LOCUS       c00104_gnlPro..         3161 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IFFOJIKN_104.
ACCESSION   c00104_gnlPro..
VERSION     c00104_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 23:15:47
            Original ID  :: gnl|Prokka|IFFOJIKN_104
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3161
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..681)
                     /locus_tag="ctg104_1"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRMEDDLKEDPADFAVWKAAKPGEPAWESPYGMGRPGWHIECSAMSRTHLGK
                     TIDLH"
     protocluster    1..3161
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1100:3159]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1101..3159
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3161
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3161
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(1101..1853)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.3;
                     E-value: 1.9e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg104_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.2e-30, bitscore:
                     94.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKPLGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQLDFLTEFFTLARAKGVHTA
                     LDTAGQPFRPDDPAYLAAFDRLMANTSLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWIRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEELLEVSRYPG"
     CDS             complement(1840..3159)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg104_3"
                     /sec_met_domain="Gly_radical (E-value: 6.2e-43, bitscore:
                     133.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.6e-136, bitscore:
                     442.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FARIPSYNQLFSGDPVWATLEVGGIGMDGRSMVTKNCYRFLHTLE
                     NMGPAPEPNLTVLYSSALPEAFKKYAAKVSVNTSSVQYENDDVMKPVWGDDYSICCCVS
                     ATQTGKEMQFFGARANLAKCLLYAINGGVDEKSHEQCGPNYAPITSEYLTYDEVLPKYV
                     QMLDWLAGLYVNVLNLIQYMHDKYYYEEAEMALIDTDVRRTFATGIAGFSHVIDSLSAI
                     KYAKVKVVRDETGLATGYEVEGDFPKYGNDDDRADEIGVWLLRTFLEMIKKRHTYRNSE
                     ATTSILTITSNVVYGKYTGALPDGRAAFTPFAPGANPSYGAEQNGLLASLNSVAKLPYH
                     WALDGISNTQTINPEALGHSEDERVENLVQVLDGYFDQGAHHLNVNVFGKEKLLDAMEH
                     PEKEEYANFTIRVSGYAVKFIDLTREQQLDVLARTCHGVL"
ORIGIN
        1 gtgcaggtcg atggtcttgc ccagatgggt gcggctcatg gcgctgcact cgatgtgcca
       61 gcccgggcga cccatgccgt aggggctttc ccatgcgggc tcaccgggct ttgcagcctt
      121 ccagacggca aagtcggcgg ggtcttcctt cagatcgtcc tccatgcggc tgcgtagctc
      181 gcggttgccg ctttccagat cgtccagatt caggtggctc agcttgccgt agccggggtc
      241 gctcttcacc cggaagtaca catcgccgtt tttggcaacg taggcgtggc cggagtcgat
      301 cagatccttg acgatatcca aaatctgggg gatatgctcg gtagcgcggg gctgcacggt
      361 gggcttttcg cagttcagac cggcggcatc cttcaggtac tcggcctcaa agcgctgcgc
      421 cacctccttc atggaggtgc cctcttcctg tcccttcttg atgagcttat cgtcgatatc
      481 ggtgatgttg gaaacgtaga tcaccttgtt gccccggtat tccagatagc ggcgcagggt
      541 gtcgaacacg atcagcgggc gtgcgttgcc gatgtggata tagttgtaca cggtagggcc
      601 gcagacgtag atgcggtact cgccgggcac ctgcggcaca aattcttcct tctgtcgggt
      661 cagggtgttg aagatctgca tggtcaattc tcctttttgt tccaaacaga agccgcaaaa
      721 caaaacgccc ccggagcggt gcacaaggca cagctccgaa ggcggtttta aaatcaaaat
      781 cgcggttcca cttctgtttg tcgctcaaag ccggtaacgg cggcgcaccg cacggcgata
      841 cttccccacg gggttcccgc cgcgtgctgt ccgggcgcac tttgcgcggc ggtctgcaaa
      901 caggcttcca gccagtggcc tgttctctct gcgcagacgg tgtgcggcgc tactctgccc
      961 ggataaacgc atttatcctt ttaccccttt attatagcgg tgcggtgcag ggaagtcaag
     1021 gcagaacaaa cctgtgaaaa tgaaattcag ccaagcctac cggcttggct gaatttcaaa
     1081 tcaaaaagat gctttcctct ttacccggga taccggctca cttccagcag ttcttctgcc
     1141 cgctttacct gctctgccgt gggcggtaca gcgtccggca ggggataggg gatacccagc
     1201 ttctgccact tgaacagccc caaggtgtgg tagggcagca cctccacccg ctgcaccgtt
     1261 ttgagcgagc ggataaagtc agccgttttg cgcagcccct cctcgtcatc ggtcagcccg
     1321 ggcacaagga cgtgccggat ccacagcggg atgccgaggt cggagatgtg ccgcgccatg
     1381 gcaaggatgt tggcgttgtc tttgccggtg agctgccggt ggcgctcggg gtcgatctcc
     1441 ttgaggtcaa ggatcaccag actggtgtta gccatcagac ggtcaaatgc cgccagatag
     1501 gccgggtcgt ccgggcggaa gggctgcccg gcggtgtcca gcgccgtgtg cacgcccttg
     1561 gcacgcgcca gcgtgaagaa ctcagtcagg aaatccagct gccgcagcgg ctcgccgccg
     1621 ctgaccgtga tgccgccctt tttgccccag tagttgcggt agcggtatac ccgctggaac
     1681 agcgcttctg ccgtccacgc ctcgccgccc tctgcccatg tttcggggtt gtggcagtac
     1741 ttgcaccgca gggcacaccc ctgcaaaaac accacaaagc gcacgccggg gccgtccacc
     1801 gagccgaagg attccagtga gtggacgtac ccaaggggct tacaggacgc catggcaggt
     1861 gcgtgccagc acgtccagct gctgctcgcg ggtcaggtcg atgaacttga cagcgtagcc
     1921 ggaaacgcgg atggtgaagt tggcgtactc ttccttctca gggtgctcca tggcgtccag
     1981 cagcttctcc ttgccaaaga cattcacgtt caggtggtgt gcgccctgat cgaagtagcc
     2041 gtccagcacc tgcaccagat tctccacgcg ctcgtcctcg ctgtgaccca gtgcctcggg
     2101 gttgatggtc tgggtgttgg agatgccgtc cagtgcccag tggtagggca gctttgccac
     2161 cgagttcagg gaagccagca ggccgttctg ctctgcgccg tagctggggt ttgcaccggg
     2221 tgcaaagggg gtaaaggcgg cacggccatc gggcagagca ccggtgtact tgccgtacac
     2281 cacgttggag gtgatggtca ggatggaggt ggtagcctcg gagttgcggt aggtgtggcg
     2341 cttcttgatc atctccagga aggtgcgcag cagccaaacg ccgatctcat cggcacggtc
     2401 gtcatcgttg ccgtatttgg ggaagtcgcc ctctacctca tagccggtgg ccaggccggt
     2461 ctcgtcgcgc acgaccttga ccttggcgta cttgatggcg ctcagggagt cgatgacgtg
     2521 ggagaagcct gcaatgccgg tggcaaaggt gcggcgcaca tcggtgtcga tgagtgccat
     2581 ctcggcttcc tcgtagtagt acttgtcgtg catatactgg atgaggttca gcacgttgac
     2641 gtacagcccg gccagccagt ccagcatctg cacatacttg ggcagcacct cgtcataggt
     2701 caggtactcg ctggtgatgg gggcatagtt ggggccgcac tgctcgtggc tcttctcgtc
     2761 cacaccgccg ttgatggcgt acagcaggca cttggccagg ttggcgcggg caccgaagaa
     2821 ctgcatctcc ttgccggtct gggtggcaga cacgcagcag cagatggagt aatcgtcgcc
     2881 ccagacaggc ttcatcacgt catcgttctc gtactggacg ctgctggtgt tgacagacac
     2941 cttggcggcg tacttcttaa aggcctcggg cagggcagag gagtacagca cggtcaggtt
     3001 gggctccggt gcggggccca tgttttccag tgtgtgcagg aaacggtagc agttcttggt
     3061 gaccatgctg cggccgtcca tgccgatgcc gcccacctcc agcgttgccc acacggggtc
     3121 gccggagaac agctggttgt agctggggat gcgggcaaac t
//
