LOCUS       c00253_gnlPro..         3145 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FBGOKMGM_253.
ACCESSION   c00253_gnlPro..
VERSION     c00253_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 00:27:01
            Original ID  :: gnl|Prokka|FBGOKMGM_253
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3145
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3145
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1343:3144]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1344..3144
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3145
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3145
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..883
                     /locus_tag="ctg253_1"
                     /transl_table=11
                     /translation="DNKEWICSICGRQNKEEFKYCPECGEKKPDHEETQTEITIKKLKR
                     KGFENCEQLFRLLIHVEDEEGNLLGSYNMTLHGYATKQELLNLLEDYLGCKENHRAPKK
                     YHLEIQDWPYHSLDSAYETKEDFHVCELYSAEWNAVRKEYGEKRTDHEETWEEIRIKQK
                     DYANCEQKYKVLVHLEDEEGNPLGNYNVMLCEYDTEKELIDLLEDYWGYKKNIYKEYYL
                     KVIDWPHNPGPSEWWPGSSLHVCEMEEREFRVIRKKYKEDDGPMPCLYGCPNSRDIEEK
                     KEVETKVEIVYF"
     CDS             complement(1344..2096)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.4;
                     E-value: 3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-32, bitscore:
                     101.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTECNKNAMALVHSTESFGSVDGPGVRFIVFLQGCPLRCQFCHNP
                     DTWKMAKENGAVWRSADELLNQALRYRTYWKNSGGITVSGGEPLLQIDFMLEFFKKAKE
                     KGIHTVIDTAGGPFTREEPFFSKFQELMKYTDLLLVDIKHIDTECHKTLTGRSNENILD
                     MIRYLSDIKKPIWIRHVLVPERSDKDEYLTRLADFIHSLDNVEKVEILPYHTMGIYKWK
                     ELGLEYPLEGIEPPTKERVENAKKILAI"
     CDS             complement(2083..3144)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_3"
                     /sec_met_domain="Gly_radical (E-value: 1e-43, bitscore:
                     136.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.1e-93, bitscore:
                     300.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EVMKPEWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAING
                     GVDEKTGQQVGPEYKPITSEYLDYDEVMHKYDIMMDWLAGLYVNTLNLIQYMHDKYYYE
                     YALMALIDTNVRRTFATGIAGFSHVVDSLSAIKYAKVKTVRNEEGLVVDYETTGDFPKY
                     GNDDDRADDIAVWLLQTFMKKLEKFHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKA
                     GEPLSPGANPSYGAEQNGLLASLNSVAKLPYEWALDGISNTQTINPDALGHSEEERINN
                     LVNVMDGYFAQGAHHLNVNVFGVEKLIDAMEHPEKEEYANFTIRVSGYAVKFISLTKEQ
                     QLDVIARTCHDRM"
ORIGIN
        1 ggataataag gaatggatat gtagtatttg tggaagacaa aacaaagaag agtttaaata
       61 ttgtcctgaa tgcggggaga aaaaaccaga tcacgaagaa acacaaacag agataactat
      121 aaagaaatta aaacgaaaag gctttgaaaa ctgtgaacaa ttgtttagac ttcttattca
      181 tgtcgaagat gaggagggta atctattagg aagttataac atgacgctgc acggatatgc
      241 cacaaaacag gaattgctaa atttgttaga ggattatctg ggatgtaaag aaaatcatcg
      301 tgcccctaaa aaatatcatt tagagataca agattggcca tatcattctt tagatagtgc
      361 atatgagaca aaagaagatt tccatgtatg tgagctctac agtgctgagt ggaatgctgt
      421 cagaaaagaa tatggtgaga aaagaacaga ccatgaagaa acatgggaag aaataagaat
      481 aaaacaaaaa gactatgcaa actgcgaaca aaaatataaa gtgcttgttc atttagaaga
      541 tgaagaaggc aatccactgg gaaactataa tgtaatgctg tgtgaatatg atacggaaaa
      601 agagttaata gatttattag aagattattg gggatataaa aagaatatat ataaagagta
      661 ttatctaaaa gttatagatt ggccacataa tcctggtcca agtgagtggt ggccgggtag
      721 tagtttacat gtatgtgaaa tggaagagag agaatttcgt gttatcagga aaaaatataa
      781 agaagacgat ggtccaatgc cttgccttta tggttgtccg aattcaagag atatagaaga
      841 aaagaaagaa gtggaaacaa aagtagagat tgtttatttc tgatttgtag tgctaaacag
      901 acgccgttgt ttagggcaat gtagccggct ttttagaagt gtttagatag gaaacataaa
      961 acaatagcct attttgttcc gttgtgctaa atgctccatt ttgcccagta aagcaggaac
     1021 ttggcctcaa aacctccact ggagcttttt tcgatctgct aaggcaggag aagggagtcc
     1081 caagttccta tggtcaaaat tccgcagcac aaagtcgcaa aataggctat gttttatgtt
     1141 tctttttcct atattctttg taaaatccgg aaaaaaattt ttgtaacgtc tgtttctcta
     1201 aatcttgcaa aaaatcctgc aaattaatgg aacaaaatac aaccttagaa gaatccctct
     1261 gttagaagtc atcgatgcaa tccgaccata aatatctcca cagctgcgtt tgtcatcaac
     1321 tttacaaatc agaaattgat agatcagata gcaagaatct tcttagcgtt ctccactcgt
     1381 tcttttgttg gtggctcaat tccctccagt ggatactcta atccaagttc tttccattta
     1441 taaattccca tcgtatgata aggcaggatt tccacctttt ccacattatc aagagagtga
     1501 atgaaatcag ctaatctagt cagatattca tctttatcgc ttcgttccgg aacaaggaca
     1561 tgacgaatcc aaattggttt tttgatatca gaaagatagc gaatcatgtc gagaatattt
     1621 tcgttgcttc gaccagttaa agtcttgtga cattcagtat caatatgttt gatatccact
     1681 aataacaggt ctgtatattt catcaattcc tgaaatttag aaaagaaagg ttcctctctt
     1741 gtaaaaggtc ctccagcagt atcaataaca gtgtggattc ctttttcttt tgccttttta
     1801 aaaaactcta gcataaaatc aatctgtaaa agaggctcac caccacttac ggtaataccg
     1861 ccgctatttt tccagtaagt acgataacgg agtgcctgat ttaacagctc atccgcactt
     1921 ctccacaccg caccattttc ctttgccatc ttccatgtat ctggattgtg acagaactga
     1981 catcgcaaag gacaaccctg taaaaaaaca ataaaacgaa ctcccggccc atccaccgaa
     2041 ccaaaactct ccgtagaatg aacaagagcc atcgcatttt tattacattc tgtcatggca
     2101 tgttctggca attacatcta actgctgttc ttttgtcaga gagatgaatt taactgcata
     2161 acctgaaaca cggatcgtaa agtttgcgta ttcttctttt tccggatgtt ccatagcatc
     2221 aatcagtttc tcaacaccaa atacatttac gtttaggtgg tgagcaccct gtgcgaagta
     2281 gccgtccata acatttacta agttattgat acgttcttct tcggaatgac cgagtgcatc
     2341 agggttgatt gtctgtgtat tggaaatacc atctaatgcc cattcgtaag gaagttttgc
     2401 tacagagttt aaggaagcta aaagtccgtt ctgctcagca ccataacttg ggtttgctcc
     2461 tggggaaaga ggttctccgg cctttcttcc gtctggaagg gaaccagttg ccttaccata
     2521 tactacgttt gatgtgatag taaggatgga agttgtaggt tcagaatcac ggtaagtatg
     2581 gaatttttca agcttcttca taaatgtctg aagtaaccat acagcgatat catcagcgcg
     2641 gtcatcatca tttccgtatt tagggaagtc gccagttgtc tcataatcaa caacaagtcc
     2701 ttcttcattg cggactgttt ttactttggc atatttaatc gcacttaagg aatctactac
     2761 atgtgagaat cctgcgatac ctgtagcgaa tgtacgacgt acatttgtat cgattaaggc
     2821 catcaaagca tattcatagt agtatttgtc atgcatgtac tggatgagat ttaatgtatt
     2881 tacatataaa cctgctaacc agtccatcat gatatcatat ttgtgcatta cttcatcata
     2941 gtcaagatat tcggaagtaa ttggtttgta ttccggacca acttgctgac cagtcttttc
     3001 atctacacca ccattgattg cgtagaggag acattttgca aggttagcgc gagcaccgaa
     3061 gaactgcatt tccttaccag tctgtgtagc agaaacacag cagcagatgg agtaatcatc
     3121 gccccactcc ggtttcataa cttca
//
