GCA_934850335.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaGMEFPFMP_4 (original name was: gnl|Prokka|GMEFPFMP_4)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	NRPS	21,376	70,360	dipeptide aldehydes	NRP	47%

gnlProkkaGMEFPFMP_8 (original name was: gnl|Prokka|GMEFPFMP_8)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	lanthipeptide-class-ii	66,706	87,915

gnlProkkaGMEFPFMP_16 (original name was: gnl|Prokka|GMEFPFMP_16)

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	ranthipeptide	38,873	60,461

gnlProkkaGMEFPFMP_38 (original name was: gnl|Prokka|GMEFPFMP_38)

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	cyclic-lactone-autoinducer	9,295	24,799

Compact view

gnlProkkaGMEFPFMP_4 - Region 1 - NRPS

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Location: 21,376 - 70,360 nt. (total: 48,985 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 21375 - 70360:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg4_44

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaGMEFPFMP_8 - Region 1 - lanthipeptide-class-ii

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Location: 66,706 - 87,915 nt. (total: 21,210 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg8_81

allorf_75552_75623

Leader:

MNICNYRIKRILMGMSVFA

Core:

CISD

Core with dehydrations:

CysIDhaD

allorf_87694_87879

Leader:

MSLNFSTYAYITKSPVRYSDAAVFSA

Core:

AAPDYHHPELFPVLLLFHTRELYPHMTEVTCFQTL

Core with dehydrations:

AAPDYHHPELFPVLLLFHDhbRELYPHMDhbEVDhbCysFQDhbL

antiSMASH-DB 4.0 matches for allorf_87694_87879

14.3% NZ_CP058552.1: Lanthipeptides: allorf_1481579_1481740_lanthipeptide

23

YPHMTEVTCFQTL

35

Y H+ + C QT+

6

YKHVQKTNCVQTI

18

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

allorf_75552_75623 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 418.2 Da

Molecular weight: 418.5 Da

Number of bridges: 1

allorf_87694_87879 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 4093.1 Da

Molecular weight: 4095.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4113.7 Da N = 1; 4131.7 Da N = 2; 4149.7 Da N = 3

gnlProkkaGMEFPFMP_16 - Region 1 - ranthipeptide

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Location: 38,873 - 60,461 nt. (total: 21,589 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaGMEFPFMP_38 - Region 1 - cyclic-lactone-autoinducer

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Location: 9,295 - 24,799 nt. (total: 15,505 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.