LOCUS       gnlProkkaAGOJHKEG_77   15060 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AGOJHKEG_77.
ACCESSION   gnlProkkaAGOJHKEG_77
VERSION     gnlProkkaAGOJHKEG_77
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 22:04:09
            Original ID  :: gnl|Prokka|AGOJHKEG_77
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15060
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15060
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[4606:7495]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4607..7495
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..15060
                     /SMILES="NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..15060
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             2..373
                     /gene_functions="other (smcogs) SMCOG1026:transposase
                     (Score: 116.8; E-value: 1.7e-35)"
                     /gene_kind="other"
                     /locus_tag="ctg77_1"
                     /transl_table=11
                     /translation="MDTINKAKARRNIDQPLIIHSDRGSQYVAKEYKKATENMQRSYSK
                     KAFPWDNACIESFHSVIKREWLNRFKIRDYKQAYQLIFEYLEAFYNTKRIHSHCNYMSP
                     NEFERVYERTHTEAELLAG"
     CDS             519..926
                     /locus_tag="ctg77_2"
                     /transl_table=11
                     /translation="MTNEQLANSFRVLARKEQVDYSKKEYAQSNIAVIRKLWEWQKKVY
                     QNLELHLDAAIDKVDNLAKDVELQRMEKKWDELYEQTNQEGKNTVKQQTVALVSENQYE
                     YGAEAFEAYMEKQGDTVNIETTLIDKKNNVR"
     CDS             1239..1436
                     /locus_tag="ctg77_3"
                     /transl_table=11
                     /translation="MIVRLQYYNNIAVFLRSKGGTNTMVEIKTGRELLLENVLSFRGIV
                     NQKEIQQIGMDCRQLQLDIA"
     CDS             complement(1780..2031)
                     /locus_tag="ctg77_4"
                     /transl_table=11
                     /translation="MILVTAFVCETILNENVELLISNKIQPTALPNGFVSVDIDVTPMD
                     NSNSNKEGVSRTYKGFDGYAPMMAYIGTEGYAINFELR"
     CDS             2414..3199
                     /NRPS_PKS="Domain: ACPS (156-214). E-value: 4.2e-10. Score:
                     31.4. Matches aSDomain: nrpspksdomains_ctg77_5_ACPS.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012:4'-phosphopantetheinyl transferase (Score: 133.1;
                     E-value: 1.4e-40)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg77_5"
                     /transl_table=11
                     /translation="MKFIHTIGESGRTCKNTLNLLKYKFLMGWLALNFTDLGNVYWDNK
                     ETLEDKLIVNYLNVSNINADDMYILYDAASDERKQKSKIYKRKIDADICICSESLLKYT
                     IKEITGKSLIKKDISIAYEYNEFGKPYLKSNNLFFNISHSSEWIAVVCDLAEVGIDIER
                     IRPAEPRMICRLFSDEEIGYINSGLCSERHRRFTKLWTIKESYVKYIGTGLLTSLKSFR
                     VDVSNKRIIDLCDTDNYDVIKYSKETVPNYYMSVCGKNE"
     aSDomain        2882..3055
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg77_5_ACPS.1"
                     /evalue="4.20E-10"
                     /label="ctg77_5_ACPS.1"
                     /locus_tag="ctg77_5"
                     /protein_end="214"
                     /protein_start="156"
                     /score="31.4"
                     /tool="antismash"
                     /translation="VGIDIERIRPAEPRMICRLFSDEEIGYINSGLCSERHRRFTKLWT
                     IKESYVKYIGTGL"
     CDS             3275..4225
                     /locus_tag="ctg77_6"
                     /transl_table=11
                     /translation="MTEKKIVGKLKSFNEVMYKDCFYHALIPTFRHFTGFIEPLLLNDI
                     CLEYSIENQDKVPTLIMKNASSQSYSDIFEKFGISVKKSRISSKKMIDHTIEVLNNDGI
                     AIIRIDCFYEPLRIDSYQKYHAGHVIPIYGYDNTRNMFDILEGDFVDSVVYDAKKLEYE
                     HVRNAYKGYIEEFKTHNHTYYEFKKVDNSVDYKKEYEVRKNNFTEYFQSNSDVFLESLK
                     EINNYKQKLSNYFANGINDNISTIVTSFNRMLDEVVVQKYLFSKLFELPSEYSVVIDET
                     IRLWKAIRAIVTKSMSSMRLYLISANCRCNWNSNY"
     CDS             4607..7495
                     /NRPS_PKS="Domain: AMP-binding (1-358). E-value: 1.6e-73.
                     Score: 239.4. Matches aSDomain:
                     nrpspksdomains_ctg77_7_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (460-526). E-value: 1.2e-11. Score:
                     36.5. Matches aSDomain: nrpspksdomains_ctg77_7_PCP.1"
                     /NRPS_PKS="Domain: PP-binding (882-949). E-value: 5.4e-13.
                     Score: 41.2. Matches aSDomain:
                     nrpspksdomains_ctg77_7_PP-binding.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     198.8; E-value: 2e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg77_7"
                     /sec_met_domain="AMP-binding (E-value: 6.5e-80, bitscore:
                     258.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-13, bitscore:
                     41.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEIISMIVGIVKSGAAFMVIDKEFPLKRIEYMLNNSECNTVIYN
                     DLGNDEKLEKLKQNNDITFVDYKNCLKGKLQNSKEVCRKCTDIQYIVYTSGTTGNPKGV
                     MVTDENLANYIQWFISEFALTNDESSIVVSSLAFDLGYSNVFPVLAVGGTVNLLKKTQY
                     ISPVKAINYIKDNAVTFMKCTPSYFNLMMNYNDTGILDFGEKFRFLVLGGENLKIKDVS
                     KFIKDNPNIRVINHYGPTETTIGIAFSEISDLSLKKYKEKPFVGYPIINTEVFIKDDYG
                     VLHDYEDCKKNHIKGELCIQSKSTSAGYINNKELTNEKFIVNGGHKGKIYCTGDIVHIE
                     NDGLINIMGREDSQVKINGYRVEVNEIKNIMLEFASIDFAEIDVEDQDIIAYIKESKNI
                     EYDELYDFLGERLSTYMLPKRIYVVKKIALTLNGKIDFKIMKKSGKLLQSNNPITKPET
                     DMEKALYKLWKQLLNCEEEFSIEDNFFSVGGTSILGIELLSYLNLDNFEYQDLLENSSI
                     KKLACVIEMKKGSIKEIDELKMPDCLTDYVDKKEVRYMVKPFNKVYYEDCIYNAIFSAF
                     DYFNIELRYFLSHELPTLDESNFIFKRDNNMVKIDCHINKVYGEDFIKGDFVGNSNVID
                     KIRTALLNKNIPIVRIDCYYEKYRKEFYNKSHFMHALCITGFNDFEKKLYALEHSNVNS
                     LDYKEIEISYQEVINANFAYEYFTGYTRESYYEVSKIKDSSYLQKDPVRDFVQYICKHK
                     SVLKKNISKTNVVMVEGKFALDNTELFSDRLIQMRIFLKMEIFKAELLVDDEQVKNPII
                     ESNKIFEKMFQIVLVKKMRKQSLDLTETDKSNMKYALTLLREYFSKLLKWADNKVEEMS
                     TNNKEIKILENRIKELFYQVTNIDQDIDVNRKLTDYGINSITIIEFLVKIEDEFDIEFD
                     IENMTVEKFESLHDLCDYVQSNSQNLDNCKTY"
     aSDomain        4610..5680
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg77_7_AMP-binding.1"
                     /evalue="1.60E-73"
                     /label="ctg77_7_AMP-binding.1"
                     /locus_tag="ctg77_7"
                     /protein_end="358"
                     /protein_start="1"
                     /score="239.4"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="PEIISMIVGIVKSGAAFMVIDKEFPLKRIEYMLNNSECNTVIYND
                     LGNDEKLEKLKQNNDITFVDYKNCLKGKLQNSKEVCRKCTDIQYIVYTSGTTGNPKGVM
                     VTDENLANYIQWFISEFALTNDESSIVVSSLAFDLGYSNVFPVLAVGGTVNLLKKTQYI
                     SPVKAINYIKDNAVTFMKCTPSYFNLMMNYNDTGILDFGEKFRFLVLGGENLKIKDVSK
                     FIKDNPNIRVINHYGPTETTIGIAFSEISDLSLKKYKEKPFVGYPIINTEVFIKDDYGV
                     LHDYEDCKKNHIKGELCIQSKSTSAGYINNKELTNEKFIVNGGHKGKIYCTGDIVHIEN
                     DGLINIMGREDSQVKIN"
     aSModule        4610..6184
                     /complete
                     /domains="nrpspksdomains_ctg77_7_AMP-binding.1"
                     /domains="nrpspksdomains_ctg77_7_PCP.1"
                     /locus_tags="ctg77_7"
                     /monomer_pairings="Asp -> Asp"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       4874..4927
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg77_7_0001"
                     /evalue="1.80E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg77_7"
                     /protein_end="107"
                     /protein_start="89"
                     /score="26.5"
                     /tool="antismash"
                     /translation="YIVYTSGTTGNPKGVMVT"
     CDS_motif       5492..5566
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg77_7_0002"
                     /evalue="5.40E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg77_7"
                     /protein_end="320"
                     /protein_start="295"
                     /score="18.8"
                     /tool="antismash"
                     /translation="GELCIQSKSTSAGYINNKELTNEKF"
     CDS_motif       5648..5707
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg77_7_0003"
                     /evalue="3.30E-08"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg77_7"
                     /protein_end="367"
                     /protein_start="347"
                     /score="25.8"
                     /tool="antismash"
                     /translation="MGREDSQVKINGYRVEVNEI"
     aSDomain        5987..6184
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg77_7_PCP.1"
                     /evalue="1.20E-11"
                     /label="ctg77_7_PCP.1"
                     /locus_tag="ctg77_7"
                     /protein_end="526"
                     /protein_start="460"
                     /score="36.5"
                     /tool="antismash"
                     /translation="EKALYKLWKQLLNCEEEFSIEDNFFSVGGTSILGIELLSYLNLDN
                     FEYQDLLENSSIKKLACVIEM"
     aSDomain        7253..7453
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg77_7_PP-binding.1"
                     /evalue="5.40E-13"
                     /label="ctg77_7_PP-binding.1"
                     /locus_tag="ctg77_7"
                     /protein_end="949"
                     /protein_start="882"
                     /score="41.2"
                     /tool="antismash"
                     /translation="RIKELFYQVTNIDQDIDVNRKLTDYGINSITIIEFLVKIEDEFDI
                     EFDIENMTVEKFESLHDLCDYV"
     CDS             7795..8865
                     /locus_tag="ctg77_8"
                     /transl_table=11
                     /translation="MSNKTTVATIFALNYYSKGITMLESVNKFNPDIELNILISDKVEN
                     KEKFLNDALIKKLNVKVTFLEDLELRDFENLAFKYDMVEFCTCVKPKYLLMLADEYDKI
                     IYLDPDIMVYAVLEEIQNLLDVHNIVLTPHISGTIEEGISPATDFLRNGIYNLGFIAVK
                     NADVVKIFLKWWHKKLMNYCFCDSRKSLAWDQKWVDFVPALFDGVYVLRSPNYNVAFWN
                     IQGRYISEKNSTYYVNNDYPLVFVHYSHFNINSPNYLFYINDGLNLLIEDRRDLSKITS
                     EYVSALRSNTNESYAKKKYRYNYYDNGEIVKEAHRKLYNHLDTETEEYNEPFNTKKTNS
                     FYKLAKMFEDIDVSAL"
     CDS             8883..10658
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 170.7; E-value:
                     6.7e-52)"
                     /gene_kind="transport"
                     /locus_tag="ctg77_9"
                     /transl_table=11
                     /translation="MKFLEKVKYIKEFNKKRFIYEGIYCFIEYLEYILVGCLYLKWIVT
                     DVQSGKELSYILKKILGFTVILFLIFIIRSYINNVVRPITTIDIYSKVYGQIYNKASQV
                     ELECFENEKFYNKYMLVLDKADEKLINVIKNAYDIFFGSIAVIATFIIMYRMDKFLIFF
                     VFVPLLSNFTIGVLYNKKSFDREKEIISQKRRIDYTDRILKLKNYANDIRTTNLFELLC
                     YNYEESVAKIKKIVKKFFLKTFSLNFVNVILSFSIFSLLFTYAAFRTIVSKTMTMADLA
                     VILTVASVSSFIIIKITYVFSDIVKNLLFVQNLIEFLNYESKINENQQGITPDKNVRSI
                     EFKNVSFGYNSDSELLHNISFVIKGNSKVGLVGKNGEGKTTLLKLLFRLYDPTSGQILV
                     NGVDIKKYNLKEYRKLFASVMQDYQLFALPIEDNIKLCRDVEITVQNFNEILEYALISE
                     KINELPKRAKTIMTREFDDDGIDLSGGQKQKLAIARTFASSAFVKVFDEPTSAVDPISE
                     QRMLENICNQNNDGITILITHRLSLLVDFNQILVLNEGIIVEDGNHKELMEMNGIYKEM
                     YMRQAQNYIDGMEVV"
     CDS             10655..12466
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 186.2; E-value:
                     1.3e-56)"
                     /gene_kind="transport"
                     /locus_tag="ctg77_10"
                     /transl_table=11
                     /translation="MMEKNNNLKNLDFLFKLCFKAAPGYIISLFVFSCFARCVVFLEHV
                     YSIKYVLEVAEFNKPFNLAINIMVIVLLLLIAKLFIGAIFDNYYTLYLLPKVNMQVKLK
                     IYSHAKDMDIKNYDDPNYYNDFSLSVNEVEKSIERLINTIKNLTEGITGVICCGMLYVF
                     NDKLSMLFIVISFIGCFTIDLITTKINFKIAISRNHHERKREYCKRIFYMNEYAKEVRM
                     IPQVREIFGQKFIESNEEIYNIEKKYVNVKFWMEFIKNYIFCGICLNIWYIVYLLYKVI
                     VVNSITVSTMAILYDSGKQFRDGLNVISTTITELSENSLYIGKIKEFLGKEPTIVSSEN
                     IAVPSENVSLEFRNVYFRYNDDSPYIIRDVSFKVNVGERIAIVGDNGAGKTTLVKLMLR
                     LYDPTSGEILLNGINIKKYNVDEYRKSAGIVFQDFQVYAASLYENVSMCKDKGNNGKQK
                     ENAMSLLTKLGFKKAQYLVHGIDTSVTTEFDRMGINFSGGEKQKIATARALYGPHNFYV
                     LDEPSSALDPISEDILNTTMKNLTRNKTALFISHRLAMAKDVDKIIVMNRGEIIETGTH
                     DQLIKSRGKYAKMWDAQSKRYNISVYM"
     CDS             12476..13345
                     /locus_tag="ctg77_11"
                     /transl_table=11
                     /translation="MYEKGIVIVMKKVIIYGNCQIEYIENYLKASLGFSKKYEIVLSKP
                     IFAVSEKDVGIEKLADCDVFIYQNINGEFKHELKTENIKKLLKKECICINIPVAYFTGY
                     FPQYAHNPVNKSSEKYVWGKYPYGDSNIIEFMESPLSNKKEEREQFLKKVESRDFYNEE
                     FLKTNIKNSLEELRKRECNCDFNISDLIEASYDKEYLFFTVNHPSFKIIRYMSVQILKI
                     LGIDTYDIEDLVIEKLDHIMIPIYPSVASGLELKFIDDGQLYNYIDGKISFGHYMKDYI
                     EFCYGLSE"
     CDS             13605..14915
                     /locus_tag="ctg77_12"
                     /transl_table=11
                     /translation="MTFPETVKTTLWDIIDEMSRSLSSFVKNLDKDFIRKRKLDFKKMM
                     HLIISMESGSLNHELLKFFEYDSSVPTGSAFYQQRSKLSVSAFRHFLKEFNLKFPLEKF
                     RGKYYLIACDGSEFNIARNPNNPDTFHEPNGKSLSGFNMVHTISLYEVCSRRYLDLEVQ
                     PGRLKNEFQAICNLMDRYAYGGSPIFIADRGFSSYNVFAHAIENHVDFLIRAKDLNVQR
                     FLGVEALPDKLDTTIELILTRTQSKKKHKHPEKESQYRSICKNIAFDYLDPADISDEYL
                     LKLRIVRVEVSDGVFENIITTLSEEAFTPDDIKYCYNLRWGIETSFRDLKHTIGATNLH
                     SKKTEYVAFELWSRLILYNFCSIIILHVPVKSMDRKYEYQVNFSLAMKICFDFLRGVAP
                     PNLENLISKYILPIRPDRNYARQHRVQKPLSFSYRFV"
ORIGIN
        1 gattgatact ataaacaaag ccaaagctcg ccgaaatatc gatcaaccat taattatcca
       61 ttcggatcgt ggcagccaat atgttgcaaa ggaatataaa aaagcaaccg aaaatatgca
      121 gcgcagttat tcgaagaaag cttttccgtg ggataatgca tgcattgaat cctttcattc
      181 tgttatcaaa cgtgaatggc tcaatcgctt taaaatccgt gattataagc aagcatacca
      241 gttgattttt gaatatctgg aagctttcta caatacgaaa cgaattcata gccattgcaa
      301 ctatatgtcg ccaaacgaat tcgaacgagt gtatgaaaga acgcacactg aggctgagct
      361 tctggcaggt taaaatgggg agaaatttct cattttaact tgtactaaat cttgacatag
      421 gaccaaagct tgaatctatc agagagaatg tccgtgaggg gattgttgag acagaccaga
      481 cacttgactg tattgatggt ttcggtgagg ggatgcgaat gacgaatgaa cagctggcaa
      541 acagttttcg tgttcttgcc agaaaggagc aggtggatta ttccaagaaa gaatatgcac
      601 agtcaaatat agcagttata cgcaaacttt gggagtggca gaagaaggta tatcagaatc
      661 tggaacttca tctggatgcg gctatagata aggtagataa tctggctaag gatgtggaat
      721 tacagaggat ggagaagaaa tgggatgaac tgtatgagca gacaaatcag gaaggcaaaa
      781 atacagtgaa acaacagacg gtggcattag tatcggagaa tcaatatgag tatggtgctg
      841 aggcgtttga ggcatatatg gagaaacagg gagatacagt taatatagaa actacgttaa
      901 ttgataagaa aaataatgtg agataacaga ctagagttaa cagagggcgg aaatatcaaa
      961 attctgccca gtacatataa atggtttata tactccagat agtatttaag gcgtgggagt
     1021 aaaattctgc gtcttttttt gtgtaatcag acagtatttc agttgagttt tagtttttca
     1081 ttcaaggtaa ttacaagata gttaatagtt cagttaatag ttcgtttata aaaaaatgtt
     1141 gacaaatata ttcatatttg atatgcttat atataatatg tggcaaactt atagaaatat
     1201 aaggacgcaa agttgggaat ctaaggtgaa ttaatactat gatagttcga ctgcaatatt
     1261 ataataatat tgcagttttt ttgcgttcaa agggaggtac aaatactatg gtggaaatta
     1321 aaaccggtag ggagttattg ttggaaaacg tattatcctt cagaggcatt gttaatcaga
     1381 aggaaataca acaaattggt atggattgca ggcaattaca gttggatata gcgtaacaaa
     1441 acgaatgaat cctttagaca tagaaaatgt agaaattgaa atatatgtag gtataaatcc
     1501 gaatatatta taaaaatggt atattgtagc ttacaaaagt aagaccacta tatataaaaa
     1561 aagaggactt tgataaattc gcgaaaacca tcagagtccc aaggaataag cacttatgaa
     1621 aaagtaccta ggacaagtat atcacttttt caaataagtg taaataaaat atttattttg
     1681 aaaaggagat taaaacaatg gatctttttg ctttctctgg gcattgtgag gtttttacag
     1741 tactgtttgg tcattttaaa ccatccctct ttgctttccc taacgtagtt caaagttaat
     1801 ggcataacct tccgttccaa tgtaagccat cataggtgca tatccatcaa agcctttata
     1861 tgtgcgggaa acaccttctt tattggaatt ggagttgtcc atcggcgtga catcaatatc
     1921 tacagataca aatccattcg gcagagcagt tggctgaatc ttattggaaa ttaacagctc
     1981 gacattttca ttcaaaatgg tttcacacac gaaggctgtc accaatatca ttcatgcgct
     2041 gacacagagt ttcttcggat ggaatgctcg gggtaatgcc aagcgctgcc tagtaaaatg
     2101 ctttatcatc atccatttcg tgtactgcct caaaatatgg ttttcccatg cggagcatac
     2161 caatgtaagt gggatgatgt aaccgttttt gatctgatgc tgggaacagt ttttggttac
     2221 atccatacgg tttagttttt ggaaaaaatt attttttcca aaaagtgcat cgacaacagc
     2281 aagtccgctg tcagggataa tgcgctcatc tgtaaattcg acaataatgt tttggttcgt
     2341 attcagggta aaactccgat agttgttcat ggtaaagaac tccttcatta gtgattgata
     2401 gagtaagtat accatgaaat tcattcacac aataggtgaa agcggaagaa cttgtaaaaa
     2461 caccttgaac cttctaaaat acaaattttt gatgggctgg cttgcattga acttcacgga
     2521 tttaggtaat gtttattggg acaacaaaga aaccttggag gataaattga tagtaaatta
     2581 tttgaatgtt tcaaatataa atgctgatga tatgtacatt ttatatgatg cagcttctga
     2641 tgagcgaaaa caaaagagta aaatatataa gagaaaaatt gacgcagata tatgtatatg
     2701 ttctgaaagc ctgctcaagt atacgattaa agagattaca ggaaaaagtc ttataaagaa
     2761 agacatttcg attgcttatg aatataatga atttggaaaa ccatatttaa aaagtaacaa
     2821 tctatttttt aatatttccc attcaagtga atggatagca gttgtctgtg atttggcaga
     2881 agttggaata gatattgaaa ggattcgtcc agctgaacca cgtatgatat gcaggttgtt
     2941 ttctgatgaa gaaattggat atattaattc gggattatgt tctgaaagac atagaagatt
     3001 tacaaagctt tggacgatta aagaaagcta tgttaaatac attggaaccg gactattaac
     3061 ttcgttgaag tcatttcgag ttgatgttag caataaaaga ataatagatc tctgtgatac
     3121 agataattat gacgtgatta aatatagcaa agaaacagta ccaaattact acatgtcagt
     3181 atgtggtaag aacgagtagg cgttgataaa agagataaaa ttggaagatc tatttatgga
     3241 tttttaagaa taagaggaga tgtgaattaa aacaatgaca gagaagaaaa tcgtaggaaa
     3301 gttaaaatca ttcaatgaag taatgtataa agattgtttt tatcatgctt taataccaac
     3361 atttagacat tttacagggt ttatcgaacc actattgtta aatgacattt gtttggaata
     3421 ttctatcgag aatcaggata aggttccgac attaataatg aaaaatgcta gttctcagag
     3481 ttatagtgac atttttgaga agtttggaat ttcagtgaaa aaatcacgta tatcttcgaa
     3541 gaaaatgatt gatcatacga tagaagtctt gaataacgat gggattgcaa taatcagaat
     3601 tgattgtttt tatgaaccat taagaattga tagttatcag aaataccatg caggacatgt
     3661 aattcctata tatggatatg ataacactag gaatatgttc gatattcttg aaggtgattt
     3721 tgtagacagt gttgtatatg atgcaaaaaa acttgaatat gaacatgtaa gaaatgctta
     3781 taaggggtat atagaagaat ttaaaactca taatcacaca tattatgagt ttaaaaaggt
     3841 tgataatagc gttgattaca aaaaagaata tgaggttaga aaaaataatt ttactgagta
     3901 ttttcaaagt aattcggatg tgtttttgga atccctcaaa gagatcaata attataagca
     3961 gaaactgagt aattattttg ctaatgggat aaatgataat atatcaacaa ttgtcacttc
     4021 atttaacagg atgctggatg aagttgttgt tcagaaatat ctattctcta aactatttga
     4081 attgccatca gaatatagtg tcgttattga tgaaactatt agattatgga aagcgattag
     4141 ggcaattgtt acaaagagca tgagttctat gagactctat ctaatatcag caaactgcag
     4201 gtgcaactgg aatagcaact attaacgaac cttgacagct ggataactac tcaaaacctt
     4261 gctataggga ttttataccc ttttttcggt caaaagagat tagttgaagc tgtatggatc
     4321 aaatttaaag attcatggcg caatttctga tttagatact gtttcatgaa taattcagga
     4381 taaatatttt attagtttag atgatttgaa ttatggatat tggagcgaag aatgacatnn
     4441 nnnnnnnnta gaatatttac ataaacaaat aaaagaaaga tcagacgcaa ttgctgtaaa
     4501 aagtgaagag gcatccttaa cctatgatga gcttgataaa tatagtaatc ttgtcagaga
     4561 atctatccct tcaaagaaaa aagtagtcgc agtatacaca gaacaggtgc cagaaataat
     4621 ttcaatgata gttggaattg ttaaatctgg tgcagcattt atggtgattg ataaagaatt
     4681 tccattaaaa agaattgaat atatgttaaa taacagtgag tgcaataccg ttatttacaa
     4741 tgacttagga aatgatgaaa aacttgagaa gttaaaacag aataatgata taacgtttgt
     4801 tgattataaa aattgtctga aaggtaaatt acaaaatagt aaggaagtgt gtagaaaatg
     4861 tacggatatt cagtatattg tgtatacttc tggtacaacg ggaaatccaa agggcgtaat
     4921 ggtaacggat gagaaccttg ctaattatat ccaatggttt ataagtgaat ttgcgcttac
     4981 taatgatgaa agttctattg ttgtgtcatc tttagcattc gacctaggtt attcaaatgt
     5041 gtttcctgta ttagcggtag gtggaactgt aaatttacta aagaaaacac agtatatatc
     5101 acccgtcaag gcaattaatt atatcaagga taatgcagta acatttatga aatgtacacc
     5161 aagctatttc aatctgatga tgaattataa tgatacagga atattggact ttggagaaaa
     5221 attcaggttt ttggtacttg gtggtgaaaa tcttaaaatt aaagatgtaa gcaaatttat
     5281 aaaagataat cctaatatta gagtcataaa tcattatggt ccaactgaaa caacaatagg
     5341 tatagcattt tcagaaatat cggatttgag tctaaaaaaa tacaaagaga aaccatttgt
     5401 gggatatcca atcataaata ctgaagtatt tattaaagat gattatggag tattgcatga
     5461 ttacgaagat tgtaaaaaaa atcatatcaa aggagagttg tgtattcaga gtaaaagcac
     5521 atctgcagga tatatcaaca ataaagaatt aacaaatgaa aagtttattg taaacggtgg
     5581 tcataaagga aagatttatt gtacagggga catagtacac attgaaaatg atggtctgat
     5641 aaatattatg ggaagagagg attctcaggt caaaattaat ggctacaggg tagaagtcaa
     5701 tgaaataaaa aatataatgc ttgaatttgc gagtatcgat tttgcagaaa tcgatgtgga
     5761 agaccaagac atcattgcat atataaagga atcaaagaat atcgaatatg atgaactgta
     5821 tgatttttta ggtgaaaggt tgtcaacata tatgcttcct aaaaggatat atgttgttaa
     5881 aaaaattgct ttaacactta atggaaagat tgactttaag attatgaaaa aatcaggcaa
     5941 attattacag tcaaacaatc caatcacaaa gccggaaacg gatatggaaa aggcgttgta
     6001 taaactgtgg aaacaacttt taaattgtga agaggaattc agtatagagg ataacttctt
     6061 ttcggttggt ggaacaagta ttttgggaat tgaattactg tcgtatctta atctggataa
     6121 ttttgaatac caggatttat tggaaaattc atctattaag aagctggctt gtgttattga
     6181 aatgaagaaa ggaagtatca aagaaataga cgaattgaaa atgccagact gtttgacgga
     6241 ttatgttgac aagaaggaag tcagatatat ggtgaaaccg tttaataaag tatattatga
     6301 agattgcata tataatgcga ttttttcggc atttgactat tttaatattg aactcagata
     6361 ttttctctct catgaacttc ctacgttaga tgaaagtaat tttattttca aacgagataa
     6421 caatatggtt aaaattgatt gccatattaa taaagtttat ggcgaggact ttattaaagg
     6481 agattttgtt ggaaatagca atgttatcga taagataaga actgcattat taaataagaa
     6541 tatcccaatt gttcgtatcg attgttatta cgagaagtat agaaaagaat tttataataa
     6601 gagtcatttt atgcatgcat tatgtattac tggtttcaat gatttcgaaa agaaactata
     6661 tgcattagag catagtaatg tgaactcgct agactataag gagatagaga tttcgtatca
     6721 agaagttata aatgctaatt ttgcatatga gtattttact ggttatacca gagaatctta
     6781 ttacgaagtt tctaagataa aggatagttc gtatttacaa aaagatcctg tgcgggattt
     6841 tgtgcagtat atatgtaagc acaaatctgt gctcaaaaaa aatatcagta aaactaatgt
     6901 ggtaatggtt gagggtaagt ttgcattgga caatactgaa ttgttcagtg ataggttgat
     6961 acagatgagg atatttctaa agatggaaat atttaaggcg gaattattgg ttgatgatga
     7021 acaggtaaaa aatcctatta tcgaatctaa taaaatattt gaaaaaatgt ttcaaattgt
     7081 tttggttaaa aaaatgcgaa agcagtcatt ggatttaact gaaaccgata agtctaatat
     7141 gaaatatgca ttaacattgc taagagagta tttttctaaa ctgcttaaat gggcagataa
     7201 caaggtagag gaaatgagta caaacaataa ggagattaaa attttggaaa atagaataaa
     7261 agagttattt tatcaggtga caaatattga tcaggatatt gatgttaaca gaaaattaac
     7321 tgattacggc ataaattcaa ttacaataat tgagtttctt gtaaaaatcg aggatgaatt
     7381 tgatattgaa tttgatattg aaaatatgac tgtagagaaa ttcgagtcat tacatgacct
     7441 atgcgattat gtgcaatcga atagccaaaa tttagataat tgcaaaactt actaagcttg
     7501 ttcgacatct tgattaaaaa catggaagaa ttcaagtgtt gctcgtattt tgggataaat
     7561 gtgggtaaaa gtttttgaac atcatcaaaa atatctgccg atgagaactg tttgtatggt
     7621 accatataaa tatatctcct ttaggtggac ggttaatagc ttcaagaaaa ctctatttta
     7681 ccataatcca agagaatata ttttattata gtaacaaaaa atgccgtata tatgcgtgct
     7741 ataggtgttt tgcaaatgcc taaatagata aaattgataa agaggagaaa gattatgtca
     7801 aataaaacaa cggttgcgac aatatttgca ttaaattatt acagtaaagg aataacaatg
     7861 ttagagagtg taaataaatt taatcctgat attgaattaa acatattgat atcagataag
     7921 gtagaaaaca aggaaaaatt tcttaatgat gcacttatta aaaagcttaa tgtcaaagtt
     7981 acatttcttg aggatttgga attaagggat ttcgagaatc ttgcttttaa atacgatatg
     8041 gttgagtttt gtacatgtgt taaaccaaaa tatcttttaa tgttagcaga tgagtacgat
     8101 aagattattt atcttgatcc ggatattatg gtatatgcag tacttgaaga aattcaaaat
     8161 cttttagatg ttcataatat agtgttaaca ccacatattt caggaacaat cgaagagggt
     8221 atttctccgg cgactgattt tcttagaaat ggtatatata acctgggatt tattgcagta
     8281 aaaaatgcag atgtggtaaa gatattttta aaatggtggc ataaaaaatt aatgaactat
     8341 tgtttctgtg attctaggaa atctttggca tgggatcaga aatgggttga ttttgtacca
     8401 gcattatttg atggagtgta tgttttaaga agtccaaatt ataatgtggc attctggaat
     8461 attcagggac gatatatttc tgaaaaaaat agtacatatt atgtaaataa tgattatcca
     8521 ttggtttttg ttcattatag tcactttaat attaattcac ctaattattt attctacata
     8581 aatgatgggc tcaacttatt gatagaggac agaagagact taagtaaaat cactagtgaa
     8641 tatgtgtctg cattaagaag caatactaat gaatcatatg ctaaaaagaa atacagatac
     8701 aactattatg ataatggtga aattgtcaaa gaagcgcata gaaaattata taatcacctt
     8761 gatacagaaa cggaagaata taacgaacct ttcaatacaa aaaagacaaa ttcattctac
     8821 aagctggcaa agatgtttga ggatatcgat gtgtcagcat tgtaataaag gagagaggaa
     8881 atatgaagtt tttggaaaaa gttaaatata taaaagaatt taataaaaag cgttttatat
     8941 atgaaggaat atactgcttt atagaatatc ttgaatatat tttggttggt tgtctgtatc
     9001 tgaagtggat cgtaacagat gtacagagtg gaaaagaatt atcatatatt ttaaaaaaaa
     9061 tacttggctt tacggtaatt ctgtttttaa tcttcattat cagaagttat ataaataatg
     9121 ttgtgagacc tataacaaca attgatattt acagtaaagt atatgggcaa atatataata
     9181 aggcttcaca agttgaatta gaatgttttg aaaatgaaaa gttttataac aaatacatgc
     9241 tggttctaga taaggccgat gagaagctaa taaatgtaat taagaatgct tatgatatat
     9301 tctttggatc aatcgctgta attgctacat ttatcataat gtatcgaatg gataagtttt
     9361 tgatattttt cgtatttgtt ccattgctct ccaactttac gattggcgta ctttacaata
     9421 aaaaatcctt cgatagagaa aaagaaatta tcagtcaaaa gcgaagaatt gactatacag
     9481 acagaatatt gaagttgaaa aactatgcta atgatatcag aacaactaat ttgtttgaac
     9541 tattgtgtta taactacgaa gaaagcgtgg caaaaattaa aaaaatagta aaaaaattct
     9601 tcctgaaaac atttagttta aattttgtaa atgtgatttt atcattctcg attttttcat
     9661 tgttgtttac atatgcagct tttagaacga tagtatcaaa gacaatgact atggcagatc
     9721 ttgcagttat ccttacagtt gcgtctgttt cttcatttat tatcataaag attacatatg
     9781 tattttcaga tattgtaaaa aatcttttat ttgttcagaa tctaattgaa tttcttaact
     9841 atgaatcaaa gattaacgaa aaccagcagg gaataacccc tgataagaac gtaagatcca
     9901 ttgaatttaa aaatgtcagt tttgggtata acagtgattc cgagctttta cataatattt
     9961 catttgttat taagggaaac agcaaagtag gtctggttgg taagaatggt gaaggaaaaa
    10021 caacattatt aaaattacta ttcagattat atgatccaac atctggtcag attttggtga
    10081 atggcgtaga tattaagaaa tataatttga aagagtatag aaaattattt gcatctgtaa
    10141 tgcaggatta tcaattattt gcattaccta ttgaggacaa catcaaattg tgcagagacg
    10201 tagagattac agtgcagaat tttaatgaga ttcttgaata tgcattgatt agtgaaaaaa
    10261 ttaatgaact tccaaagcgt gccaagacta ttatgactag agaatttgat gatgatggaa
    10321 tagatttgtc tggtggacaa aagcaaaagt tagcgatagc tagaacattt gctagttcag
    10381 cgttcgtaaa ggtatttgat gagccaacaa gtgctgtgga tcctatctcc gaacagagaa
    10441 tgttggaaaa tatatgtaat cagaacaatg atggcattac tatattaatc acacatagat
    10501 tgtcgttatt agtagacttt aaccagatat tggttttaaa tgaaggaata atagttgagg
    10561 atggaaatca caaggaactc atggaaatga atggaatata caaagaaatg tatatgcgcc
    10621 aggcacaaaa ttatatagac ggaatggagg tggtatgatg gagaaaaata acaatttaaa
    10681 aaatttagat tttttgttca agctatgttt taaggcagct ccaggatata taatttcttt
    10741 atttgtcttt tcatgttttg cacgttgtgt tgtatttttg gaacatgttt attcgattaa
    10801 atatgtttta gaagttgcag aatttaataa accttttaat ttagctataa atataatggt
    10861 catagttttg ctcctgttga tagcaaagtt gtttatcgga gctatatttg ataattatta
    10921 cactttgtat ctgttaccca aggtaaatat gcaggtcaaa ttaaaaatat attcacatgc
    10981 caaggatatg gatattaaaa actatgatga tccaaattat tataatgatt tttcgttgtc
    11041 ggttaatgaa gtggaaaaat ctattgaaag acttataaat actattaaga atctcacgga
    11101 aggtattact ggtgttatat gttgtggaat gctgtatgtt tttaatgaca aattatctat
    11161 gttgtttatt gttatctcat ttattggatg ttttactatt gatcttataa ctacaaaaat
    11221 caattttaaa attgcaatta gcagaaatca tcatgaaaga aaacgagaat attgcaagcg
    11281 tatcttttat atgaatgagt atgctaaaga agtgagaatg attcctcagg tcagagagat
    11341 atttggacaa aaatttattg aatcaaacga agagatatac aacattgaaa agaaatatgt
    11401 aaatgttaag ttctggatgg aatttataaa gaattatata ttttgcggaa tatgtttgaa
    11461 tatttggtat atcgtttatc tgttatataa agttatcgtt gtgaattcta taactgtaag
    11521 cacaatggct attctttatg attcaggaaa acagttcaga gatggactaa atgttatatc
    11581 aacaaccata acagaattat ctgaaaacag cttgtatatt ggcaagatta aagaattttt
    11641 aggtaaagag cctactatag tcagcagtga aaatatagcc gtaccttctg aaaatgtgag
    11701 tttagaattt agaaacgttt attttagata taacgacgat tcaccatata tcataagaga
    11761 tgtttccttt aaggtaaatg taggcgaaag aattgcaatt gttggagaca atggggcggg
    11821 caagacaaca cttgttaaac tgatgcttcg attatatgat ccgactagtg gtgagattct
    11881 gttaaacggc ataaatatta agaaatataa cgttgatgaa tatagaaaat ctgcaggaat
    11941 tgtgtttcag gattttcagg tatatgcagc gtctttatat gaaaatgttt caatgtgtaa
    12001 agataagggc aacaatggaa agcaaaaaga aaatgcgatg agtctattga ctaaacttgg
    12061 atttaaaaag gcacagtatc tagtacatgg tatagatacc agcgttacaa ccgaatttga
    12121 taggatgggg ataaatttct cgggcggtga gaagcagaaa atcgcaactg ccagagccct
    12181 atacggtcca cataattttt atgttctgga tgaaccatcg agtgcattag accctatatc
    12241 ggaagatatc ttgaatacta caatgaaaaa tttgactaga aataagacag cactatttat
    12301 atctcacaga ttagcgatgg ctaaagatgt agacaaaata attgtgatga atcgaggcga
    12361 aatcatagaa actggaacac atgatcagct tataaaaagt cgtgggaaat atgcaaagat
    12421 gtgggatgcg cagagtaaac gatacaatat tagtgtatac atgtaattaa ggtacatgta
    12481 tgagaaaggc atagttatag ttatgaaaaa agttattata tatgggaatt gtcagataga
    12541 gtatattgaa aactatttga aagcttcatt gggattttct aaaaaatatg aaatagtctt
    12601 atcaaaaccg atatttgcag ttagtgaaaa agatgtagga atagaaaagc ttgcagattg
    12661 tgatgtattt atttaccaga atattaatgg agaatttaag catgagctga aaactgaaaa
    12721 cataaaaaaa ttactgaaaa aggaatgtat atgcattaat attccggtgg cgtacttcac
    12781 aggttatttt ccacagtatg cgcataatcc agtcaataaa tcatcagaaa agtatgtttg
    12841 gggaaaatat ccatatggtg actcgaatat aattgagttt atggaatctc cgctaagtaa
    12901 taaaaaggaa gagagagaac agtttttgaa gaaggttgaa agtagggatt tttataatga
    12961 agaattttta aagacaaata ttaaaaattc attagaggaa cttagaaaac gcgagtgcaa
    13021 ttgtgatttt aatatatctg acttgataga agcatcatat gataaggaat atttattctt
    13081 cacagtgaat catccaagtt ttaaaattat aagatacatg agtgtacaaa tattgaaaat
    13141 attaggcata gatacatacg atattgagga tttggtaata gaaaaactgg atcatataat
    13201 gattcctatt tatccaagtg tggcaagtgg tctggaacta aaatttatag atgatggtca
    13261 attgtataat tatatagatg gtaaaatatc gtttggacac tatatgaaag attatataga
    13321 gttttgctac ggtttgtcgg aataattgtg aaatttatct tgcataattg caacttttaa
    13381 cacattatat gaaaacatat cccaaaagta actgttcaga accaatgtca cttagttaag
    13441 cttattggtt cactatgagg caaacctcta acaaaaattt ataaattata gttgacacat
    13501 caatcaaaat gtgcggccgc tctcgttgct gatatatttt taaggtaacg agagcggctc
    13561 ttgaattaga actatatttc attctgattc aaggaggttt acctatgaca tttcctgaaa
    13621 ctgtcaaaac tacactttgg gacattattg atgagatgtc ccgctcactt tcttcttttg
    13681 taaaaaatct agataaggat tttatccgca aacgcaaact tgattttaag aaaatgatgc
    13741 atctgatcat ttctatggaa agcggaagcc taaatcatga acttctcaaa ttttttgagt
    13801 atgattcctc agttcctact ggctctgctt tttatcaaca acgttccaaa ctttccgttt
    13861 ctgcattccg tcactttctc aaggaattta accttaaatt ccccttagaa aaatttcgcg
    13921 gaaaatacta tcttattgcg tgtgatggtt ctgaatttaa cattgccaga aatcctaaca
    13981 atcccgacac cttccacgag cctaatggca aatctctatc cggttttaat atggttcata
    14041 ccatctcttt gtatgaggta tgttctaggc ggtatcttga tttagaagtt cagcctgggc
    14101 gtcttaaaaa tgagtttcag gctatctgca atcttatgga tcggtatgcc tatgggggat
    14161 cccccatttt cattgctgac agaggatttt ccagttacaa tgtttttgcc catgcaatcg
    14221 aaaatcatgt tgattttttg attcgtgcta aggatttgaa tgtacagcgt tttcttggag
    14281 tcgaggctct tcctgacaaa ctggatacaa ccatagaact gattctgacc agaacacaat
    14341 caaaaaagaa acataaacat cccgaaaaag aatcacaata ccgctctatt tgtaaaaaca
    14401 tagctttcga ctatcttgat ccggctgata tttccgatga atatctgctt aaactaagaa
    14461 tcgtccgcgt tgaagtgtcc gatggtgttt ttgaaaatat catcactaca ctttccgaag
    14521 aagcttttac accggacgat ataaaatact gttacaatct ccgctggggc atcgaaactt
    14581 cttttcgcga cctgaagcat accattggtg ctactaattt acactctaaa aaaacagagt
    14641 atgttgcatt tgaactgtgg agcaggctca ttttgtacaa tttctgttcc ataattattt
    14701 tacatgtacc agtaaaaagt atggatcgca aatatgagta ccaagtcaac ttttctctcg
    14761 caatgaaaat ctgttttgat tttctaagag gagtcgcacc accaaatcta gaaaacctga
    14821 taagtaagta tatcctacct atcaggcccg atagaaacta tgcccgccag catagagtcc
    14881 aaaaaccgct cagcttctct taccgttttg tataacatag agtagctgac aattctatta
    14941 tacactaatt ttaaggcaga cgaaagtctg tcttaaagtc gcgcctgata tttatatgat
    15001 tttttttgaa cgaagattgt tttaatcttt tttgtttacc acatcctttt cacgctagat
//
