttaacggac
     3301 ttccgttatt attatcgtag aaacggtata agagggaac
//
