LOCUS       c00335_gnlPro..         3098 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CAACBNMM_335.
ACCESSION   c00335_gnlPro..
VERSION     c00335_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 03:01:44
            Original ID  :: gnl|Prokka|CAACBNMM_335
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3098
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1653
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg335_1"
                     /sec_met_domain="HGD-D (E-value: 2.6e-18, bitscore: 53.9,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 1.8e-66, bitscore:
                     211.4, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GFGKTLLEGVMEGKIKELVLVSCCDTIRSVYDILLESGKLDFLYI
                     LDVLHCDSACSRERMAVQLKGLAKAYAQYKGTEFDAGKFRAAFHAQEKITKSHIAVLGA
                     RMGQELFEMTSKAMPLPVENDTCVHNRSVGNILPPEGASFDELMDWYAGEILGQIPCMR
                     MMDPTGRKKLYNDPSVAGIIYHTVKFCDFYSFEYAEIKNHTDVPLLKIESDYTIQSSGQ
                     LLTRLEAFAESIQPETLEQTIDGDTKGERKMGKGYFAGIDSGSTSTDVVILNKDHEIVT
                     SIILPTGAGAAIGADRALAEALKEAGLQREDIDALVTTGYGRTAIKNGDKSITEITCHA
                     RGAHFLNPEVRTVIDIGGQDSKVIRLDENGAVANFVMNDKCAAGTGRFLEMMARTMEMN
                     LDQMSECGLEFKEDITISSMCTVFAESEVVSLIAQNKATDDIVHGLNKAVAVKTAALTR
                     RVGGEEKYMMTGGVSKNKGLVKTLEEKLGTKLVISDKAQLCGALGAALFAXXXXXXXXX
                     XXXXXXXXXXXXXXXXXXXXXXXXNFYTFFIKV"
     protocluster    1..3098
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:1653](+)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..1653
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..3098
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3098
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             2082..3098
                     /locus_tag="ctg335_2"
                     /transl_table=11
                     /translation="MWEAINSFLTTVDNLVWGVPLMVLIMAGGILLTARLGLLQMRRLP
                     LALKWMFKNEEEGDGEISSFAALCTALSATIGTGNIVGVATAVCAGGPGALFWMILAAF
                     FGMATKFSEGLLAVKYRVVAEDGHSLGGPFYYIEKGMGTKWKWLAKIFAFFGVCVGLFG
                     IGTFSQVNGISSAVQNFFDPNKSNCVNIPFLGEYSWAVVISSLILAACVAAILIGGLKR
                     IATVSQIIVPFMAVIYLIFSVALILLNITEIPAAIVTVVKGAFNPSAVTGGVVGTMIVS
                     MQKGVARGIFSNEAGLGSAPIAAAAAQTKEPVRQGLVSMTGTFIDTIVICTMTGLSIV"
ORIGIN
        1 ggttttggaa aaacacttct ggaaggagtc atggagggga aaatcaagga acttgtcctg
       61 gtgagctgct gtgatactat ccggagtgtc tatgacattc tgcttgaaag cggcaagctg
      121 gattttctct atattttgga tgtgcttcac tgtgacagtg cctgtagcag agagcgtatg
      181 gctgtacagt taaaagggct ggcgaaggct tatgcacagt acaaaggcac agagtttgat
      241 gccggaaaat tccgggctgc tttccatgca caggagaaaa tcacaaagtc ccatattgca
      301 gtgcttggcg ccagaatggg gcaggaatta tttgaaatga ccagcaaggc tatgcctctt
      361 cctgtagaga atgatacctg cgtgcataac cgttctgtgg gaaatatact tccaccggag
      421 ggtgcttctt ttgatgagct gatggactgg tatgcaggtg agattctggg gcagattcca
      481 tgtatgcgta tgatggaccc gacaggtcgt aagaagcttt ataatgatcc gtctgttgcg
      541 gggattattt atcatacagt gaagttttgt gatttttaca gcttcgaata tgctgagatc
      601 aagaaccata cagatgtacc tcttttaaaa attgaatcag attatactat tcagagcagc
      661 ggtcagcttc ttaccagact ggaagctttt gcagagagca ttcagcctga aacactggag
      721 cagaccatag atggagacac aaaaggagag agaaaaatgg gaaaaggtta tttcgcaggg
      781 attgacagtg gttccaccag tacagatgtg gtgattttaa ataaagatca tgagattgtg
      841 accagtatta tcctgcctac aggagcagga gctgctatcg gagcagaccg tgcccttgca
      901 gaagcattga aggaggccgg attgcagaga gaagatattg atgcgctggt tactaccgga
      961 tacggacgta cagctattaa aaatggtgat aagagtatca cagagatcac ctgtcatgca
     1021 aggggtgcgc atttcctgaa tccggaagtc cgtactgtta ttgatattgg cggacaggac
     1081 agcaaggtga tccgactgga tgagaacggt gcagttgcaa actttgttat gaatgacaaa
     1141 tgtgcggcag gaaccggacg atttctggaa atgatggcac gtaccatgga aatgaatctg
     1201 gatcagatga gcgaatgtgg gctggaattc aaagaagata ttacaatttc cagtatgtgt
     1261 acagtatttg cagaatcaga agttgtatcc ctgatcgccc agaataaggc aacagatgat
     1321 attgttcatg gtctgaataa ggcggttgcc gtaaaaaccg cagctcttac cagacgtgtg
     1381 ggcggtgagg agaaatatat gatgaccggt ggtgtttcta agaataaggg cctggtaaag
     1441 acactggaag agaagcttgg aacaaagctg gtgatcagtg acaaggctca gctttgcggt
     1501 gcacttggcg cagcactgtt tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnaattttt atactttttt tataaaagtt taattttcta ttgacgagtt caaatttaag
     1681 agctatagta taccacagta acagcaaatt aacgaggtga agtttgcagg aaagaggaag
     1741 ccacacaaaa ggctataccc gccgaaggag taaaactctc aggttccctg gatgcaggga
     1801 agaactgcaa atggatcgtt tctctggaga cacccgttag atcgggggcc gaaggtgcaa
     1861 ataacacgga atgctgataa cttcccacaa ttggttatct gacccgtgtc ttgaatctct
     1921 caggcaaaag gacagaggat gctttatcat ccccgctgat tcatatgaac tatatgaaca
     1981 gtattttctg aacttctgat ttttgaaacg gtctgttatt actttgtttc ccagctgtac
     2041 aatatattac actttttaaa agaaaaagga gatttattat tatgtgggaa gcgatcaatt
     2101 cttttttgac aacagtagac aatctggtct ggggcgtgcc ccttatggtc ctgatcatgg
     2161 caggtggcat cctgttaact gccagacttg gacttcttca gatgcgaaga ctccctcttg
     2221 cattgaaatg gatgtttaag aacgaagaag aaggcgatgg tgagatttcc agctttgcag
     2281 ctttatgtac agcactgagc gccacaatcg gaaccggtaa tatcgtcggt gttgcaactg
     2341 ctgtctgtgc cggaggtccc ggagctttat tctggatgat tcttgcagca ttcttcggaa
     2401 tggcaacaaa attttccgaa ggtcttcttg cagtgaaata ccgtgtggta gcagaagacg
     2461 gtcacagcct tggcggtcct ttctattata ttgagaaagg tatgggaaca aaatggaaat
     2521 ggcttgcaaa gatctttgca ttctttggtg tatgtgtagg tcttttcgga atcggtacat
     2581 tcagccaggt aaatggtatt tccagtgcag ttcagaactt ctttgatccg aacaaatcaa
     2641 actgcgtaaa tattccgttc cttggagagt attcctgggc agttgtaatt tcttccctga
     2701 ttcttgcagc ctgtgttgca gcaatcctca tcggtggtct gaaacgaatt gcaacagtca
     2761 gccagattat tgtaccgttt atggcggtga tctatctgat cttcagtgta gcgctgattc
     2821 ttttaaacat tacagagatc ccggcagcta tcgtaacagt tgtgaaggga gcctttaatc
     2881 cgtcagcagt gacaggcggt gtggtaggaa caatgatcgt atccatgcag aaaggtgtgg
     2941 ctcgtggtat cttttcaaat gaagcaggtc ttggtagcgc acctatcgca gcggcagcag
     3001 ctcagacaaa agagcctgta cgtcagggtc ttgtaagcat gactggtaca tttattgata
     3061 cgatcgtgat ctgtacaatg acaggtcttt ccatcgta
//
